##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	602891
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01016601674266	33.0	31.0	34.0	30.0	34.0
2	32.09603062576817	33.0	31.0	34.0	30.0	34.0
3	31.996246419336167	33.0	31.0	34.0	30.0	34.0
4	35.7103589206009	37.0	35.0	37.0	33.0	37.0
5	30.942900126225137	37.0	35.0	37.0	0.0	37.0
6	33.25984796588438	37.0	35.0	37.0	17.0	37.0
7	35.1753749848646	37.0	35.0	37.0	32.0	37.0
8	35.5556626322171	37.0	35.0	37.0	33.0	37.0
9	37.59531822501911	39.0	37.0	39.0	35.0	39.0
10	37.399468560651925	39.0	37.0	39.0	35.0	39.0
11	37.207135286477985	39.0	37.0	39.0	34.0	39.0
12	37.14441416441778	39.0	37.0	39.0	34.0	39.0
13	37.03739813664493	39.0	37.0	39.0	33.0	39.0
14	38.32286433202685	40.0	38.0	41.0	34.0	41.0
15	38.322476202165895	40.0	37.0	41.0	33.0	41.0
16	38.3141911224417	40.0	37.0	41.0	34.0	41.0
17	38.2442929153031	40.0	37.0	41.0	33.0	41.0
18	38.17463521598432	40.0	37.0	41.0	33.0	41.0
19	38.0783624237217	40.0	37.0	41.0	34.0	41.0
20	38.10105972721437	40.0	37.0	41.0	34.0	41.0
21	38.10893677298218	40.0	37.0	41.0	34.0	41.0
22	38.13636461648955	40.0	37.0	41.0	34.0	41.0
23	38.146392963238796	40.0	37.0	41.0	34.0	41.0
24	38.08850521901969	40.0	37.0	41.0	34.0	41.0
25	37.973787964988695	40.0	37.0	41.0	34.0	41.0
26	37.86886352591099	40.0	36.0	41.0	33.0	41.0
27	37.84205436803668	40.0	36.0	41.0	33.0	41.0
28	37.81086962651624	40.0	36.0	41.0	33.0	41.0
29	37.77364233335711	40.0	36.0	41.0	33.0	41.0
30	37.69365772585758	40.0	36.0	41.0	33.0	41.0
31	37.63505343420287	40.0	36.0	41.0	33.0	41.0
32	37.53802096896454	40.0	36.0	41.0	33.0	41.0
33	37.410458938680456	40.0	36.0	41.0	33.0	41.0
34	37.289621175303665	40.0	36.0	41.0	32.0	41.0
35	37.25249671997094	40.0	36.0	41.0	32.0	41.0
36	37.10331718337145	40.0	35.0	41.0	32.0	41.0
37	37.06971575293046	39.0	35.0	41.0	32.0	41.0
38	37.02638619584635	39.0	35.0	41.0	31.0	41.0
39	36.92352348932062	39.0	35.0	41.0	31.0	41.0
40	36.812307697411306	39.0	35.0	41.0	31.0	41.0
41	36.75975425076838	39.0	35.0	41.0	31.0	41.0
42	36.73622429261674	39.0	35.0	41.0	31.0	41.0
43	36.68864189380833	39.0	35.0	41.0	31.0	41.0
44	36.58836671968896	39.0	35.0	41.0	31.0	41.0
45	36.545566279808455	39.0	35.0	40.0	30.0	41.0
46	36.39922639415749	39.0	35.0	40.0	30.0	41.0
47	36.253679354974615	39.0	35.0	40.0	30.0	41.0
48	36.143730790474564	39.0	35.0	40.0	30.0	41.0
49	36.00352634224097	38.0	35.0	40.0	29.0	41.0
50	35.83231794802045	38.0	35.0	40.0	28.0	41.0
51	33.97230179252966	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	33.0
10	46.0
11	27.0
12	22.0
13	9.0
14	19.0
15	18.0
16	38.0
17	87.0
18	189.0
19	335.0
20	454.0
21	779.0
22	1114.0
23	1771.0
24	2463.0
25	3433.0
26	4444.0
27	5260.0
28	6069.0
29	7242.0
30	9435.0
31	12380.0
32	17115.0
33	24729.0
34	38077.0
35	48662.0
36	51513.0
37	78264.0
38	121266.0
39	167536.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55299548342901	21.370861399490124	23.110976942764115	10.965166174316751
2	30.390899847567805	29.714492337752596	23.681229276933973	16.21337853774563
3	30.904093774828283	28.782814804002715	25.44838121650514	14.864710204663862
4	28.604673149872863	24.93949984325525	29.622104161448753	16.833722845423136
5	24.320150740349415	38.4951840382424	20.360562688777904	16.824102532630274
