##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459436
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22979043871181	33.0	31.0	34.0	30.0	34.0
2	32.27857198826387	34.0	31.0	34.0	30.0	34.0
3	32.264528682993934	34.0	31.0	34.0	30.0	34.0
4	35.82600188056661	37.0	35.0	37.0	35.0	37.0
5	35.9067748282677	37.0	35.0	37.0	35.0	37.0
6	35.908944880244476	37.0	35.0	37.0	35.0	37.0
7	36.034995080925306	37.0	35.0	37.0	35.0	37.0
8	35.929341627560746	37.0	35.0	37.0	35.0	37.0
9	37.802980175693676	39.0	38.0	39.0	35.0	39.0
10	37.47035495694721	39.0	37.0	39.0	35.0	39.0
11	37.49136985347252	39.0	37.0	39.0	35.0	39.0
12	37.398157741230555	39.0	37.0	39.0	35.0	39.0
13	37.398529936705	39.0	37.0	39.0	35.0	39.0
14	38.645602434288996	40.0	38.0	41.0	35.0	41.0
15	38.705373544955116	40.0	38.0	41.0	35.0	41.0
16	38.694436221802384	40.0	38.0	41.0	35.0	41.0
17	38.62469636684979	40.0	38.0	41.0	35.0	41.0
18	38.49243202535283	40.0	38.0	41.0	34.0	41.0
19	38.45622241182667	40.0	37.0	41.0	34.0	41.0
20	38.3617892372387	40.0	37.0	41.0	34.0	41.0
21	38.2499499386204	40.0	37.0	41.0	34.0	41.0
22	38.23933910272595	40.0	37.0	41.0	34.0	41.0
23	38.22492577856328	40.0	37.0	41.0	34.0	41.0
24	38.188729224527464	40.0	37.0	41.0	34.0	41.0
25	37.97234652922278	40.0	36.0	41.0	34.0	41.0
26	38.057603235271074	40.0	36.0	41.0	34.0	41.0
27	38.02762952837827	40.0	36.0	41.0	34.0	41.0
28	37.816525043749294	40.0	36.0	41.0	33.0	41.0
29	37.61134956773087	40.0	36.0	41.0	33.0	41.0
30	37.49300011318225	40.0	35.0	41.0	33.0	41.0
31	37.50425957042983	40.0	35.0	41.0	33.0	41.0
32	37.46064740246737	40.0	35.0	41.0	33.0	41.0
33	37.33145421778006	40.0	35.0	41.0	33.0	41.0
34	37.180279734282905	40.0	35.0	41.0	32.0	41.0
35	37.07475034607649	40.0	35.0	41.0	32.0	41.0
36	36.9616029218433	40.0	35.0	41.0	31.0	41.0
37	36.90464395476192	40.0	35.0	41.0	31.0	41.0
38	36.7525400708695	39.0	35.0	41.0	31.0	41.0
39	36.68197311486257	39.0	35.0	41.0	31.0	41.0
40	36.6309083310842	39.0	35.0	41.0	31.0	41.0
41	36.49129149653053	39.0	35.0	41.0	30.0	41.0
42	36.38586223108333	39.0	35.0	41.0	30.0	41.0
43	36.219403790734724	39.0	35.0	41.0	30.0	41.0
44	36.25072697829513	39.0	35.0	41.0	30.0	41.0
45	36.228671675706735	39.0	35.0	41.0	30.0	41.0
46	36.09940884040432	39.0	35.0	41.0	30.0	41.0
47	36.10444109734544	38.0	35.0	41.0	30.0	41.0
48	36.02548124221872	38.0	35.0	41.0	30.0	41.0
49	35.87051515336195	38.0	35.0	41.0	29.0	41.0
50	35.732221680495215	38.0	35.0	41.0	29.0	41.0
51	34.92253327993453	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	7.0
11	5.0
12	7.0
13	9.0
14	13.0
15	19.0
16	33.0
17	75.0
18	125.0
19	200.0
20	390.0
21	585.0
22	952.0
23	1401.0
24	2070.0
25	3146.0
26	4484.0
27	5356.0
28	5846.0
29	6008.0
30	6677.0
31	8005.0
32	9870.0
33	13428.0
34	25309.0
35	34846.0
36	32581.0
37	48486.0
38	84350.0
39	164970.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.499064069859564	20.120321437588697	23.699710079314638	13.6809044132371
2	34.84337317928939	23.940875334105293	23.796785624113042	17.41896586249227
3	32.413872661262936	23.190607614553496	27.847839524982803	16.54768019920076
4	28.98662708190042	24.806066568575382	27.049034033031806	19.158272316492393
5	24.825873462245013	29.536431624861788	25.880209648351453	19.757485264541742
6	25.55676960447157	34.47640150097075	25.03199575131248	14.934833143245196
7	77.34918465248695	6.9998868177504585	10.848300960307855	4.802627569454723
8	77.8722172402685	6.490566694817124	10.48590010360529	5.151315961309083
9	71.01228462723861	9.467042199566425	12.586083807102622	6.9345893660923394
10	37.66291714188701	26.211703044602512	20.35909245248522	15.766287361025258
11	29.554279594981676	26.193419758138237	24.579484411321708	19.67281623555838
12	26.70426348827693	21.323100497131264	31.741526567356498	20.23110944723531
13	26.450691717671234	22.542421577760557	30.715268285463047	20.291618419105166
