FastQCFastQC Report
Sat 18 Jun 2016
SRR3551465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388573
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18370.4727554410625545No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC11770.30290318678858286No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC5670.14591852753536658No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT5520.14205824902913997No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG5420.13948473002498885No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC4870.1253303755021579No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC4710.12121274509551616No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4300.11066131717849671No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4070.10474222346894921No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA4020.10345546396687366No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT3970.10216870446479812No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3950.1016540006639679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAG302.161778E-645.0000041
CGGAATC207.027516E-445.019
GACCGTT351.209155E-745.01
CGACAAT253.8860235E-545.020
CGCTAGG207.027516E-445.02
CGAATAT406.7957444E-945.014
TTAACGG253.8860235E-545.02
TCATCGA253.8860235E-545.016
TTCATCG253.8860235E-545.015
CCGTTAG253.8860235E-545.01
GCACTAG207.027516E-445.01
CCTCGTG650.044.99999615
CGAGGGT603.6379788E-1241.2500044
CATACGA556.002665E-1140.90909218
ACATACG556.002665E-1140.90909217
CGAGATA950.040.26315719
CGTATGG451.9226718E-840.02
ACGTAGG451.9226718E-840.02
ACGGGCT1200.039.3750045
GCGATAC403.45035E-739.3759