FastQCFastQC Report
Sat 18 Jun 2016
SRR3551426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206728
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGC10830.5238767849541426No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.48034131806044655No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCG9650.4667969505824078No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC8710.42132657404899193No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6840.3308695483920901No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC5810.2810456251693046No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTT3370.16301613714639526No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCT2470.11948067025269919No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA2420.11706203320304943No Hit
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC2240.1083549398243102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGG302.1568376E-645.0000042
GCGTACG351.2054625E-745.0000041
CGAGTAA207.0198515E-445.024
CGGCACT453.8198777E-1045.017
GCACGAT207.0198515E-445.041
ATTGTCG253.8796737E-545.011
GTTCGGG502.1827873E-1145.03
CGTGTAC253.8796737E-545.018
TATAGCG253.8796737E-545.01
CGCACGA207.0198515E-445.040
GTGAAAC253.8796737E-545.037
TGGCTAC207.0198515E-445.041
GCCCGTG253.8796737E-545.023
CCGCACG207.0198515E-445.039
TACGGGA800.042.18754
GGGACCG700.041.7857177
GACCGAT556.002665E-1140.9090929
CGTTTTT5050.040.5445561
TTTCGGC501.0732037E-940.514
CATATCA451.915214E-840.029