##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458625
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.214351594439904	33.0	31.0	34.0	30.0	34.0
2	32.3006094303625	34.0	31.0	34.0	30.0	34.0
3	32.306319978195695	34.0	31.0	34.0	30.0	34.0
4	35.886645952575634	37.0	35.0	37.0	35.0	37.0
5	35.934992641046605	37.0	35.0	37.0	35.0	37.0
6	35.92115780866721	37.0	35.0	37.0	35.0	37.0
7	36.094918506405016	37.0	35.0	37.0	35.0	37.0
8	35.87850858544562	37.0	35.0	37.0	35.0	37.0
9	37.774813845734535	39.0	38.0	39.0	35.0	39.0
10	37.44479912782774	39.0	37.0	39.0	34.0	39.0
11	37.47500245298446	39.0	37.0	39.0	35.0	39.0
12	37.4012319433088	39.0	37.0	39.0	35.0	39.0
13	37.382423548650856	39.0	37.0	39.0	35.0	39.0
14	38.66200490596893	40.0	38.0	41.0	35.0	41.0
15	38.67021422730989	40.0	38.0	41.0	35.0	41.0
16	38.70866176069774	40.0	38.0	41.0	35.0	41.0
17	38.61040283455983	40.0	38.0	41.0	34.0	41.0
18	38.525784682474786	40.0	38.0	41.0	34.0	41.0
19	38.43511583537749	40.0	37.0	41.0	34.0	41.0
20	38.281620059961845	40.0	37.0	41.0	34.0	41.0
21	38.24239629326792	40.0	37.0	41.0	34.0	41.0
22	38.23973180703189	40.0	37.0	41.0	34.0	41.0
23	38.1891196511311	40.0	37.0	41.0	34.0	41.0
24	38.14527773235214	40.0	37.0	41.0	34.0	41.0
25	38.010771327337146	40.0	36.0	41.0	34.0	41.0
26	38.027948759880076	40.0	37.0	41.0	34.0	41.0
27	37.973095666394116	40.0	36.0	41.0	34.0	41.0
28	37.89532624693377	40.0	36.0	41.0	34.0	41.0
29	37.83392968111202	40.0	36.0	41.0	33.0	41.0
30	37.644456800218045	40.0	36.0	41.0	33.0	41.0
31	37.64225892613791	40.0	36.0	41.0	33.0	41.0
32	37.43281984191878	40.0	36.0	41.0	33.0	41.0
33	37.254600163532295	40.0	36.0	41.0	32.0	41.0
34	37.166517307168164	40.0	36.0	41.0	32.0	41.0
35	37.01199890978468	40.0	35.0	41.0	31.0	41.0
36	36.94047206323249	40.0	35.0	41.0	31.0	41.0
37	36.87934805124012	40.0	35.0	41.0	31.0	41.0
38	36.81704442627419	40.0	35.0	41.0	31.0	41.0
39	36.77093485963478	40.0	35.0	41.0	31.0	41.0
40	36.67260397928591	39.0	35.0	41.0	31.0	41.0
41	36.62584246388662	39.0	35.0	41.0	31.0	41.0
42	36.533165440174436	39.0	35.0	41.0	31.0	41.0
43	36.4171076587626	39.0	35.0	41.0	30.0	41.0
44	36.47204796947397	39.0	35.0	41.0	31.0	41.0
45	36.43611665303897	39.0	35.0	41.0	31.0	41.0
46	36.359474516216956	39.0	35.0	41.0	30.0	41.0
47	36.253291905151265	39.0	35.0	41.0	30.0	41.0
48	36.22736222403925	39.0	35.0	41.0	30.0	41.0
49	36.20772308530935	39.0	35.0	41.0	30.0	41.0
50	36.09159334968656	39.0	35.0	41.0	30.0	41.0
51	35.29117688743527	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	3.0
11	8.0
12	4.0
13	15.0
14	21.0
15	12.0
16	26.0
17	61.0
18	121.0
19	233.0
20	452.0
21	903.0
22	1476.0
23	1830.0
24	2460.0
25	3283.0
26	4239.0
27	4876.0
28	5135.0
29	5325.0
30	5932.0
31	7282.0
32	9432.0
33	12804.0
34	23469.0
35	33181.0
36	32009.0
37	47769.0
38	86890.0
39	169164.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.090160806759336	19.188007631507222	21.70902153175252	13.01281002998092
2	33.08411011174707	27.83952030526029	22.219460343417825	16.856909239574815
3	30.861161079313167	27.20610520577814	25.594112837285365	16.33862087762333
4	27.649822840010902	23.683619514854186	30.5125102207686	18.15404742436631
5	27.138293813028074	27.931316434995914	24.878059416734803	20.052330335241212
