FastQCFastQC Report
Sat 18 Jun 2016
SRR3551415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125439
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.33009341243728No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC16810.14936393709476925No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC14860.132037364974912No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG14730.13088226016692153No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC14180.1259952782869618No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13330.11844266992702404No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG207.033222E-445.0000041
TATCGAA207.033222E-445.0000043
ATCGCTA207.033222E-445.00000424
GCTACGA2250.041.010
TACGAAT2350.039.25531812
CGAATAT2550.038.8235314
ACGTCGA356.248172E-638.57142614
CGATAGT356.248172E-638.57142611
CGTACTA301.1399067E-437.50000444
ACGTACT301.1399067E-437.50000443
CGCTATT900.037.544
TACGCGG850.037.0588262
ATTAGCG552.748493E-936.818181
CGTTTTT17550.036.0256421
TGATCGC250.002107089636.00000422
TCGTTAC250.002107089636.00000435
GATCGCT250.002107089636.00000423
ATATCGT250.002107089636.00000416
ACTCGCA250.002107089636.00000410
TATCGTT250.002107089636.00000417
Produced by FastQC (version 0.11.5)