##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439583
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16120505115075	31.0	31.0	33.0	28.0	34.0
2	31.228937424786672	31.0	31.0	34.0	28.0	34.0
3	30.995438859100556	31.0	31.0	34.0	28.0	34.0
4	34.968715805661276	35.0	35.0	37.0	32.0	37.0
5	35.19766460486416	35.0	35.0	37.0	33.0	37.0
6	35.16471974575905	36.0	35.0	37.0	32.0	37.0
7	31.208326982617617	35.0	35.0	37.0	0.0	37.0
8	33.171307807626775	36.0	35.0	37.0	17.0	37.0
9	36.41923595771447	39.0	35.0	39.0	32.0	39.0
10	36.16086381866451	37.0	35.0	39.0	32.0	39.0
11	36.36659060973695	38.0	35.0	39.0	32.0	39.0
12	36.3411915383443	38.0	35.0	39.0	32.0	39.0
13	36.20956906886754	38.0	35.0	39.0	32.0	39.0
14	37.26417991596581	39.0	36.0	40.0	32.0	41.0
15	37.47581002905026	39.0	36.0	41.0	32.0	41.0
16	37.45966290780126	39.0	36.0	41.0	33.0	41.0
17	37.33000138767878	39.0	35.0	40.0	32.0	41.0
18	37.26301745062935	39.0	36.0	40.0	32.0	41.0
19	37.06697938728295	39.0	35.0	40.0	32.0	41.0
20	37.02964627840476	39.0	35.0	40.0	32.0	41.0
21	37.04256534033391	39.0	35.0	40.0	32.0	41.0
22	37.124745497437345	39.0	35.0	40.0	32.0	41.0
23	37.20323579392288	39.0	35.0	40.0	32.0	41.0
24	37.10416917851691	39.0	35.0	40.0	32.0	41.0
25	36.779163434436725	38.0	35.0	40.0	31.0	41.0
26	36.73518994137626	38.0	35.0	40.0	31.0	41.0
27	36.76342579217122	39.0	35.0	40.0	31.0	41.0
28	36.76483621978102	39.0	35.0	40.0	31.0	41.0
29	36.764699726786525	39.0	35.0	40.0	31.0	41.0
30	36.568331805370086	38.0	35.0	40.0	31.0	41.0
31	36.5063025640209	38.0	35.0	40.0	31.0	41.0
32	36.32011701999395	38.0	35.0	40.0	30.0	41.0
33	36.173175486768145	38.0	35.0	40.0	30.0	41.0
34	36.05810961752388	38.0	35.0	40.0	30.0	41.0
35	36.02054902032153	38.0	35.0	40.0	30.0	41.0
36	35.946995220470306	38.0	35.0	40.0	30.0	41.0
37	35.864482930413594	38.0	35.0	40.0	29.0	41.0
38	35.754958676745915	38.0	35.0	40.0	29.0	41.0
39	35.737717336657695	38.0	35.0	40.0	29.0	41.0
40	35.596381115739234	38.0	35.0	40.0	28.0	41.0
41	35.46704262903707	38.0	34.0	40.0	27.0	41.0
42	35.44804052931983	38.0	34.0	40.0	28.0	41.0
43	35.37661374529952	38.0	34.0	40.0	27.0	41.0
44	35.19245967200733	38.0	34.0	40.0	27.0	41.0
45	35.12929298903734	38.0	34.0	40.0	26.0	41.0
46	35.035654245045876	38.0	34.0	40.0	26.0	41.0
47	34.957448308965546	37.0	34.0	40.0	26.0	41.0
48	34.88979100647659	37.0	34.0	40.0	26.0	41.0
49	34.7934724500265	37.0	34.0	40.0	26.0	41.0
50	34.621009001712984	37.0	34.0	40.0	25.0	41.0
51	32.98353212021393	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	39.0
10	40.0
11	38.0
12	28.0
13	19.0
14	28.0
15	41.0
16	69.0
17	114.0
18	246.0
19	406.0
20	656.0
21	987.0
22	1545.0
23	2239.0
24	3067.0
25	4221.0
26	5580.0
27	6701.0
28	7293.0
29	8254.0
30	10193.0
31	12700.0
32	17079.0
33	23838.0
34	38438.0
35	40799.0
36	42470.0
37	62837.0
38	83738.0
39	65861.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15430760516217	20.946442423842594	23.535259552803453	12.363990418191786
2	33.56726716001302	24.066672278045328	24.595582631721427	17.77047793022023