14	21.555559425034172	27.271045368669412	30.039874977145892	21.133520229150523
15	20.90998528630756	24.23144899398393	34.725184791788195	20.13338092792032
16	22.749632157688993	24.831314916549857	30.806684717784417	21.612368207976736
17	23.0341113887462	22.38657832646985	28.796176181230898	25.783134103553053
18	25.75440322482348	22.370253963555314	29.15226495093985	22.723077860681357
19	26.50967708233573	23.939569385072133	27.859593066281267	21.69116046631087
20	25.486466014852994	24.088665233024837	27.461278611166733	22.963590140955432
21	26.02429935834371	24.644128888463246	28.795523206714318	20.536048546478728
22	23.14076389312113	24.91903115994393	27.116290408239667	24.82391453869527
23	22.876091555733552	24.53660575139954	27.667618558406392	24.919684134460514
24	23.02518740368626	24.039474486109054	27.456272473206276	25.479065636998406
25	22.51216709182563	25.096422570281824	27.417964634900184	24.973445702992365
26	21.036227026179922	25.31299245161459	27.5701076972636	26.080672824941885
27	23.563891379865748	24.056669481712362	28.26683150645574	24.11260763196615
28	23.37039326478552	24.893565153797265	28.760697899163322	22.975343682253893
29	23.3536335855266	24.22992538677857	28.864085530955347	23.55235549673948
30	24.776900373501423	24.837844661715668	27.316971243002293	23.06828372178062
31	23.61242915226495	28.17367380875682	25.413768185340285	22.80012885363794
32	23.797220940457432	27.523093532069755	26.096561871512026	22.583123655960787
33	24.598420672302563	25.308856946342907	27.253850373066108	22.838872008288426
34	23.68229742553914	26.269382460233853	26.35840465266109	23.68991546156592
35	23.31445511453173	26.51620682750154	26.313784727361373	23.85555333060535
36	23.416319139118396	26.832899468043426	27.48826822451876	22.262513168319416
37	23.09048485534438	28.721301769996256	26.42283147163043	21.76538190302893
38	23.70166900286438	27.584037820284003	24.930567043070198	23.783726133781418
39	25.752226643101544	24.524416893756694	26.62416528090964	23.09919118223213
40	24.88812369949242	24.734892346268033	27.648029322908958	22.728954631330588
41	21.524869622754856	26.03779416501972	27.21684848379317	25.22048772843225
42	22.910699205112355	26.073707763431685	27.824985416902464	23.190607614553496
43	22.832559921294802	25.25988385759932	28.352153509955684	23.55540271115019
44	22.906128383496288	24.281510373588485	26.93149862004719	25.88086262286804
45	23.246981081151674	24.158968822643416	26.854229968918414	25.7398201272865
46	23.633541994967743	26.130734204546446	26.594346111319094	23.641377689166717
47	21.410599082353148	25.99056234165368	28.05831497749415	24.54052359849903
48	21.839168023402607	24.936661471891625	28.27031403721084	24.95385646749493
49	23.638765791100393	24.288910751443073	29.00730460825882	23.065018849197713
50	22.06749144603383	24.20641830418165	29.175336717192383	24.550753532592136
51	22.41422091433845	24.850468835702905	27.144585970624853	25.59072427933379
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	600.5
2	1005.0
3	3361.0
4	5717.0
5	3742.5
6	1768.0
7	1745.0
8	1722.0
9	1666.0
10	1610.0
11	1642.0
12	1674.0
13	1628.0
14	1582.0
15	1583.0
16	1584.0
17	1513.0
18	1442.0
19	1522.5
20	1603.0
21	1653.5
22	1704.0
23	1738.0
24	1772.0
25	2085.0
26	3022.0
27	3646.0
28	4268.5
29	4891.0
30	5519.0
31	6147.0
32	6725.5
33	7304.0
34	8273.0
35	9242.0
36	9774.5
37	10307.0
38	11553.5
39	12800.0
40	15463.5
41	18127.0
42	20260.0
43	22393.0
44	27129.5
45	31866.0
46	35657.0
47	39448.0
48	39595.0
49	39742.0
50	35837.0
51	31932.0
52	29480.0
53	27028.0
54	26251.5
55	25475.0
56	25066.0
57	24657.0
58	24920.0
59	25183.0
60	24541.0
61	23899.0
62	21706.0
63	19513.0
64	16716.0
65	13919.0
66	12008.5
67	10098.0
68	8834.0
69	7570.0
70	6742.0
71	5914.0
72	5220.0
73	4526.0
74	3942.5
75	2767.5
