SRR3551461_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551461_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522157
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20157	3.860333194805394	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	6846	1.3110999182238292	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	4133	0.7915243882587039	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	3606	0.6905968894412983	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1994	0.38187748129394034	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1398	0.2677355661228328	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	1294	0.2478181849520355	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	1093	0.20932401557385996	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	1034	0.19802473202504225	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	957	0.18327820942743275	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	954	0.18270366958596743	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	866	0.16585050090298512	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	811	0.15531727047612118	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	793	0.15187003142732933	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	773	0.14803976581756062	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	655	0.12544119871992523	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	646	0.12371757919552931	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	641	0.12276001279308713	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	606	0.1160570479759919	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	590	0.11299283548817693	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAC	30	2.1632168E-6	45.000004	32
TATTGCG	20	7.029746E-4	45.0	1
ACGATAG	25	3.8878712E-5	45.0	1
CGTTTTT	8915	0.0	44.268085	1
TACGAAT	65	0.0	41.538464	12
GCTACGA	65	0.0	41.538464	10
CCTATGC	65	0.0	41.538464	35
ATTGCGG	50	1.0786607E-9	40.5	2
CACGACC	45	1.9248546E-8	40.0	27
ACGACCA	45	1.9248546E-8	40.0	28
CGGGCAC	40	3.4533878E-7	39.375	6
GCGATAC	40	3.4533878E-7	39.375	9
GTTTTTT	10115	0.0	39.238754	2
ACCCGTC	35	6.2417403E-6	38.571426	39
CACGTGA	35	6.2417403E-6	38.571426	43
CGCGTAA	35	6.2417403E-6	38.571426	31
AACAGCG	35	6.2417403E-6	38.571426	1
TCGCGTA	35	6.2417403E-6	38.571426	30
AACACGT	35	6.2417403E-6	38.571426	41
AACGGGC	65	9.094947E-12	38.076927	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content