3	32.0246688338721	24.25753498201705	28.293860317619195	15.423935866491654
4	29.45109342263008	24.66451159394244	27.741291178230277	18.143103805197196
5	25.818787350739225	28.984059893125984	25.943678440704034	19.25347431543076
6	26.3811384880671	35.74819772375183	25.321952850769936	12.548710937411137
7	69.14007138583612	17.106667000316207	10.180557482887192	3.572704130960479
8	79.67414572447069	6.6492562269241535	9.696689817395123	3.9799082312100333
9	74.3588810304311	7.16133244461228	11.086188501375167	7.39359802358144
10	44.52833708309921	23.797553590561964	17.7873120662082	13.886797260130624
11	34.81617805966109	24.13651119356299	23.446311618056203	17.600999128719717
12	31.373369761796976	22.129381709483763	27.855717805283643	18.641530723435622
13	25.34424670653779	24.52096646139637	30.742089662248084	19.39269716981776
14	20.446650575659202	28.298637572426593	29.728401689783272	21.52631016213093
15	20.10405315947159	25.10242661795383	35.737278284192065	19.056241938382513
16	22.85461448691146	23.79823605553445	33.12275497460092	20.224394482953162
17	22.37461412293014	23.698141192903275	30.29712250018768	23.63012218397891
18	24.38834076841006	25.298066576732946	29.333481959038455	20.98011069581854
19	25.374730141975466	27.069745645304756	27.27266523045705	20.282858982262734
20	28.080703757879625	25.676379659813957	28.11505449482805	18.12786208747836
21	25.76782996612699	26.95327162333393	27.155053766865418	20.123844643673664
22	23.146936983459323	24.482065957964707	29.196988964541394	23.174008094034573
23	23.540491784259174	26.780607985295156	28.316381661711215	21.36251856873446
24	24.61901392911009	23.47952491338382	29.637406360118568	22.264054797387526
25	23.000207014374986	26.18731843588128	27.83183153124666	22.980643018497076
26	21.517893094136944	27.4728549557194	28.714713717318457	22.2945382328252
27	22.539088181299093	27.233764727025385	28.845974480359793	21.381172611315723
28	20.143636127875737	27.605480648705704	31.667512164938135	20.583371058480424
29	22.36551458996367	27.361613165204297	30.1321934651704	20.140678779661634
30	23.028188078246885	24.965251158484293	30.177918618326917	21.828642144941913
31	26.261707117882178	25.68274933289049	26.658219266896126	21.39732428233121
32	25.547166291690075	28.663528844382064	26.107015057452177	19.682289806475683
33	25.47641742287577	26.398427600703396	25.9247969097986	22.20035806662223
34	22.782955664800504	27.366162931687533	28.962221014006456	20.88866038950551
35	22.851429650373195	27.542693871237056	26.8588639688068	22.747012509582945
36	24.012757545218992	29.424249800378995	26.944627067015787	19.618365587386226
37	25.323317780714905	28.788874910995194	27.00855128610524	18.879256022184663
38	22.67285131590621	28.62599327089537	25.35994340090495	23.341212012293468
39	25.158843722345953	26.448702520343144	26.454389728447186	21.938064028863717
40	23.32938261943706	26.206199966786702	27.29268420298328	23.171733210792954
41	21.932831797408	27.466257794318704	27.63550910749506	22.965401300778236
