##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	206728
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201820749970977	31.0	31.0	33.0	28.0	34.0
2	31.227356719941177	31.0	31.0	34.0	28.0	34.0
3	31.04648136682017	31.0	31.0	34.0	28.0	34.0
4	35.01092256491622	35.0	35.0	37.0	32.0	37.0
5	35.23451104833404	35.0	35.0	37.0	33.0	37.0
6	35.22447854185209	36.0	35.0	37.0	33.0	37.0
7	31.14966526063233	35.0	35.0	37.0	0.0	37.0
8	33.1388055802794	36.0	35.0	37.0	17.0	37.0
9	36.41212124143802	39.0	35.0	39.0	32.0	39.0
10	36.212883595836075	37.0	35.0	39.0	32.0	39.0
11	36.463164157733836	38.0	35.0	39.0	32.0	39.0
12	36.48349522077319	38.0	35.0	39.0	32.0	39.0
13	36.308202081962776	38.0	35.0	39.0	32.0	39.0
14	37.409615533454584	39.0	36.0	41.0	32.0	41.0
15	37.62621415579893	39.0	36.0	41.0	33.0	41.0
16	37.59518787972602	39.0	36.0	41.0	33.0	41.0
17	37.47506385201811	39.0	36.0	41.0	32.0	41.0
18	37.42025269919895	39.0	36.0	40.0	32.0	41.0
19	37.25079815022639	39.0	36.0	40.0	32.0	41.0
20	37.20173367903719	39.0	35.0	40.0	32.0	41.0
21	37.21496362369877	39.0	35.0	40.0	32.0	41.0
22	37.26620003095855	39.0	35.0	40.0	32.0	41.0
23	37.32838318950505	39.0	35.0	40.0	32.0	41.0
24	37.27721934135676	39.0	35.0	41.0	32.0	41.0
25	36.968272319182695	39.0	35.0	40.0	32.0	41.0
26	36.93322143105917	39.0	35.0	40.0	32.0	41.0
27	36.963846213381835	39.0	35.0	40.0	32.0	41.0
28	36.93016427382841	39.0	35.0	40.0	31.0	41.0
29	36.90903989783677	39.0	35.0	40.0	31.0	41.0
30	36.72398997716807	39.0	35.0	40.0	31.0	41.0
31	36.689722727448626	39.0	35.0	40.0	31.0	41.0
32	36.52019561936458	39.0	35.0	40.0	31.0	41.0
33	36.366138113850084	39.0	35.0	40.0	30.0	41.0
34	36.30835687473395	39.0	35.0	40.0	30.0	41.0
35	36.23842440308037	39.0	35.0	40.0	30.0	41.0
36	36.15219031771216	39.0	35.0	40.0	30.0	41.0
37	36.06898436593011	39.0	35.0	40.0	30.0	41.0
38	35.92549146704849	38.0	35.0	40.0	29.0	41.0
39	35.90227738864595	38.0	35.0	40.0	29.0	41.0
40	35.800646259819665	38.0	35.0	40.0	29.0	41.0
41	35.65629232614837	38.0	35.0	40.0	28.0	41.0
42	35.67978696644867	38.0	35.0	40.0	28.0	41.0
43	35.63525985836461	38.0	35.0	40.0	28.0	41.0
44	35.44964397662629	38.0	34.0	40.0	27.0	41.0
45	35.34378990751132	38.0	34.0	40.0	27.0	41.0
46	35.23222785495917	38.0	34.0	40.0	27.0	41.0
47	35.1989135482373	38.0	34.0	40.0	27.0	41.0
48	35.11594946016021	38.0	34.0	40.0	26.0	41.0
49	34.99512402770791	37.0	34.0	40.0	26.0	41.0
50	34.849222166324836	37.0	34.0	40.0	26.0	41.0
51	33.25580472891916	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	15.0
10	19.0
11	9.0
12	6.0
13	11.0
14	12.0
15	13.0
16	25.0
17	59.0
18	101.0
19	183.0
20	318.0
21	440.0
22	689.0
23	1022.0
24	1380.0
25	1949.0
26	2482.0
27	2930.0
28	3169.0
29	3754.0
30	4420.0
31	5728.0
32	7481.0
33	10603.0
34	16904.0
35	18512.0
36	19678.0
37	30392.0
38	40628.0
39	33776.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.697863859757746	19.178340621492975	22.32982469718664	13.793970821562631
2	32.24285050888124	26.30509655199102	23.91548314693704	17.536569792190704
3	32.95828334816764	24.006907627413803	27.9081691884989	15.126639835919661
4	29.01058395572927	25.180914051313803	27.362040942687976	18.446461050268955
5	26.671278201307995	29.689737239270926	24.86262141557989	18.77636314384118
6	25.92246817073643	36.55769900545644	24.0925273789714	13.427305444835728
7	69.12271196935103	17.69571611005766	9.867071707751247	3.31450021284006
8	79.34290468635115	7.570817692813746	9.29965945590341	3.7866181649316975
9	73.20537130915986	8.473453039743044	11.092836964513756	7.228338686583337
10	41.29339034867072	27.66001702720483	17.466429317750862	13.58016330637359
11	33.49957431987926	25.69076274137998	23.414825277659535	17.394837661081226
12	31.222185673929026	22.057002437986146	28.058124685577184	18.662687202507644
13	25.448415309005068	25.97664564064858	28.902229015904958	19.67271003444139
