##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506037
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.128561745485015	31.0	31.0	33.0	28.0	34.0
2	31.16303155698101	31.0	31.0	34.0	28.0	34.0
3	30.97593061376935	31.0	31.0	34.0	28.0	34.0
4	34.938838464381064	35.0	35.0	37.0	32.0	37.0
5	35.133717494965786	35.0	35.0	37.0	33.0	37.0
6	35.08640277291977	36.0	35.0	37.0	32.0	37.0
7	31.05047457004132	35.0	35.0	37.0	0.0	37.0
8	32.94234611303126	35.0	35.0	37.0	17.0	37.0
9	36.03579975377295	38.0	34.0	39.0	32.0	39.0
10	36.01276784108672	37.0	35.0	39.0	32.0	39.0
11	36.250793914278994	37.0	35.0	39.0	32.0	39.0
12	36.35228056446466	38.0	35.0	39.0	32.0	39.0
13	36.209998478372135	38.0	35.0	39.0	32.0	39.0
14	37.375543685540784	39.0	36.0	40.0	32.0	41.0
15	37.42621982187073	39.0	36.0	41.0	32.0	41.0
16	37.5357869088624	39.0	36.0	41.0	33.0	41.0
17	37.28389030841618	39.0	36.0	40.0	32.0	41.0
18	37.25595361603993	39.0	36.0	40.0	32.0	41.0
19	37.0729215452625	39.0	36.0	40.0	31.0	41.0
20	36.95161025774795	39.0	35.0	40.0	31.0	41.0
21	37.14207459138363	39.0	35.0	40.0	32.0	41.0
22	37.19629592302539	39.0	35.0	40.0	32.0	41.0
23	37.26072006592403	39.0	35.0	40.0	32.0	41.0
24	37.165566944709575	39.0	35.0	40.0	32.0	41.0
25	36.962726045723926	39.0	35.0	40.0	31.0	41.0
26	36.84143056733006	39.0	35.0	40.0	31.0	41.0
27	36.87729948600597	39.0	35.0	40.0	31.0	41.0
28	36.8376482352081	39.0	35.0	40.0	31.0	41.0
29	36.787462181619134	39.0	35.0	40.0	31.0	41.0
30	36.624681594428864	39.0	35.0	40.0	30.0	41.0
31	36.605716578036784	39.0	35.0	40.0	30.0	41.0
32	36.45809496143563	39.0	35.0	40.0	30.0	41.0
33	36.32243294462658	39.0	35.0	40.0	30.0	41.0
34	36.28004276367143	39.0	35.0	40.0	30.0	41.0
35	36.25207445305383	39.0	35.0	40.0	30.0	41.0
36	36.09593764882805	39.0	35.0	40.0	30.0	41.0
37	36.09839201481314	39.0	35.0	40.0	30.0	41.0
38	35.86936923584639	38.0	35.0	40.0	29.0	41.0
39	35.8796076176248	38.0	35.0	40.0	29.0	41.0
40	35.76927971670056	38.0	35.0	40.0	28.0	41.0
41	35.590146570310075	38.0	35.0	40.0	27.0	41.0
42	35.626825706420675	38.0	35.0	40.0	28.0	41.0
43	35.60933884281189	38.0	35.0	40.0	28.0	41.0
44	35.48121184814549	38.0	34.0	40.0	27.0	41.0
45	35.401109009815485	38.0	34.0	40.0	27.0	41.0
46	35.239782861727505	38.0	34.0	40.0	26.0	41.0
47	35.08561231688592	38.0	34.0	40.0	26.0	41.0
48	35.038712584257674	38.0	34.0	40.0	26.0	41.0
49	34.95046607263896	38.0	34.0	40.0	26.0	41.0
50	34.81917132541692	37.0	34.0	40.0	26.0	41.0
51	33.100907641140864	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	33.0
10	38.0
11	32.0
12	15.0
13	18.0
14	29.0
15	31.0
16	57.0
17	145.0
18	366.0
19	583.0
20	1013.0
21	1389.0
22	2005.0
23	2743.0
24	3730.0
25	4816.0
26	6141.0
27	7152.0
28	7947.0
29	9439.0
30	11716.0
31	14731.0
32	19042.0
33	26836.0
34	40023.0
35	43460.0
36	49382.0
37	73595.0
38	98757.0
39	80761.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.56154194258523	19.598566903210635	21.86519958026785	10.974691573936294
2	31.274985821194896	30.276442236437255	22.737072585601446	15.711499356766403
3	31.56587364165071	29.119609830901695	25.072079709586454	14.242436817861146
4	27.68473451546033	23.59946011852888	31.38821864804352	17.327586717967264
5	29.99326136231145	27.891043540294486	23.273989846592244	18.84170525080182
6	25.313168799909885	38.48117825376406	23.747275396858335	12.458377549467727
7	65.30530376237311	17.60879145200845	13.549799718202424	3.536105067416019
8	74.10801976930541	11.578995211812575	10.019227843023335	4.2937571758586826
9	68.48669168460015	8.221137980029129	10.867980009366903	12.42419032600383
10	42.19296217470264	25.6299440554742	18.341939423401847	13.835154346421309
11	33.203303315765446	24.891262891843876	24.67566600861202	17.22976778377866
12	31.10523538792618	21.05221554945587	27.582765687094025	20.259783375523924
13	25.054887290850274	23.558356404768823	32.1002219205315	19.286534383849403