42	23.270463143479162	27.062920995579905	27.122522936510286	22.544092924430654
43	24.575563659195193	25.40680599568227	26.24168814535594	23.775942199766597
44	22.670121456016272	26.536967990117905	27.108873637060576	23.684036916805244
45	22.502917537757376	26.66663633489011	26.500569858252028	24.329876269100488
46	23.75410332064707	27.848438178910467	26.29856022639638	22.098898274046082
47	22.36164728845292	26.557441939292463	29.319832659588744	21.761078112665867
48	23.077098067941662	25.18068260146548	28.437632938489433	23.304586392103424
49	22.148718217037512	24.826255792421453	29.523434709713527	23.501591280827512
50	20.609532215759028	26.486010605505673	28.7251781802299	24.179278998505403
51	20.232811550947147	26.21484452310485	27.77655186847535	25.775792057472653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	230.0
1	424.5
2	619.0
3	2982.0
4	5345.0
5	3695.0
6	2045.0
7	1938.5
8	1832.0
9	1709.0
10	1586.0
11	1528.5
12	1471.0
13	1443.5
14	1416.0
15	1315.5
16	1215.0
17	1371.5
18	1528.0
19	1525.0
20	1522.0
21	1651.0
22	1780.0
23	1734.5
24	1689.0
25	1983.0
26	2841.0
27	3405.0
28	4338.5
29	5272.0
30	6199.0
31	7126.0
32	7691.5
33	8257.0
34	9127.0
35	9997.0
36	11297.0
37	12597.0
38	14489.5
39	16382.0
40	18229.0
41	20076.0
42	23104.0
43	26132.0
44	28136.0
45	30140.0
46	34694.5
47	39249.0
48	37090.5
49	34932.0
50	34164.5
51	33397.0
52	30905.0
53	28413.0
54	26829.0
55	25245.0
56	23469.5
57	21694.0
58	21551.5
59	21409.0
60	21600.5
61	21792.0
62	19753.5
63	17715.0
64	14841.5
65	11968.0
66	9731.5
67	7495.0
68	5892.5
69	4290.0
70	3810.5
71	3331.0
72	2656.0
73	1981.0
74	1625.0
75	1031.5
76	794.0
77	585.0
78	376.0
79	242.0
80	108.0
81	100.0
82	92.0
83	60.0
84	28.0
85	27.5
86	27.0
87	20.5
88	14.0
89	12.5
90	11.0
91	6.0
92	1.0
93	4.5
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	439583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.38046368751821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34172802599784	21.841944407351843
2	8.507070432976217	4.998833478864351
3	3.442937266966502	3.0346528005153752
4	2.035224605043081	2.3918337041764732
5	1.4123926848163626	2.0748375994381747
6	1.0414217815253985	1.8358472901298495
7	0.7644565120115061	1.5722060754288618
8	0.6048262520930194	1.4216060589501354
9	0.4917415980364087	1.3002836548275796
>10	6.950100389385974	48.524746037443535
>50	0.36628370662779586	6.489031074679589
>100	0.037170439573222414	2.097712790874998
>500	0.0015487683155509338	0.3797269683265045
>1k	0.0030975366311018675	2.0367380589927313
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2682	0.6101236854018467	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGC	2335	0.5311852369177152	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCG	2260	0.514123612605583	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTC	1675	0.38104294297095204	No Hit
GCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	995	0.2263508825409536	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	674	0.15332713048502786	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	440	0.10009486263117547	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.549766483235248E-4	0.0	0.0	0.07734603021499921	0.0
