##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333357
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26988483817649	33.0	31.0	34.0	30.0	34.0
2	32.359509474827284	34.0	31.0	34.0	30.0	34.0
3	32.3391589197167	34.0	31.0	34.0	30.0	34.0
4	35.916086957825996	37.0	35.0	37.0	35.0	37.0
5	35.979265472151475	37.0	35.0	37.0	35.0	37.0
6	35.959145900641055	37.0	35.0	37.0	35.0	37.0
7	36.134849425690774	37.0	36.0	37.0	35.0	37.0
8	35.98608998761088	37.0	35.0	37.0	35.0	37.0
9	37.87449491086133	39.0	38.0	39.0	35.0	39.0
10	37.51432548289071	39.0	37.0	39.0	35.0	39.0
11	37.5254516929298	39.0	37.0	39.0	35.0	39.0
12	37.487069418071314	39.0	37.0	39.0	35.0	39.0
13	37.50020248562352	39.0	37.0	39.0	35.0	39.0
14	38.76548865030583	40.0	38.0	41.0	35.0	41.0
15	38.82373551477845	40.0	38.0	41.0	35.0	41.0
16	38.7993442465585	40.0	38.0	41.0	35.0	41.0
17	38.76576463071122	40.0	38.0	41.0	35.0	41.0
18	38.62542559478277	40.0	38.0	41.0	35.0	41.0
19	38.49294000125991	40.0	37.0	41.0	35.0	41.0
20	38.375276355378766	40.0	37.0	41.0	34.0	41.0
21	38.268840912295225	40.0	37.0	41.0	34.0	41.0
22	38.281080043316926	40.0	37.0	41.0	34.0	41.0
23	38.24240078954394	40.0	37.0	41.0	34.0	41.0
24	38.17518756168312	40.0	37.0	41.0	34.0	41.0
25	37.96348959223895	40.0	36.0	41.0	34.0	41.0
26	37.99040668112564	40.0	36.0	41.0	34.0	41.0
27	37.989737728621265	40.0	36.0	41.0	34.0	41.0
28	37.916302942491086	40.0	36.0	41.0	34.0	41.0
29	37.843048143581804	40.0	36.0	41.0	34.0	41.0
30	37.64563815969066	40.0	36.0	41.0	33.0	41.0
31	37.54520529042438	40.0	35.0	41.0	33.0	41.0
32	37.38311179906227	40.0	35.0	41.0	33.0	41.0
33	37.18461589227165	40.0	35.0	41.0	32.0	41.0
34	36.970176117495654	40.0	35.0	41.0	31.0	41.0
35	36.81717498057638	40.0	35.0	41.0	31.0	41.0
36	36.69354175853515	39.0	35.0	41.0	31.0	41.0
37	36.63372600545361	39.0	35.0	41.0	31.0	41.0
38	36.54364540117652	39.0	35.0	41.0	30.0	41.0
39	36.47590121101402	39.0	35.0	41.0	30.0	41.0
40	36.36128534874024	39.0	35.0	41.0	30.0	41.0
41	36.267503007286486	39.0	35.0	41.0	30.0	41.0
42	36.1863917661846	39.0	35.0	41.0	30.0	41.0
43	36.099784915271016	39.0	35.0	41.0	30.0	41.0
44	36.13105169532963	39.0	35.0	41.0	30.0	41.0
45	36.0988099844911	39.0	35.0	41.0	30.0	41.0
46	36.015640889496844	39.0	35.0	41.0	30.0	41.0
47	35.929891977669584	39.0	35.0	41.0	29.0	41.0
48	35.86205479410962	38.0	35.0	41.0	29.0	41.0
49	35.807983633162046	38.0	35.0	41.0	29.0	41.0
50	35.73055913030175	38.0	35.0	40.0	29.0	41.0
51	34.89639335607172	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	6.0
11	11.0
12	13.0
13	11.0
14	14.0
15	16.0
16	38.0
17	63.0
18	78.0
19	151.0
20	251.0
21	469.0
22	620.0
23	1015.0
24	1556.0
25	2433.0
26	3535.0
27	4351.0
28	4393.0
29	4372.0
30	4751.0
31	5551.0
32	6813.0
33	9466.0
34	17019.0
35	24072.0
36	25096.0
37	36643.0
38	65830.0
39	114589.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.975632730076164	20.503244269656854	23.419037248355366	17.102085751911613
2	35.11820660733088	24.631551159867648	23.85760610996619	16.392636122835277
3	30.94280306098267	23.19645305183332	29.832581886686043	16.028162000497964
