##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359920
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13559679928873	31.0	31.0	33.0	28.0	34.0
2	31.18220993554123	31.0	31.0	34.0	28.0	34.0
3	30.838561346965992	31.0	30.0	34.0	27.0	34.0
4	34.8294232051567	35.0	35.0	37.0	32.0	37.0
5	35.158390753500775	35.0	35.0	37.0	33.0	37.0
6	35.17805901311402	36.0	35.0	37.0	33.0	37.0
7	31.11724272060458	35.0	35.0	37.0	0.0	37.0
8	33.1669148699711	36.0	35.0	37.0	17.0	37.0
9	36.45763503000667	39.0	35.0	39.0	32.0	39.0
10	36.22727272727273	37.0	35.0	39.0	32.0	39.0
11	36.513997555012224	38.0	35.0	39.0	32.0	39.0
12	36.52014614358746	38.0	35.0	39.0	32.0	39.0
13	36.43529951100245	39.0	35.0	39.0	32.0	39.0
14	37.461144143142924	39.0	36.0	41.0	32.0	41.0
15	37.723621915981326	39.0	36.0	41.0	33.0	41.0
16	37.6276061346966	39.0	36.0	41.0	33.0	41.0
17	37.536913758613025	39.0	36.0	41.0	33.0	41.0
18	37.434963325183375	39.0	36.0	40.0	33.0	41.0
19	37.159974438764166	39.0	36.0	40.0	32.0	41.0
20	37.06131084685486	39.0	35.0	40.0	32.0	41.0
21	37.01830962436097	39.0	35.0	40.0	32.0	41.0
22	37.0885530117804	39.0	35.0	40.0	32.0	41.0
23	37.15018059568793	39.0	35.0	40.0	32.0	41.0
24	37.076800400088906	39.0	35.0	40.0	32.0	41.0
25	36.754389864414314	38.0	35.0	40.0	31.0	41.0
26	36.71990720160036	38.0	35.0	40.0	31.0	41.0
27	36.754567681707044	38.0	35.0	40.0	31.0	41.0
28	36.76028839742165	38.0	35.0	40.0	31.0	41.0
29	36.72928984218715	38.0	35.0	40.0	31.0	41.0
30	36.511574794398754	38.0	35.0	40.0	31.0	41.0
31	36.40537341631474	38.0	35.0	40.0	31.0	41.0
32	36.14936930428984	38.0	35.0	40.0	30.0	41.0
33	35.92775338964214	38.0	35.0	40.0	30.0	41.0
34	35.792601133585244	38.0	35.0	40.0	29.0	41.0
35	35.644954434318734	38.0	35.0	40.0	27.0	41.0
36	35.53512725050011	38.0	35.0	40.0	27.0	41.0
37	35.376386419204266	38.0	35.0	40.0	26.0	41.0
38	35.2281256945988	38.0	34.0	40.0	25.0	41.0
39	35.16646476994888	38.0	34.0	40.0	25.0	41.0
40	34.99408201822627	38.0	34.0	40.0	24.0	41.0
41	34.838441875972435	38.0	34.0	40.0	23.0	41.0
42	34.78000111135808	38.0	34.0	40.0	23.0	41.0
43	34.63822238275172	38.0	34.0	40.0	23.0	41.0
44	34.3942709490998	38.0	34.0	40.0	22.0	41.0
45	34.30817681707046	37.0	33.0	40.0	22.0	41.0
46	34.140586797066014	37.0	33.0	40.0	21.0	41.0
47	34.07047677261614	37.0	33.0	40.0	21.0	41.0
48	34.0028534118693	37.0	33.0	40.0	21.0	41.0
49	33.86500889086464	37.0	33.0	40.0	20.0	41.0
50	33.69229828850856	37.0	33.0	40.0	20.0	41.0
51	31.983110135585687	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	26.0
10	40.0
11	15.0
12	9.0
13	11.0
14	11.0
15	22.0
16	37.0
17	88.0
18	181.0
19	375.0
20	523.0
21	891.0
22	1418.0
23	2123.0
24	3125.0
25	4584.0
26	6267.0
27	7024.0
28	7268.0
29	7717.0
30	8751.0
31	10828.0
32	13543.0
33	19233.0
34	29691.0
35	33107.0
36	34567.0
37	50409.0
38	66890.0
39	51129.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.418870860191156	21.005223382973995	24.881640364525452	16.6942653923094
2	36.357523894198714	23.295454545454543	24.882196043565237	15.464825516781508
3	31.421704823294068	22.146032451655923	31.661758168481885	14.770504556568126