14	19.951820749970977	29.539298014782712	28.32707712549824	22.181804109748075
15	19.6146627452498	27.080027862698813	34.026353469292985	19.278955922758406
16	22.065709531364885	25.22057969892806	32.3874850044503	20.326225765256762
17	21.405905344220425	25.059014744011453	29.95240122286289	23.58267868890523
18	23.495124027707906	25.13302503773074	29.32403931736388	22.04781161719748
19	26.102898494640296	27.250783638404087	26.076777214504084	20.569540652451533
20	26.953775008707094	25.936012538214463	28.368677682752214	18.741534770326226
21	26.40619558066638	27.399771680662514	26.911206996633258	19.282825742037847
22	22.46720328160675	25.152374134127935	27.76256723810998	24.617855346155334
23	22.205023025424715	27.840447351108704	27.928969467125885	22.0255601563407
24	22.8991718586742	25.170272048295345	29.584768391316125	22.34578770171433
25	21.410258890909795	28.109883518439688	28.07166905305522	22.408188537595294
26	20.757226887504356	29.785515266437056	27.099376959096013	22.357880886962576
27	22.034267249719438	29.444003715026508	27.673561394682867	20.848167640571187
28	20.424906156882475	29.024128323207304	30.29294531945358	20.258020200456638
29	21.01215123253744	28.052319956658025	29.263573391122634	21.6719554196819
30	21.966061684919314	28.68551913625634	28.608606478077476	20.739812700746878
31	25.431001122247594	26.93249100267018	24.89938469873457	22.737123176347666
32	25.360860647807748	30.023992879532525	25.291687628187766	19.323458844471965
33	24.575771061491427	28.411729422235982	24.385182461979028	22.627317054293563
34	22.398513989396697	28.560233737084477	26.539704345807053	22.501547927711776
35	22.974633334623274	27.98653302890755	25.709144382957316	23.329689253511862
36	23.277446693239426	30.73071862544019	24.80215548933865	21.18967919198173
37	23.414825277659535	30.165725010642003	27.683719670291396	18.735730041407066
38	22.89723694903448	29.771487171549087	25.212356332959253	22.11891954645718
39	23.78923029294532	27.011338570488757	26.797531055299718	22.401900081266206
40	24.78474130258117	27.076158043419372	26.79994969234937	21.33915096165009
41	20.64113230912116	27.727255137185093	27.052939127742736	24.57867342595101
42	22.448821640029408	27.907685461088967	27.125498239232225	22.517994659649396
43	23.548817770210132	27.00166402229016	27.096958322046362	22.35255988545335
44	22.43527727255137	27.612611741031696	26.78301923300182	23.169091753415117
45	21.881409388181574	27.396385588793002	25.587728803064895	25.13447621996053
46	22.593456135598466	29.66603459618436	25.447447854185214	22.293061414031964
47	21.90898185054758	27.26529546070198	28.949150574668163	21.876572114082272
48	22.74970008900584	26.353469292984016	28.280155566735033	22.616675051275106
49	21.4378313532758	26.584207267520604	29.059440424132195	22.918520955071397
50	20.980225223482062	27.42395805115901	27.976374753299023	23.619441972059903
51	20.46989280600596	26.54502534731628	26.78930768933091	26.19577415734685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	268.5
2	428.0
3	1246.5
4	2065.0
5	1462.5
6	860.0
7	820.5
8	781.0
9	745.0
10	709.0
11	726.0
12	743.0
13	710.0
14	677.0
15	657.5
16	638.0
17	663.5
18	689.0
19	630.5
20	572.0
21	665.0
22	758.0
23	820.0
24	882.0
25	995.5
26	1435.5
27	1762.0
28	2290.5
29	2819.0
30	3280.0
31	3741.0
32	4260.5
33	4780.0
34	5161.5
35	5543.0
36	6053.5
37	6564.0
38	6893.5
39	7223.0
40	8429.0
41	9635.0
42	11010.5
43	12386.0
44	13535.5
45	14685.0
46	17640.0
47	20595.0
48	19000.5
49	17406.0
50	16537.0
51	15668.0
52	14058.5
53	12449.0
54	11784.0
55	11119.0
56	10301.5
57	9484.0
58	9170.5
59	8857.0
60	8922.5
61	8988.0
62	8180.5
63	7373.0
64	6294.0
65	5215.0
66	4304.0
67	3393.0
68	2733.0
69	2073.0
70	1821.5
71	1570.0
72	1304.5
73	1039.0
74	868.0
75	487.5
76	278.0
77	225.5
78	173.0
79	137.0
