##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488846
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.425712801168466	33.0	31.0	34.0	31.0	34.0
2	32.50695924687938	34.0	31.0	34.0	31.0	34.0
3	32.50423241675293	34.0	31.0	34.0	31.0	34.0
4	36.022864051255404	37.0	35.0	37.0	35.0	37.0
5	36.08822205766233	37.0	35.0	37.0	35.0	37.0
6	36.0751218175049	37.0	35.0	37.0	35.0	37.0
7	36.236235951608485	37.0	37.0	37.0	35.0	37.0
8	36.08864550390103	37.0	37.0	37.0	35.0	37.0
9	37.99566530154691	39.0	38.0	39.0	35.0	39.0
10	37.66267086158013	39.0	37.0	39.0	35.0	39.0
11	37.673416576999706	39.0	37.0	39.0	35.0	39.0
12	37.57264660036085	39.0	37.0	39.0	35.0	39.0
13	37.555344627960544	39.0	37.0	39.0	35.0	39.0
14	38.815894985332804	40.0	38.0	41.0	35.0	41.0
15	38.888895480376235	40.0	38.0	41.0	35.0	41.0
16	38.86624212942317	40.0	38.0	41.0	35.0	41.0
17	38.8415656464408	40.0	38.0	41.0	35.0	41.0
18	38.74695507378602	40.0	38.0	41.0	35.0	41.0
19	38.66891413655834	40.0	38.0	41.0	35.0	41.0
20	38.59968988188509	40.0	38.0	41.0	35.0	41.0
21	38.497545239195986	40.0	37.0	41.0	35.0	41.0
22	38.50475609905778	40.0	37.0	41.0	35.0	41.0
23	38.48461274102683	40.0	37.0	41.0	35.0	41.0
24	38.42583553920867	40.0	37.0	41.0	35.0	41.0
25	38.24497899133878	40.0	37.0	41.0	34.0	41.0
26	38.273791746275926	40.0	37.0	41.0	34.0	41.0
27	38.281464919422476	40.0	37.0	41.0	34.0	41.0
28	38.20436497383634	40.0	37.0	41.0	34.0	41.0
29	38.174862840240074	40.0	37.0	41.0	34.0	41.0
30	38.029185060325744	40.0	36.0	41.0	34.0	41.0
31	37.989229736972376	40.0	36.0	41.0	34.0	41.0
32	37.88248650904375	40.0	36.0	41.0	34.0	41.0
33	37.7774411573379	40.0	36.0	41.0	34.0	41.0
34	37.664299186246794	40.0	36.0	41.0	33.0	41.0
35	37.559769334309784	40.0	36.0	41.0	33.0	41.0
36	37.45698236254362	40.0	36.0	41.0	33.0	41.0
37	37.40896724121707	40.0	35.0	41.0	33.0	41.0
38	37.30786791750367	40.0	35.0	41.0	33.0	41.0
39	37.23669621925924	40.0	35.0	41.0	33.0	41.0
40	37.157595643617825	40.0	35.0	41.0	33.0	41.0
41	37.06397924908867	40.0	35.0	41.0	32.0	41.0
42	36.95883161568265	39.0	35.0	41.0	32.0	41.0
43	36.837543929990225	39.0	35.0	41.0	32.0	41.0
44	36.861246691187	39.0	35.0	41.0	32.0	41.0
45	36.83997823445421	39.0	35.0	41.0	32.0	41.0
46	36.74965735630444	39.0	35.0	41.0	32.0	41.0
47	36.683090380201534	39.0	35.0	41.0	32.0	41.0
48	36.62117313018824	39.0	35.0	41.0	32.0	41.0
49	36.562565715992356	39.0	35.0	41.0	32.0	41.0
50	36.476557034321644	39.0	35.0	41.0	31.0	41.0
51	35.6366749446656	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	4.0
10	8.0
11	10.0
12	11.0
13	14.0
14	27.0
15	27.0
16	31.0
17	73.0
18	133.0
19	166.0
20	311.0
21	466.0
22	687.0
23	1024.0
24	1613.0
25	2474.0
26	3516.0
27	4310.0
28	4436.0
29	4728.0
30	5411.0
31	6674.0
32	8537.0
33	12311.0
34	25197.0
35	36483.0
36	32544.0
37	49902.0
38	93565.0
39	193887.0
40	256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.67253900001227	19.883971639330177	24.656231205737594	12.787258154919954
2	34.70111241577102	24.523469558920397	23.540337856911993	17.235080168396593
3	33.138248037214176	23.013791664450565	26.712707069301988	17.135253229033275
4	29.932944117370297	25.39081837633938	26.038670665199266	18.63756684109106
5	25.56142425221849	29.711197391407516	24.59997627064556	20.12740208572843
6	26.108631348113718	34.61580947783147	24.393367236307547	14.882191937747265
7	78.52043383805943	6.734227139017196	9.982489372931353	4.762849649992022
8	78.61146455120836	6.687586683740891	9.876934658358664	4.824014106692087
9	71.77638765582617	8.944330116232923	12.0215773474673	7.257704880473605
10	38.312474685279206	25.26378450473155	20.13619831194282	16.28754249804642
11	31.10631978168994	24.388457714699516	24.26019646268968	20.245026040920862
12	26.990913293757135	22.369007826595695	29.989403615862663	20.650675263784503
13	25.081109388232697	23.756970497866405	31.420938291404653	19.740981822496245
14	21.750612667384004	26.808647304058947	29.87873481628161	21.56200521227544
