FastQCFastQC Report
Sat 18 Jun 2016
SRR3551424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312792
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG39401.2596230082610809No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC33171.0604491163456866No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC28950.9255351799278755TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.8101230210491316No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC13600.4347937287398655TruSeq Adapter, Index 23 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT6580.21036343640502314No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT6480.2071664236937006No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC5670.18127062073198802TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC5160.16496585590424306No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT4820.15409601268574644Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA4390.14034885802705951No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG4210.13459423514667895No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA3600.11509245760761146No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3600.11509245760761146No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT3540.11317424998081793Illumina PCR Primer Index 5 (95% over 24bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC3480.11125604235402439No Hit
GAATCTATCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC3440.10997723726949539No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGT207.025405E-445.026
CTCACGA351.2081364E-745.024
CGTATTC207.025405E-445.038
CGACGTT207.025405E-445.028
GAGTCGT253.884273E-545.030
TTTCGAC253.884273E-545.025
CGCATCG253.884273E-545.021
AGGGTGC207.025405E-445.07
CGACAGT207.025405E-445.044
CGACAAT207.025405E-445.020
TCCTATG207.025405E-445.031
ACGGGTA253.884273E-545.05
CATTATG207.025405E-445.042
CGTTATG253.884273E-545.026
GCCGATT253.884273E-545.09
CTACGAA207.025405E-445.011
AACGCTC207.025405E-445.023
GTCGTAG253.884273E-545.01
CGAATAT253.884273E-545.014
TCCCGGT207.025405E-445.017
Produced by FastQC (version 0.11.5)