##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1125439
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136002040092798	31.0	31.0	33.0	28.0	34.0
2	31.17822112082485	31.0	31.0	34.0	28.0	34.0
3	30.982040785862228	31.0	31.0	34.0	28.0	34.0
4	34.95666668739932	35.0	35.0	37.0	32.0	37.0
5	35.18372652804817	35.0	35.0	37.0	33.0	37.0
6	35.14463333863497	36.0	35.0	37.0	32.0	37.0
7	31.12856049950286	35.0	35.0	37.0	0.0	37.0
8	33.11760210904367	36.0	35.0	37.0	17.0	37.0
9	36.32655079484539	39.0	35.0	39.0	32.0	39.0
10	36.08284233974476	37.0	35.0	39.0	32.0	39.0
11	36.364536860727235	38.0	35.0	39.0	32.0	39.0
12	36.42336368297171	38.0	35.0	39.0	32.0	39.0
13	36.39636088672953	38.0	35.0	39.0	32.0	39.0
14	37.33307180575758	39.0	36.0	40.0	32.0	41.0
15	37.553709263673994	39.0	36.0	41.0	32.0	41.0
16	37.49020071278852	39.0	36.0	41.0	32.0	41.0
17	37.428019643890075	39.0	36.0	41.0	32.0	41.0
18	37.37021197950311	39.0	36.0	40.0	32.0	41.0
19	37.18837626917141	39.0	36.0	40.0	32.0	41.0
20	37.191543033429625	39.0	35.0	40.0	32.0	41.0
21	37.17865561794109	39.0	35.0	40.0	32.0	41.0
22	37.29664690845084	39.0	35.0	40.0	32.0	41.0
23	37.368636594253445	39.0	35.0	40.0	32.0	41.0
24	37.312879685171744	39.0	35.0	41.0	32.0	41.0
25	36.9800913243632	39.0	35.0	40.0	31.0	41.0
26	36.983237652151736	39.0	35.0	40.0	31.0	41.0
27	37.00487543083188	39.0	35.0	40.0	31.0	41.0
28	36.9782458222969	39.0	35.0	40.0	31.0	41.0
29	36.98182398157519	39.0	35.0	40.0	31.0	41.0
30	36.74873449382863	39.0	35.0	40.0	31.0	41.0
31	36.742166390181964	39.0	35.0	40.0	31.0	41.0
32	36.645349059344845	39.0	35.0	40.0	31.0	41.0
33	36.55582665964126	39.0	35.0	40.0	30.0	41.0
34	36.49421159209873	39.0	35.0	40.0	30.0	41.0
35	36.443019124092906	39.0	35.0	40.0	30.0	41.0
36	36.37326945307564	39.0	35.0	40.0	30.0	41.0
37	36.31906749277393	39.0	35.0	40.0	30.0	41.0
38	36.14690089822727	39.0	35.0	40.0	30.0	41.0
39	36.100049847215175	39.0	35.0	40.0	29.0	41.0
40	35.9690334171821	38.0	35.0	40.0	29.0	41.0
41	35.78446188553978	38.0	35.0	40.0	28.0	41.0
42	35.82488877673512	38.0	35.0	40.0	29.0	41.0
43	35.778705020885184	38.0	35.0	40.0	29.0	41.0
44	35.58578830127621	38.0	34.0	40.0	28.0	41.0
45	35.519811380270276	38.0	34.0	40.0	28.0	41.0
46	35.420379069856295	38.0	34.0	40.0	27.0	41.0
47	35.37157322609222	38.0	34.0	40.0	27.0	41.0
48	35.27807015751187	38.0	34.0	40.0	27.0	41.0
49	35.1766519553703	38.0	34.0	40.0	27.0	41.0
50	35.01981360162568	37.0	34.0	40.0	26.0	41.0
51	33.397584409283844	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	50.0
9	92.0
10	103.0
11	83.0
12	58.0
13	39.0
14	71.0
15	123.0
16	178.0
17	329.0
18	582.0
19	982.0
20	1620.0
21	2524.0
22	3724.0
23	5070.0
24	7229.0
25	9773.0
26	12371.0
27	14464.0
28	17212.0
29	20440.0
30	25421.0
31	32249.0
32	41783.0
33	58209.0
34	85742.0
35	92347.0
36	112511.0
37	169443.0
38	225295.0
39	185302.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8346280873508	21.431192627943407	23.69813024073273	11.036049043973064
2	33.68658807807442	23.676716374676904	24.569079265957548	18.067616281291123
