##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592104
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28543972004918	31.0	31.0	33.0	30.0	34.0
2	31.31937970356559	31.0	31.0	34.0	30.0	34.0
3	31.10918858849121	31.0	31.0	34.0	28.0	34.0
4	35.093865266912566	35.0	35.0	37.0	32.0	37.0
5	35.29494818477835	35.0	35.0	37.0	33.0	37.0
6	35.25706294840096	37.0	35.0	37.0	33.0	37.0
7	30.7983192141921	35.0	35.0	37.0	0.0	37.0
8	32.96161654033751	36.0	35.0	37.0	17.0	37.0
9	36.310322173131745	39.0	35.0	39.0	32.0	39.0
10	36.20041073865402	37.0	35.0	39.0	32.0	39.0
11	36.48704281680245	38.0	35.0	39.0	32.0	39.0
12	36.57331144528664	38.0	35.0	39.0	32.0	39.0
13	36.51630625700891	39.0	35.0	39.0	32.0	39.0
14	37.6508096550606	39.0	36.0	41.0	33.0	41.0
15	37.79665734398011	40.0	37.0	41.0	33.0	41.0
16	37.75984962101253	39.0	36.0	41.0	33.0	41.0
17	37.6355606447516	39.0	36.0	41.0	33.0	41.0
18	37.5727997108616	39.0	36.0	41.0	33.0	41.0
19	37.40382601705106	39.0	36.0	40.0	32.0	41.0
20	37.39043985516058	39.0	36.0	40.0	32.0	41.0
21	37.415447286287545	39.0	36.0	40.0	32.0	41.0
22	37.496595192736415	39.0	36.0	41.0	33.0	41.0
23	37.55235060057017	39.0	36.0	41.0	33.0	41.0
24	37.48459223379677	39.0	36.0	41.0	33.0	41.0
25	37.1870955102482	39.0	35.0	40.0	32.0	41.0
26	37.14956831907908	39.0	35.0	40.0	32.0	41.0
27	37.20196451974653	39.0	35.0	40.0	32.0	41.0
28	37.15692513477362	39.0	35.0	41.0	32.0	41.0
29	37.15736086903652	39.0	35.0	41.0	32.0	41.0
30	36.96833326577763	39.0	35.0	40.0	31.0	41.0
31	36.97927728912488	39.0	35.0	40.0	31.0	41.0
32	36.8730071068596	39.0	35.0	40.0	31.0	41.0
33	36.772305203139986	39.0	35.0	40.0	31.0	41.0
34	36.71793299825706	39.0	35.0	40.0	31.0	41.0
35	36.68488981665383	39.0	35.0	41.0	31.0	41.0
36	36.57593936200397	39.0	35.0	40.0	30.0	41.0
37	36.53156877845784	39.0	35.0	40.0	30.0	41.0
38	36.423986326726386	39.0	35.0	40.0	30.0	41.0
39	36.36648122627106	39.0	35.0	40.0	30.0	41.0
40	36.2370326834475	39.0	35.0	40.0	30.0	41.0
41	36.03128166673422	38.0	35.0	40.0	29.0	41.0
42	36.05968039398484	38.0	35.0	40.0	30.0	41.0
43	35.97451461229784	38.0	35.0	40.0	29.0	41.0
44	35.827712699120426	38.0	35.0	40.0	29.0	41.0
45	35.76442989745045	38.0	35.0	40.0	29.0	41.0
46	35.62420453163634	38.0	35.0	40.0	28.0	41.0
47	35.56824307891857	38.0	34.0	40.0	28.0	41.0
48	35.45840764460298	38.0	34.0	40.0	28.0	41.0
49	35.327108075608336	38.0	34.0	40.0	27.0	41.0
50	35.17892971505006	38.0	34.0	40.0	27.0	41.0
51	33.48377818761569	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	29.0
9	43.0
10	58.0
11	54.0
12	28.0
13	24.0
14	32.0
15	57.0
16	69.0
17	112.0
18	268.0
19	459.0
20	785.0
21	1190.0
22	1799.0
23	2361.0
24	3214.0
25	4436.0
26	5713.0
27	6801.0
28	7990.0
29	9712.0
30	12044.0
31	15420.0
32	20280.0
33	29095.0
34	45065.0
35	50206.0
36	60522.0
37	91198.0
38	122104.0
39	100934.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9002269871509	20.121127369516167	24.102184751327467	11.876460892005458
2	32.51557158877495	25.997797684190616	24.544674584194667	16.94195614283977
