##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346558
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.072169737821664	31.0	31.0	33.0	28.0	34.0
2	31.12555474119772	31.0	31.0	34.0	28.0	34.0
3	30.80421170482286	31.0	30.0	34.0	27.0	34.0
4	34.76888428488161	35.0	35.0	37.0	32.0	37.0
5	35.09683804731098	35.0	35.0	37.0	33.0	37.0
6	35.11900749658066	36.0	35.0	37.0	32.0	37.0
7	31.156986709295413	35.0	35.0	37.0	0.0	37.0
8	33.152742109545876	36.0	35.0	37.0	17.0	37.0
9	36.32035907409438	39.0	35.0	39.0	32.0	39.0
10	36.208444185388885	37.0	35.0	39.0	32.0	39.0
11	36.43758620490654	38.0	35.0	39.0	32.0	39.0
12	36.482008783522524	38.0	35.0	39.0	32.0	39.0
13	36.338029997864716	38.0	35.0	39.0	32.0	39.0
14	37.39495553413859	39.0	36.0	40.0	32.0	41.0
15	37.59153446176398	39.0	36.0	41.0	33.0	41.0
16	37.5294496159373	39.0	36.0	41.0	33.0	41.0
17	37.43181805065819	39.0	36.0	40.0	32.0	41.0
18	37.300786592720414	39.0	36.0	40.0	32.0	41.0
19	37.043787764241486	38.0	36.0	40.0	32.0	41.0
20	36.93870867214146	38.0	35.0	40.0	32.0	41.0
21	36.91483099510039	38.0	35.0	40.0	32.0	41.0
22	36.98621587151357	39.0	35.0	40.0	32.0	41.0
23	37.05118335170447	39.0	35.0	40.0	32.0	41.0
24	36.976477241904675	39.0	35.0	40.0	32.0	41.0
25	36.63942832080056	38.0	35.0	40.0	31.0	41.0
26	36.589719469756865	38.0	35.0	40.0	31.0	41.0
27	36.551769112240954	38.0	35.0	40.0	31.0	41.0
28	36.5777763029565	38.0	35.0	40.0	31.0	41.0
29	36.51593095528021	38.0	35.0	40.0	31.0	41.0
30	36.23057323738018	38.0	35.0	40.0	30.0	41.0
31	36.128766324828746	38.0	35.0	40.0	30.0	41.0
32	35.74821242043179	38.0	35.0	40.0	29.0	41.0
33	35.547847690718434	38.0	35.0	40.0	27.0	41.0
34	35.370076581697724	38.0	35.0	40.0	26.0	41.0
35	35.01163441617276	38.0	34.0	40.0	24.0	41.0
36	34.90933407972114	38.0	34.0	40.0	23.0	41.0
37	34.761985006838685	38.0	34.0	40.0	23.0	41.0
38	34.59449789068496	38.0	34.0	40.0	23.0	41.0
39	34.644723249787916	38.0	34.0	40.0	23.0	41.0
40	34.45746166586834	38.0	34.0	40.0	22.0	41.0
41	34.22082883673151	38.0	33.0	40.0	21.0	41.0
42	34.152040928214035	37.0	33.0	40.0	21.0	41.0
43	34.116719279312555	37.0	33.0	40.0	21.0	41.0
44	33.92180241114041	37.0	33.0	40.0	20.0	41.0
45	33.835158905580016	37.0	33.0	40.0	20.0	41.0
46	33.65348657367598	37.0	33.0	40.0	19.0	41.0
47	33.57639990997178	37.0	33.0	40.0	19.0	41.0
48	33.46321250699739	37.0	32.0	40.0	19.0	41.0
49	33.38875166638773	36.0	32.0	40.0	18.0	41.0
50	33.19595276981054	36.0	32.0	40.0	18.0	41.0
51	31.476024215282866	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	20.0
10	23.0
11	17.0
12	14.0
13	5.0
14	16.0
15	26.0
16	40.0
17	89.0
18	193.0
19	358.0
20	694.0
21	1072.0
22	1672.0
23	2495.0
24	3643.0
25	5359.0
26	6958.0
27	7624.0
28	7868.0
29	8192.0
30	9082.0
31	11002.0
32	13894.0
33	18802.0
34	28807.0
35	31902.0
36	32681.0
37	47277.0
38	61077.0
39	45648.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.62528061680873	19.670877602017555	24.60540515584693	17.098436625326784
2	37.35478621183179	23.804673388004318	23.18082398905811	15.659716411105789
3	30.47195563224626	22.049411642495627	32.71487023817081	14.763762487087298
4	28.10496367130466	24.232884538807355	30.335759093715915	17.326392696172068
5	24.392742340387468	28.629262634248814	29.667472688554298	17.310522336809424
6	25.447976962009243	33.38027112344831	28.27088106464142	12.900870849901027
7	63.60753466952141	18.30833511273726	14.427310868599196	3.656819349142135
8	72.7927215646443	7.500043282798262	14.502334385586252	5.204900766971185
9	66.72678166425244	8.60635160636892	17.220205564436544	7.446661164942088
10	38.80937678541543	25.169235741203494	23.60095568418562	12.420431789195458
11	30.43732939363685	25.320725535119664	28.531154958188814	15.710790113054667
12	29.04073776972397	21.708054640204523	32.579250803617285	16.671956786454214
13	23.0628062257977	26.313344375256094	33.074983119708676	17.54886627923753
14	18.448282827116962	28.7443948776251	32.448536752866765	20.35878554239117
