##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519640
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15109883765684	31.0	31.0	33.0	28.0	34.0
2	31.19882803479332	31.0	31.0	34.0	28.0	34.0
3	30.960976830113154	31.0	31.0	34.0	27.0	34.0
4	34.932770764375334	35.0	35.0	37.0	32.0	37.0
5	35.18199330305596	35.0	35.0	37.0	33.0	37.0
6	35.166905934877995	36.0	35.0	37.0	32.0	37.0
7	31.034766376722345	35.0	35.0	37.0	0.0	37.0
8	33.09083596335925	36.0	35.0	37.0	17.0	37.0
9	36.37889307982449	39.0	35.0	39.0	32.0	39.0
10	36.14658032484027	37.0	35.0	39.0	32.0	39.0
11	36.37442075282888	38.0	35.0	39.0	32.0	39.0
12	36.38767800785159	38.0	35.0	39.0	32.0	39.0
13	36.31842044492341	38.0	35.0	39.0	32.0	39.0
14	37.37485759371873	39.0	36.0	40.0	32.0	41.0
15	37.60424524670926	39.0	36.0	41.0	33.0	41.0
16	37.5141905934878	39.0	36.0	41.0	33.0	41.0
17	37.41130397967824	39.0	36.0	40.0	32.0	41.0
18	37.33771649603572	39.0	36.0	40.0	32.0	41.0
19	37.14407089523516	39.0	36.0	40.0	32.0	41.0
20	37.1489127087984	39.0	35.0	40.0	32.0	41.0
21	37.12552921253175	39.0	35.0	40.0	32.0	41.0
22	37.194519282580245	39.0	35.0	40.0	32.0	41.0
23	37.26398660611192	39.0	35.0	40.0	32.0	41.0
24	37.19090716649988	39.0	35.0	40.0	32.0	41.0
25	36.87832537910862	39.0	35.0	40.0	31.0	41.0
26	36.86082480178585	39.0	35.0	40.0	31.0	41.0
27	36.89286813948118	39.0	35.0	40.0	31.0	41.0
28	36.910326379801404	39.0	35.0	40.0	31.0	41.0
29	36.92200369486567	39.0	35.0	40.0	31.0	41.0
30	36.68622315449157	39.0	35.0	40.0	31.0	41.0
31	36.650192440920634	39.0	35.0	40.0	31.0	41.0
32	36.525246324378415	39.0	35.0	40.0	31.0	41.0
33	36.40605804018166	39.0	35.0	40.0	30.0	41.0
34	36.316932876606884	39.0	35.0	40.0	30.0	41.0
35	36.251195058117155	39.0	35.0	40.0	30.0	41.0
36	36.2160630436456	39.0	35.0	40.0	30.0	41.0
37	36.15082942036795	38.0	35.0	40.0	30.0	41.0
38	36.031544915710874	38.0	35.0	40.0	30.0	41.0
39	35.99065891771226	38.0	35.0	40.0	30.0	41.0
40	35.880082364714035	38.0	35.0	40.0	29.0	41.0
41	35.76035524593949	38.0	35.0	40.0	29.0	41.0
42	35.76848010160881	38.0	35.0	40.0	29.0	41.0
43	35.7296493726426	38.0	35.0	40.0	29.0	41.0
44	35.53516857824648	38.0	34.0	40.0	28.0	41.0
45	35.44572011392503	38.0	34.0	40.0	28.0	41.0
46	35.339103995073515	38.0	34.0	40.0	27.0	41.0
47	35.29512932029867	38.0	34.0	40.0	27.0	41.0
48	35.23846316680779	38.0	34.0	40.0	27.0	41.0
49	35.14340504964976	37.0	34.0	40.0	27.0	41.0
50	35.00934108228774	37.0	34.0	40.0	27.0	41.0
51	33.37695904857209	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	45.0
10	60.0
11	39.0
12	34.0
13	27.0
14	31.0
15	36.0
16	73.0
17	129.0
18	269.0
19	460.0
20	791.0
21	1121.0
22	1625.0
23	2320.0
24	3393.0
25	4616.0
26	5910.0
27	7031.0
28	7782.0
29	9251.0
30	11438.0
31	14572.0
32	19046.0
33	27170.0
34	42741.0
35	47402.0
36	52431.0
37	77152.0
38	101147.0
39	81473.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.731390962974366	21.896505272881225	23.809560464937267	12.562543299207144
2	33.31440997613733	24.322800400277114	26.00819798321915	16.354591640366408
3	32.729389577399736	23.882110692017548	27.882187668385804	15.506312062196907
4	29.407474405357554	24.79562774228312	27.176314371487955	18.62058348087137
5	26.350357940112385	30.01019936879378	25.202640289431145	18.43680240166269
6	26.262027557539835	35.492456315911014	24.389962281579557	13.855553844969595
7	69.05877145716265	18.278615964898776	9.226387499037795	3.4362250789007773
8	80.41394042029097	6.474482333923485	9.190593487799244	3.9209837579862983
9	73.73835732430145	8.739511969825262	11.126356708490494	6.395773997382803
10	41.95423754907244	25.807482102994385	18.501462550996845	13.73681779693634
11	33.24859518127934	25.669309521976754	24.0610807482103	17.0210145485336
12	30.30578862289277	22.53098298822262	28.558425063505506	18.60480332537911