3	32.89084350046613	25.26667612446462	25.813539513328738	16.028940861740505
4	30.33149581830219	25.48521881291125	25.99999324443003	18.18329212435653
5	26.823159444962368	30.208206666396443	23.500432356477916	19.468201532163267
6	26.59870563279424	36.29632632105171	23.516307945901396	13.588660100252659
7	67.6208909245673	19.46110818369746	9.268810884574332	3.649190007160904
8	79.39095158958561	7.996568170456541	8.508302595489981	4.104177644467864
9	72.75073297934146	9.147210625160444	10.421986678016024	7.6800697174820645
10	41.53459527380325	27.43352519152041	17.298988015618878	13.732891519057464
11	33.12306621809682	26.63670571386108	22.620181589720726	17.620046478321377
12	31.1958709956359	22.71222623052707	26.93699079891371	19.154911974923326
13	25.94054422871658	24.695830462216097	28.603589909880693	20.76003539918663
14	20.698897490981313	29.1818666990934	27.243355896937025	22.87587991298826
15	20.320585572804777	26.981577560698796	33.03135935578885	19.666477510707576
16	22.78349749368354	25.892410792698577	30.304980206179994	21.01911150743788
17	22.50550578952346	25.72453487900774	28.680096739761936	23.08986259170686
18	23.588930323051354	27.180529096239848	27.28844932646967	21.94209125423912
19	25.414285328253143	27.983935254617432	25.554463405077488	21.047316012051937
20	28.01821977220218	26.005397700403982	26.989684244659724	18.986698282734114
21	26.318518368394738	27.39062732222718	25.815903962817345	20.47495034656074
22	23.981766716657884	26.01755772634537	26.6497101860484	23.350965370948344
23	24.45229216488995	27.097604474889547	27.18458243822031	21.26552092200019
24	23.951704430302783	25.393343061354084	28.268344750246577	22.38660775809655
25	23.05540918487293	27.686014619053413	26.395194087525166	22.8633821085485
26	21.88618891275857	28.265642522259604	27.20468025887344	22.643488306108388
27	23.56410360342102	28.29367813762447	27.1030089308635	21.03920932809101
28	21.206916352532662	28.936977287773768	29.02665747909151	20.829448880602055
29	23.308067501655113	26.49770986178104	28.650878899652767	21.543343736911083
30	22.2994609055166	26.56695445394728	28.69664788618216	22.436936754353965
31	23.972308918703472	27.917055111939792	25.763379406320514	22.347256563036225
32	25.768446083796086	27.93343736911083	26.1687136043668	20.129402942726276
33	23.95981111426371	27.647338980989826	25.904233040141595	22.488616864604865
34	23.83922419034494	27.412245146122977	27.7258724818613	21.022658181670785
35	25.233573831624174	25.6252280004864	26.796306054341805	22.344892113547623
36	22.602279329307013	28.91654168862227	27.47608528231527	21.00509369975545
37	24.40297650412765	28.319687081999106	27.415285152608327	19.862051261264913
38	22.91675111129126	28.27155364598111	25.665085863294284	23.146609379433343
39	25.240667180089982	25.40550308729547	26.953204166835555	22.400625565778984
40	25.200978206531282	24.683332657776337	28.945928417980625	21.169760717711753
41	22.119762744382744	26.27899828408523	28.83699485225569	22.764244119276345
42	22.722190696229042	26.926857443962547	28.4448340156459	21.906117844162512
