##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201625
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98240297582145	33.0	31.0	34.0	30.0	34.0
2	32.09740855548667	33.0	31.0	34.0	30.0	34.0
3	31.890881587104772	33.0	31.0	34.0	30.0	34.0
4	35.69835585864848	37.0	35.0	37.0	33.0	37.0
5	35.812195908245506	37.0	35.0	37.0	35.0	37.0
6	35.8054457532548	37.0	35.0	37.0	35.0	37.0
7	26.875848729076257	35.0	10.0	37.0	0.0	37.0
8	31.212548047117174	36.0	19.0	37.0	17.0	37.0
9	36.00572349659021	39.0	32.0	39.0	32.0	39.0
10	36.68586236825791	38.0	35.0	39.0	33.0	39.0
11	37.04420086794792	39.0	37.0	39.0	34.0	39.0
12	37.109787972721634	39.0	37.0	39.0	34.0	39.0
13	37.0814730316181	39.0	37.0	39.0	33.0	39.0
14	38.316012399256046	40.0	38.0	41.0	34.0	41.0
15	38.37687786732796	40.0	38.0	41.0	34.0	41.0
16	38.32029758214507	40.0	37.0	41.0	34.0	41.0
17	38.222169869807814	40.0	37.0	41.0	34.0	41.0
18	38.10188716676999	40.0	37.0	41.0	33.0	41.0
19	37.9438611283323	40.0	37.0	41.0	33.0	41.0
20	37.96628890266584	40.0	36.0	41.0	34.0	41.0
21	37.89821698698078	40.0	36.0	41.0	33.0	41.0
22	37.901867327960325	40.0	36.0	41.0	33.0	41.0
23	37.81828642281463	40.0	36.0	41.0	33.0	41.0
24	37.74605827650341	40.0	36.0	41.0	33.0	41.0
25	37.64885802851829	40.0	36.0	41.0	33.0	41.0
26	37.51981649101054	39.0	35.0	41.0	33.0	41.0
27	37.39227278363298	39.0	35.0	41.0	33.0	41.0
28	37.36379417234966	39.0	35.0	41.0	33.0	41.0
29	37.31953874767514	39.0	35.0	41.0	32.0	41.0
30	37.21149163050217	39.0	35.0	41.0	32.0	41.0
31	37.1113899566026	39.0	35.0	41.0	32.0	41.0
32	36.92619962802232	39.0	35.0	41.0	31.0	41.0
33	36.78499690018599	39.0	35.0	41.0	31.0	41.0
34	36.59803099814011	39.0	35.0	41.0	30.0	41.0
35	36.44655176689399	39.0	35.0	41.0	30.0	41.0
36	36.2598884066956	39.0	35.0	41.0	30.0	41.0
37	36.19859640421575	39.0	35.0	41.0	30.0	41.0
38	36.063231246125234	39.0	35.0	41.0	29.0	41.0
39	36.020314941103535	39.0	35.0	41.0	29.0	41.0
40	35.84739491630502	39.0	35.0	40.0	28.0	41.0
41	35.76967141971482	39.0	35.0	40.0	28.0	41.0
42	35.70161934283943	38.0	35.0	40.0	27.0	41.0
43	35.63526844389337	38.0	35.0	40.0	27.0	41.0
44	35.449239925604466	38.0	35.0	40.0	26.0	41.0
45	35.321716057036575	38.0	34.0	40.0	26.0	41.0
46	35.18887042777433	38.0	34.0	40.0	26.0	41.0
47	35.073706137631746	38.0	34.0	40.0	25.0	41.0
48	35.000818350898946	38.0	34.0	40.0	25.0	41.0
49	34.835546187228765	38.0	34.0	40.0	24.0	41.0
50	34.60848605083695	37.0	34.0	40.0	24.0	41.0
51	32.579323000619965	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	18.0
10	13.0
11	4.0
12	10.0
13	3.0
14	7.0
15	12.0
16	18.0
17	53.0
18	95.0
19	133.0
20	250.0
21	400.0
22	600.0
23	916.0
24	1361.0
25	1927.0
26	2487.0
27	2759.0
28	3061.0
29	3453.0
30	3994.0
31	5148.0
32	6429.0
33	9040.0
34	13837.0
35	17313.0
36	19115.0
37	30093.0
38	37973.0
39	41085.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87179169249845	19.577929324240547	23.892870427774334	14.65740855548667
2	31.335895846249223	24.07389956602604	26.78784872907626	17.80235585864848
3	32.118040917544946	23.482207067575946	28.030750154990702	16.369001859888407
4	28.4101673899566	25.844513329200247	27.369621822690636	18.37569745815251
5	24.855548667079972	30.462492250464972	26.54135151890887	18.140607563546187
6	26.323620582765034	35.29373837569746	24.831246125232486	13.551394916305021
7	61.92783632982021	27.85269683818971	6.715437073775574	3.5040297582145072
8	84.14035957842529	4.568381897086175	8.03620582765034	3.25505269683819
9	77.5826410415375	7.470303781773094	10.033973961562307	4.913081215127092
10	41.748791072535646	26.70898946063236	17.502293862368255	14.039925604463733