4	28.43078141451957	24.317473459384384	28.97914248088386	18.272602645212192
5	24.660649093914333	28.588870190216493	27.437851912514212	19.31262880335496
6	24.674748092885405	33.25803867925377	26.82319555311573	15.244017674745091
7	74.66979844431046	6.664026854093359	13.463944059971741	5.202230641624444
8	75.28805454812708	6.167862081792193	13.057172940721209	5.486910429359516
9	69.0760955972126	7.976433673209201	15.417705342920653	7.529765386657547
10	36.5776029901877	23.183253988966783	23.625722573697267	16.613420447148254
11	29.175928509075856	23.651220763325803	26.333030354844805	20.839820372753532
12	24.258977612589504	21.178196348059288	32.73067612199534	21.832149917355867
13	23.476633159045708	22.22062233581416	33.86849533683108	20.434249168309048
14	20.88181739096524	25.125316102556717	32.165516248346364	21.827350258131673
15	20.373353491902073	23.67401914464073	34.56504588174239	21.387581481714797
16	21.729257222737186	23.013766022612394	33.028854951298456	22.22812180335196
17	22.18672474254328	23.25794868564332	30.835410685841307	23.719915885972096
18	22.001337905008743	23.822808580590777	31.642953350312126	22.53290016408835
19	22.853877374706396	24.270976760649994	30.02936791487804	22.845777949765566
20	23.21415179522254	25.04342191704389	30.38334278266243	21.359083505071137
21	24.08858971013058	24.699346346409403	30.43853886374067	20.77352507971934
22	22.44410646844074	23.530029367914878	28.88344927510147	25.142414888542913
23	22.147727511346694	24.47556223508131	29.831381971879996	23.545328281691997
24	22.357412623703716	24.438064897392287	30.325746871972093	22.87877560693191
25	22.315115626790497	25.449593078891397	28.950044546837173	23.28524674748093
26	21.23489232264509	25.10221774254028	29.70899066166302	23.953899273151606
27	22.89017479758937	24.65314962637653	29.61029766886551	22.84637790716859
28	21.58616738211587	24.432665280765065	30.01676880941453	23.96439852770453
29	22.64509219845391	23.727715332211414	28.92904603773132	24.69814643160336
30	22.891974669798447	25.31070294009125	28.230995599312447	23.566326790797852
31	24.0078954394238	24.885933098749987	26.571513422547	24.53465803927921
32	24.141385961596725	25.80536781888486	26.705303923421436	23.347942296096978
33	24.43326523816809	25.080619276031403	26.596711633474023	23.889403852326485
34	22.79778135752362	24.350171137849212	28.14910141379962	24.702946090827552
35	24.85893501561389	23.549527983513173	27.2161676520967	24.37536934877624
36	24.521758955114187	25.44869314278686	27.562943031044796	22.466604871054155
37	23.366540975590734	25.874662898934176	28.585870403201376	22.172925722273717
38	24.376569263582283	25.178412332724378	27.463350102142748	22.981668301550588
39	24.740143449815065	23.30594527788527	28.993541458556443	22.960369813743224
40	24.141385961596725	23.593924831336974	30.53483202692609	21.72985718014021
41	22.51340154849006	24.303074481711796	29.39131321676161	23.792210753036535
42	22.31391571198445	24.910231373572476	29.3664149845361	23.409437929906975