43	23.180556118519718	26.436403064326537	27.979544134138596	22.403496683015145
44	23.447570021482715	26.71507032548336	27.245044789428885	22.592314863605043
45	22.882635482955695	25.938686446975534	26.319362814640673	24.8593152554281
46	22.338812100576927	28.028015348654968	26.450251982759788	23.18292056800832
47	23.02146244578655	25.78111277748504	30.070055260562334	21.127369516166077
48	23.096111493926745	25.258738329752884	28.417473957277778	23.2276762190426
49	21.43322794644184	25.722677097266693	29.38672935835596	23.4573655979355
50	20.42141245456879	26.462412008701175	29.23168902760326	23.884486509126777
51	22.020793644359774	25.867921851566617	26.822146109467255	25.289138394606354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	331.0
1	771.5
2	1212.0
3	2672.5
4	4133.0
5	2887.0
6	1641.0
7	1501.5
8	1362.0
9	1341.0
10	1320.0
11	1307.5
12	1295.0
13	1273.5
14	1252.0
15	1260.5
16	1269.0
17	1292.0
18	1315.0
19	1334.0
20	1353.0
21	2127.5
22	2902.0
23	2884.5
24	2867.0
25	3523.0
26	5272.0
27	6365.0
28	7902.0
29	9439.0
30	11272.0
31	13105.0
32	13918.5
33	14732.0
34	16644.5
35	18557.0
36	18910.5
37	19264.0
38	20590.0
39	21916.0
40	25579.0
41	29242.0
42	30106.0
43	30970.0
44	34173.0
45	37376.0
46	44948.5
47	52521.0
48	47725.0
49	42929.0
50	41822.5
51	40716.0
52	37833.0
53	34950.0
54	32749.0
55	30548.0
56	30582.5
57	30617.0
58	31002.0
59	31387.0
60	31528.0
61	31669.0
62	28313.0
63	24957.0
64	20622.0
65	16287.0
66	12999.0
67	9711.0
68	8398.0
69	7085.0
70	5910.5
71	4736.0
72	3799.0
73	2862.0
74	2315.5
75	1376.0
76	983.0
77	753.0
78	523.0
79	378.0
80	233.0
81	182.0
82	131.0
83	84.0
84	37.0
85	29.5
86	22.0
87	20.0
88	18.0
89	14.5
90	11.0
91	8.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.936750065078915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08966719635954	21.581303491321336
2	8.705525480222747	5.212302809732089
3	3.756803701098711	3.3739948033006666
4	2.3287804845529605	2.7886447728998136
5	1.6753731031286407	2.50776129270589
6	1.2087464641851435	2.1711564474215104
7	0.9357950719791039	1.9610264227379677
8	0.7584329335556212	1.8164013738383389
9	0.5839361054031413	1.5733034317286478
>10	7.769661703986282	49.18953025016017
>50	0.13457084229563418	2.552630337826929
>100	0.045417659274775535	2.4097824113791497
>500	0.004485694743187708	0.9092916775174739
>1k	0.0028035592144923173	1.9528704774299965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG	3182	0.5374055909097051	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC	2939	0.4963655033575183	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	2398	0.40499641954791726	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2055	0.3470674070771351	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1060	0.17902260413711105	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	933	0.15757366949049492	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	890	0.1503114317754989	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT	804	0.13578695634550686	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	630	0.10640022698715092	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6888924918595383E-4	0.0	0.0	0.0734668233958899	0.0