6	25.254316286028487	37.544100011444854	23.848257811113452	13.353325891413206
7	76.99269022095204	6.027457699650517	12.420321417967758	4.559530661429678
8	75.66392598330378	8.98835776284602	9.923518513296765	5.424197740553433
9	69.09972117679646	9.037454531581995	11.228066101500934	10.634758190120602
10	38.181528667702786	29.1437424011969	18.465526936046484	14.209201995053833
11	30.03826562347091	25.862552268983947	25.741137286839578	18.358044820705565
12	28.37544431746369	22.232708731760802	29.25819094993954	20.133656000835973
13	24.29908557268229	23.280493488872782	32.861329825789404	19.559091112655523
14	20.703410732619993	29.455573229655112	27.583593054134166	22.257422983590732
15	18.98734597132815	27.182359663687134	34.9675148575779	18.862779507406813
16	21.350791436594672	27.44393265117575	29.21008938597524	21.995186526254333
17	20.773904403946982	26.870860570152814	29.226510264707883	23.12872476119232
18	21.40420075934124	28.938564350769873	28.051173429359537	21.60606146052935
19	23.04031740397518	29.060476935300077	26.107206775354086	21.791998885370656
20	24.860878666292912	28.05133929682148	28.495532359912488	18.59224967697312
21	23.83913510070643	29.922158400108813	27.402963388075126	18.835743111109636
22	21.623311676571717	25.66616519404005	27.523714900371708	25.18680822901652
23	21.79050607821314	29.27560703344386	27.948335602953104	20.985551285389896
24	23.642416290838643	25.802674115221492	26.95114042173461	23.603769172205258
25	21.233357273537006	30.974421578693327	25.463641023004158	22.328580124765505
26	20.298528258010155	28.51427538311237	27.750455720851697	23.43674063802578
27	22.810756836642113	30.044568587024852	25.655217941551623	21.489456634781412
28	20.058352173112553	28.451245747572944	29.02481543098172	22.465586648332785
29	22.879425965887698	26.15829395363341	28.13626343733776	22.82601664314113
30	21.686673047035036	29.692597832775743	27.082673319057676	21.53805580113155
31	26.106543305506303	27.98681685412454	23.833993209386108	22.072646630983048
32	27.25285333501412	29.309941598066647	24.073837559359816	19.36336750755941
33	23.236870346381018	28.76573045542229	23.09920035296596	24.89819884523073
34	23.658671302109337	28.57166552494564	26.505620418948034	21.264042753996993
35	24.547720898139133	27.687757820236165	24.445878276504377	23.31864300512033
36	23.3518164975095	30.712019253894983	25.64261201444374	20.29355223415178
37	24.537105380574598	30.117716137742974	26.439936904017475	18.905241577664953
38	24.835998547001033	27.701193084653774	23.64573364007756	23.81707472826763
39	26.18964290394118	27.456372710821693	25.239388214453356	21.114596170783773
40	23.68935678256932	26.17023641089351	28.345090571927596	21.795316234609572
41	20.879561977206492	31.732601747247845	25.645431761296816	21.742404514248843
42	23.347669810960852	27.499498250927612	28.39468494304941	20.758146995062127
43	24.7592019121201	27.815973368320314	25.77099342998983	21.653831289569755
44	22.8092640294846	26.616917485913703	26.712623011456465	23.861195473145226
45	22.23022071983161	25.23889061206752	26.052802247835842	26.478086420265022
46	24.82770517390374	27.864904269594337	25.257136032881565	22.050254523620357
