##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551461_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522157
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.212821048075575	33.0	31.0	34.0	30.0	34.0
2	32.35405251677177	34.0	31.0	34.0	30.0	34.0
3	32.250625769643996	33.0	31.0	34.0	30.0	34.0
4	35.89453555156782	37.0	35.0	37.0	35.0	37.0
5	35.95859865902401	37.0	35.0	37.0	35.0	37.0
6	35.924945945376585	37.0	35.0	37.0	35.0	37.0
7	36.10368529005644	37.0	35.0	37.0	35.0	37.0
8	35.903119176799315	37.0	35.0	37.0	35.0	37.0
9	37.82570567856028	39.0	38.0	39.0	35.0	39.0
10	37.56787900956992	39.0	37.0	39.0	35.0	39.0
11	37.56209339336636	39.0	37.0	39.0	35.0	39.0
12	37.49537974210822	39.0	37.0	39.0	35.0	39.0
13	37.48578684188855	39.0	37.0	39.0	35.0	39.0
14	38.80541867675814	40.0	38.0	41.0	35.0	41.0
15	38.80326989775106	40.0	38.0	41.0	35.0	41.0
16	38.81751274042099	40.0	38.0	41.0	35.0	41.0
17	38.72187100814506	40.0	38.0	41.0	34.0	41.0
18	38.388982624000064	39.0	38.0	41.0	34.0	41.0
19	38.06278954414094	39.0	37.0	41.0	34.0	41.0
20	37.68081822133956	39.0	35.0	41.0	34.0	41.0
21	37.63122011195866	39.0	35.0	41.0	34.0	41.0
22	37.643731674572976	39.0	35.0	41.0	34.0	41.0
23	37.58764126498352	39.0	35.0	41.0	34.0	41.0
24	37.50319156881934	39.0	35.0	41.0	33.0	41.0
25	37.37169855043598	39.0	35.0	41.0	33.0	41.0
26	37.363902044787295	39.0	35.0	41.0	33.0	41.0
27	37.334110238874516	39.0	35.0	41.0	33.0	41.0
28	37.212560590014114	39.0	35.0	41.0	33.0	41.0
29	37.081163328270996	39.0	35.0	41.0	33.0	41.0
30	36.81483729989256	39.0	35.0	41.0	32.0	41.0
31	36.58216973055997	39.0	35.0	41.0	31.0	41.0
32	36.077495848949646	39.0	35.0	41.0	30.0	41.0
33	35.522540155547084	39.0	35.0	41.0	24.0	41.0
34	34.99762332018914	39.0	35.0	41.0	21.0	41.0
35	34.67402716041344	39.0	35.0	41.0	18.0	41.0
36	34.49298774123491	39.0	35.0	41.0	17.0	41.0
37	34.41349057850417	39.0	35.0	41.0	15.0	41.0
38	34.28983428355839	39.0	35.0	41.0	15.0	41.0
39	34.198099422204436	38.0	34.0	41.0	15.0	41.0
40	34.08944053225371	38.0	34.0	41.0	15.0	41.0
41	33.96090639405389	38.0	34.0	41.0	15.0	41.0
42	33.826146159105406	38.0	34.0	41.0	13.0	41.0
43	33.709912918911364	38.0	34.0	41.0	12.0	41.0
44	33.746924775498556	38.0	34.0	40.0	13.0	41.0
45	33.741943515073054	38.0	34.0	40.0	13.0	41.0
46	33.683392159829324	38.0	33.0	40.0	14.0	41.0
47	33.603393232303695	37.0	33.0	40.0	15.0	41.0
48	33.58448321098827	37.0	33.0	40.0	15.0	41.0
49	33.58533736021924	37.0	33.0	40.0	15.0	41.0
50	33.46344873285238	37.0	33.0	40.0	14.0	41.0
51	32.71453030410394	36.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	8.0
11	10.0
12	7.0
13	13.0
14	16.0
15	40.0
16	41.0
17	104.0
18	210.0
19	445.0
20	903.0
21	1332.0
22	2112.0
23	3149.0
24	4846.0
25	8584.0
26	13480.0
27	16217.0
28	14569.0
29	12008.0
30	10763.0
31	10677.0
32	12144.0
33	15503.0
34	25975.0
35	36535.0
36	39541.0
37	48292.0
38	85026.0
39	159435.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.40112073571742	16.632162357298665	19.971579429175517	26.995137477808402
2	43.4396168202284	24.209576813104107	18.769833594110583	13.580972772556912
3	25.11734976261929	23.69612970811461	37.22577692150062	13.96074360776548