80	101.0
81	68.5
82	36.0
83	28.0
84	20.0
85	15.5
86	11.0
87	14.5
88	18.0
89	11.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	206728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.666673116365466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75711011351768	25.950040633102432
2	8.68539188694343	6.19558066638288
3	3.745948218572417	4.008165318679618
4	2.203897847639456	3.1442281645447157
5	1.544762860591594	2.754827599551101
6	1.159589329065683	2.481521612940676
7	0.9805650115958933	2.448144421655509
8	0.9371651770577625	2.674045122092798
9	0.7947594699795206	2.551178359970589
>10	7.101297926301656	42.42337757826709
>50	0.05289354834334694	1.2852637281838937
>100	0.02848114141564835	1.5793699934213072
>500	0.006781224146582941	1.980380016253241
>1k	0.0013562448293165882	0.5238767849541426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGC	1083	0.5238767849541426	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	993	0.48034131806044655	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCG	965	0.4667969505824078	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	871	0.42132657404899193	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	684	0.3308695483920901	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	581	0.2810456251693046	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTT	337	0.16301613714639526	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCT	247	0.11948067025269919	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	242	0.11706203320304943	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	224	0.1083549398243102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11512712356332959	0.0
2	0.0	0.0	0.0	0.6762509190820789	0.0
3	0.0	0.0	0.0	0.8349135095391045	0.0
4	0.0	0.0	0.0	1.116926589528269	0.0
5	0.0	0.0	0.0	2.0921210479470607	0.0
6	0.0	0.0	0.0	2.601486010603305	0.0
7	0.0	0.0	0.0	3.049901319608374	0.0
8	0.0	0.0	0.0	3.624569482605162	0.0
9	0.0	0.0	0.0	3.796292713130297	0.0
10	0.0	0.0	0.0	4.766166170039859	0.0
11	0.0	0.0	0.0	5.510138926512131	0.0
12	0.0	0.0	0.0	6.680759258542626	0.0
13	0.0	0.0	0.0	7.013563716574436	0.0
14	0.0	0.0	0.0	7.1702913973917415	0.0
15	0.0	0.0	0.0	7.452788204790837	0.0
16	0.0	0.0	0.0	7.894915057466816	0.0
17	0.0	0.0	0.0	8.394121744514532	0.0
18	0.0	0.0	0.0	8.96008281413258	0.0
19	0.0	0.0	0.0	9.259026353469293	0.0
20	0.0	0.0	0.0	9.593281993730892	0.0
21	0.0	0.0	0.0	9.977845284625207	0.0
22	0.0	0.0	0.0	10.383692581556442	0.0
23	0.0	0.0	0.0	10.775511783599706	0.0
24	0.0	0.0	0.0	11.098641693432917	0.0
25	0.0	0.0	0.0	11.414031964707249	0.0
26	0.0	0.0	0.0	11.702817228435432	0.0
27	0.0	0.0	0.0	11.993053674393407	0.0
28	0.0	0.0	0.0	12.27748539143222	0.0
29	0.0	0.0	0.0	12.582717387098022	0.0
30	0.0	0.0	0.0	12.918907936999343	0.0
31	0.0	0.0	0.0	13.249293757981503	0.0
32	0.0	0.0	0.0	13.573391122634574	0.0
33	0.0	0.0	0.0	13.864595023412406	0.0
34	0.0	0.0	0.0	14.182887659146317	0.0
35	0.0	0.0	0.0	14.499729112650439	0.0
36	0.0	0.0	0.0	14.810765837235401	0.0
37	0.0	0.0	0.0	15.136314384118261	0.0
38	0.0	0.0	0.0	15.453639565032313	0.0
39	0.0	0.0	0.0	15.81256530320034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTAGG	30	2.1568376E-6	45.000004	2
GCGTACG	35	1.2054625E-7	45.000004	1
CGAGTAA	20	7.0198515E-4	45.0	24
CGGCACT	45	3.8198777E-10	45.0	17
GCACGAT	20	7.0198515E-4	45.0	41
ATTGTCG	25	3.8796737E-5	45.0	11
GTTCGGG	50	2.1827873E-11	45.0	3
CGTGTAC	25	3.8796737E-5	45.0	18
TATAGCG	25	3.8796737E-5	45.0	1
CGCACGA	20	7.0198515E-4	45.0	40
GTGAAAC	25	3.8796737E-5	45.0	37
TGGCTAC	20	7.0198515E-4	45.0	41
GCCCGTG	25	3.8796737E-5	45.0	23
CCGCACG	20	7.0198515E-4	45.0	39
TACGGGA	80	0.0	42.1875	4
GGGACCG	70	0.0	41.785717	7
GACCGAT	55	6.002665E-11	40.909092	9
CGTTTTT	505	0.0	40.544556	1
TTTCGGC	50	1.0732037E-9	40.5	14
CATATCA	45	1.915214E-8	40.0	29
>>END_MODULE