FastQCFastQC Report
Sat 18 Jun 2016
SRR3551464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458625
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC49561.0806214227309894No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG49011.0686290542382122No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC43950.9582992641046607No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35190.7672935404742436No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC21240.46312346688470973TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT9920.21629871899700193No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT9420.20539656582174978TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT9020.19667484328154808No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC8830.19253202507495232No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT8820.19231398201144725No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC8810.19209593894794222TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC7360.1604796947397111No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA6270.13671300081766147No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC4780.1042245843554102TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGG207.028847E-445.0000042
AGCGCGC207.028847E-445.00000445
CGCGTAA406.7993824E-945.00000431
TCGCGTA406.7993824E-945.00000430
CGTATGG351.2097917E-745.02
TTGCGAC253.8871265E-545.01
GCGTATG253.8871265E-545.01
CGTTTTT17250.041.739131
CGCATGG603.6379788E-1241.2499962
CTCGAAT603.6379788E-1241.24999643
TATGCGG501.0786607E-940.52
CGTAACT501.0786607E-940.533
ACACGAC451.9239451E-840.026
GCCGACC451.9239451E-840.014
AGTACGG850.039.7058832
TACGGCT4450.039.43827
CTACGAA403.452169E-739.37500411
ATGGGCG750.039.05
GCCCTAC750.039.026
TCACGAC356.2400795E-638.57142625