2	1.6888924918595383E-4	0.0	0.0	0.4521165200707984	0.0
3	1.6888924918595383E-4	0.0	0.0	0.5843568021834002	0.0
4	1.6888924918595383E-4	0.0	0.0	0.8324551092375664	0.0
5	1.6888924918595383E-4	0.0	0.0	1.729932579411725	0.0
6	1.6888924918595383E-4	0.0	0.0	2.1855957737154283	0.0
7	1.6888924918595383E-4	0.0	0.0	2.549045437963601	0.0
8	1.6888924918595383E-4	0.0	0.0	3.0589220812559956	0.0
9	1.6888924918595383E-4	0.0	0.0	3.236086903652061	0.0
10	1.6888924918595383E-4	0.0	0.0	4.235573480334536	0.0
11	1.6888924918595383E-4	0.0	0.0	4.801352465107481	0.0
12	1.6888924918595383E-4	0.0	0.0	5.832927999135287	0.0
13	1.6888924918595383E-4	0.0	0.0	6.108386364557577	0.0
14	1.6888924918595383E-4	0.0	0.0	6.242653317660411	0.0
15	1.6888924918595383E-4	0.0	0.0	6.484164943996325	0.0
16	1.6888924918595383E-4	0.0	0.0	6.867036871900882	0.0
17	1.6888924918595383E-4	0.0	0.0	7.275073297934147	0.0
18	1.6888924918595383E-4	0.0	0.0	7.718238687798089	0.0
19	1.6888924918595383E-4	0.0	0.0	8.003999297420723	0.0
20	1.6888924918595383E-4	0.0	0.0	8.284355451069407	0.0
21	1.6888924918595383E-4	0.0	0.0	8.606933917014578	0.0
22	1.6888924918595383E-4	0.0	0.0	8.984063610446814	0.0
23	1.6888924918595383E-4	0.0	0.0	9.32150433032035	0.0
24	1.6888924918595383E-4	0.0	0.0	9.610980503425074	0.0
25	1.6888924918595383E-4	0.0	0.0	9.859247699728426	0.0
26	1.6888924918595383E-4	0.0	0.0	10.107683785280964	0.0
27	1.6888924918595383E-4	0.0	0.0	10.354599867590828	0.0
28	1.6888924918595383E-4	0.0	0.0	10.60286706389418	0.0
29	1.6888924918595383E-4	0.0	0.0	10.864645400132408	0.0
30	3.3777849837190766E-4	0.0	0.0	11.172699390647589	0.0
31	3.3777849837190766E-4	0.0	0.0	11.452379987299528	0.0
32	3.3777849837190766E-4	0.0	0.0	11.739829489414022	0.0
33	3.3777849837190766E-4	0.0	0.0	12.032345669004094	0.0
34	5.066677475578615E-4	0.0	0.0	12.327395187331955	0.0
35	5.066677475578615E-4	0.0	0.0	12.608595787226568	0.0
36	5.066677475578615E-4	0.0	0.0	12.91073865402024	0.0
37	5.066677475578615E-4	0.0	0.0	13.205957061597287	0.0
38	5.066677475578615E-4	0.0	0.0	13.506242146649912	0.0
39	5.066677475578615E-4	0.0	0.0	13.816153918906139	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGC	20	7.0305116E-4	45.000004	27
TACGGAT	20	7.0305116E-4	45.000004	18
TACGGAC	20	7.0305116E-4	45.000004	34
GTATGCG	20	7.0305116E-4	45.000004	1
TGCGAAA	20	7.0305116E-4	45.000004	1
AGCGCAT	20	7.0305116E-4	45.000004	33
CGTCAGT	20	7.0305116E-4	45.000004	20
GACCGAC	25	3.888506E-5	45.0	12
ACGGGTC	30	2.1637134E-6	44.999996	5
CGAATAT	115	0.0	41.086956	14
GCTACGA	115	0.0	41.086956	10
ACAACGA	110	0.0	40.909092	13
CCGATAT	45	1.9255822E-8	40.000004	11
TACGGCT	330	0.0	39.545452	7
TATACGG	40	3.4544428E-7	39.375004	1
TACGGGT	35	6.243159E-6	38.571426	4
CGTTTTT	950	0.0	37.894737	1
CGGTAGT	125	0.0	37.800003	12
TTACACG	245	0.0	37.65306	34
CGAACGG	60	1.546141E-10	37.499996	2
>>END_MODULE