15	17.402570421112774	25.466732841256007	39.48112581443799	17.64957092319323
16	18.66065709058801	24.778536348893983	37.04776689616168	19.513039664356327
17	19.393867693142273	23.574120349263328	34.006140386313405	23.025871571280998
18	20.016562884134835	25.37468475695266	35.19872575441917	19.410026604493332
19	22.5864068929299	25.98035538062893	31.974445835906256	19.45879189053492
20	24.207780515815536	25.20703605168543	33.52685553356148	17.05832789893755
21	22.254572106256383	26.874866544705363	32.92522463772298	17.945336711315278
22	20.28231926546206	24.753143773913745	32.01022628246931	22.954310678154883
23	19.79004957323161	26.736938694244543	32.81874895399904	20.654262778524807
24	20.79334483693927	24.00781398784619	33.992289890869635	21.2065512843449
25	19.074152089982054	26.86505577709936	32.78441126737805	21.276380865540546
26	19.621246660010737	27.459184321239157	32.50018755879246	20.41938145995764
27	20.047726498883303	26.9273830065963	34.0748157595554	18.950074734964996
28	17.925715176103278	26.685287888318836	36.145753380386545	19.243243555191338
29	21.19154658094749	25.383629868593427	33.34045094904749	20.084372601411594
30	21.087667865119258	25.00072137997103	33.67690256753559	20.23470818737412
31	23.307209759982456	26.230241402593506	30.73540359766619	19.72714523975785
32	24.32089289527294	27.196024907807644	30.444254641358732	18.038827555560687
33	22.919395887557062	25.72556397486135	31.189584427426286	20.1654557101553
34	22.269865361642207	25.97458434086069	32.218560818102596	19.536989479394503
35	22.758960982000126	23.72647579914473	32.41852734607194	21.0960358727832
36	23.682615896906146	25.138360678443433	32.49816769487359	18.680855729776834
37	22.30189463235591	26.151178157768683	33.90716705428817	17.639760155587233
38	22.153867462300685	26.732033310441544	30.12396193422169	20.99013729303609
39	24.526341911022108	24.018201859429013	31.43023678576169	20.025219443787186
40	24.40918980372694	24.25337172998459	32.39198056313806	18.945457903150412
41	20.39196902105852	24.915021439412737	32.89925495876592	21.793754580762815
42	21.089110625061316	24.345996918264763	33.06430669613744	21.500585760536474
43	22.878710057191004	23.934521782789606	32.377264411729065	20.80950374829033
44	22.17175768558221	25.061894401514323	31.129854165825055	21.63649374707841
45	22.801955228273478	24.46892006532817	29.397965131377717	23.33115957502063
46	23.35886056590816	26.090005136225393	29.715949422607473	20.835184875258975
47	20.566542974047632	25.073725033039203	34.14810796461198	20.211624028301177
48	20.83691618718945	23.6765563051495	33.82637249753288	21.660155010128175
49	20.762469774179216	22.605451324165074	34.78119102718737	21.85088787446834
50	20.421401323876523	24.31079357567853	32.694960150970395	22.572844949474547
51	20.35041753472723	23.117631103595933	31.09263095931994	25.43932040235689
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	267.0
1	471.5
2	676.0
3	5108.0
4	9540.0
5	6518.5
6	3497.0
7	3174.5
8	2852.0
9	2694.5
10	2537.0
11	2445.0
12	2353.0
13	2215.0
14	2077.0
15	1954.5
16	1832.0
17	1713.5
18	1595.0
19	1565.0
20	1535.0
21	1719.5
22	1904.0
23	1901.5
24	1899.0
25	2520.0
26	3414.5
27	3688.0
28	4689.5
29	5691.0
30	6534.5
31	7378.0
32	7557.5
33	7737.0
34	8520.5
35	9304.0
36	10249.0
37	11194.0
38	11805.0
39	12416.0
40	14417.5
41	16419.0
42	19077.5
43	21736.0
44	23112.0
45	24488.0
46	28644.0
47	32800.0
48	32824.5
49	32849.0
50	30892.0
51	28935.0
52	24449.0
53	19963.0
54	17651.0
55	15339.0
56	13876.5
57	12414.0
58	11711.0
59	11008.0
60	11241.0
61	11474.0
62	10425.0
63	9376.0
64	7680.0
65	5984.0
66	4875.5
67	3767.0
68	3164.5
69	2562.0
70	2135.0
71	1708.0
72	1377.5
73	1047.0
74	908.0
75	563.5
76	358.0
77	274.5
78	191.0
79	164.5
80	138.0
81	105.5
82	73.0
83	47.5
84	22.0
85	19.0
86	16.0
87	10.0
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	346558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.878840912005785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92682700019266	28.369722148054965