4	28.230995776839297	24.688264058679707	29.850244498777506	17.23049566570349
5	24.131751500333408	28.783896421426984	29.541286952656147	17.543065125583464
6	25.55401200266726	32.27411647032674	29.073683040675707	13.098188486330296
7	65.43981995999111	17.777561680373417	13.087908424094245	3.6947099355412316
8	75.3570237830629	5.751555901311402	14.074238719715492	4.817181595910202
9	69.62324961102468	8.571349188708602	16.371971549233162	5.433429651033563
10	38.70610135585686	24.520171149144254	23.409368748610802	13.364358746388087
11	31.290842409424318	24.10257835074461	28.219882196043567	16.386697043787507
12	28.026228050677926	21.35835741275839	33.05817959546566	17.55723494109802
13	22.52611691486997	25.0083351855968	33.56968215158924	18.895865747943986
14	17.525561235830185	29.239553234052014	33.89086463658591	19.344020893531898
15	18.616914869971104	24.644643254056454	39.45348966436986	17.28495221160258
16	20.65375639030896	23.128472993998667	37.512224938875306	18.70554567681707
17	19.162869526561458	23.25905756834852	33.55190042231607	24.02617248277395
18	20.816292509446544	25.107246054678818	34.159535452322736	19.9169259835519
19	24.28900866859302	25.03861969326517	31.0588464103134	19.613525227828408
20	25.159202044898866	24.979439875527895	31.121082462769508	18.740275616803732
21	22.38691931540342	25.873249611024672	32.204934429873305	19.5348966436986
22	20.307290509002	24.780784618804176	31.32973994220938	23.58218492998444
23	20.65709046454768	25.94771060235608	31.440320071126916	21.954878861969327
24	21.10413425205601	23.68220715714603	34.231773727494996	20.98188486330296
25	20.01583685263392	25.476216937097135	32.23799733274061	22.26994887752834
26	18.407979551011337	26.9351522560569	34.04756612580574	20.609302067126027
27	20.259779951100246	26.104412091575906	33.71610357857301	19.919704378750833
28	18.06818181818182	27.286619248721937	34.52072682818404	20.124472104912204
29	19.907201600355634	24.996110246721493	34.82329406534785	20.273394087575017
30	20.312569459879974	24.519337630584577	33.424649922204935	21.743442987330518
31	22.74255390086686	26.276672593909755	30.767948432985108	20.212825072238275
32	22.949544343187377	25.807401644809957	31.15803511891532	20.08501889308735
33	21.461991553678594	26.330851300288955	30.096132473883085	22.111024672149366
34	19.334852189375418	25.772949544343188	33.21543676372527	21.676761502556126
35	20.283951989330962	23.27406090242276	32.61808179595466	23.823905312291622
36	21.00883529673261	26.447543898644142	30.96382529450989	21.57979551011336
37	20.480662369415427	27.287452767281618	32.08268504112025	20.149199822182705
38	20.48260724605468	27.459157590575682	27.87063791953768	24.187597243831963
39	22.004334296510336	24.280395643476325	30.3156256945988	23.399644365414538
40	20.969937763947545	23.70304512113803	32.519448766392536	22.807568348521894
41	18.169315403422985	24.59685485663481	31.381140253389646	25.852689486552567
42	20.244220937986217	25.539286508112912	30.346188041787066	23.8703045121138
