FastQCFastQC Report
Sat 18 Jun 2016
SRR3551458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459436
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.7981525174344195No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC10090.21961709574347676No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC7040.15323135322438816No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT5310.11557648943487232No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5260.11448819857390366No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC4950.10774079523589793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA207.028859E-445.00000422
GCGATTA351.2097917E-745.0000049
AATTCGG207.028859E-445.0000042
TACTACG207.028859E-445.0000041
TATACGG207.028859E-445.0000042
TATCGCG207.028859E-445.0000041
TCGTTTA253.8871367E-545.038
CGCATGG551.8189894E-1245.02
CATCGTT253.8871367E-545.036
CCGGTAT253.8871367E-545.042
CGGTATT253.8871367E-545.043
GCATCGT253.8871367E-545.035
TACCGGT253.8871367E-545.040
TAGCACG302.1626456E-644.9999961
GCGATAT302.1626456E-644.9999969
GGCGATT1150.043.0434768
TATTAGT700.041.78571730
ACTACGG501.0786607E-940.52
CGCTTGG501.0786607E-940.52
CGTTTTT16650.040.4054031