##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	312792
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15350136832144	31.0	31.0	33.0	28.0	34.0
2	31.177347886135195	31.0	31.0	34.0	28.0	34.0
3	30.96142484462518	31.0	31.0	34.0	28.0	34.0
4	34.917923092662214	35.0	35.0	37.0	32.0	37.0
5	35.163086012429986	35.0	35.0	37.0	33.0	37.0
6	35.138110949129135	36.0	35.0	37.0	32.0	37.0
7	30.88829637586639	35.0	35.0	37.0	0.0	37.0
8	32.95037916570756	35.0	35.0	37.0	17.0	37.0
9	36.17865546433413	39.0	35.0	39.0	32.0	39.0
10	36.09942389830942	37.0	35.0	39.0	32.0	39.0
11	36.356633161973456	37.0	35.0	39.0	32.0	39.0
12	36.40492723599069	38.0	35.0	39.0	32.0	39.0
13	36.23291836108341	38.0	35.0	39.0	32.0	39.0
14	37.33669339369293	39.0	36.0	40.0	32.0	41.0
15	37.47457735491956	39.0	36.0	41.0	32.0	41.0
16	37.519041407708634	39.0	36.0	41.0	33.0	41.0
17	37.34990025320341	39.0	36.0	40.0	32.0	41.0
18	37.233858282820535	39.0	36.0	40.0	32.0	41.0
19	37.03207882554541	39.0	35.0	40.0	32.0	41.0
20	36.95060935062278	39.0	35.0	40.0	32.0	41.0
21	37.03855917031126	39.0	35.0	40.0	32.0	41.0
22	37.07176654134377	39.0	35.0	40.0	32.0	41.0
23	37.16204378628609	39.0	35.0	40.0	32.0	41.0
24	37.04502992403898	39.0	35.0	40.0	32.0	41.0
25	36.77777884344868	38.0	35.0	40.0	31.0	41.0
26	36.711066779201516	38.0	35.0	40.0	31.0	41.0
27	36.77644568914806	39.0	35.0	40.0	31.0	41.0
28	36.741940330954755	39.0	35.0	40.0	31.0	41.0
29	36.71963797028057	39.0	35.0	40.0	31.0	41.0
30	36.51491086728561	38.0	35.0	40.0	31.0	41.0
31	36.541695439781066	39.0	35.0	40.0	31.0	41.0
32	36.355766771528685	38.0	35.0	40.0	30.0	41.0
33	36.15714915982506	38.0	35.0	40.0	30.0	41.0
34	36.07241553492417	38.0	35.0	40.0	30.0	41.0
35	36.03463004168905	39.0	35.0	40.0	30.0	41.0
36	35.936772679608175	38.0	35.0	40.0	29.0	41.0
37	35.88165618046497	38.0	35.0	40.0	29.0	41.0
38	35.76534566101435	38.0	35.0	40.0	28.0	41.0
39	35.702390086702984	38.0	35.0	40.0	28.0	41.0
40	35.5430893373232	38.0	35.0	40.0	27.0	41.0
41	35.37513107752116	38.0	34.0	40.0	26.0	41.0
42	35.46064157651091	38.0	35.0	40.0	27.0	41.0
43	35.42612982429218	38.0	35.0	40.0	27.0	41.0
44	35.24092368091256	38.0	34.0	40.0	26.0	41.0
45	35.188156986112176	38.0	34.0	40.0	26.0	41.0
46	35.07736131358858	38.0	34.0	40.0	26.0	41.0
47	35.03695746694289	38.0	34.0	40.0	26.0	41.0
48	34.94947121409755	38.0	34.0	40.0	25.0	41.0
49	34.86170362413361	37.0	34.0	40.0	25.0	41.0
50	34.68021560653725	37.0	34.0	40.0	24.0	41.0
51	32.88007365917287	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	35.0
10	27.0
11	16.0
12	17.0
13	12.0
14	10.0
15	24.0
16	44.0
17	88.0
18	136.0
19	334.0
20	518.0
21	846.0
22	1234.0
23	1652.0
24	2337.0
25	3347.0
26	4334.0
27	4972.0
28	5208.0
29	5984.0
30	6943.0
31	8955.0
32	12084.0
33	16542.0
34	25824.0
35	28217.0
36	30535.0
37	44936.0
38	59648.0
39	47918.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.37741374459705	19.22012071919998	21.98457761068058	14.417887925522392
2	32.61912069362388	27.983452262206193	23.931558351876006	15.465868692293922
3	30.806414486303996	25.97157216297092	27.804419550372135	15.41759380035295
4	28.244328499450116	22.927056957978465	31.769354714954346	17.059259827617073