11	32.00099194048357	26.232362058276504	24.460260384376937	17.306385616862986
12	29.464104153750775	22.98425294482331	28.156230626162433	19.395412275263485
13	24.74296342219467	25.181401115933046	29.42492250464972	20.650712957222567
14	21.1863608183509	27.600743955362677	29.18090514569126	22.031990080595165
15	20.641785492870426	25.780037197768134	33.60743955362678	19.970737755734653
16	23.892870427774334	24.429014259144452	32.08133911965282	19.596776193428393
17	23.218350898946063	24.90911345319281	28.325852448853066	23.546683199008058
18	24.659640421574704	25.67588344699318	28.82083075015499	20.843645381277124
19	25.930316181029138	26.941599504029757	26.26807191568506	20.860012399256046
20	27.70142591444513	25.688282703037817	26.577557346559207	20.03273403595784
21	25.389212647241166	27.115189088654677	27.470303781773094	20.02529448233106
22	24.69882207067576	24.775201487910724	26.804711717296964	23.72126472411655
23	23.991568505889646	26.657408555486672	27.507005579665222	21.84401735895846
24	23.52238065716057	24.77371357718537	29.867823930564164	21.836081835089892
25	23.440545567265964	26.056292622442655	27.74804711717297	22.755114693118415
26	22.50663360198388	27.951394916305023	27.652324860508372	21.889646621202726
27	22.935151890886544	27.02393056416615	27.7093614383137	22.331556106633602
28	21.409051456912582	27.723744575325483	29.38276503409795	21.48443893366398
29	22.743707377557346	26.650464972101673	28.437445753254803	22.168381897086174
30	22.830502169869806	26.471419714817113	28.243025418474893	22.45505269683819
31	24.13837569745815	26.31320520768754	26.224426534407936	23.323992560446374
32	24.40024798512089	27.563546187228766	26.30874147551147	21.727464352138874
33	24.533168009919404	27.88791072535648	25.809299442033478	21.769621822690638
34	22.5230006199628	27.21983880967142	27.364662120272783	22.892498450092994
35	22.50663360198388	26.70402975821451	27.945443273403598	22.843893366398017
36	22.30328580285183	29.47303161810291	26.136639801611906	22.087042777433354
37	22.33254804711717	29.573217606943587	26.356850588964665	21.73738375697458
38	22.288406695598265	28.82827030378177	26.672287662740235	22.21103533787973
39	22.799752014879108	27.7163050216987	26.458028518288902	23.025914445133292
40	22.6529448233106	26.335523868567883	28.507873527588345	22.503657780533167
41	20.601115933044017	27.166769993800372	27.938995660260385	24.293118412895225
42	22.29237445753255	27.528828270303784	26.893490390576563	23.285306881587108
43	22.523992560446374	27.319032858028518	26.527960322380657	23.62901425914445
44	21.768133911965283	27.788220706757592	26.619714817110975	23.82393056416615
45	21.89807811531308	26.906881587104774	26.591940483570987	24.60309981401116
46	22.225418474891505	28.499938003719777	25.761686298822074	23.512957222566648
47	22.10737755734656	27.3264724116553	27.366646001239925	23.199504029758213
48	21.849473031618103	26.392064476131434	27.685554866707996	24.072907625542467
49	21.562802231866087	27.215871047737135	27.733168009919407	23.48815871047737
50	19.930068195908245	27.0154990700558	27.8735275883447	25.180905145691256
51	19.927092374457533	27.082455052696837	27.338871667699937	25.651580905145693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	223.5
2	349.0
3	1329.0
4	2309.0
5	1642.5
6	976.0
7	832.5
8	689.0
9	677.0
10	665.0
11	641.5
12	618.0
13	637.0
14	656.0
15	645.5
16	635.0
17	600.0
18	565.0
19	578.5
20	592.0
21	612.5
22	633.0
23	838.0
24	1043.0
25	1192.5
26	1723.5
27	2105.0
28	2467.5
29	2830.0
30	3177.5
31	3525.0