43	23.1910534352061	23.790410880827462	29.31361873306995	23.704916950896486
44	23.231550559910247	23.570826471320537	28.439780775564934	24.757842193204283
45	23.440635714864243	23.2402499422541	28.259193597254594	25.05992074562706
46	24.036093437365945	23.859405982175268	28.58017080787264	23.524329772586146
47	22.61209454128757	24.32647282042975	29.82388250434219	23.23755013394049
48	22.000137990202695	23.77361207354278	29.56350099142961	24.662748944824916
49	22.82267958974913	23.00326676805947	30.585828406183165	23.588225236008242
50	21.938342377691182	22.72578646916069	30.468536733891895	24.86733441925623
51	22.535000014998936	22.549099013970007	28.79735538776747	26.118545583263586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	489.5
2	741.0
3	3676.5
4	6612.0
5	4224.5
6	1837.0
7	1766.0
8	1695.0
9	1712.0
10	1729.0
11	1707.5
12	1686.0
13	1677.5
14	1669.0
15	1603.0
16	1537.0
17	1446.5
18	1356.0
19	1354.0
20	1352.0
21	1387.5
22	1423.0
23	1576.0
24	1729.0
25	1957.5
26	2452.0
27	2718.0
28	3201.0
29	3684.0
30	4340.5
31	4997.0
32	5459.5
33	5922.0
34	6387.0
35	6852.0
36	7177.0
37	7502.0
38	7982.0
39	8462.0
40	9820.0
41	11178.0
42	12320.5
43	13463.0
44	15097.0
45	16731.0
46	18426.0
47	20121.0
48	23884.5
49	27648.0
50	29012.5
51	30377.0
52	29916.0
53	29455.0
54	25678.5
55	21902.0
56	20278.0
57	18654.0
58	17307.0
59	15960.0
60	15251.5
61	14543.0
62	13483.5
63	12424.0
64	11154.0
65	9884.0
66	8531.5
67	7179.0
68	6378.0
69	5577.0
70	4803.0
71	4029.0
72	3621.0
73	3213.0
74	2701.0
75	1765.0
76	1341.0
77	1048.5
78	756.0
79	614.5
80	473.0
81	337.5
82	202.0
83	133.0
84	64.0
85	55.0
86	46.0
87	31.0
88	16.0
89	9.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	333357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.57267075088253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.08340218054477	28.12960070343263
2	8.641230029141987	6.8391310165174986
3	3.9383601172799683	4.675542846585818
4	2.781417334348303	4.40272449571851
5	2.3147844620897278	4.580110168876776
6	1.8428806129434285	4.375662463751693
7	1.6688846733894223	4.622955659086362
8	1.4655210365190967	4.639566516532985
9	1.2016623917186264	4.27976911630793
>10	4.971412867677693	27.02003607096809
>50	0.05168245391254806	1.4142044510552212
>100	0.034961659999664864	2.7793839498514035
>500	0.0030401443477969447	0.8842537401323587
>1k	7.600360869492362E-4	1.357058801182722
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4512	1.3535039012230132	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	888	0.2663810869428271	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	702	0.21058504846155923	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	690	0.20698530404341292	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	660	0.19798594299804714	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	394	0.1181916083958039	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	371	0.11129209826102346	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	355	0.10649243903682838	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	349	0.10469256682775523	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	343	0.10289269461868208	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	340	0.10199275851414549	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9997870151219264E-4	0.0	0.0	0.20638534664038855	0.0
