FastQCFastQC Report
Sat 18 Jun 2016
SRR3551413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592104
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG31820.5374055909097051No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC29390.4963655033575183No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC23980.40499641954791726No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.3470674070771351No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC10600.17902260413711105No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9330.15757366949049492No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA8900.1503114317754989No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT8040.13578695634550686No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6300.10640022698715092No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC207.0305116E-445.00000427
TACGGAT207.0305116E-445.00000418
TACGGAC207.0305116E-445.00000434
GTATGCG207.0305116E-445.0000041
TGCGAAA207.0305116E-445.0000041
AGCGCAT207.0305116E-445.00000433
CGTCAGT207.0305116E-445.00000420
GACCGAC253.888506E-545.012
ACGGGTC302.1637134E-644.9999965
CGAATAT1150.041.08695614
GCTACGA1150.041.08695610
ACAACGA1100.040.90909213
CCGATAT451.9255822E-840.00000411
TACGGCT3300.039.5454527
TATACGG403.4544428E-739.3750041
TACGGGT356.243159E-638.5714264
CGTTTTT9500.037.8947371
CGGTAGT1250.037.80000312
TTACACG2450.037.6530634
CGAACGG601.546141E-1037.4999962