4	22.480020377013044	19.992454376748757	41.756981137857004	15.770544108381197
5	23.563219491455634	22.97355010083174	36.10887146969207	17.354358938020557
6	20.88452323726389	29.974509582366988	36.27625407683896	12.864713103530164
7	63.28690413036691	4.97838772629688	27.400954119163394	4.333754024172806
8	63.175443401122656	7.4810832757197545	24.531510637605162	4.811962685552429
9	57.56659395545785	6.327215760776932	25.902362699341385	10.203827584423841
10	32.701467183241824	19.688139774052633	33.05404313262103	14.556349910084515
11	26.018994287158844	19.12738888878249	36.709074090742824	18.14454273331584
12	21.16604775958189	17.114201284288058	41.247938838318746	20.471812117811307
13	21.19362567197222	17.70425370147293	44.06912097319389	17.03299965336096
14	18.151820237974402	22.42563060535433	40.49835585848701	18.924193298184264
15	17.403960877667064	20.230122357834905	44.01607179449859	18.349844969999445
16	19.308177425563574	20.510306287189486	39.92362450374121	20.257891783505727
17	19.128729481745914	21.723542919083723	39.33644478576367	19.8112828134067
18	19.222762502465734	21.11702035977685	39.15489019586063	20.505326941896787
19	18.79492183385457	23.179235364076323	37.49542762042834	20.53041518164077
20	20.335454662103544	22.61024940774518	38.84540473459132	18.208891195559957
21	19.824688743040888	24.16610329843323	38.54530342406594	17.463904534459942
22	18.553232073878164	20.5415229519091	39.41592279716637	21.489322177046365
23	19.392826295539464	22.604120982769548	38.64814605568823	19.354906666002755
24	20.105447212236932	21.80608514297424	37.707815848490014	20.380651796298814
25	19.49279622795443	24.76802953900838	36.136449382082404	19.60272485095479
26	18.310201720938338	22.042029504535993	38.25267113147961	21.395097643046057
27	21.148237024496463	21.242844585057753	36.88430874238974	20.724609648056045
28	18.726743106000683	22.367602081366332	38.387304967662985	20.51834984497
29	19.866247124906877	22.082247293438563	37.26484563072026	20.786659950934297
30	19.316604009905067	22.99308445544156	37.33225830545219	20.35805322920118
31	20.300790758335136	21.42516522808274	36.32470693680253	21.94933707677959
32	20.657196973324115	23.09765070658825	36.69337000174277	19.551782318344866
33	20.93450820347137	21.719521140193468	35.70994930643466	21.63602134990051
34	21.547925240875827	23.145529026710356	34.72480499160214	20.58174074081167
35	21.122765758191502	22.49993775818384	33.11264619645049	23.264650287174163
36	21.863347613840283	24.849805709776945	32.70280777620524	20.584038900177532
37	21.458680052168216	23.29701603157671	34.047614031795035	21.19668988446004
38	20.763869870556174	23.533534932979926	33.30186131757307	22.400733878890833
39	23.614353537346048	22.889667283977808	32.76447505252252	20.731504126153627
40	21.373265129070376	22.4095434897933	34.46970930199155	21.747482079144778
41	21.89169157935257	24.216662804482176	31.64795262727494	22.243692988890317
42	21.108785288715843	24.095434897932996	32.85735899355941	21.938420819791748
43	22.566009839952354	23.151082911844522	32.01872233830055	22.264184909902575
44	21.7670164337546	22.86247239814845	32.814460018729996	22.556051149366954