14	20.979295980333454	28.7188486217411	27.317370073729784	22.98448532419566
15	18.57018360317526	26.706347559565803	35.6209130952875	19.102555741971436
16	21.431239217685665	25.860757217357623	30.03555076012228	22.67245280483443
17	21.220582684665352	25.258824947582887	31.629900580392345	21.890691787359422
18	21.527674853815036	26.868588660512966	29.184427225677172	22.419309259994822
19	23.76960577981452	28.444362763987613	25.9965180411709	21.789513415026963
20	26.49450534249472	26.283651195465946	30.04365293446922	17.17819052757012
21	25.24222537087209	30.088511314390054	26.26072797048437	18.408535344253483
22	22.99318034056798	24.2310344895729	28.728334094147268	24.047451075711855
23	22.940417400308675	29.804737598238862	28.19991423552033	19.054930765932134
24	24.282414131772974	24.998962526455575	27.469928088262318	23.24869525350913
25	21.282831097330828	31.403830154712008	25.94553362698775	21.367805120969415
26	22.028823979274243	26.99091173175084	28.30148783586971	22.678776453105208
27	24.19072123184668	29.29291731632272	25.78368775405751	20.732673697773087
28	20.133903252133738	28.339627339502844	31.149500925821627	20.37696848254179
29	24.970110881220148	26.543513616593252	27.38475645061527	21.101619051571326
30	23.707554981157504	28.321051622707433	28.553643310667002	19.417750085468057
31	25.27759827838676	25.85502641111223	26.819580386414433	22.047794924086578
32	25.57085746694412	31.50105624687523	24.992441264176335	17.93564502200432
33	23.679493791955924	28.243982159407317	25.13096868410808	22.94555536452868
34	26.829263472829062	26.999606748123163	26.97668352314159	19.19444625590619
35	23.995280977477933	28.111580773737888	25.17602467803738	22.717113570746804
36	24.08460250930268	28.66272624333794	26.013512845898617	21.239158401460763
37	24.26502409902833	30.207672561492537	26.86700774844527	18.660295591033858
38	21.162879394194494	30.56080879461383	25.65642433260809	22.619887478583582
39	25.543389119767923	28.02364253997237	25.74495540839899	20.688012931860715
40	22.940812628325595	29.412276177433665	27.876617717676773	19.770293476563968
41	22.05451380037428	27.154733744765696	27.026087025257045	23.764665429602974
42	20.485656187195797	29.213871712937987	29.07435622296393	21.22611587690228
43	23.132498216533573	26.233852465333563	25.51928021073558	25.114369107397284
44	23.202651189537523	26.461701417090055	27.731569035465785	22.604078357906634
45	22.116564599031296	25.375021984558444	27.040315233866295	25.46809818254396
46	24.413036991366244	29.289755492187332	24.587727774846506	21.70947974159992
47	20.664694478862216	26.477115309750076	31.932052399330484	20.92613781205722
48	23.048512262937297	27.145050658351067	26.869379116546817	22.937057962164822
49	20.667461074980682	25.58864272770568	30.716923861298678	23.026972336014957
50	20.31985803409632	25.796927892624456	29.196481680193347	24.68673239308588
51	20.797095864531645	25.448929623723167	26.456563452870046	27.29741105887514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	510.0
2	897.0
3	2651.0
4	4405.0
5	3073.5
6	1742.0
7	1605.0
8	1468.0
9	1428.0
10	1388.0
11	1338.5
12	1289.0
13	1271.5
14	1254.0
15	1211.0
16	1168.0
17	1346.5
18	1525.0
19	1576.5
20	1628.0
21	1817.0
22	2006.0
23	2355.0
24	2704.0
25	3213.5
26	4557.5
27	5392.0
28	6635.5
29	7879.0
30	8621.5
31	9364.0
32	10853.5
33	12343.0
34	14099.0
35	15855.0
36	16537.5
37	17220.0
38	18868.0
39	20516.0
40	21926.5
41	23337.0
42	25479.0
43	27621.0
44	29856.5
45	32092.0
46	48606.5
47	65121.0
48	51859.5
49	38598.0
50	36988.0
51	35378.0
52	31443.5
53	27509.0
54	25928.0
55	24347.0
56	24111.5
57	23876.0
58	22898.5
59	21921.0
60	22235.5
61	22550.0
62	20953.0
63	19356.0
64	16179.5
65	13003.0
66	9759.5
67	6516.0
68	5403.5
69	4291.0
70	3567.5
71	2844.0
72	2249.0
73	1654.0
74	1333.0
75	773.5
76	535.0
77	392.5
78	250.0
79	211.5
80	173.0
81	119.5
82	66.0
83	60.5
84	55.0
85	41.0
86	27.0
87	18.5
88	10.0
89	5.5