15	21.508614164788092	24.218874655822077	33.050081211669934	21.222429967719894
16	23.72955900222156	23.638119162271963	31.201646326245893	21.430675509260585
17	23.592910650798	23.27195067567291	28.875965027841076	24.25917364568801
18	24.244240517463577	23.87173056545415	29.52934052851	22.35468838857227
19	25.09931553086248	25.07885919082901	27.909812088060455	21.912013190248054
20	25.399410039153437	25.34908744267111	28.36660216100776	20.884900357167695
21	25.4192526889859	25.47796238488195	27.950520204727052	21.152264721405107
22	23.934326965956558	24.752785130695557	27.241298895766764	24.07158900758112
23	22.735994566796087	25.845767378683675	28.322416466535472	23.095821587984762
24	23.244334616627732	25.14227384493276	28.807436288728965	22.805955249710543
25	23.841250618804285	25.561219688818156	27.63835645581635	22.959173236561206
26	22.033114723246175	26.13522459015723	28.278844462264193	23.552816224332407
27	23.24167529242338	25.17070815757928	29.468380635210274	22.11923591478707
28	21.456245934302416	25.64652262675771	30.188239240987958	22.70899219795191
29	22.91969250029662	25.605405383290442	28.92383286351939	22.551069252893548
30	23.215082050379873	25.398182658751427	28.81561882474235	22.571116466126345
31	24.546585223158214	26.67424915003907	26.474595271312438	22.304570355490277
32	24.644571091918518	25.962368516874434	27.05985115966992	22.33320923153713
33	23.912643245521085	25.848426702888027	26.39317903797924	23.84575101361165
34	22.9732881111843	25.33517713144835	27.958907304140773	23.73262745322658
35	24.354909317044633	24.649276050126215	28.068144159919484	22.92767047290967
36	23.871526002053816	26.77837192080942	27.6003076633541	21.749794413782663
37	23.889323017882933	27.75229826980276	27.319851241495275	21.038527470819034
38	23.48408292181996	27.989591814190973	25.733052945099278	22.793272318889795
39	24.446144593593893	25.24496467190076	26.969638700122328	23.339252034383016
40	24.509354684297303	24.092454474415256	28.847939841995228	22.55025099929221
41	22.679739631704056	24.974122729857665	28.102919937976377	24.243217700461905
42	22.399487773245564	25.610724031699146	29.148443477086854	22.84134471796844
43	23.10441325079882	24.091840784214252	29.14435220908016	23.65939375590677
44	23.145325930865752	24.994374506490797	28.410583292079718	23.449716270563737
45	23.72219471980951	24.4060501671283	27.161110042835574	24.710645070226615
46	23.688646322154625	25.81978782684117	27.64326597742438	22.84829987357982
47	23.42046370431588	24.455349946608955	29.57229884258028	22.55188750649489
48	22.184696202894163	24.774468851131033	29.41560327792393	23.625231668050876
49	22.943830981536106	24.21273775381204	30.088821428425312	22.754609836226543
50	22.073822839912776	24.510377501298976	29.63182679207767	23.783972866710577
51	22.629826162022397	24.16323341093105	27.82328176971889	25.38365865732767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	250.0
1	661.5
2	1073.0
3	2932.0
4	4791.0
5	3224.5
6	1658.0
7	1603.5
8	1549.0
9	1536.5
10	1524.0
11	1542.5
12	1561.0
13	1580.0
14	1599.0
15	1524.5
16	1450.0
17	1443.5
18	1437.0
19	1408.0
20	1379.0
21	1466.5
22	1554.0
23	1785.5
24	2017.0
25	2637.5
26	3707.5
27	4157.0
28	4873.0
29	5589.0
30	6318.5
31	7048.0
32	8473.0
33	9898.0
34	11222.5
35	12547.0
36	12438.5
37	12330.0
38	13305.0
39	14280.0
40	16776.0
41	19272.0
42	21482.0
43	23692.0
44	26234.5
45	28777.0
46	33199.0
47	37621.0
48	40814.0
49	44007.0
50	43710.0
51	43413.0
52	39300.5
53	35188.0
54	31926.5
55	28665.0
56	27331.5
57	25998.0
58	25191.5
59	24385.0
60	22869.5
61	21354.0
62	19652.5
63	17951.0
64	16294.0
65	14637.0
66	12634.0
67	10631.0
68	9220.0
69	7809.0
70	6481.5
71	5154.0
72	4561.5
73	3969.0
74	3233.5
75	1891.5
76	1285.0
77	1039.5
78	794.0
79	577.5
80	361.0
81	284.5
82	208.0
83	148.5
84	89.0
85	96.0
86	103.0
87	60.0
88	17.0
89	11.5
