##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551425_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387815
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20934981885693	31.0	31.0	33.0	30.0	34.0
2	31.23569485450537	31.0	31.0	34.0	28.0	34.0
3	31.02598919587948	31.0	31.0	34.0	28.0	34.0
4	34.97690909325323	35.0	35.0	37.0	32.0	37.0
5	35.20430101981615	35.0	35.0	37.0	33.0	37.0
6	35.16933073759395	36.0	35.0	37.0	32.0	37.0
7	31.000500238515787	35.0	35.0	37.0	0.0	37.0
8	32.978139061150294	35.0	35.0	37.0	17.0	37.0
9	36.1506465711744	39.0	35.0	39.0	32.0	39.0
10	36.129499890411665	37.0	35.0	39.0	32.0	39.0
11	36.35117517372974	37.0	35.0	39.0	32.0	39.0
12	36.40532470378918	38.0	35.0	39.0	32.0	39.0
13	36.247269961192835	38.0	35.0	39.0	32.0	39.0
14	37.40693371839666	39.0	36.0	40.0	32.0	41.0
15	37.51007568041463	39.0	36.0	41.0	32.0	41.0
16	37.571349741500455	39.0	36.0	41.0	33.0	41.0
17	37.351824968090455	39.0	36.0	41.0	32.0	41.0
18	37.27372587445045	39.0	36.0	40.0	32.0	41.0
19	37.06442762657453	39.0	35.0	40.0	32.0	41.0
20	36.97463997008883	39.0	35.0	40.0	31.0	41.0
21	37.116091435349325	39.0	35.0	40.0	32.0	41.0
22	37.16511738844552	39.0	35.0	40.0	32.0	41.0
23	37.242589894666274	39.0	35.0	40.0	32.0	41.0
24	37.139576860100824	39.0	35.0	40.0	32.0	41.0
25	36.91203537769297	39.0	35.0	40.0	31.0	41.0
26	36.773458994623724	39.0	35.0	40.0	31.0	41.0
27	36.838879362582674	39.0	35.0	40.0	31.0	41.0
28	36.80047703157433	39.0	35.0	40.0	31.0	41.0
29	36.73105217694003	39.0	35.0	40.0	31.0	41.0
30	36.54838518365715	39.0	35.0	40.0	31.0	41.0
31	36.55371246599538	39.0	35.0	40.0	31.0	41.0
32	36.3782009463275	38.0	35.0	40.0	30.0	41.0
33	36.07989634232817	38.0	35.0	40.0	30.0	41.0
34	36.025501850108945	38.0	35.0	40.0	30.0	41.0
35	36.01310418627438	38.0	35.0	40.0	29.0	41.0
36	35.93118368294161	38.0	35.0	40.0	29.0	41.0
37	35.950561479055736	38.0	35.0	40.0	29.0	41.0
38	35.66950736820391	38.0	35.0	40.0	28.0	41.0
39	35.69771153771772	38.0	35.0	40.0	28.0	41.0
40	35.48391630029782	38.0	35.0	40.0	27.0	41.0
41	35.40243930740173	38.0	34.0	40.0	27.0	41.0
42	35.510055052022224	38.0	35.0	40.0	27.0	41.0
43	35.464082101001765	38.0	35.0	40.0	27.0	41.0
44	35.319869009708235	38.0	34.0	40.0	27.0	41.0
45	35.2447584544177	38.0	34.0	40.0	26.0	41.0
46	35.123339736730145	38.0	34.0	40.0	26.0	41.0
47	35.01615460980106	38.0	34.0	40.0	26.0	41.0
48	34.98037724172608	37.0	34.0	40.0	26.0	41.0
49	34.873032244755876	37.0	34.0	40.0	26.0	41.0
50	34.723824503951626	37.0	34.0	40.0	25.0	41.0
51	33.0298647551023	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	19.0
9	44.0
10	45.0
11	24.0
12	17.0
13	25.0
14	21.0
15	29.0
16	57.0
17	114.0
18	225.0
19	409.0
20	703.0
21	1043.0
22	1524.0
23	2052.0
24	2823.0
25	3955.0
26	4997.0
27	5739.0
28	6199.0
29	7185.0
30	8672.0
31	10979.0
32	14826.0
33	20858.0
34	32046.0
35	34895.0
36	38093.0
37	56166.0
38	74245.0
39	59781.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.33136933847324	18.054484741435996	20.933692611167697	14.68045330892307
2	31.13469050965022	28.94240810695822	23.235563348503796	16.68733803488777
3	31.124376313448423	27.941157510668745	25.902298776478478	15.032167399404356
4	27.885202996273996	22.926653172259968	31.225455436226035	17.962688395239997
5	28.635818624859795	27.2034346273352	24.159457473279787	20.001289274525224
6	24.454959194461274	37.25513453579671	25.06323891546227	13.226667354279748
7	64.65273390663074	17.548315562832794	14.033237497260293	3.7657130332761755
8	73.76790479996906	10.01302167270477	11.357219292703997	4.861854234622178
9	68.53009811379137	7.689748978249939	12.21458685197839	11.5655660559803
10	41.440635354486034	23.74766318992303	20.224075912484047	14.587625543106894
11	33.14750589843095	24.87139486610884	24.59600582752085	17.385093407939355
12	30.533630725990484	20.83777058649098	29.13193146216624	19.496667225352294
13	24.297151992573777	24.07900674290577	32.38631821873831	19.237523045782137
14	20.06652656550159	29.064115622139425	28.436496783260058	22.432861029098927