45	21.32251410974094	21.857027675584163	33.049829840450286	23.77062837422461
46	22.78778221875796	23.620864988882655	32.40232343911889	21.189029353240503
47	19.86701317802883	23.116227494795627	36.09680613302129	20.919953194154246
48	20.396355885298867	22.786250112514054	33.91987467370925	22.897519328477834
49	20.265552314725266	21.603464092217475	35.84036985044728	22.29061374260998
50	19.941320330858343	21.189029353240503	36.077654804972454	22.791995510928707
51	19.749040997247956	21.5467761611929	34.256555020807916	24.44762782075123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	282.0
1	577.0
2	872.0
3	15316.5
4	29761.0
5	18845.0
6	7929.0
7	7827.0
8	7725.0
9	7644.0
10	7563.0
11	7470.0
12	7377.0
13	7099.0
14	6821.0
15	6406.0
16	5991.0
17	5522.5
18	5054.0
19	4747.5
20	4441.0
21	4137.0
22	3833.0
23	3674.5
24	3516.0
25	3759.5
26	4307.5
27	4612.0
28	4793.0
29	4974.0
30	5552.5
31	6131.0
32	6828.5
33	7526.0
34	8262.5
35	8999.0
36	9870.0
37	10741.0
38	11612.0
39	12483.0
40	14188.5
41	15894.0
42	17374.5
43	18855.0
44	21092.0
45	23329.0
46	38264.5
47	53200.0
48	47547.0
49	41894.0
50	41176.5
51	40459.0
52	35803.5
53	31148.0
54	28633.5
55	26119.0
56	24848.5
57	23578.0
58	21909.5
59	20241.0
60	19321.0
61	18401.0
62	17393.0
63	16385.0
64	14741.5
65	13098.0
66	11227.0
67	9356.0
68	7807.5
69	6259.0
70	5503.0
71	4747.0
72	4078.5
73	3410.0
74	2853.5
75	1829.0
76	1361.0
77	1096.5
78	832.0
79	599.5
80	367.0
81	262.5
82	158.0
83	117.0
84	76.0
85	58.5
86	41.0
87	27.0
88	13.0
89	7.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	522157.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.567248338629255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20660573656451	31.89410372153027
2	9.00696600196222	7.848174491701576
3	4.623351173079156	6.042800661426974
4	3.1232835520058515	5.4429148056878
5	2.305701835599617	5.022654223320091
6	1.7749457203546766	4.639770059176763
7	1.4166924009294322	4.320497275551904
8	1.1418761616790913	3.9798721846266996
9	0.8174294123525395	3.2051835186536577
>10	2.519023358901704	15.124128479493997
>50	0.03184120381758411	0.9577692215169764
>100	0.022996424975185042	1.817468731706908
>500	0.005306867301965779	1.69820517346175
>1k	0.003095672592813371	2.8037533111204205
>5k	4.4223894183048157E-4	1.3190279802041232
>10k+	4.4223894183048157E-4	3.8836761608201154
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20157	3.860333194805394	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	6846	1.3110999182238292	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	4133	0.7915243882587039	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	3606	0.6905968894412983	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1994	0.38187748129394034	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1398	0.2677355661228328	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	1294	0.2478181849520355	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	1093	0.20932401557385996	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	1034	0.19802473202504225	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	957	0.18327820942743275	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	954	0.18270366958596743	