3	34.0046861713518	23.16162848452915	25.95467191025013	16.879013433868916
4	30.069155236312227	25.85328924979497	24.37493280399915	19.70262270989365
5	26.11896335563278	29.624351031019895	24.267952327936033	19.988733285411293
6	26.874224191626556	34.912420842000316	24.337791741711456	13.875563224661667
7	69.95474654779157	18.037494702067374	8.209507578820354	3.7982511713207026
8	80.4368784092252	6.493199542578496	8.620014056736972	4.449907991459333
9	73.34577884718763	8.686743572952421	11.347483071050497	6.619994508809452
10	41.47972480072221	26.351317130470864	18.006306872251628	14.1626511965553
11	31.901862295513126	25.808062453851342	24.377243013615132	17.9128322370204
12	29.47720844932511	23.483014183798502	28.137464580488146	18.902312786388244
13	26.0198909047936	25.047736927545607	29.201049545999386	19.731322621661416
14	20.673088457037654	27.44306888245387	30.346646952877943	21.537195707630534
15	20.859593456420118	26.379039645862633	32.70368274069052	20.057684157026724
16	24.35538487647931	26.33621191375099	29.819208326706292	19.48919488306341
17	24.8426613970193	25.51164478927778	27.727313519435526	21.918380294267394
18	25.9831941135859	25.36103689315902	28.425085677677774	20.2306833155773
19	25.45406725731026	27.64290201423622	26.083599377665074	20.81943135078845
20	26.650578129956397	27.259051801119384	27.093960667792743	18.99640940113147
21	25.355261369119077	26.782260078067317	28.516960937021018	19.34551761579259
22	24.45507930683049	24.094686606737458	29.242722173303036	22.20751191312901
23	23.327075034719787	26.768754237235427	28.947281905105477	20.956888822939316
24	23.199036109464842	25.26569631939181	29.670644077555515	21.864623493587835
25	23.774900283356097	26.37770683262265	27.258874092687385	22.58851879133387
26	22.25131704161665	27.406994070758167	27.147717468472305	23.19397141915288
27	22.72544313818874	26.87253596152257	28.55436856195671	21.847652338331976
28	20.56904017010251	27.757079681795283	29.169595153535642	22.504284994566564
29	22.098043519017914	26.590068408860894	28.740340436043176	22.57154763607801
30	23.184108601176963	25.868394466514843	28.86509175530615	22.08240517700204
31	23.123065754785465	26.308844815223214	28.44880975334958	22.11927967664174
32	24.269551703824018	26.54386421654128	27.606560639892525	21.58002343974218
33	23.453514584086744	26.500681067565633	27.87721058182629	22.16859376652133
34	20.834092296428327	25.8861653097147	29.861502933521944	23.418239460335034
35	21.503075688686817	26.616102694148687	28.236625885543333	23.644195731621174
36	22.411698901495328	27.93798686556979	27.614735227764452	22.035579005170426
37	22.12496634646569	27.505533396301356	28.687027906443618	21.682472350789336
38	21.958631254115062	27.31431912347093	28.31401790767869	22.413031714735315
39	22.238610888728754	25.73164782809197	27.653386811724133	24.376354471455137
40	23.42605863134297	24.70271600682045	29.165152442735682	22.706072919100905