13	24.800053883457778	25.306173504734048	30.075051959048572	19.818720652759602
14	19.876452928950812	29.098606727734584	29.803710260949888	21.221230082364713
15	20.485528442768068	25.837310445693173	33.96967131090755	19.707489800631205
16	23.147178816103455	25.297321222384728	31.449080132399352	20.10641982911246
17	22.556577630667384	23.954083596335927	29.86856285120468	23.62077592179201
18	23.848241089985375	25.8276883996613	29.046455238241858	21.27761527211146
19	25.045800939111697	26.935185897929337	27.460164729428065	20.558848433530905
20	27.513085982603343	25.756100377184204	27.36048033253791	19.370333307674546
21	25.250942960511125	27.258486644600104	27.83099838349627	19.659572011392505
22	22.988030174736355	25.875606188900008	28.21761219305673	22.918751443306903
23	23.28054037410515	26.72196135786314	28.38984681702717	21.607651451004543
24	23.859210222461705	24.126895543068276	30.428950812100688	21.58494342236933
25	22.657993995843277	26.496997921638055	28.66080363328458	22.184204449234084
26	21.573204526210453	28.154684012008314	27.91701947502117	22.355091986760065
27	21.808367331229313	27.548495112000616	28.63540143176045	22.00773612500962
28	19.40420290970672	28.024593949657454	30.970864444615504	21.600338696020323
29	21.84050496497575	27.355091986760065	28.975252097606035	21.829150950658146
30	21.988492032945885	26.46447540605034	29.42171503348472	22.12531752751905
31	23.648872296205063	26.75583095989531	27.718035563082132	21.877261180817488
32	24.5369871449465	28.690439535062733	26.247594488492034	20.52497883149873
33	23.64848741436379	27.204025864059734	26.242206142714185	22.90528057886229
34	21.239896851666536	28.1541066892464	28.193749518897697	22.412246940189362
35	21.452351628050188	27.584481564159802	27.29735971056886	23.665807097221155
36	22.234623970441074	29.35436071126164	26.20833654068201	22.202678777615272
37	21.53471634208298	28.804941882841966	27.659341082287735	22.001000692787315
38	22.220575783234548	29.373219921484107	26.00992995150489	22.396274343776458
39	23.36001847432838	26.599953814179045	26.547225001924406	23.49280270956816
40	23.65137402817335	26.374990377953967	27.751712724193673	22.22192286967901
41	20.048687552921255	26.625933338465092	27.920868293433916	25.404510815179737
42	20.86964052036025	27.422638749903776	27.30236317450543	24.405357555230545
43	22.43707181895158	25.604649372642598	27.63413132168424	24.324147486721575
44	21.388461242398584	26.919405742437068	27.77172657993996	23.920406435224386
45	21.28800708182588	27.090485720883688	26.907474405357558	24.714032791932876
46	22.417057963205295	28.67292741128474	25.68701408667539	23.22300053883458
47	21.05784774074359	27.570048495111998	28.14698637518282	23.22511738896159
48	21.211030713570935	26.05553844969594	29.106881687321994	23.62654914941113
49	21.424255253637135	26.006658455854055	29.205026556847045	23.364059733661765
50	20.551728119467324	25.593680240166268	28.99468863059041	24.859903009775998
51	20.207836194288355	25.72300053883458	27.620275575398352	26.448887691478717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	267.0
1	624.0
2	981.0
3	3233.5
4	5486.0
5	3763.5
6	2041.0
7	1902.5
8	1764.0
9	1655.5
10	1547.0
11	1570.5
12	1594.0
13	1512.0
14	1430.0
15	1335.5
16	1241.0
17	1289.0
18	1337.0
19	1477.5
20	1618.0
21	1864.5
22	2111.0
23	2412.0
24	2713.0
25	3124.0
26	4527.0
27	5519.0
28	6615.5
29	7712.0
30	8983.5
31	10255.0
32	11753.0
33	13251.0
34	14564.5
35	15878.0
36	16424.5
37	16971.0
38	18212.0
39	19453.0
40	23458.5
41	27464.0
42	29381.5
43	31299.0
44	33220.0
45	35141.0
46	39318.5
47	43496.0
48	42357.0
49	41218.0
50	39341.5
51	37465.0
52	33904.0
53	30343.0
54	28304.5
55	26266.0
56	24731.0
57	23196.0
58	22973.5
59	22751.0
60	22790.5
