##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388573
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2468056195361	33.0	31.0	34.0	30.0	34.0
2	32.31289873460071	34.0	31.0	34.0	30.0	34.0
3	32.32928175658113	34.0	31.0	34.0	30.0	34.0
4	35.89973312607927	37.0	35.0	37.0	35.0	37.0
5	35.96224132917109	37.0	35.0	37.0	35.0	37.0
6	35.93917487833689	37.0	35.0	37.0	35.0	37.0
7	36.123580382579334	37.0	35.0	37.0	35.0	37.0
8	35.943989932393656	37.0	35.0	37.0	35.0	37.0
9	37.853250225826294	39.0	38.0	39.0	35.0	39.0
10	37.47620395652812	39.0	37.0	39.0	35.0	39.0
11	37.48256569550638	39.0	37.0	39.0	35.0	39.0
12	37.40802629107014	39.0	37.0	39.0	35.0	39.0
13	37.39233554570184	39.0	37.0	39.0	35.0	39.0
14	38.6262864378122	40.0	38.0	41.0	35.0	41.0
15	38.7203331162999	40.0	38.0	41.0	35.0	41.0
16	38.69767585498735	40.0	38.0	41.0	35.0	41.0
17	38.665836277867996	40.0	38.0	41.0	35.0	41.0
18	38.58755497679972	40.0	38.0	41.0	35.0	41.0
19	38.50820566534473	40.0	38.0	41.0	34.0	41.0
20	38.44745517573274	40.0	37.0	41.0	34.0	41.0
21	38.33561776036935	40.0	37.0	41.0	34.0	41.0
22	38.36581028532605	40.0	37.0	41.0	34.0	41.0
23	38.3301310178525	40.0	37.0	41.0	34.0	41.0
24	38.27764152424383	40.0	37.0	41.0	34.0	41.0
25	38.108553605114096	40.0	37.0	41.0	34.0	41.0
26	38.13932774536573	40.0	37.0	41.0	34.0	41.0
27	38.126835884119586	40.0	37.0	41.0	34.0	41.0
28	38.04817884927671	40.0	37.0	41.0	34.0	41.0
29	38.00193013925311	40.0	37.0	41.0	34.0	41.0
30	37.84651790011144	40.0	36.0	41.0	33.0	41.0
31	37.815164718083864	40.0	36.0	41.0	33.0	41.0
32	37.70037547642271	40.0	36.0	41.0	33.0	41.0
33	37.64019888154864	40.0	36.0	41.0	33.0	41.0
34	37.51448762523387	40.0	36.0	41.0	33.0	41.0
35	37.42256409992459	40.0	36.0	41.0	33.0	41.0
36	37.33689679931442	40.0	36.0	41.0	33.0	41.0
37	37.27883049002375	40.0	35.0	41.0	33.0	41.0
38	37.17432503030319	40.0	35.0	41.0	32.0	41.0
39	37.115128946169705	40.0	35.0	41.0	32.0	41.0
40	37.01614111119404	39.0	35.0	41.0	32.0	41.0
41	36.92172899300775	39.0	35.0	41.0	31.0	41.0
42	36.83176648917964	39.0	35.0	41.0	31.0	41.0
43	36.69370491516395	39.0	35.0	41.0	31.0	41.0
44	36.71179418024413	39.0	35.0	41.0	31.0	41.0
45	36.65599771471513	39.0	35.0	41.0	31.0	41.0
46	36.567162412210834	39.0	35.0	41.0	31.0	41.0
47	36.48941897661443	39.0	35.0	41.0	31.0	41.0
48	36.423933212034804	39.0	35.0	41.0	31.0	41.0
49	36.35944597282879	39.0	35.0	41.0	31.0	41.0
50	36.22521894212928	38.0	35.0	40.0	31.0	41.0
51	35.304002079403354	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	9.0
11	7.0
12	11.0
13	9.0
14	19.0
15	24.0
16	30.0
17	50.0
18	106.0
19	155.0
20	282.0
21	449.0
22	628.0
23	991.0
24	1383.0
25	1981.0
26	2709.0
27	3434.0
28	3783.0
29	4375.0
30	4961.0
31	6191.0
32	7945.0
33	10852.0
34	20137.0
35	28905.0
36	28198.0
37	43188.0
38	77796.0
39	139809.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.85334801954845	19.798596402735136	23.79166848957596	11.556387088140452
2	33.8829512086532	24.036152794970313	25.056553080116224	17.024342916260267
3	33.84228960838762	23.54924299938493	26.10577677810862	16.50269061411884