15	18.601910704846382	25.431197864961387	36.83715173471887	19.129739695473358
16	21.858618155563864	23.981537588798783	31.465776207727913	22.69406804790944
17	20.951484599615796	24.656343875301367	31.00137952374199	23.390792001340845
18	22.284852313603135	25.96624679292962	30.11203795624202	21.636862937225224
19	24.396426131016078	27.26841406340652	26.512641336719827	21.822518468857574
20	26.73439655505847	24.49337957531297	30.044479971120253	18.727743898508308
21	25.013730773693645	28.265796836120316	27.551280894240808	19.16919149594523
22	22.66209403968387	23.576447532973198	28.654899887833118	25.106558539509816
23	22.94960225880897	28.122171654010288	28.955300852210463	19.972925234970283
24	24.36883565617627	23.738896123151505	28.19385531761278	23.69841290305945
25	22.045305106816397	29.31397702512796	26.86358186248598	21.777136005569666
26	20.95277387414102	27.206013176385646	28.09458117917048	23.74663177030285
27	23.893866921083507	28.064154300375176	26.234158039271303	21.807820739270014
28	19.18981988834883	28.067506414140762	31.272642883849255	21.470030813661154
29	23.705632840400707	26.598764875004832	28.744633394788753	20.950968889805708
30	23.787372845299952	26.170725732630252	29.86191869834844	20.179982723721363
31	25.169217281435735	27.249590655338242	25.016567177649137	22.564624885576887
32	27.265835514356073	28.242332039761227	25.17695292858708	19.31487951729562
33	23.636012016038574	25.76924564547529	27.145159418795046	23.44958291969109
34	24.45470133955623	27.966943001173238	27.137423771643697	20.44093188762683
35	23.952399984528704	25.108363523845135	25.98429663628276	24.954939855343397
36	22.890295630648634	30.72470121062878	26.37520467233088	20.00979848639171
37	26.442246947642563	27.028609001714738	26.876990317548316	19.652153733094387
38	21.799053672498488	28.7982672150381	26.424197104289416	22.978482008174
39	27.48707502288462	24.22443690935111	26.75012570426621	21.53836236349806
40	23.23839975245929	23.575673968258062	30.54858631048309	22.637339968799555
41	21.488854221729433	26.39454379020925	29.381019300439643	22.735582687621676
42	21.811946417750733	25.437902092492553	29.27684591880149	23.473305570955226
43	24.03594497376326	24.509624434330803	27.81790286605727	23.636527725848666
44	23.144024857212848	25.785748359398163	28.34805255082965	22.72217423255934
45	23.06357412683883	23.93125588231502	27.07760143367327	25.92756855717288
46	24.39410543687067	27.719144437425058	26.17227286206052	21.714477263643747
47	21.28850096050952	26.162732230573855	31.77984348207264	20.768923326843986
48	23.541894975697176	25.15632453618349	27.577839949460436	23.723940538658898
49	21.229710042159276	24.263888709822982	31.346905096502198	23.159496151515544
50	21.311707901963565	24.622049172930392	29.682451684437165	24.383791240668877
51	20.95947810167219	24.74478810773178	26.83547567783608	27.46025811275995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	163.0
1	568.5
2	974.0
3	2915.5
4	4857.0
5	3369.5
6	1882.0
7	1758.0
8	1634.0
9	1555.5
10	1477.0
11	1428.0
12	1379.0
13	1298.0
14	1217.0
15	1178.0
16	1139.0
17	1106.5
18	1074.0
19	1191.5
20	1309.0
21	1245.5
22	1182.0
23	1465.5
24	1749.0
25	1914.0
26	2358.0
27	2637.0
28	3493.0
29	4349.0
30	5181.5
31	6014.0
32	7033.5
33	8053.0
34	9149.5
35	10246.0
36	10962.0
37	11678.0
38	12542.5
39	13407.0
40	14956.0
41	16505.0
42	18428.0
43	20351.0
44	22545.0
45	24739.0
46	36445.0
47	48151.0
48	39007.5
49	29864.0
50	28985.0
51	28106.0
52	25482.0
53	22858.0
54	21577.0
55	20296.0
56	20110.0
57	19924.0
58	18972.0
59	18020.0
60	17931.5
61	17843.0
62	16366.5
63	14890.0
64	12281.0
65	9672.0
66	7872.0
67	6072.0
68	5084.5
69	4097.0
70	3470.0
71	2843.0
72	2517.0
73	2191.0
74	1727.0
75	1057.0
76	851.0
77	609.5
78	368.0
79	295.5
80	223.0
81	154.5
82	86.0
83	68.5
84	51.0
85	37.0
86	23.0
87	17.5
88	12.0
89	9.0
90	6.0
91	6.5
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	387815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.874528707860694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43412419389608	18.92883892661886
