FastQCFastQC Report
Sat 18 Jun 2016
SRR3551456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333357
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45121.3535039012230132No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC8880.2663810869428271No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC7020.21058504846155923No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC6900.20698530404341292No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT6600.19798594299804714Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3940.1181916083958039No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC3710.11129209826102346No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG3550.10649243903682838No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3490.10469256682775523No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT3430.10289269461868208No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC3400.10199275851414549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCG351.2084638E-745.00000436
ACCGGTA207.0260727E-445.041
GCGAATG207.0260727E-445.01
TACGGGC253.884828E-545.04
CTGCGAT207.0260727E-445.018
CGGTCTA253.884828E-545.031
CGCAATG207.0260727E-445.02
ATACCGG207.0260727E-445.02
CTAGACG302.1608485E-644.99999626
CATATTA302.1608485E-644.99999628
TAGGGTG800.042.18755
CGTTTTT20300.041.120691
TCGTTCA451.9212166E-840.016
CTATAGG451.9212166E-840.02
TGCGTAG451.9212166E-840.01
CAAACGG356.23495E-638.571432
AGATCGG356.23495E-638.571432
TCGATGG356.23495E-638.571432
CTTGGCG356.23495E-638.571431
TGTTGCG356.23495E-638.571431
Produced by FastQC (version 0.11.5)