43	22.076572571682597	24.435152256056902	30.30367859524339	23.184596577017114
44	20.489275394532118	26.007723938653033	30.257001555901315	23.245999110913537
45	21.16525894643254	25.241720382307182	29.479050900200043	24.113969771060237
46	20.749055345632364	25.894365414536562	29.963880862413873	23.392698377417204
47	19.384307623916424	24.299288730829076	32.26161369193154	24.05478995332296
48	20.27089353189598	23.79195376750389	31.593965325627916	24.343187374972217
49	20.09390975772394	23.838075127806178	32.58057346076906	23.48744165370082
50	19.211213603022895	23.886697043787507	31.9684929984441	24.9335963547455
51	18.695821293620803	24.460713491887088	29.623249611024672	27.220215603467434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	291.0
1	522.5
2	754.0
3	5127.5
4	9501.0
5	6390.0
6	3279.0
7	2974.0
8	2669.0
9	2579.0
10	2489.0
11	2380.0
12	2271.0
13	2273.5
14	2276.0
15	2013.0
16	1750.0
17	1622.5
18	1495.0
19	1556.0
20	1617.0
21	1799.5
22	1982.0
23	1885.0
24	1788.0
25	2341.5
26	3377.5
27	3860.0
28	4708.0
29	5556.0
30	6617.0
31	7678.0
32	7640.0
33	7602.0
34	8364.5
35	9127.0
36	9793.5
37	10460.0
38	11310.0
39	12160.0
40	14482.5
41	16805.0
42	19361.0
43	21917.0
44	23756.5
45	25596.0
46	28729.0
47	31862.0
48	32500.0
49	33138.0
50	31604.5
51	30071.0
52	25293.5
53	20516.0
54	18149.5
55	15783.0
56	14941.5
57	14100.0
58	14178.5
59	14257.0
60	13574.0
61	12891.0
62	11950.0
63	11009.0
64	9132.0
65	7255.0
66	5698.5
67	4142.0
68	3652.0
69	3162.0
70	2770.5
71	2379.0
72	1929.5
73	1480.0
74	1238.0
75	744.5
76	493.0
77	418.5
78	344.0
79	219.0
80	94.0
81	73.0
82	52.0
83	41.0
84	30.0
85	27.0
86	24.0
87	13.5
88	3.0
89	6.0
90	9.0
91	7.0
92	5.0
93	5.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	359920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.02845465273824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84694821717494	27.32648334391073
2	9.553095437913724	6.883665315563103
3	3.691840036702559	3.9903387404250488
4	2.001385851748375	2.884273576093929
5	1.3323188931656782	2.400069541270302
6	0.8511998312562417	1.8400448712500368
7	0.6367758464236037	1.6059434794782275
8	0.5273717250099821	1.5200310623726785
9	0.3779999994345326	1.2256880254525926
>10	4.924705509102174	40.91778684690243
>50	0.21555682456182937	4.7620547156414155
>100	0.03772244429832013	2.4815716431267836
>500	0.0023095374060196	0.5863465355504661
>1k	0.0	0.0
>5k	7.698458020065334E-4	1.5757023029622508
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5681	1.578406312513892	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	824	0.22893976439208713	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	695	0.19309846632585018	TruSeq Adapter, Index 20 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	595	0.16531451433651922	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT	442	0.12280506779284285	TruSeq Adapter, Index 22 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	418	0.11613691931540342	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	391	0.10863525227828408	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	364	0.10113358524116471	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.778395198933096E-4	0.0	0.0	0.16198044009779952	0.0