61	22830.0
62	21224.5
63	19619.0
64	16887.5
65	14156.0
66	11470.5
67	8785.0
68	7850.0
69	6915.0
70	5655.5
71	4396.0
72	3740.5
73	3085.0
74	2610.5
75	1587.0
76	1038.0
77	805.5
78	573.0
79	455.0
80	337.0
81	257.5
82	178.0
83	139.0
84	100.0
85	77.5
86	55.0
87	50.0
88	45.0
89	39.0
90	33.0
91	21.5
92	10.0
93	5.5
94	1.0
95	1.0
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	519640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.583932668683378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67383216988799	22.532355225277335
2	9.180927484369482	5.6157773603604175
3	3.5325425738644602	3.241171326849844
4	2.136806752305773	2.6140781535403104
5	1.5023611256560778	2.297405575555642
6	1.0979468989971934	2.014772041963192
7	0.790625919392287	1.6926314919366718
8	0.637457115024259	1.559675638806007
9	0.5247768017933953	1.4444764534922583
>10	6.5900077824151	47.61771584627701
>50	0.2831219522803746	5.269691770924543
>100	0.045826834848042904	2.4212512450588193
>500	6.277648609320946E-4	0.14438037715361446
>1k	0.003138824304660473	1.534617492804309
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2946	0.5669309521976753	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	1492	0.28712185359094755	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	1413	0.2719190208605958	No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	1072	0.20629666692325455	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	1070	0.20591178508197983	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	752	0.144715572319298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09525825571549534	0.0
2	0.0	0.0	0.0	0.5253637133400046	0.0
3	0.0	0.0	0.0	0.6837425910245555	0.0
4	0.0	0.0	0.0	0.9369948425833269	0.0
5	0.0	0.0	0.0	1.7502501731968285	0.0
6	0.0	0.0	0.0	2.1322454006619966	0.0
7	0.0	0.0	0.0	2.542144561619583	0.0
8	0.0	0.0	0.0	3.2401277807713034	0.0
9	0.0	0.0	0.0	3.4891463320760527	0.0
10	0.0	0.0	0.0	4.3557077977061045	0.0
11	0.0	0.0	0.0	5.157801554922639	0.0
12	0.0	0.0	0.0	6.077284273727965	0.0
13	0.0	0.0	0.0	6.362674159033177	0.0
14	0.0	0.0	0.0	6.4833346162728045	0.0
15	0.0	0.0	0.0	6.734854899545839	0.0
16	0.0	0.0	0.0	7.220768224155185	0.0
17	0.0	0.0	0.0	7.794627049495805	0.0
18	0.0	0.0	0.0	8.425063505503811	0.0
19	0.0	0.0	0.0	8.780309445000384	0.0
20	0.0	0.0	0.0	9.146332076052651	0.0
21	0.0	0.0	0.0	9.572203833423139	0.0
22	0.0	0.0	0.0	10.024247556000308	0.0
23	0.0	0.0	0.0	10.47167269648218	0.0
24	0.0	0.0	0.0	10.85944115156647	0.0
25	0.0	0.0	0.0	11.200831344777153	0.0
26	0.0	0.0	0.0	11.512970518050958	0.0
27	0.0	0.0	0.0	11.829343391578785	0.0
28	0.0	0.0	0.0	12.164575475329073	0.0
29	0.0	0.0	0.0	12.512893541682704	0.0
30	0.0	0.0	0.0	12.910668924640136	0.0
31	0.0	0.0	0.0	13.281887460549612	0.0
32	0.0	0.0	0.0	13.63251481795089	0.0
33	0.0	0.0	0.0	13.976791624971133	0.0
34	0.0	0.0	0.0	14.328958509737511	0.0
35	0.0	0.0	0.0	14.728080979139405	0.0
36	0.0	0.0	0.0	15.094296051112309	0.0
37	0.0	0.0	0.0	15.462243091370949	0.0
38	0.0	0.0	0.0	15.827880840581942	0.0
39	0.0	0.0	0.0	16.19294126703102	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGT	20	7.0297153E-4	45.0	37
GTCGACT	25	3.8878447E-5	45.0	12
ATATGCG	20	7.0297153E-4	45.0	29
CTAAGCG	20	7.0297153E-4	45.0	1
TCGATAG	20	7.0297153E-4	45.0	1
CCGTCAC	20	7.0297153E-4	45.0	11
ACGTGCG	20	7.0297153E-4	45.0	1
ACGCTAG	20	7.0297153E-4	45.0	1
ACCGTGC	20	7.0297153E-4	45.0	23
GCTACGC	20	7.0297153E-4	45.0	41
AATAGCG	50	2.1827873E-11	45.0	1
AAGGCGA	45	3.8380676E-10	45.0	6
CACAACG	85	0.0	42.35294	12
CATAGGA	50	1.0786607E-9	40.5	4
CGTTTTT	1400	0.0	40.017857	1
GTTACGG	45	1.9248546E-8	40.0	2
CTCGCGT	45	1.9248546E-8	40.0	12
GCGATAT	45	1.9248546E-8	40.0	9
TCGTTCA	40	3.4533514E-7	39.375	16
ACGCTTT	35	6.241682E-6	38.571426	34
>>END_MODULE