76	2176.0
77	1705.5
78	1235.0
79	973.0
80	711.0
81	535.0
82	359.0
83	241.0
84	123.0
85	76.0
86	29.0
87	25.5
88	22.0
89	16.5
90	11.0
91	8.5
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	459436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.610686278028794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86594098776617	25.911615964369922
2	8.204622251202606	5.679352135322156
3	2.9089856720901013	3.0204597145197374
4	1.7111767142089878	2.369000016870217
5	1.2674480894090776	2.193362409811229
6	1.020826852467261	2.1198910760959175
7	0.9114390050432802	2.2081870625578173
8	0.8150206231302357	2.25667384778273
9	0.6676674516125733	2.0797585835232373
>10	7.51150345816991	46.61797977286618
>50	0.07691259659986859	1.8016488062436042
>100	0.03530414270157903	2.337539501185386
>500	0.001891293359013162	0.3842441017070349
>1k	0.001260862239342108	1.020287007144858
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3667	0.7981525174344195	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	1009	0.21961709574347676	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC	704	0.15323135322438816	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	531	0.11557648943487232	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	526	0.11448819857390366	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	495	0.10774079523589793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12036496922313446	0.0
2	0.0	0.0	0.0	0.6024778206322534	0.0
3	0.0	0.0	0.0	0.894139771371856	0.0
4	0.0	0.0	0.0	1.259587842485134	0.0
5	0.0	0.0	0.0	2.2745278994245117	0.0
6	0.0	0.0	0.0	2.9372970337544295	0.0
7	0.0	0.0	0.0	3.5482635231022384	0.0
8	0.0	0.0	0.0	4.50334758268834	0.0
9	0.0	0.0	0.0	4.9347460799763185	0.0
10	0.0	0.0	0.0	5.862622868038203	0.0
11	0.0	0.0	0.0	7.0358004161624255	0.0
12	0.0	0.0	0.0	8.026798074160492	0.0
13	0.0	0.0	0.0	8.57769090798283	0.0
14	0.0	0.0	0.0	8.87501197119947	0.0
15	0.0	0.0	0.0	9.200193280456908	0.0
16	0.0	0.0	0.0	9.812901035182266	0.0
17	0.0	0.0	0.0	10.495912379526201	0.0
18	0.0	0.0	0.0	11.350220705386604	0.0
19	0.0	0.0	0.0	11.779224962780452	0.0
20	0.0	0.0	0.0	12.207358587485526	0.0
21	0.0	0.0	0.0	12.69208333696097	0.0
22	0.0	0.0	0.0	13.19987985268895	0.0
23	0.0	0.0	0.0	13.709417633794478	0.0
24	0.0	0.0	0.0	14.112955885041659	0.0
25	0.0	0.0	0.0	14.489069206592431	0.0
26	2.176581721937332E-4	0.0	0.0	14.84755221619551	0.0
27	2.176581721937332E-4	0.0	0.0	15.20320566956007	0.0
28	2.176581721937332E-4	0.0	0.0	15.551241086897848	0.0
29	2.176581721937332E-4	0.0	0.0	15.931054597375914	0.0
30	2.176581721937332E-4	0.0	0.0	16.332416266901156	0.0
31	2.176581721937332E-4	0.0	0.0	16.700258577908567	0.0
32	2.176581721937332E-4	0.0	0.0	17.095525818612387	0.0
33	2.176581721937332E-4	0.0	0.0	17.527359632244753	0.0
34	2.176581721937332E-4	0.0	0.0	17.970076354486807	0.0
35	2.176581721937332E-4	0.0	0.0	18.389285994131935	0.0
36	2.176581721937332E-4	0.0	0.0	18.763005075788577	0.0
37	2.176581721937332E-4	0.0	0.0	19.136724157445215	0.0
38	2.176581721937332E-4	0.0	0.0	19.513925769856954	0.0
39	4.353163443874664E-4	0.0	0.0	19.973837487702312	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	20	7.028859E-4	45.000004	22
GCGATTA	35	1.2097917E-7	45.000004	9
AATTCGG	20	7.028859E-4	45.000004	2
TACTACG	20	7.028859E-4	45.000004	1
TATACGG	20	7.028859E-4	45.000004	2
TATCGCG	20	7.028859E-4	45.000004	1
TCGTTTA	25	3.8871367E-5	45.0	38
CGCATGG	55	1.8189894E-12	45.0	2
CATCGTT	25	3.8871367E-5	45.0	36
CCGGTAT	25	3.8871367E-5	45.0	42
CGGTATT	25	3.8871367E-5	45.0	43
GCATCGT	25	3.8871367E-5	45.0	35
TACCGGT	25	3.8871367E-5	45.0	40
TAGCACG	30	2.1626456E-6	44.999996	1
GCGATAT	30	2.1626456E-6	44.999996	9
GGCGATT	115	0.0	43.043476	8
TATTAGT	70	0.0	41.785717	30
ACTACGG	50	1.0786607E-9	40.5	2
CGCTTGG	50	1.0786607E-9	40.5	2
CGTTTTT	1665	0.0	40.405403	1
>>END_MODULE