FastQCFastQC Report
Sat 18 Jun 2016
SRR3551406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101996
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC1610.1578493274246049TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC1570.1539276050041178TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.13726028471704774No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA1130.11078865837875994No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT1080.1058865053531511Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATAC351.1974225E-745.00000416
GTGTAGG351.1974225E-745.0000042
TGGACGG207.003046E-445.02
GGCACCG207.003046E-445.08
GGCCGAT253.8657607E-545.08
CACGAGG207.003046E-445.02
GACCCAC207.003046E-445.012
AAGGGCG406.710252E-945.05
TAGGACC207.003046E-445.06
AGGGCGG207.003046E-445.06
AGGCGAT302.1460255E-644.9999967
CATACGA302.1460255E-644.99999618
GCGATGG302.1460255E-644.9999969
ATACGAA302.1460255E-644.99999619
CTCGTAA650.041.5384633
CGAATAG650.041.5384626
GCTCGTA650.041.5384632
TAGCTCG650.041.5384630
GTAACCA650.041.5384636
ACGAATA650.041.5384625
Produced by FastQC (version 0.11.5)