2	9.432012261379098	5.069617685826533
3	3.941870800653377	3.1780775998451105
4	2.3821559640892325	2.560772753740706
5	1.7134465714709612	2.3024034537190916
6	1.284454172685071	2.071146032265384
7	0.9812744053868276	1.8459901024500065
8	0.7549010567272963	1.6230088096489685
9	0.6681419728387441	1.6160400566983357
>10	8.101469993659292	47.38534534011286
>50	0.2423260962638645	3.9977557733027544
>100	0.05085856341340366	2.4645278602461707
>500	0.0069805871351730515	1.2062679315972178
>1k	0.0039889069343846005	2.6537358495169183
>5k	0.0019944534671923002	3.096471824411076
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	6170	1.5909647641272255	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	5384	1.3882908087619097	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	4710	1.214496602761626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2514	0.648247231282957	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	1522	0.3924551654783853	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	1156	0.29808027023194045	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	823	0.21221458685197842	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC	753	0.19416474349883323	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTA	706	0.18204556296172145	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	603	0.15548650774209352	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	573	0.1477508605907456	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	528	0.13614738986372368	No Hit
GAATCTATCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	515	0.13279527609813957	No Hit
CCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	392	0.10107912277761305	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	391	0.1008212678725681	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08277142451942292	0.0
2	0.0	0.0	0.0	0.7248301380813017	0.0
3	0.0	0.0	0.0	0.8612353828500703	0.0
4	0.0	0.0	0.0	1.259363356239444	0.0
5	0.0	0.0	0.0	3.032889393138481	0.0
6	0.0	0.0	0.0	3.4083261348839007	0.0
7	0.0	0.0	0.0	3.9753490710777046	0.0
8	0.0	0.0	0.0	4.642419710428942	0.0
9	0.0	0.0	0.0	4.819566030194809	0.0
10	0.0	0.0	0.0	7.025514742854196	0.0
11	0.0	0.0	0.0	7.616002475407089	0.0
12	0.0	0.0	0.0	9.715457112282918	0.0
13	0.0	0.0	0.0	10.00734886479378	0.0
14	0.0	0.0	0.0	10.162319662725784	0.0
15	0.0	0.0	0.0	10.567409718551371	0.0
16	0.0	0.0	0.0	10.950066397638048	0.0
17	0.0	0.0	0.0	11.363407810425073	0.0
18	0.0	0.0	0.0	11.814653894253704	0.0
19	0.0	0.0	0.0	12.139293219705271	0.0
20	0.0	0.0	0.0	12.416229387723527	0.0
21	0.0	0.0	0.0	12.735711615074198	0.0
22	0.0	0.0	0.0	13.071180846537652	0.0
23	0.0	0.0	0.0	13.394015187653908	0.0
24	0.0	0.0	0.0	13.654190786844243	0.0
25	0.0	0.0	0.0	13.880587393473693	0.0
26	0.0	0.0	0.0	14.11059396877377	0.0
27	0.0	0.0	0.0	14.365612469863208	0.0
28	0.0	0.0	0.0	14.619083841522375	0.0
29	0.0	0.0	0.0	14.863272436599924	0.0
30	0.0	0.0	0.0	15.171151193223572	0.0
31	0.0	0.0	0.0	15.438031019945077	0.0
32	0.0	0.0	0.0	15.686345293503345	0.0
33	0.0	0.0	0.0	15.944973763263413	0.0
34	0.0	0.0	0.0	16.21572141356059	0.0
35	0.0	0.0	0.0	16.50451890721091	0.0
36	0.0	0.0	0.0	16.787385738045202	0.0
37	0.0	0.0	0.0	17.089333831852816	0.0
38	0.0	0.0	0.0	17.363433595915577	0.0
39	0.0	0.0	0.0	17.663318850482835	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTAGG	20	7.027498E-4	45.000004	28
CCAATCC	20	7.027498E-4	45.000004	32
TCTAGAC	20	7.027498E-4	45.000004	28
GCGATAA	20	7.027498E-4	45.000004	9
TCATCGT	20	7.027498E-4	45.000004	17
TTACCGG	40	6.7957444E-9	45.000004	2
CACTTCG	20	7.027498E-4	45.000004	31
ACCGTTA	25	3.8860097E-5	45.0	43
CGATCAA	25	3.8860097E-5	45.0	10
GACGGGA	85	0.0	42.35294	4
ACACGGG	70	0.0	41.785713	3
AACGGCT	70	0.0	41.785713	7
GCTACGA	70	0.0	41.785713	10
TACGGCT	655	0.0	41.564884	7
CGAATAT	65	0.0	41.538464	14
CGGGATA	55	6.002665E-11	40.909092	6
TTAGCGG	50	1.0768417E-9	40.5	2
CGTTTTT	1240	0.0	40.463707	1
ACGGCTG	750	0.0	40.199997	8
CACGGGT	45	1.9226718E-8	40.0	4
>>END_MODULE