FastQCFastQC Report
Sat 18 Jun 2016
SRR3551405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189188
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.3467450366830877No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG4500.2378586379685815No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC2990.15804385056134637No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC2750.14535805653635536No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2390.12632936549886883No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC2010.10624352495929973Illumina PCR Primer Index 3 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC1940.1025435017020107RNA PCR Primer, Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC207.018332E-445.00000431
GGATGCG207.018332E-445.0000048
AAGACGG207.018332E-445.0000042
TATGCGG207.018332E-445.0000042
CCACTGG207.018332E-445.0000042
TCGGACG207.018332E-445.0000041
CGCTCGT207.018332E-445.00000442
GACGAGG207.018332E-445.0000042
TATACTG351.2047349E-745.00000418
TAGTCCT207.018332E-445.00000427
ATCTACC453.8198777E-1045.015
TTTACGG253.8784157E-545.02
TTACTAT253.8784157E-545.015
AATGGGT502.1827873E-1145.04
CAAGGCG253.8784157E-545.05
TCAACGG253.8784157E-545.02
AGACCTA302.1558608E-644.99999632
GACCTAT302.1558608E-644.99999633
CTACCTT603.6379788E-1241.24999617
AATATAC556.002665E-1140.90909216