4	29.55223342846775	25.405007553278274	25.474235214489944	19.56852380376403
5	25.152287987070643	30.3621198590741	24.2862988421738	20.19929331168146
6	25.14971446806649	36.19345657058005	24.211666791053418	14.445162170300046
7	78.45527095294835	7.311882194594066	9.767276676454618	4.465570176002965
8	78.56413080682394	7.330668883324369	8.983382787790196	5.121817522061492
9	72.36735439672854	8.871434711109623	11.547379771625923	7.21383112053591
10	38.40899908125371	25.20350101525325	20.286535605922182	16.100964297570854
11	31.245094229398333	25.04239872559339	23.183288596994643	20.529218448013626
12	26.713127263088275	23.101708044563054	29.661093282343344	20.524071410005327
13	25.294603588000196	24.359129429991274	30.579839566825278	19.766427415183248
14	22.216674859035496	27.335404158292008	28.992235693164474	21.45568528950802
15	21.648184511018524	25.063244229527015	31.905459205863508	21.38311205359096
16	23.398177433841262	24.321041348729842	30.130245796800086	22.150535420628813
17	24.01324847583337	24.14218177794134	27.93889436476544	23.905675381459854
18	24.44662907613241	24.19802714033142	29.36024891075808	21.995094872778086
19	25.049347226904594	25.869785085427964	27.3336026949891	21.74726499267834
20	25.388794383552128	25.385706160747144	28.167422852334052	21.058076603366676
21	25.139935095850714	26.4308122283329	27.61668978544572	20.812562890370664
22	23.27928085584948	24.921443332398287	27.23580897283136	24.56346683892087
23	23.240163366986383	25.91688048320393	28.190069819570585	22.652886330239106
24	23.00056874769992	26.226217467502888	27.92087973173638	22.852334053060815
25	23.232700161874345	26.508789854158678	26.662686290606914	23.595823693360064
26	22.522408916728644	26.857759031121564	27.23297810192679	23.386853950222996
27	24.1210789221073	25.84636606249019	27.088346333893504	22.94420868150901
28	21.855352790852685	26.07257838295507	28.62550923507295	23.4465595911193
29	23.97696185787484	25.88779971845702	26.884780980665152	23.25045744300299
30	24.637069482439593	26.269967290573458	26.512650132664906	22.580313094322044
31	25.46008085996711	26.553054381030076	24.397989566953957	23.588875192048857
32	24.804605569609826	27.61257215503908	24.415746848082602	23.167075427268493
33	24.833428982456322	26.118901725029787	25.538573189593716	23.50909610292017
34	23.361633463982315	26.48717229452381	25.94518919224959	24.206005049244286
35	24.744899928713522	25.329860798357068	26.056879917029747	23.868359355899663
36	25.809564740730828	26.573385181162873	25.69246962604195	21.924580452064347
37	23.02784804914392	27.949960496483286	26.001034554639666	23.021156899733125
38	22.756341794205976	28.41164980582799	24.928391833709497	23.903616566256535
39	23.950969315932912	26.13408548715428	26.64956134368574	23.265383853227064
40	24.29736497389165	25.788204532996374	27.26797796038325	22.646452532728727
41	22.265057016313534	26.259930566457268	26.600921834507286	24.87409058272191
42	22.468622369541887	25.88368208805038	27.26617649708034	24.38151904532739
43	23.46817715075417	24.588172621360723	27.634447066574364	24.309203161310744