5	28.038760582112076	27.413105194506254	25.668815059208676	18.879319164172998
6	24.97858001483414	36.446584311619226	25.757052610041175	12.81778306350546
7	64.14358423489091	18.171180848615055	13.95943630271874	3.7257986137752885
8	74.5460241949922	8.923821581114606	12.072559400496177	4.457594823397018
9	69.09064170439142	7.723663009284125	13.099120182101846	10.086575104222614
10	41.76129824292181	24.49039617381519	20.039515077111947	13.708790506151052
11	33.78986674851019	24.91304125425203	25.013427453387553	16.283664543850225
12	31.569221719225553	20.497966699915597	29.121269086165885	18.811542494692958
13	24.808818639862913	24.27811452978337	31.981956060257293	18.93111077009642
14	18.97682805186833	29.75427760300775	29.251067802245583	22.017826542878336
15	17.722000562674236	26.62408245735185	38.014399345251796	17.639517634722115
16	20.532494437197883	24.16749788997161	33.82247627816568	21.477531394664826
17	20.372324100360622	24.31488017596358	31.942313102636895	23.370482621038903
18	20.45608583339727	26.18289470318934	30.93301618967237	22.428003273741016
19	23.814867387912734	27.215849510217655	28.26958489986956	20.699698202000054
20	26.135578915061767	25.73819023504437	29.72070896953886	18.405521880354996
21	24.559771349650887	27.56272538939615	28.387554668917364	19.489948592035603
22	20.993823371441724	23.94019028619658	29.854983503414413	25.211002838947287
23	21.875239775953347	27.791951200797975	29.414754853065293	20.91805417018338
24	23.421634824420064	24.616358474641295	29.81566024706514	22.146346453873498
25	21.45387350060104	28.17847003759687	28.987953656103738	21.379702805698354
26	20.522583697792783	27.328064656385077	30.02410547584337	22.12524616997877
27	22.74354842834855	27.94988362873731	28.57298140617407	20.73358653674007
28	18.910649888743958	27.000690554745642	32.94010076984066	21.148558786669735
29	22.029016087367964	24.962275250006392	31.66737000946316	21.341338653162484
30	22.7192511317425	26.692498529374152	29.886314227985373	20.70193611089798
31	23.53193176296069	24.530039131435586	27.667587406327527	24.270441699276198
32	24.256055142075244	26.759635796311926	29.438412747129085	19.545896314483745
33	23.532890866774085	26.194723650221235	26.93802910560372	23.334356377400955
34	21.771017161564234	24.511496457709917	31.523184736182515	22.194301644543337
35	22.639325813959434	23.883283459935036	30.55576868973631	22.921622036369214
36	21.772295966648763	25.6809637075117	29.08642164761247	23.460318678227065
37	22.917146218573365	26.519540141691607	30.638891020230695	19.924422619504334
38	22.830826875367656	26.313652523082432	26.44696795314458	24.40855264840533
39	23.033517481265505	24.20298473106729	30.588697920662934	22.17479986700427
40	24.077342131512314	22.872707741885982	31.629325558198417	21.420624568403284
41	18.32655566638533	26.589554720069568	30.163175528785903	24.9207140847592
42	20.442978081280852	25.686078927849813	31.247921941737637	22.62302104913169
43	23.439218394332336	24.84110846824727	27.74879152919512	23.970881608225277
44	22.534463797028057	25.70685951047341	28.848563901890074	22.910112790608455
45	21.205465612931278	24.992646870763956	28.262231770633523	25.539655745671247