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	506037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.187700541805718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.95107381486446	21.29274096711428
2	8.439392041874079	4.926528953462379
3	3.6057218338027837	3.1572818736625865
4	2.2293683106897704	2.6028053859921725
5	1.5473019678459212	2.2581093242616723
6	1.1224396393149954	1.9656859241147104
7	0.9132529738907951	1.8659028024586632
8	0.7242419993293698	1.6911166876979447
9	0.6337124700284676	1.6646948824319048
>10	7.654632647080588	47.105480953349186
>50	0.1334692172421204	2.3698265979815267
>100	0.0372629794330793	2.2171642036522763
>500	0.002032526150895235	0.42001933362089144
>1k	0.00406505230179047	1.804659340218576
>5k	0.002032526150895235	4.6579827699812135
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	8593	1.698097174712521	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	8538	1.6872284042471202	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	6424	1.2694723903588077	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2301	0.45470983347067506	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	2070	0.4090609975159919	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	1551	0.30649932712430117	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	1115	0.22033961943494249	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	1058	0.20907562095261809	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	1031	0.2037400427241486	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	785	0.15512699664253798	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	686	0.13556320980481665	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	653	0.12904194752557618	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09090244389244265	0.0
2	0.0	0.0	0.0	0.7256386390718466	0.0
3	0.0	0.0	0.0	0.8469736402674113	0.0
4	0.0	0.0	0.0	1.232716184784907	0.0
5	0.0	0.0	0.0	3.063609973183779	0.0
6	0.0	0.0	0.0	3.4072607338989047	0.0
7	0.0	0.0	0.0	3.966310763837427	0.0
8	0.0	0.0	0.0	4.587806820449888	0.0
9	0.0	0.0	0.0	4.735226870762415	0.0
10	0.0	0.0	0.0	7.043951331621996	0.0
11	0.0	0.0	0.0	7.57948529455356	0.0
12	0.0	0.0	0.0	10.001047354244848	0.0
13	0.0	0.0	0.0	10.270592861786787	0.0
14	0.0	0.0	0.0	10.404970387540832	0.0
15	0.0	0.0	0.0	10.813438543031438	0.0
16	0.0	0.0	0.0	11.200366771599706	0.0
17	0.0	0.0	0.0	11.578007141770266	0.0
18	0.0	0.0	0.0	11.963947300296223	0.0
19	0.0	0.0	0.0	12.333090268102925	0.0
20	0.0	0.0	0.0	12.609552265941028	0.0
21	0.0	0.0	0.0	12.935220151886126	0.0
22	0.0	0.0	0.0	13.282625578762028	0.0
23	0.0	0.0	0.0	13.608293464707126	0.0
24	0.0	0.0	0.0	13.86756304380905	0.0
25	0.0	0.0	0.0	14.109442590166331	0.0
26	0.0	0.0	0.0	14.340453366058213	0.0
27	0.0	0.0	0.0	14.5916207708132	0.0
28	0.0	0.0	0.0	14.833697931178945	0.0
29	0.0	0.0	0.0	15.078739301671616	0.0
30	0.0	0.0	0.0	15.363303473856655	0.0
31	0.0	0.0	0.0	15.639567857686295	0.0
32	0.0	0.0	0.0	15.915634627507474	0.0
33	0.0	0.0	0.0	16.162849752093226	0.0
34	0.0	0.0	0.0	16.430023891533622	0.0
35	0.0	0.0	0.0	16.713797607684814	0.0
36	0.0	0.0	0.0	17.01219476046218	0.0
37	0.0	0.0	0.0	17.295178020579524	0.0
38	0.0	0.0	0.0	17.583694472933797	0.0
39	0.0	0.0	0.0	17.882289239719626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	30	2.163084E-6	45.000004	2
CGACGGT	20	7.029539E-4	45.0	28
CGTTCCA	20	7.029539E-4	45.0	22
GCGCACA	20	7.029539E-4	45.0	33
TCGGCAC	20	7.029539E-4	45.0	28
TCGTGCG	35	1.2101191E-7	45.0	1
GTATGCG	25	3.8877006E-5	45.0	1
CATCGCG	20	7.029539E-4	45.0	30
ACGGTCT	20	7.029539E-4	45.0	30
ACGGGAT	150	0.0	43.5	5
ACGGCTG	1125	0.0	42.6	8
TACGGCT	1015	0.0	42.3399	7
CGGCTGT	1125	0.0	42.0	9
CGGGACC	70	0.0	41.785713	6
TGATACC	930	0.0	41.612904	4
CGAATAT	115	0.0	41.086956	14
GATACCT	940	0.0	40.93085	5
TATGCGG	55	6.002665E-11	40.909092	2
CGTTAGG	55	6.002665E-11	40.909092	2
TCGTTGA	50	1.0786607E-9	40.5	24
>>END_MODULE