44	23.34670705375824	25.20375836715366	27.032500971503424	24.417033607584678
45	23.880197543318758	25.39677229246499	26.117614965527714	24.605415198688537
46	24.500158271418755	25.71280042617475	26.422576967519618	23.364464334886883
47	22.738069809276507	24.955671135153498	28.7541851852805	23.552073870289494
48	22.011050690603824	25.276588954971135	27.886909280881582	24.82545107354345
49	22.757885905608468	24.659459097775706	29.439513296085934	23.14314170052989
50	22.580827798122876	24.208835920148854	28.80900114006892	24.401335141659352
51	22.65468779354201	24.204460937841795	26.8788618869556	26.261989381660587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	441.5
2	690.0
3	1817.0
4	2944.0
5	1977.5
6	1011.0
7	994.5
8	978.0
9	1000.0
10	1022.0
11	1007.0
12	992.0
13	1000.5
14	1009.0
15	986.5
16	964.0
17	899.5
18	835.0
19	961.0
20	1087.0
21	1159.0
22	1231.0
23	1301.5
24	1372.0
25	1964.5
26	3039.0
27	3521.0
28	4000.5
29	4480.0
30	5313.0
31	6146.0
32	6829.0
33	7512.0
34	7946.0
35	8380.0
36	9215.5
37	10051.0
38	10640.0
39	11229.0
40	13459.0
41	15689.0
42	17009.0
43	18329.0
44	19596.0
45	20863.0
46	23246.5
47	25630.0
48	29376.0
49	33122.0
50	34582.0
51	36042.0
52	35140.5
53	34239.0
54	30698.5
55	27158.0
56	24822.0
57	22486.0
58	21134.0
59	19782.0
60	18280.0
61	16778.0
62	15750.5
63	14723.0
64	12663.0
65	10603.0
66	9145.0
67	7687.0
68	6837.5
69	5988.0
70	5128.5
71	4269.0
72	3490.5
73	2712.0
74	2305.0
75	1615.5
76	1333.0
77	916.0
78	499.0
79	382.0
80	265.0
81	237.0
82	209.0
83	128.0
84	47.0
85	25.0
86	3.0
87	8.0
88	13.0
89	7.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	388573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.06148953241712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04629337645764	23.819577743152067
2	8.43126757177874	5.5750052913874155
3	3.3327325765279565	3.3055530957967365
4	2.0676969382173125	2.734445627163304
5	1.4971565705202647	2.474911314232262
6	1.2900575502849243	2.559073451697642
7	1.1003653962694653	2.546580332141786
8	1.024427512653925	2.7095279589062278
9	0.924372491625957	2.750501831035132
>10	8.171550305008605	45.87407224332136
>50	0.07110447718920669	1.6272323877313273
>100	0.039068394060003675	2.8158172767536858
>500	0.0023441036436002206	0.42908921987962134
>1k	0.0015627357624001471	0.7786122268014322
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1837	0.4727554410625545	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1177	0.30290318678858286	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	567	0.14591852753536658	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	552	0.14205824902913997	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	542	0.13948473002498885	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	487	0.1253303755021579	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC	471	0.12121274509551616	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	430	0.11066131717849671	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	407	0.10474222346894921	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	402	0.10345546396687366	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	397	0.10216870446479812	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	395	0.1016540006639679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5735190041510864E-4	0.0	0.0	0.14900675034034788	0.0