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	4.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	488846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.310889627795202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.363088861991	21.933895996221455
2	8.553598043915477	5.185343324592939
3	3.2242946725938317	2.9319371984543907
4	1.849947947943512	2.242942722691281
5	1.3806080362992355	2.09237289037566
6	1.0898519895930032	1.9820630020311913
7	0.9313975816893878	1.9762042508727682
8	0.8052365035753699	1.9525947827315804
9	0.7647535064151344	2.086232321287666
>10	8.887317985612903	51.03064883313932
>50	0.10038878196799601	2.1176753302728013
>100	0.043411365175349624	2.7402307574636793
>500	0.005426420646918703	1.1231903232310985
>1k	6.783025808648379E-4	0.6046682666342048
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2941	0.601620960384252	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	944	0.19310784991592445	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	905	0.18512987730287248	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC	770	0.15751381825769262	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	638	0.13051144941351672	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	576	0.11782851859276745	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	564	0.11537375778875147	Illumina Single End Adapter 1 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	534	0.10923685577871149	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	532	0.10882772897804216	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12232891340013011	0.0
2	0.0	0.0	0.0	0.5881197759621639	0.0
3	0.0	0.0	0.0	0.8483244211878588	0.0
4	0.0	0.0	0.0	1.1872859755424	0.0
5	0.0	0.0	0.0	2.1526206617216874	0.0
6	0.0	0.0	0.0	2.718443027047373	0.0
7	0.0	0.0	0.0	3.2443755293077983	0.0
8	0.0	0.0	0.0	4.042377354013329	0.0
9	0.0	0.0	0.0	4.432479758451537	0.0
10	0.0	0.0	0.0	5.285304574446759	0.0
11	0.0	0.0	0.0	6.334101127962589	0.0
12	0.0	0.0	0.0	7.169538054929364	0.0
13	0.0	0.0	0.0	7.503794651076208	0.0
14	0.0	0.0	0.0	7.642897763303781	0.0
15	0.0	0.0	0.0	7.914557958948217	0.0
16	0.0	0.0	0.0	8.459923984240435	0.0
17	0.0	0.0	0.0	9.088547313468863	0.0
18	0.0	0.0	0.0	9.810246989849563	0.0
19	0.0	0.0	0.0	10.231647594538975	0.0
20	2.0456340033466573E-4	0.0	0.0	10.62604583038421	0.0
21	2.0456340033466573E-4	0.0	0.0	11.069130155509097	0.0
22	2.0456340033466573E-4	0.0	0.0	11.555581921504933	0.0
23	2.0456340033466573E-4	0.0	0.0	12.038351546294743	0.0
24	2.0456340033466573E-4	0.0	0.0	12.460774967985827	0.0
25	2.0456340033466573E-4	0.0	0.0	12.833898610196258	0.0
26	4.0912680066933145E-4	0.0	0.0	13.170200840346448	0.0
27	4.0912680066933145E-4	0.0	0.0	13.490956252071204	0.0
28	4.0912680066933145E-4	0.0	0.0	13.831145186827754	0.0
29	4.0912680066933145E-4	0.0	0.0	14.189744827614422	0.0
30	4.0912680066933145E-4	0.0	0.0	14.585165880461332	0.0
31	4.0912680066933145E-4	0.0	0.0	14.954402818065404	0.0
32	4.0912680066933145E-4	0.0	0.0	15.317911980460105	0.0
33	4.0912680066933145E-4	0.0	0.0	15.673443170241754	0.0
34	4.0912680066933145E-4	0.0	0.0	16.03142912082742	0.0
35	4.0912680066933145E-4	0.0	0.0	16.41334898925224	0.0
36	4.0912680066933145E-4	0.0	0.0	16.762129586822844	0.0
37	4.0912680066933145E-4	0.0	0.0	17.125843312617878	0.0
38	4.0912680066933145E-4	0.0	0.0	17.47094176898246	0.0
39	4.0912680066933145E-4	0.0	0.0	17.848361242599918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTC	25	3.8875038E-5	45.000004	18
ACGTGCG	25	3.8875038E-5	45.000004	1
CAACGCG	20	7.029304E-4	45.0	1
TCGTAAG	20	7.029304E-4	45.0	1
ATCGGTT	20	7.029304E-4	45.0	34
CGCGTAA	20	7.029304E-4	45.0	31
TCGCGTA	20	7.029304E-4	45.0	30
GCGTAAC	20	7.029304E-4	45.0	32
TCTAGCG	30	2.162933E-6	44.999996	1
GCTACGA	125	0.0	44.999996	10
CGAATAT	140	0.0	43.392857	14
TACGAAT	135	0.0	43.333332	12
CTACGAA	140	0.0	41.785713	11
CATATTA	55	6.002665E-11	40.909092	28
CGTTTTT	1360	0.0	40.53309	1
AAGCTAC	150	0.0	39.0	8
GGATCGC	35	6.2409254E-6	38.571426	8
GACGCAT	35	6.2409254E-6	38.571426	25
CGCGACC	100	0.0	38.250004	10
AGCTACG	150	0.0	37.5	9
>>END_MODULE