2	9.17007245356822	6.763632863334179
3	3.5545020486505847	3.9325774672075062
4	1.8943292230323407	2.7944266420469286
5	1.250076904603071	2.3050693646314646
6	0.8316513545166965	1.8402202798485225
7	0.6050021053140031	1.561824347531258
8	0.46912881598274697	1.384074157749229
9	0.34578889064392515	1.1477064138476651
>10	4.7270408703861335	39.915761837691655
>50	0.18499148424561235	4.235277672489617
>100	0.035124965363090954	2.4606234992483684
>500	0.002341664357539397	0.6508504599673094
>1k	0.002341664357539397	1.005781294615559
>5k	7.805547858464656E-4	1.6324515517357858
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5671	1.6363783262830465	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	1174	0.338760034395397	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG	1174	0.338760034395397	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	1146	0.33068057871986795	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	870	0.2510402299182244	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	852	0.24584629412681283	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	539	0.15552952175393442	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	443	0.12782853086640622	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	437	0.1260972189359357	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	408	0.11772921127199487	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	384	0.11080396355011282	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	377	0.10878409963123055	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	370	0.10676423571234829	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20458335978393227	0.0
2	0.0	0.0	0.0	1.2168237351323588	0.0
3	0.0	0.0	0.0	1.5117238672891695	0.0
4	0.0	0.0	0.0	1.9379151541733275	0.0
5	0.0	0.0	0.0	3.2288967503275066	0.0
6	0.0	0.0	0.0	3.7863791919390115	0.0
7	0.0	0.0	0.0	4.401572031232868	0.0
8	0.0	0.0	0.0	5.464886108530174	0.0
9	0.0	0.0	0.0	5.883575043715626	0.0
10	0.0	0.0	0.0	7.242654909134979	0.0
11	0.0	0.0	0.0	8.619913549824272	0.0
12	0.0	0.0	0.0	10.079698059199327	0.0
13	0.0	0.0	0.0	10.589857974711304	0.0
14	0.0	0.0	0.0	10.796172646425706	0.0
15	0.0	0.0	0.0	11.266512387536862	0.0
16	0.0	0.0	0.0	12.062050219588063	0.0
17	0.0	0.0	0.0	12.963198079397966	0.0
18	0.0	0.0	0.0	13.958990991406921	0.0
19	0.0	0.0	0.0	14.525995648636014	0.0
20	0.0	0.0	0.0	15.075975738548815	0.0
21	0.0	0.0	0.0	15.718869568730199	0.0
22	0.0	0.0	0.0	16.3744596864017	0.0
23	0.0	0.0	0.0	17.060347762856434	0.0
24	0.0	0.0	0.0	17.623889796224585	0.0
25	0.0	0.0	0.0	18.12048776828121	0.0
26	0.0	0.0	0.0	18.567166246342605	0.0
27	0.0	0.0	0.0	18.977198621875704	0.0
28	0.0	0.0	0.0	19.40800674057445	0.0
29	0.0	0.0	0.0	19.86911281805643	0.0
30	0.0	0.0	0.0	20.350706086715643	0.0
31	0.0	0.0	0.0	20.84211012298086	0.0
32	0.0	0.0	0.0	21.311295656138366	0.0
33	0.0	0.0	0.0	21.77297883759717	0.0
34	0.0	0.0	0.0	22.232642155137093	0.0
35	0.0	0.0	0.0	22.73645392690401	0.0
36	0.0	0.0	0.0	23.24517108247393	0.0
37	0.0	0.0	0.0	23.73599801476232	0.0
38	0.0	0.0	0.0	24.18902463656877	0.0
39	0.0	0.0	0.0	24.669175145285926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACA	20	7.0264586E-4	45.000004	16
CCCCTTG	30	2.1610977E-6	45.000004	37
AGATCGG	20	7.0264586E-4	45.000004	1
CCCTCGT	80	0.0	45.000004	14
CCTAGTG	20	7.0264586E-4	45.000004	19
TGGGTAC	25	3.8851475E-5	45.000004	6
CGTTGCG	25	3.8851475E-5	45.000004	31
TCGGGAT	20	7.0264586E-4	45.000004	5
TGAACCG	20	7.0264586E-4	45.000004	1
CGACCCG	25	3.8851475E-5	45.000004	32
TACGGTG	20	7.0264586E-4	45.000004	33
TAAGTAT	25	3.8851475E-5	45.000004	14
CGCGACC	25	3.8851475E-5	45.000004	10
TCGATCA	20	7.0264586E-4	45.000004	17
TCGATAG	25	3.8851475E-5	45.000004	1
CCTCGAT	20	7.0264586E-4	45.000004	15
GTTACGA	20	7.0264586E-4	45.000004	25
AAACGGC	25	3.8851475E-5	45.000004	6
CTACGAC	20	7.0264586E-4	45.000004	26
TACGACC	20	7.0264586E-4	45.000004	27
>>END_MODULE