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	866	0.16585050090298512	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	811	0.15531727047612118	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	793	0.15187003142732933	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	773	0.14803976581756062	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	655	0.12544119871992523	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	646	0.12371757919552931	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	641	0.12276001279308713	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	606	0.1160570479759919	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	590	0.11299283548817693	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08215919732953882	0.0
2	0.0	0.0	0.0	0.5414080439408071	0.0
3	0.0	0.0	0.0	0.9248176314786549	0.0
4	0.0	0.0	0.0	1.683976275334813	0.0
5	0.0	0.0	0.0	3.906870921964084	0.0
6	0.0	0.0	0.0	5.2890222672491225	0.0
7	0.0	0.0	0.0	6.382946125399066	0.0
8	0.0	0.0	0.0	7.710707699025389	0.0
9	0.0	0.0	0.0	8.491698856857228	0.0
10	0.0	0.0	0.0	10.337886880765748	0.0
11	0.0	0.0	0.0	11.438322190452297	0.0
12	0.0	0.0	0.0	13.013518922469679	0.0
13	0.0	0.0	0.0	13.528881160264058	0.0
14	0.0	0.0	0.0	13.814810488033293	0.0
15	0.0	0.0	0.0	14.17753664127839	0.0
16	0.0	0.0	0.0	14.55807352960891	0.0
17	0.0	0.0	0.0	14.967911949854162	0.0
18	0.0	0.0	0.0	15.448801797160623	0.0
19	0.0	0.0	0.0	15.897708926625517	0.0
20	0.0	0.0	0.0	16.225962689382694	0.0
21	0.0	0.0	0.0	16.527021566310516	0.0
22	0.0	0.0	0.0	16.83957124006764	0.0
23	0.0	0.0	0.0	17.15441907319063	0.0
24	0.0	0.0	0.0	17.438816294715956	0.0
25	0.0	0.0	0.0	17.687783559350923	0.0
26	0.0	0.0	0.0	17.94900767393715	0.0
27	1.9151328048843546E-4	0.0	0.0	18.228617063450265	0.0
28	1.9151328048843546E-4	0.0	0.0	18.48735150539014	0.0
29	1.9151328048843546E-4	0.0	0.0	18.75566161135444	0.0
30	1.9151328048843546E-4	0.0	0.0	19.067828258550588	0.0
31	1.9151328048843546E-4	0.0	0.0	19.331159019222188	0.0
32	1.9151328048843546E-4	0.0	0.0	19.60176728455235	0.0
33	1.9151328048843546E-4	0.0	0.0	19.856288434321478	0.0
34	1.9151328048843546E-4	0.0	0.0	20.10717083176133	0.0
35	1.9151328048843546E-4	0.0	0.0	20.374523371323185	0.0
36	1.9151328048843546E-4	0.0	0.0	20.629044521092315	0.0
37	1.9151328048843546E-4	0.0	0.0	20.89869522002003	0.0
38	1.9151328048843546E-4	0.0	0.0	21.188454813399034	0.0
39	3.8302656097687093E-4	0.0	0.0	21.49851481450981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAC	30	2.1632168E-6	45.000004	32
TATTGCG	20	7.029746E-4	45.0	1
ACGATAG	25	3.8878712E-5	45.0	1
CGTTTTT	8915	0.0	44.268085	1
TACGAAT	65	0.0	41.538464	12
GCTACGA	65	0.0	41.538464	10
CCTATGC	65	0.0	41.538464	35
ATTGCGG	50	1.0786607E-9	40.5	2
CACGACC	45	1.9248546E-8	40.0	27
ACGACCA	45	1.9248546E-8	40.0	28
CGGGCAC	40	3.4533878E-7	39.375	6
GCGATAC	40	3.4533878E-7	39.375	9
GTTTTTT	10115	0.0	39.238754	2
ACCCGTC	35	6.2417403E-6	38.571426	39
CACGTGA	35	6.2417403E-6	38.571426	43
CGCGTAA	35	6.2417403E-6	38.571426	31
AACAGCG	35	6.2417403E-6	38.571426	1
TCGCGTA	35	6.2417403E-6	38.571426	30
AACACGT	35	6.2417403E-6	38.571426	41
AACGGGC	65	9.094947E-12	38.076927	4
>>END_MODULE