47	21.261057139681967	25.70514404759733	31.24826875836594	21.78553005435477
48	22.692161601350826	26.052304645450008	26.908180749090633	24.347353004108538
49	21.738257827700195	24.509903116815476	30.40533031675709	23.34650873872723
50	21.565755667276505	24.541749669509084	29.040241104942684	24.852253558271727
51	21.0915405935733	23.911619181576768	26.391337737667342	28.605502487182594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	142.0
1	422.5
2	703.0
3	2114.5
4	3526.0
5	2402.0
6	1278.0
7	1138.0
8	998.0
9	1020.5
10	1043.0
11	1001.5
12	960.0
13	976.0
14	992.0
15	1033.0
16	1074.0
17	1073.5
18	1073.0
19	1140.0
20	1207.0
21	1504.5
22	1802.0
23	2146.0
24	2490.0
25	2990.0
26	4185.5
27	4881.0
28	6028.5
29	7176.0
30	8763.5
31	10351.0
32	12131.5
33	13912.0
34	15184.0
35	16456.0
36	18620.0
37	20784.0
38	21830.0
39	22876.0
40	25772.0
41	28668.0
42	31342.0
43	34016.0
44	39690.5
45	45365.0
46	62299.5
47	79234.0
48	70121.0
49	61008.0
50	58624.0
51	56240.0
52	47417.0
53	38594.0
54	33899.0
55	29204.0
56	26661.0
57	24118.0
58	23207.5
59	22297.0
60	21522.5
61	20748.0
62	18918.0
63	17088.0
64	13778.5
65	10469.0
66	8392.5
67	6316.0
68	5187.0
69	4058.0
70	3451.0
71	2844.0
72	2479.0
73	2114.0
74	1780.5
75	1128.5
76	810.0
77	665.0
78	520.0
79	373.5
80	227.0
81	177.0
82	127.0
83	83.0
84	39.0
85	53.0
86	67.0
87	46.0
88	25.0
89	18.5
90	12.0
91	11.0
92	10.0
93	9.0
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	602891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.49152474673505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58834700527579	25.381742918037965
2	10.042672150314932	6.927741499918688
3	3.871086994881507	4.005590786421592
4	2.1177220471443086	2.9217384958313737
5	1.478914743499761	2.550501223686666
6	1.0893571830398736	2.2544154142112034
7	0.8204793372576008	1.9809708355643725
8	0.6297522023947495	1.7376890938567524
9	0.5654181294751413	1.7551920064540032
>10	5.641490786611789	39.394744156304576
>50	0.10691581388947528	2.3708980512960327
>100	0.03905600507350634	2.37729678181869
>500	0.003905600507350634	1.0185768687647867
>1k	0.003417400443931805	1.766386403263781
>5k	0.0014646001902564878	3.5565154645695256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	7609	1.2620855179460302	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	7233	1.1997193522543876	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	6279	1.0414817935580396	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	2262	0.3751921989215297	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1908	0.3164751173926962	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	1610	0.2670466137328307	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT	1400	0.23221444672420055	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTC	1141	0.18925477408022345	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1102	0.18278594306433502	Illumina Single End Adapter 1 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	1067	0.17698058189623	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	983	0.16304771509277796	Illumina Single End Adapter 1 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	943	0.15641301661494367	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	929	0.15409087214770165	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTA	739	0.12257605437798873	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTG	725	0.1202539099107467	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTT	706	0.11710242813377542	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.976023858375726E-4	0.0	0.0	0.19622120748194946	0.0