2	2.9997870151219264E-4	0.0	0.0	0.8726380426989684	0.0
3	2.9997870151219264E-4	0.0	0.0	1.2407119094544288	0.0
4	2.9997870151219264E-4	0.0	0.0	1.7497757659206197	0.0
5	2.9997870151219264E-4	0.0	0.0	3.106279454158755	0.0
6	2.9997870151219264E-4	0.0	0.0	4.123807209688112	0.0
7	2.9997870151219264E-4	0.0	0.0	4.994945358879519	0.0
8	2.9997870151219264E-4	0.0	0.0	6.214958737929607	0.0
9	2.9997870151219264E-4	0.0	0.0	6.83111499083565	0.0
10	2.9997870151219264E-4	0.0	0.0	7.967434312163836	0.0
11	2.9997870151219264E-4	0.0	0.0	9.274441514652459	0.0
12	2.9997870151219264E-4	0.0	0.0	10.354064861394841	0.0
13	2.9997870151219264E-4	0.0	0.0	10.788134042482984	0.0
14	2.9997870151219264E-4	0.0	0.0	10.980120411450788	0.0
15	2.9997870151219264E-4	0.0	0.0	11.283998836082638	0.0
16	2.9997870151219264E-4	0.0	0.0	11.813161265550146	0.0
17	2.9997870151219264E-4	0.0	0.0	12.444616432233312	0.0
18	2.9997870151219264E-4	0.0	0.0	13.156165912220233	0.0
19	2.9997870151219264E-4	0.0	0.0	13.5539376704254	0.0
20	2.9997870151219264E-4	0.0	0.0	13.955909130451738	0.0
21	2.9997870151219264E-4	0.0	0.0	14.412776692854807	0.0
22	2.9997870151219264E-4	0.0	0.0	14.90024208281212	0.0
23	2.9997870151219264E-4	0.0	0.0	15.387707472769433	0.0
24	2.9997870151219264E-4	0.0	0.0	15.789078975392748	0.0
25	5.999574030243853E-4	0.0	0.0	16.17395164943289	0.0
26	5.999574030243853E-4	0.0	0.0	16.504828157200837	0.0
27	5.999574030243853E-4	0.0	0.0	16.87170210915025	0.0
28	5.999574030243853E-4	0.0	0.0	17.21667761588927	0.0
29	5.999574030243853E-4	0.0	0.0	17.543654400537562	0.0
30	5.999574030243853E-4	0.0	0.0	17.94682577536995	0.0
31	5.999574030243853E-4	0.0	0.0	18.281002048854532	0.0
32	5.999574030243853E-4	0.0	0.0	18.63497691663892	0.0
33	5.999574030243853E-4	0.0	0.0	18.977852572467356	0.0
34	5.999574030243853E-4	0.0	0.0	19.3258278662215	0.0
35	5.999574030243853E-4	0.0	0.0	19.714300284679787	0.0
36	5.999574030243853E-4	0.0	0.0	20.049076515567396	0.0
37	5.999574030243853E-4	0.0	0.0	20.403051383351784	0.0
38	5.999574030243853E-4	0.0	0.0	20.74232729476207	0.0
39	5.999574030243853E-4	0.0	0.0	21.07590361084363	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCG	35	1.2084638E-7	45.000004	36
ACCGGTA	20	7.0260727E-4	45.0	41
GCGAATG	20	7.0260727E-4	45.0	1
TACGGGC	25	3.884828E-5	45.0	4
CTGCGAT	20	7.0260727E-4	45.0	18
CGGTCTA	25	3.884828E-5	45.0	31
CGCAATG	20	7.0260727E-4	45.0	2
ATACCGG	20	7.0260727E-4	45.0	2
CTAGACG	30	2.1608485E-6	44.999996	26
CATATTA	30	2.1608485E-6	44.999996	28
TAGGGTG	80	0.0	42.1875	5
CGTTTTT	2030	0.0	41.12069	1
TCGTTCA	45	1.9212166E-8	40.0	16
CTATAGG	45	1.9212166E-8	40.0	2
TGCGTAG	45	1.9212166E-8	40.0	1
CAAACGG	35	6.23495E-6	38.57143	2
AGATCGG	35	6.23495E-6	38.57143	2
TCGATGG	35	6.23495E-6	38.57143	2
CTTGGCG	35	6.23495E-6	38.57143	1
TGTTGCG	35	6.23495E-6	38.57143	1
>>END_MODULE