46	23.24132330750147	27.27211693393693	27.476725747461572	22.009834011100025
47	21.010447837540603	24.369229392056063	33.30679812782936	21.313524642573977
48	21.83559681833295	24.275556919614314	29.2660937619888	24.622752500063942
49	19.942645591958875	23.524258932453517	32.38957518095092	24.14352029463669
50	20.30806414486304	23.65533645361774	31.031803882452238	25.004795519066985
51	20.119760096166143	24.14352029463669	29.085462543799075	26.65125706539809
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	448.0
2	647.0
3	2655.0
4	4663.0
5	3230.5
6	1798.0
7	1656.5
8	1515.0
9	1494.5
10	1474.0
11	1427.5
12	1381.0
13	1323.5
14	1266.0
15	1233.5
16	1201.0
17	1121.0
18	1041.0
19	1065.0
20	1089.0
21	1074.5
22	1060.0
23	1046.0
24	1032.0
25	1407.0
26	2021.5
27	2261.0
28	3110.5
29	3960.0
30	5189.0
31	6418.0
32	7113.0
33	7808.0
34	8186.0
35	8564.0
36	8701.0
37	8838.0
38	9799.5
39	10761.0
40	12434.0
41	14107.0
42	15923.5
43	17740.0
44	18867.0
45	19994.0
46	28511.5
47	37029.0
48	30963.0
49	24897.0
50	24514.5
51	24132.0
52	21736.0
53	19340.0
54	17674.0
55	16008.0
56	14928.5
57	13849.0
58	13932.5
59	14016.0
60	13983.5
61	13951.0
62	12314.0
63	10677.0
64	8883.5
65	7090.0
66	5695.0
67	4300.0
68	3457.5
69	2615.0
70	2178.5
71	1742.0
72	1488.5
73	1235.0
74	907.5
75	473.0
76	366.0
77	277.0
78	188.0
79	121.0
80	54.0
81	41.0
82	28.0
83	26.5
84	25.0
85	17.0
86	9.0
87	6.0
88	3.0
89	3.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	312792.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.17726796081741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.33941755537326	23.488772091357834
2	8.579778506972929	5.349881071127139
3	3.3193191140278917	3.1046190439653185
4	2.011894995898277	2.5090155758459294
5	1.3925348646431501	2.1707716309880047
6	0.9515996718621821	1.7800966776643903
7	0.7475389663658736	1.6314355865878922
8	0.5527071369975389	1.3785518811222794
9	0.4583675143560295	1.286158213765058
>10	6.38740771123872	45.379677229596666
>50	0.20303527481542247	3.8514412133302645
>100	0.04716981132075472	2.815289393590629
>500	0.004101722723543888	0.7637663367349549
>1k	0.005127153404429861	4.4905240543236395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	3940	1.2596230082610809	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	3317	1.0604491163456866	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	2895	0.9255351799278755	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2534	0.8101230210491316	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1360	0.4347937287398655	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	658	0.21036343640502314	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	648	0.2071664236937006	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	567	0.18127062073198802	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	516	0.16496585590424306	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	482	0.15409601268574644	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	439	0.14034885802705951	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	421	0.13459423514667895	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	360	0.11509245760761146	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	360	0.11509245760761146	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	354	0.11317424998081793	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	348	0.11125604235402439	No Hit
GAATCTATCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	344	0.10997723726949539	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.19701271132254E-4	0.0	0.0	0.12915931353743063	0.0
2	3.19701271132254E-4	0.0	0.0	0.952709787974117	0.0
3	3.19701271132254E-4	0.0	0.0	1.1573186014987595	0.0
4	3.19701271132254E-4	0.0	0.0	1.5620604107521932	0.0
5	3.19701271132254E-4	0.0	0.0	3.2037264380163175	0.0
6	3.19701271132254E-4	0.0	0.0	3.813396762065526	0.0
7	3.19701271132254E-4	0.0	0.0	4.359126831888283	0.0
8	3.19701271132254E-4	0.0	0.0	5.115540039387197	0.0
9	3.19701271132254E-4	0.0	0.0	5.358513005447709	0.0
10	3.19701271132254E-4	0.0	0.0	7.324675822911072	0.0
11	3.19701271132254E-4	0.0	0.0	8.064464564311107	0.0
12	3.19701271132254E-4	0.0	0.0	9.88164658942684	0.0
13	3.19701271132254E-4	0.0	0.0	10.261131998260826	0.0
14	3.19701271132254E-4	0.0	0.0	10.45742857873603	0.0
15	3.19701271132254E-4	0.0	0.0	10.858653674007007	0.0
16	3.19701271132254E-4	0.0	0.0	11.340123788332182	0.0
17	3.19701271132254E-4	0.0	0.0	11.87306580730965	0.0
18	3.19701271132254E-4	0.0	0.0	12.468988976700171	0.0
19	3.19701271132254E-4	0.0	0.0	12.862541241463976	0.0
20	3.19701271132254E-4	0.0	0.0	13.210056523184736	0.0
21	3.19701271132254E-4	0.0	0.0	13.660835315481215	0.0
22	3.19701271132254E-4	0.0	0.0	14.110335302693164	0.0
23	3.19701271132254E-4	0.0	0.0	14.545768433975294	0.0
24	3.19701271132254E-4	0.0	0.0	14.92397503772475	0.0
25	3.19701271132254E-4	0.0	0.0	15.217460804624158	0.0
26	3.19701271132254E-4	0.0	0.0	15.496560014322617	0.0
27	3.19701271132254E-4	0.0	0.0	15.781094145630323	0.0
28	3.19701271132254E-4	0.0	0.0	16.080654236681244	0.0
29	3.19701271132254E-4	0.0	0.0	16.390125067137266	0.0
30	3.19701271132254E-4	0.0	0.0	16.73668124504463	0.0
31	3.19701271132254E-4	0.0	0.0	17.054784009821223	0.0
32	3.19701271132254E-4	0.0	0.0	17.407734213151233	0.0
33	3.19701271132254E-4	0.0	0.0	17.72647638047009	0.0
34	3.19701271132254E-4	0.0	0.0	18.048415560500267	0.0
35	3.19701271132254E-4	0.0	0.0	18.382183687562343	0.0
36	3.19701271132254E-4	0.0	0.0	18.708918386659505	0.0
37	3.19701271132254E-4	0.0	0.0	19.016471009488733	0.0
38	9.59103813396762E-4	0.0	0.0	19.34064809841684	0.0
39	9.59103813396762E-4	0.0	0.0	19.665144888616076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGT	20	7.025405E-4	45.0	26
CTCACGA	35	1.2081364E-7	45.0	24
CGTATTC	20	7.025405E-4	45.0	38
CGACGTT	20	7.025405E-4	45.0	28
GAGTCGT	25	3.884273E-5	45.0	30
TTTCGAC	25	3.884273E-5	45.0	25
CGCATCG	25	3.884273E-5	45.0	21
AGGGTGC	20	7.025405E-4	45.0	7
CGACAGT	20	7.025405E-4	45.0	44
CGACAAT	20	7.025405E-4	45.0	20
TCCTATG	20	7.025405E-4	45.0	31
ACGGGTA	25	3.884273E-5	45.0	5
CATTATG	20	7.025405E-4	45.0	42
CGTTATG	25	3.884273E-5	45.0	26
GCCGATT	25	3.884273E-5	45.0	9
CTACGAA	20	7.025405E-4	45.0	11
AACGCTC	20	7.025405E-4	45.0	23
GTCGTAG	25	3.884273E-5	45.0	1
CGAATAT	25	3.884273E-5	45.0	14
TCCCGGT	20	7.025405E-4	45.0	17
>>END_MODULE