32	4020.0
33	4515.0
34	5215.5
35	5916.0
36	6276.5
37	6637.0
38	7070.0
39	7503.0
40	8471.0
41	9439.0
42	10371.5
43	11304.0
44	12321.0
45	13338.0
46	14232.0
47	15126.0
48	15426.0
49	15726.0
50	15201.0
51	14676.0
52	13547.0
53	12418.0
54	11823.0
55	11228.0
56	10722.0
57	10216.0
58	10138.5
59	10061.0
60	10051.5
61	10042.0
62	8838.5
63	7635.0
64	6386.5
65	5138.0
66	4406.5
67	3675.0
68	3082.0
69	2489.0
70	2015.0
71	1541.0
72	1347.0
73	1153.0
74	1042.0
75	718.5
76	506.0
77	426.0
78	346.0
79	264.0
80	182.0
81	161.0
82	140.0
83	97.5
84	55.0
85	40.0
86	25.0
87	21.5
88	18.0
89	14.0
90	10.0
91	7.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	201625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.76825790452573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64853705417526	34.45406075635462
2	10.239095282164977	9.986856788592686
3	5.3534562540044135	7.832362058276503
4	3.642872397766681	7.106261624302541
5	2.4357005562957013	5.939243645381278
6	1.8631329516215969	5.451704897706137
7	1.456335363931292	4.97160570365778
8	1.1247953299636932	4.388344699318041
9	0.8858017471956392	3.887910725356478
>10	2.332984165403899	14.728332300061997
>50	0.01220392763070915	0.4141351518908865
>100	0.00406797587690305	0.2792312461252325
>500	0.0	0.0
>1k	0.0010169939692257625	0.5599504029758215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1129	0.5599504029758215	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05802851828890267	0.0
2	0.0	0.0	0.0	0.24748915065096094	0.0
3	0.0	0.0	0.0	0.3660260384376937	0.0
4	0.0	0.0	0.0	0.49001859888406696	0.0
5	0.0	0.0	0.0	0.8867947923124613	0.0
6	0.0	0.0	0.0	1.244885306881587	0.0
7	0.0	0.0	0.0	1.5176689398636083	0.0
8	0.0	0.0	0.0	2.1961562306261624	0.0
9	0.0	0.0	0.0	2.4991940483570985	0.0
10	0.0	0.0	0.0	3.1047737135771856	0.0
11	0.0	0.0	0.0	3.7902045877247366	0.0
12	0.0	0.0	0.0	4.290638561686299	0.0
13	0.0	0.0	0.0	4.530192188468692	0.0
14	0.0	0.0	0.0	4.630378177309361	0.0
15	0.0	0.0	0.0	4.807935523868568	0.0
16	0.0	0.0	0.0	5.159578425294482	0.0
17	0.0	0.0	0.0	5.582641041537507	0.0
18	0.0	0.0	0.0	6.066212027278364	0.0
19	0.0	0.0	0.0	6.3528828270303785	0.0
20	0.0	0.0	0.0	6.651952882827031	0.0
21	0.0	0.0	0.0	7.05914445133292	0.0
22	0.0	0.0	0.0	7.44649721016739	0.0
23	0.0	0.0	0.0	7.878487290762554	0.0
24	0.0	0.0	0.0	8.223186608803472	0.0
25	0.0	0.0	0.0	8.541103533787973	0.0
26	0.0	0.0	0.0	8.841661500309982	0.0
27	0.0	0.0	0.0	9.120892746435214	0.0
28	0.0	0.0	0.0	9.45368877867328	0.0
29	0.0	0.0	0.0	9.769125852448854	0.0
30	0.0	0.0	0.0	10.147055176689399	0.0
31	0.0	0.0	0.0	10.463484190948543	0.0
32	0.0	0.0	0.0	10.805207687538747	0.0
33	0.0	0.0	0.0	11.148915065096094	0.0
34	0.0	0.0	0.0	11.46633601983881	0.0
35	0.0	0.0	0.0	11.82492250464972	0.0
36	0.0	0.0	0.0	12.18152510849349	0.0
37	0.0	0.0	0.0	12.52275263484191	0.0
38	0.0	0.0	0.0	12.867451952882828	0.0
39	0.0	0.0	0.0	13.243397396156231	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGGT	25	3.87933E-5	45.000004	10
ACAACGA	20	7.019437E-4	45.0	13
CATGGGT	40	6.7666406E-9	45.0	4
TTTCGCA	20	7.019437E-4	45.0	14
ACGTAGG	20	7.019437E-4	45.0	2
CGACTGG	20	7.019437E-4	45.0	2
CACAACG	20	7.019437E-4	45.0	12
CGTTTTT	400	0.0	42.750004	1
TACGGGA	70	0.0	41.785713	4
TATGGGC	55	6.002665E-11	40.909092	4
CGTTTTA	95	0.0	40.263157	1
TACGGGT	35	6.2226954E-6	38.571426	4
GGCGATA	70	0.0	38.571426	8
ACGGGAT	65	9.094947E-12	38.07692	5
ACGGGAG	65	9.094947E-12	38.07692	5
TTAGCGG	30	1.136586E-4	37.499996	2
TAACGGG	30	1.136586E-4	37.499996	3
CGCCCAA	85	0.0	37.058823	37
ATTTCCG	85	0.0	37.058823	32
TTGCGGG	110	0.0	36.81818	3
>>END_MODULE