41	20.20020631948955	25.346642510167143	29.010279544248956	25.442871626094348
42	21.06573523753842	25.332692398255258	29.774692364490658	23.826879999715665
43	21.682650059221334	25.542743764877528	30.11562599127985	22.658980184621292
44	21.87875131393172	25.3758755472309	28.743361479387154	24.002011659450222
45	21.83130316258811	25.018770453129846	28.10174518565644	25.048181198625603
46	20.85906033112412	26.40187517937445	27.84069149905059	24.898372990450838
47	20.523813374158884	26.058631342969278	30.312526933934226	23.105028348937616
48	20.96328632649126	25.857287689514934	29.90050993434562	23.27891604964818
49	21.533641538990565	24.857588905307175	30.146280695799593	23.46248885990267
50	19.571296178646733	26.117008562880795	29.701298782075263	24.610396476397213
51	19.564720966662787	25.22588963062414	28.2888721645509	26.920517238162173
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	297.0
1	1003.0
2	1709.0
3	4186.0
4	6663.0
5	4555.0
6	2447.0
7	2332.0
8	2217.0
9	2170.5
10	2124.0
11	2127.5
12	2131.0
13	2102.5
14	2074.0
15	2130.0
16	2186.0
17	2241.5
18	2297.0
19	2508.0
20	2719.0
21	3567.0
22	4415.0
23	4905.0
24	5395.0
25	6525.0
26	9462.5
27	11270.0
28	13215.0
29	15160.0
30	18224.0
31	21288.0
32	24161.5
33	27035.0
34	31934.0
35	36833.0
36	39090.5
37	41348.0
38	44040.0
39	46732.0
40	52011.0
41	57290.0
42	64023.0
43	70756.0
44	78705.5
45	86655.0
46	88532.5
47	90410.0
48	89965.0
49	89520.0
50	83563.5
51	77607.0
52	71766.5
53	65926.0
54	63793.0
55	61660.0
56	60147.5
57	58635.0
58	56899.0
59	55163.0
60	52031.5
61	48900.0
62	43774.5
63	38649.0
64	33006.0
65	27363.0
66	23322.5
67	19282.0
68	16065.5
69	12849.0
70	10743.0
71	8637.0
72	7058.0
73	5479.0
74	4363.5
75	2344.0
76	1440.0
77	1236.5
78	1033.0
79	747.5
80	462.0
81	344.5
82	227.0
83	176.0
84	125.0
85	96.5
86	68.0
87	46.5
88	25.0
89	19.5
90	14.0
91	11.0
92	8.0
93	8.0
94	8.0
95	5.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1125439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.10826957385714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51874031432452	22.135220521140813
2	9.061506357858491	5.456525523352477
3	3.878513033438933	3.5032594786949334
4	2.3651471311863874	2.8484194963037845
5	1.5953492578001203	2.4016602759149466
6	1.0984996574726058	1.984435428838497
7	0.8378945431915968	1.765928834661354
8	0.6477544196339851	1.560221174719792
9	0.5091961002994246	1.3797912108394685
>10	5.9052945728467465	43.75087144457474
>50	0.5374839227369431	9.74014596806225
>100	0.041433497124223694	2.1944499026666477
>500	0.0014487236754906815	0.3102152197694047
>1k	0.0017384684105888181	0.9688555204609135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3715	0.33009341243728	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1681	0.14936393709476925	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC	1486	0.132037364974912	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	1473	0.13088226016692153	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	1418	0.1259952782869618	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1333	0.11844266992702404	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.885421599926784E-5	0.0	0.0	0.0781917100793557	0.0