6	24.013736713000817	36.672662850913056	25.09021531752521	14.223385118560916
7	73.76723902970836	6.24911420005451	14.986753883892067	4.996892886345053
8	72.68879803761243	9.638375579176888	11.890542382120469	5.782284001090216
9	66.92461161079312	7.791550831289179	13.486181520850367	11.797656037067322
10	37.72886345053148	22.959498500953938	22.44884164622513	16.86279640228945
11	31.084001090215317	22.54085581902426	25.343799400381577	21.03134369037885
12	25.249822840010903	20.544453529572092	31.611665303897517	22.594058326519487
13	24.79498500953938	21.796892886345056	33.952357590624146	19.455764513491413
14	21.148214772417553	27.17296266012538	29.729299536658488	21.94952303079858
15	19.553447805941673	24.07435268465522	35.04039247751431	21.331807031888797
16	21.05205778141183	23.319051512673752	30.594930498773508	25.03396020714091
17	21.125320250749525	23.927609702916325	30.80163532297629	24.145434723357866
18	21.835704551648952	23.916707549741073	30.829108748977923	23.418479149632052
19	22.513818479149634	25.004960479694738	28.453529572090485	24.02769146906514
20	24.451349141455438	25.011937857726902	30.386917416189696	20.149795584627963
21	23.903406922867266	26.896920141727993	29.075606432270373	20.12406650313437
22	21.84769692014173	23.38075769964568	28.756827473426	26.01471790678659
23	22.446007086399565	26.648133006268736	28.651512673753064	22.25434723357863
24	23.102534750613245	24.7912782774598	27.813791223766692	24.29239574816026
25	21.152575633687654	28.722594712455713	26.9363859362224	23.188443717634232
26	21.34663396020714	24.650640501499048	29.838539111474518	24.1641864268193
27	23.78675388389207	25.0206595802671	28.272117743254295	22.920468792586533
28	20.624257290814935	25.305205778141183	30.495938947942218	23.574597983101665
29	24.164404469882804	24.549468520032704	28.007849550286178	23.27827745979831
30	21.965222131370947	26.590351594439905	29.7340964840556	21.71032979013355
31	23.17710547833197	24.939111474516217	25.704660670482422	26.179122376669394
32	24.344289997274462	27.61689833742164	25.42316707549741	22.615644589806486
33	22.332842736440448	26.432270373398747	25.872117743254293	25.362769146906516
34	23.130226219678388	24.23614063777596	28.02965385663669	24.603979285908967
35	22.88165712728264	23.96642136822022	26.95557372581085	26.19634777868629
36	22.492014172799127	25.746742981738898	26.345489234123736	25.41575361133824
37	20.41864268192968	26.788116653038973	28.975960752248568	23.817279912782773
38	19.802234941400926	28.083946579449442	25.070373398746256	27.043445080403377
39	22.61499046061597	26.040883074407194	27.849332243118013	23.494794221858818
40	21.6691196511311	24.540964840556008	29.214717906786593	24.575197601526302
41	19.89010629599346	24.971599890978467	27.968165712728265	27.170128100299813
42	21.009539384028344	23.95857181793404	28.882202234941403	26.149686563096214
43	22.896266012537474	23.55584627964023	27.196293267920414	26.35159443990188
44	21.89065140365222	24.086563096211503	28.345162169528482	25.677623330607798
45	21.634886890160807	23.229217770509674	27.94156445898065	27.194330880348872
46	24.088525483783048	25.205560098119378	27.26105205778141	23.44486236031616
