FastQCFastQC Report
Sat 18 Jun 2016
SRR3551414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727342
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.2456890981134047No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC10170.13982418174668862TruSeq Adapter, Index 15 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC8470.11645140800338769No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC7890.10847716754979089No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG7830.10765224612355673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA453.8380676E-1045.031
CGGTATG253.889387E-545.02
CGGGTAC403.4558798E-739.3756
TACGGGT403.4558798E-739.3754
CGATTCG356.2451254E-638.57142610
CACGACC356.2451254E-638.57142627
CGATATG356.2451254E-638.57142610
TCGAACG356.2451254E-638.5714261
GCATATC1250.037.80000328
CACAACG1550.037.74193612
TTAGCCG301.13951E-437.4999961
CTAAGCG301.13951E-437.4999961
ATCGTTC301.13951E-437.49999635
ATATCGT301.13951E-437.49999633
CGTTTTT9350.036.577541
CGAATAT751.8189894E-1236.00000414
AATCCGT250.002106600736.044
GCAACGT250.002106600736.015
TTATCCG250.002106600736.01
ATCGTTG250.002106600736.023
Produced by FastQC (version 0.11.5)