2	2.5735190041510864E-4	0.0	0.0	0.7041147995357372	0.0
3	2.5735190041510864E-4	0.0	0.0	0.9537461429383925	0.0
4	2.5735190041510864E-4	0.0	0.0	1.4017958015610967	0.0
5	2.5735190041510864E-4	0.0	0.0	2.4458724615451923	0.0
6	2.5735190041510864E-4	0.0	0.0	3.023112774176281	0.0
7	2.5735190041510864E-4	0.0	0.0	3.5952060487990676	0.0
8	2.5735190041510864E-4	0.0	0.0	4.5263052245009305	0.0
9	2.5735190041510864E-4	0.0	0.0	4.912333075123593	0.0
10	2.5735190041510864E-4	0.0	0.0	5.865564514261155	0.0
11	2.5735190041510864E-4	0.0	0.0	6.966773296137405	0.0
12	2.5735190041510864E-4	0.0	0.0	7.929784107490741	0.0
13	2.5735190041510864E-4	0.0	0.0	8.309378160603027	0.0
14	2.5735190041510864E-4	0.0	0.0	8.453495224835487	0.0
15	2.5735190041510864E-4	0.0	0.0	8.749192558412448	0.0
16	2.5735190041510864E-4	0.0	0.0	9.34521955977384	0.0
17	2.5735190041510864E-4	0.0	0.0	9.995290460222403	0.0
18	2.5735190041510864E-4	0.0	0.0	10.739037452422068	0.0
19	2.5735190041510864E-4	0.0	0.0	11.138962305667146	0.0
20	2.5735190041510864E-4	0.0	0.0	11.54815182732717	0.0
21	2.5735190041510864E-4	0.0	0.0	12.02373813929429	0.0
22	2.5735190041510864E-4	0.0	0.0	12.517853788091298	0.0
23	2.5735190041510864E-4	0.0	0.0	13.034616404124836	0.0
24	2.5735190041510864E-4	0.0	0.0	13.464394077818067	0.0
25	5.147038008302173E-4	0.0	0.0	13.83935579672288	0.0
26	5.147038008302173E-4	0.0	0.0	14.185236750880787	0.0
27	5.147038008302173E-4	0.0	0.0	14.534205927843674	0.0
28	5.147038008302173E-4	0.0	0.0	14.9042779606406	0.0
29	5.147038008302173E-4	0.0	0.0	15.266372084524658	0.0
30	5.147038008302173E-4	0.0	0.0	15.669899864375548	0.0
31	5.147038008302173E-4	0.0	0.0	16.07857468223474	0.0
32	5.147038008302173E-4	0.0	0.0	16.467176051861554	0.0
33	5.147038008302173E-4	0.0	0.0	16.869159720309955	0.0
34	5.147038008302173E-4	0.0	0.0	17.265224295048807	0.0
35	5.147038008302173E-4	0.0	0.0	17.660259462185998	0.0
36	5.147038008302173E-4	0.0	0.0	18.035993236792056	0.0
37	5.147038008302173E-4	0.0	0.0	18.40040352777985	0.0
38	7.720557012453258E-4	0.0	0.0	18.77665200618674	0.0
39	7.720557012453258E-4	0.0	0.0	19.16551072771397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAGAG	30	2.161778E-6	45.000004	1
CGGAATC	20	7.027516E-4	45.0	19
GACCGTT	35	1.209155E-7	45.0	1
CGACAAT	25	3.8860235E-5	45.0	20
CGCTAGG	20	7.027516E-4	45.0	2
CGAATAT	40	6.7957444E-9	45.0	14
TTAACGG	25	3.8860235E-5	45.0	2
TCATCGA	25	3.8860235E-5	45.0	16
TTCATCG	25	3.8860235E-5	45.0	15
CCGTTAG	25	3.8860235E-5	45.0	1
GCACTAG	20	7.027516E-4	45.0	1
CCTCGTG	65	0.0	44.999996	15
CGAGGGT	60	3.6379788E-12	41.250004	4
CATACGA	55	6.002665E-11	40.909092	18
ACATACG	55	6.002665E-11	40.909092	17
CGAGATA	95	0.0	40.263157	19
CGTATGG	45	1.9226718E-8	40.0	2
ACGTAGG	45	1.9226718E-8	40.0	2
ACGGGCT	120	0.0	39.375004	5
GCGATAC	40	3.45035E-7	39.375	9
>>END_MODULE