47	20.42256745707277	24.573235213954757	31.54843281548106	23.455764513491413
48	21.22060506950123	24.23962932679204	28.434559825565547	26.105205778141183
49	21.09195966203325	22.7632597437994	31.008994276369584	25.135786317797766
50	21.025674570727716	22.365549195966203	30.05113109839193	26.557645134914143
51	21.142109566639412	22.356173344235486	27.882474788770782	28.61924230035432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	541.0
2	837.0
3	3230.5
4	5624.0
5	3687.0
6	1750.0
7	1711.0
8	1672.0
9	1679.5
10	1687.0
11	1641.0
12	1595.0
13	1577.5
14	1560.0
15	1490.5
16	1421.0
17	1361.5
18	1302.0
19	1322.0
20	1342.0
21	1374.5
22	1407.0
23	1547.0
24	1687.0
25	2072.0
26	2961.0
27	3465.0
28	3999.5
29	4534.0
30	5388.0
31	6242.0
32	6818.5
33	7395.0
34	7854.0
35	8313.0
36	9498.5
37	10684.0
38	11519.5
39	12355.0
40	14469.5
41	16584.0
42	18426.0
43	20268.0
44	23454.0
45	26640.0
46	39133.0
47	51626.0
48	47886.0
49	44146.0
50	44023.0
51	43900.0
52	38573.0
53	33246.0
54	30393.5
55	27541.0
56	25324.5
57	23108.0
58	21690.5
59	20273.0
60	19353.0
61	18433.0
62	17363.0
63	16293.0
64	14204.0
65	12115.0
66	9993.0
67	7871.0
68	7112.0
69	6353.0
70	5414.0
71	4475.0
72	3948.0
73	3421.0
74	2821.0
75	1674.0
76	1127.0
77	942.5
78	758.0
79	571.5
80	385.0
81	288.5
82	192.0
83	124.0
84	56.0
85	32.5
86	9.0
87	6.5
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.48133764900992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6214940892413	25.040862582667074
2	9.113528846042122	6.284933306287405
3	3.405525261984414	3.522811992921527
4	1.9976651548165434	2.755286668515637
5	1.5446759362674682	2.6631246258369554
6	1.3110545295318674	2.7124148345431305
7	1.1307996098042177	2.7294038211319536
8	0.956063044959974	2.6373066133604333
9	0.8449706810505273	2.6222147421135396
>10	6.961259766767449	39.138697351481625
>50	0.06155853210570834	1.443819614603429
>100	0.04315443487822853	2.5976936412484553
>500	0.005076992338615121	1.4978698487360522
>1k	0.0031731202116344506	4.3535603565527765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	4956	1.0806214227309894	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	4901	1.0686290542382122	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	4395	0.9582992641046607	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3519	0.7672935404742436	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2124	0.46312346688470973	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	992	0.21629871899700193	No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	942	0.20539656582174978	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT	902	0.19667484328154808	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC	883	0.19253202507495232	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	882	0.19231398201144725	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	881	0.19209593894794222	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC	736	0.1604796947397111	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA	627	0.13671300081766147	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	478	0.1042245843554102	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1804306350504225E-4	0.0	0.0	0.21150177159989098	0.0