2	4.549766483235248E-4	0.0	0.0	0.5202657973579506	0.0
3	4.549766483235248E-4	0.0	0.0	0.636739819328773	0.0
4	4.549766483235248E-4	0.0	0.0	0.8867494875825498	0.0
5	4.549766483235248E-4	0.0	0.0	1.7787312066208203	0.0
6	4.549766483235248E-4	0.0	0.0	2.0701437498720376	0.0
7	4.549766483235248E-4	0.0	0.0	2.4259354888610343	0.0
8	4.549766483235248E-4	0.0	0.0	3.0365141509112044	0.0
9	4.549766483235248E-4	0.0	0.0	3.230106714772864	0.0
10	4.549766483235248E-4	0.0	0.0	4.271548262785412	0.0
11	4.549766483235248E-4	0.0	0.0	4.956970583484803	0.0
12	4.549766483235248E-4	0.0	0.0	6.080307928195586	0.0
13	4.549766483235248E-4	0.0	0.0	6.3455593141682005	0.0
14	4.549766483235248E-4	0.0	0.0	6.460895894518214	0.0
15	4.549766483235248E-4	0.0	0.0	6.721825002331755	0.0
16	4.549766483235248E-4	0.0	0.0	7.137673658899457	0.0
17	4.549766483235248E-4	0.0	0.0	7.582640820959864	0.0
18	4.549766483235248E-4	0.0	0.0	8.111096197987639	0.0
19	4.549766483235248E-4	0.0	0.0	8.439589338077223	0.0
20	4.549766483235248E-4	0.0	0.0	8.756935550282881	0.0
21	4.549766483235248E-4	0.0	0.0	9.137751004929672	0.0
22	4.549766483235248E-4	0.0	0.0	9.538585432102698	0.0
23	4.549766483235248E-4	0.0	0.0	9.948746880566356	0.0
24	4.549766483235248E-4	0.0	0.0	10.274282672441837	0.0
25	4.549766483235248E-4	0.0	0.0	10.556823171050747	0.0
26	4.549766483235248E-4	0.0	0.0	10.820027162105905	0.0
27	4.549766483235248E-4	0.0	0.0	11.088690872940946	0.0
28	4.549766483235248E-4	0.0	0.0	11.386245600944532	0.0
29	4.549766483235248E-4	0.0	0.0	11.660369031559455	0.0
30	4.549766483235248E-4	0.0	0.0	11.989089659973201	0.0
31	4.549766483235248E-4	0.0	0.0	12.309620708717125	0.0
32	4.549766483235248E-4	0.0	0.0	12.622189666115387	0.0
33	4.549766483235248E-4	0.0	0.0	12.930891322002898	0.0
34	4.549766483235248E-4	0.0	0.0	13.248010045884396	0.0
35	4.549766483235248E-4	0.0	0.0	13.582190394078024	0.0
36	4.549766483235248E-4	0.0	0.0	13.902266466173623	0.0
37	4.549766483235248E-4	0.0	0.0	14.219612678379281	0.0
38	4.549766483235248E-4	0.0	0.0	14.556522886462853	0.0
39	4.549766483235248E-4	0.0	0.0	14.923689041659937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGC	30	2.162431E-6	45.000004	19
CGACGGT	30	2.162431E-6	45.000004	28
ACGCATG	20	7.028527E-4	45.000004	1
TAGCGAA	20	7.028527E-4	45.000004	1
AGACGTC	20	7.028527E-4	45.000004	28
GTTAACG	20	7.028527E-4	45.000004	1
TTGACGT	20	7.028527E-4	45.000004	1
CGAATTG	20	7.028527E-4	45.000004	31
CAAATCG	20	7.028527E-4	45.000004	26
AATTCCG	30	2.162431E-6	45.000004	11
ACGCTCC	30	2.162431E-6	45.000004	22
TTAACGG	30	2.162431E-6	45.000004	2
TATAGCG	20	7.028527E-4	45.000004	1
TCATATG	20	7.028527E-4	45.000004	36
CCGCGGA	20	7.028527E-4	45.000004	30
ATTGCGC	20	7.028527E-4	45.000004	44
TCGACGT	20	7.028527E-4	45.000004	26
TTCGACG	20	7.028527E-4	45.000004	25
TCGAATT	20	7.028527E-4	45.000004	30
AATCGAA	20	7.028527E-4	45.000004	28
>>END_MODULE