2	6.634698477834301E-4	0.0	0.0	1.0517655761986826	0.0
3	6.634698477834301E-4	0.0	0.0	1.4379050276086391	0.0
4	6.634698477834301E-4	0.0	0.0	2.1242645851405975	0.0
5	6.634698477834301E-4	0.0	0.0	4.672652270476753	0.0
6	6.634698477834301E-4	0.0	0.0	5.684443788346484	0.0
7	6.634698477834301E-4	0.0	0.0	6.803385686633239	0.0
8	6.634698477834301E-4	0.0	0.0	8.31808734912281	0.0
9	6.634698477834301E-4	0.0	0.0	8.844550673338961	0.0
10	6.634698477834301E-4	0.0	0.0	11.095869734330087	0.0
11	6.634698477834301E-4	0.0	0.0	12.430605200608403	0.0
12	6.634698477834301E-4	0.0	0.0	14.982641970107366	0.0
13	6.634698477834301E-4	0.0	0.0	15.585404326818612	0.0
14	6.634698477834301E-4	0.0	0.0	15.87981907177251	0.0
15	6.634698477834301E-4	0.0	0.0	16.39798902289137	0.0
16	6.634698477834301E-4	0.0	0.0	17.175741551955493	0.0
17	6.634698477834301E-4	0.0	0.0	18.006239933918405	0.0
18	6.634698477834301E-4	0.0	0.0	18.86974594080854	0.0
19	6.634698477834301E-4	0.0	0.0	19.464878394270276	0.0
20	6.634698477834301E-4	0.0	0.0	20.054205486563905	0.0
21	6.634698477834301E-4	0.0	0.0	20.72182202089598	0.0
22	6.634698477834301E-4	0.0	0.0	21.477680044983256	0.0
23	6.634698477834301E-4	0.0	0.0	22.15607796434181	0.0
24	6.634698477834301E-4	0.0	0.0	22.705762733230387	0.0
25	6.634698477834301E-4	0.0	0.0	23.189763987188396	0.0
26	6.634698477834301E-4	0.0	0.0	23.657510229875715	0.0
27	6.634698477834301E-4	0.0	0.0	24.14499470053459	0.0
28	6.634698477834301E-4	0.0	0.0	24.630654629112062	0.0
29	6.634698477834301E-4	0.0	0.0	25.146170700839786	0.0
30	6.634698477834301E-4	0.0	0.0	25.739312744758173	0.0
31	8.293373097292878E-4	0.0	0.0	26.266605406284054	0.0
32	8.293373097292878E-4	0.0	0.0	26.783448417707348	0.0
33	8.293373097292878E-4	0.0	0.0	27.243067154759316	0.0
34	8.293373097292878E-4	0.0	0.0	27.749626383541965	0.0
35	8.293373097292878E-4	0.0	0.0	28.27210888867142	0.0
36	8.293373097292878E-4	0.0	0.0	28.784639346084116	0.0
37	8.293373097292878E-4	0.0	0.0	29.273782491362454	0.0
38	8.293373097292878E-4	0.0	0.0	29.785483611465423	0.0
39	8.293373097292878E-4	0.0	0.0	30.292374575171962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	45	3.8380676E-10	45.000004	4
CGGCCGT	20	7.0306147E-4	45.0	21
TCCGACG	20	7.0306147E-4	45.0	27
ACATTCG	20	7.0306147E-4	45.0	23
TAGCCGT	20	7.0306147E-4	45.0	44
TCGTCGG	20	7.0306147E-4	45.0	2
TTGCGAC	20	7.0306147E-4	45.0	1
ATGCGAT	25	3.888592E-5	45.0	12
CGGCTAC	25	3.888592E-5	45.0	25
ATCGGTC	25	3.888592E-5	45.0	26
CATCCGA	20	7.0306147E-4	45.0	16
CCGTTAT	20	7.0306147E-4	45.0	24
AATCGCG	20	7.0306147E-4	45.0	19
CAGACGA	20	7.0306147E-4	45.0	3
TAATACG	130	0.0	43.26923	4
AGTACGG	125	0.0	41.4	2
GCGATAT	60	3.6379788E-12	41.250004	9
CGTTTTT	935	0.0	41.14973	1
ACGGGTC	55	6.002665E-11	40.909092	5
ATAACGG	45	1.925764E-8	40.000004	2
>>END_MODULE