2	2.1804306350504225E-4	0.0	0.0	1.069065140365222	0.0
3	2.1804306350504225E-4	0.0	0.0	1.4063777596075224	0.0
4	2.1804306350504225E-4	0.0	0.0	2.0007631507222676	0.0
5	2.1804306350504225E-4	0.0	0.0	4.2075769964568	0.0
6	2.1804306350504225E-4	0.0	0.0	5.0047424366312345	0.0
7	2.1804306350504225E-4	0.0	0.0	5.900681384573454	0.0
8	2.1804306350504225E-4	0.0	0.0	7.1014445352957205	0.0
9	2.1804306350504225E-4	0.0	0.0	7.575906241482693	0.0
10	2.1804306350504225E-4	0.0	0.0	9.69811937857727	0.0
11	2.1804306350504225E-4	0.0	0.0	11.112346688470973	0.0
12	2.1804306350504225E-4	0.0	0.0	13.153011719814664	0.0
13	2.1804306350504225E-4	0.0	0.0	13.680457890433361	0.0
14	2.1804306350504225E-4	0.0	0.0	13.897628781684382	0.0
15	2.1804306350504225E-4	0.0	0.0	14.384736985554648	0.0
16	2.1804306350504225E-4	0.0	0.0	15.08923412373944	0.0
17	2.1804306350504225E-4	0.0	0.0	15.902970836740256	0.0
18	2.1804306350504225E-4	0.0	0.0	16.802398473698556	2.1804306350504225E-4
19	2.1804306350504225E-4	0.0	0.0	17.521940583265195	2.1804306350504225E-4
20	2.1804306350504225E-4	0.0	0.0	18.0411011174707	2.1804306350504225E-4
21	2.1804306350504225E-4	0.0	0.0	18.60844916871082	2.1804306350504225E-4
22	4.360861270100845E-4	0.0	0.0	19.24709730171709	2.1804306350504225E-4
23	4.360861270100845E-4	0.0	0.0	19.855437448896158	2.1804306350504225E-4
24	4.360861270100845E-4	0.0	0.0	20.323139820114474	2.1804306350504225E-4
25	4.360861270100845E-4	0.0	0.0	20.742436631234668	2.1804306350504225E-4
26	4.360861270100845E-4	0.0	0.0	21.13774870536931	2.1804306350504225E-4
27	4.360861270100845E-4	0.0	0.0	21.559225947124556	2.1804306350504225E-4
28	4.360861270100845E-4	0.0	0.0	21.955628236576725	2.1804306350504225E-4
29	4.360861270100845E-4	0.0	0.0	22.364240937585173	2.1804306350504225E-4
30	4.360861270100845E-4	0.0	0.0	22.814499863723086	2.1804306350504225E-4
31	4.360861270100845E-4	0.0	0.0	23.24971381847915	2.1804306350504225E-4
32	4.360861270100845E-4	0.0	0.0	23.669446715726355	2.1804306350504225E-4
33	4.360861270100845E-4	0.0	0.0	24.10291632597438	2.1804306350504225E-4
34	4.360861270100845E-4	0.0	0.0	24.505205778141182	2.1804306350504225E-4
35	4.360861270100845E-4	0.0	0.0	24.9340964840556	2.1804306350504225E-4
36	4.360861270100845E-4	0.0	0.0	25.337912237666938	2.1804306350504225E-4
37	4.360861270100845E-4	0.0	0.0	25.72711910602344	2.1804306350504225E-4
38	4.360861270100845E-4	0.0	0.0	26.12417552466612	2.1804306350504225E-4
39	4.360861270100845E-4	0.0	0.0	26.51730716816571	2.1804306350504225E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGG	20	7.028847E-4	45.000004	2
AGCGCGC	20	7.028847E-4	45.000004	45
CGCGTAA	40	6.7993824E-9	45.000004	31
TCGCGTA	40	6.7993824E-9	45.000004	30
CGTATGG	35	1.2097917E-7	45.0	2
TTGCGAC	25	3.8871265E-5	45.0	1
GCGTATG	25	3.8871265E-5	45.0	1
CGTTTTT	1725	0.0	41.73913	1
CGCATGG	60	3.6379788E-12	41.249996	2
CTCGAAT	60	3.6379788E-12	41.249996	43
TATGCGG	50	1.0786607E-9	40.5	2
CGTAACT	50	1.0786607E-9	40.5	33
ACACGAC	45	1.9239451E-8	40.0	26
GCCGACC	45	1.9239451E-8	40.0	14
AGTACGG	85	0.0	39.705883	2
TACGGCT	445	0.0	39.4382	7
CTACGAA	40	3.452169E-7	39.375004	11
ATGGGCG	75	0.0	39.0	5
GCCCTAC	75	0.0	39.0	26
TCACGAC	35	6.2400795E-6	38.571426	25
>>END_MODULE