2	2.778395198933096E-4	0.0	0.0	0.9577128250722383	0.0
3	2.778395198933096E-4	0.0	0.0	1.2825072238275173	0.0
4	2.778395198933096E-4	0.0	0.0	1.724827739497666	0.0
5	2.778395198933096E-4	0.0	0.0	2.8047899533229605	0.0
6	2.778395198933096E-4	0.0	0.0	3.381306957101578	0.0
7	2.778395198933096E-4	0.0	0.0	4.063402978439653	0.0
8	2.778395198933096E-4	0.0	0.0	5.320348966436986	0.0
9	2.778395198933096E-4	0.0	0.0	5.740164480995777	0.0
10	2.778395198933096E-4	0.0	0.0	6.767892865081129	0.0
11	2.778395198933096E-4	0.0	0.0	8.129028673038453	0.0
12	2.778395198933096E-4	0.0	0.0	9.274838853078462	0.0
13	2.778395198933096E-4	0.0	0.0	9.727717270504556	0.0
14	2.778395198933096E-4	0.0	0.0	9.922482773949767	0.0
15	2.778395198933096E-4	0.0	0.0	10.283396310291176	0.0
16	2.778395198933096E-4	0.0	0.0	11.007723938653035	0.0
17	2.778395198933096E-4	0.0	0.0	11.839575461213602	0.0
18	2.778395198933096E-4	0.0	0.0	12.867859524338742	0.0
19	2.778395198933096E-4	0.0	0.0	13.375750166703712	0.0
20	2.778395198933096E-4	0.0	0.0	13.929206490331184	0.0
21	2.778395198933096E-4	0.0	0.0	14.600744609913313	0.0
22	2.778395198933096E-4	0.0	0.0	15.219215381195822	0.0
23	2.778395198933096E-4	0.0	0.0	15.856579239831074	0.0
24	2.778395198933096E-4	0.0	0.0	16.38169593242943	0.0
25	2.778395198933096E-4	0.0	0.0	16.823182929539897	0.0
26	2.778395198933096E-4	0.0	0.0	17.250777950655703	0.0
27	5.556790397866192E-4	0.0	0.0	17.657257168259612	0.0
28	5.556790397866192E-4	0.0	0.0	18.08179595465659	0.0
29	5.556790397866192E-4	0.0	0.0	18.531618137363857	0.0
30	5.556790397866192E-4	0.0	0.0	19.049511002444987	0.0
31	5.556790397866192E-4	0.0	0.0	19.550455656812623	0.0
32	5.556790397866192E-4	0.0	0.0	20.03611913758613	0.0
33	5.556790397866192E-4	0.0	0.0	20.467881751500332	0.0
34	5.556790397866192E-4	0.0	0.0	20.947432762836186	0.0
35	5.556790397866192E-4	0.0	0.0	21.40531229162036	0.0
36	5.556790397866192E-4	0.0	0.0	21.886530340075574	0.0
37	5.556790397866192E-4	0.0	0.0	22.345243387419426	0.0
38	5.556790397866192E-4	0.0	0.0	22.79367637252723	0.0
39	5.556790397866192E-4	0.0	0.0	23.25905756834852	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	20	7.026822E-4	45.000004	14
AACCGTC	20	7.026822E-4	45.000004	10
AGTCCGT	20	7.026822E-4	45.000004	33
GTCGACC	20	7.026822E-4	45.000004	38
CCGGCTA	25	3.8854476E-5	45.000004	15
GATCCGC	25	3.8854476E-5	45.000004	17
CGACCTA	20	7.026822E-4	45.000004	12
CCGTCGA	20	7.026822E-4	45.000004	36
ACGATAT	20	7.026822E-4	45.000004	13
AGTTCAC	25	3.8854476E-5	45.000004	41
GTTCACC	20	7.026822E-4	45.000004	42
ATGCGTG	20	7.026822E-4	45.000004	21
CATCGAT	20	7.026822E-4	45.000004	44
CGTCGAC	20	7.026822E-4	45.000004	37
ACCGTTT	25	3.8854476E-5	45.000004	18
ACGTATA	20	7.026822E-4	45.000004	40
ACCGTGC	20	7.026822E-4	45.000004	12
CGGTCTA	25	3.8854476E-5	45.000004	31
AATAGGC	20	7.026822E-4	45.000004	4
ATACGAG	50	2.1827873E-11	45.000004	1
>>END_MODULE