2	8.885421599926784E-5	0.0	0.0	0.4421385788123568	0.0
3	8.885421599926784E-5	0.0	0.0	0.5924799122831179	0.0
4	8.885421599926784E-5	0.0	0.0	0.8389615074650869	0.0
5	8.885421599926784E-5	0.0	0.0	1.561523991971133	0.0
6	8.885421599926784E-5	0.0	0.0	2.0376937355112092	0.0
7	8.885421599926784E-5	0.0	0.0	2.4576187603237494	0.0
8	8.885421599926784E-5	0.0	0.0	3.1878227074057324	0.0
9	8.885421599926784E-5	0.0	0.0	3.4632707770034625	0.0
10	8.885421599926784E-5	0.0	0.0	4.15784418346974	0.0
11	8.885421599926784E-5	0.0	0.0	4.92963190363938	0.0
12	8.885421599926784E-5	0.0	0.0	5.681072008345188	0.0
13	8.885421599926784E-5	0.0	0.0	5.973757795846776	0.0
14	8.885421599926784E-5	0.0	0.0	6.117968188413588	0.0
15	8.885421599926784E-5	0.0	0.0	6.358496551123606	0.0
16	8.885421599926784E-5	0.0	0.0	6.805077840735926	0.0
17	8.885421599926784E-5	0.0	0.0	7.322209377851665	0.0
18	8.885421599926784E-5	0.0	0.0	7.8849231277750285	0.0
19	8.885421599926784E-5	0.0	0.0	8.225412483484222	0.0
20	8.885421599926784E-5	0.0	0.0	8.570255695777382	0.0
21	8.885421599926784E-5	0.0	0.0	9.002442602397819	0.0
22	1.7770843199853569E-4	0.0	0.0	9.471948279737951	0.0
23	1.7770843199853569E-4	0.0	0.0	9.89302840935848	0.0
24	1.7770843199853569E-4	0.0	0.0	10.252177150427523	0.0
25	1.7770843199853569E-4	0.0	0.0	10.584580772480782	0.0
26	2.665626479978035E-4	0.0	0.0	10.898858134470194	0.0
27	2.665626479978035E-4	0.0	0.0	11.243345929899355	0.0
28	2.665626479978035E-4	0.0	0.0	11.58125851334457	0.0
29	2.665626479978035E-4	0.0	0.0	11.93925214960562	0.0
30	2.665626479978035E-4	0.0	0.0	12.408757826945752	0.0
31	2.665626479978035E-4	0.0	0.0	12.78985355936661	0.0
32	2.665626479978035E-4	0.0	0.0	13.168816790603488	0.0
33	2.665626479978035E-4	0.0	0.0	13.520057506448595	0.0
34	2.665626479978035E-4	0.0	0.0	13.89191240040553	0.0
35	2.665626479978035E-4	0.0	0.0	14.295932520554201	0.0
36	2.665626479978035E-4	0.0	0.0	14.664055537439168	0.0
37	2.665626479978035E-4	0.0	0.0	15.04239678916405	0.0
38	2.665626479978035E-4	0.0	0.0	15.47467255000049	0.0
39	4.442710799963392E-4	0.0	0.0	16.112734675091232	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	20	7.033222E-4	45.000004	1
TATCGAA	20	7.033222E-4	45.000004	3
ATCGCTA	20	7.033222E-4	45.000004	24
GCTACGA	225	0.0	41.0	10
TACGAAT	235	0.0	39.255318	12
CGAATAT	255	0.0	38.82353	14
ACGTCGA	35	6.248172E-6	38.571426	14
CGATAGT	35	6.248172E-6	38.571426	11
CGTACTA	30	1.1399067E-4	37.500004	44
ACGTACT	30	1.1399067E-4	37.500004	43
CGCTATT	90	0.0	37.5	44
TACGCGG	85	0.0	37.058826	2
ATTAGCG	55	2.748493E-9	36.81818	1
CGTTTTT	1755	0.0	36.025642	1
TGATCGC	25	0.0021070896	36.000004	22
TCGTTAC	25	0.0021070896	36.000004	35
GATCGCT	25	0.0021070896	36.000004	23
ATATCGT	25	0.0021070896	36.000004	16
ACTCGCA	25	0.0021070896	36.000004	10
TATCGTT	25	0.0021070896	36.000004	17
>>END_MODULE