##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219919
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.072135649943846	33.0	31.0	34.0	30.0	34.0
2	32.223659620132864	33.0	31.0	34.0	30.0	34.0
3	32.1159517822471	33.0	31.0	34.0	30.0	34.0
4	35.84577048822521	37.0	35.0	37.0	35.0	37.0
5	35.89655736884944	37.0	35.0	37.0	35.0	37.0
6	35.868183285664266	37.0	35.0	37.0	35.0	37.0
7	26.68526593882293	35.0	10.0	37.0	0.0	37.0
8	31.100305112336816	36.0	19.0	37.0	17.0	37.0
9	35.97434055265803	39.0	32.0	39.0	32.0	39.0
10	36.69532418754178	38.0	35.0	39.0	33.0	39.0
11	37.06514671310801	39.0	37.0	39.0	34.0	39.0
12	37.135327097704156	39.0	37.0	39.0	34.0	39.0
13	37.15263801672434	39.0	37.0	39.0	34.0	39.0
14	38.32983052851277	40.0	38.0	41.0	34.0	41.0
15	38.419627226387895	40.0	38.0	41.0	34.0	41.0
16	38.344399528917464	40.0	38.0	41.0	34.0	41.0
17	38.263606145899175	40.0	37.0	41.0	34.0	41.0
18	38.17456427139083	40.0	37.0	41.0	34.0	41.0
19	38.051164292307625	40.0	37.0	41.0	34.0	41.0
20	38.09707665094876	40.0	37.0	41.0	34.0	41.0
21	38.02364052219226	40.0	37.0	41.0	33.0	41.0
22	38.037777545368975	40.0	37.0	41.0	33.0	41.0
23	37.96477339384046	40.0	37.0	41.0	33.0	41.0
24	37.914013795988524	40.0	36.0	41.0	33.0	41.0
25	37.77924599511638	40.0	36.0	41.0	33.0	41.0
26	37.6498665417722	40.0	36.0	41.0	33.0	41.0
27	37.51210218307649	40.0	36.0	41.0	33.0	41.0
28	37.49023958821202	40.0	36.0	41.0	33.0	41.0
29	37.47857620305658	40.0	36.0	41.0	33.0	41.0
30	37.371273059626496	40.0	36.0	41.0	32.0	41.0
31	37.27506945739113	40.0	36.0	41.0	32.0	41.0
32	37.12900658878951	39.0	35.0	41.0	32.0	41.0
33	37.015310182385335	39.0	35.0	41.0	31.0	41.0
34	36.88033776072099	39.0	35.0	41.0	31.0	41.0
35	36.783161072940494	39.0	35.0	41.0	31.0	41.0
36	36.62559396868847	39.0	35.0	41.0	30.0	41.0
37	36.57062372964592	39.0	35.0	41.0	30.0	41.0
38	36.4353966687735	39.0	35.0	41.0	30.0	41.0
39	36.388365716468336	39.0	35.0	41.0	30.0	41.0
40	36.21008644091688	39.0	35.0	40.0	30.0	41.0
41	36.12826540680887	39.0	35.0	40.0	29.0	41.0
42	36.05143257290184	39.0	35.0	40.0	29.0	41.0
43	36.01767923644615	39.0	35.0	40.0	29.0	41.0
44	35.82849594623475	38.0	35.0	40.0	28.0	41.0
45	35.6785998481259	38.0	35.0	40.0	28.0	41.0
46	35.57846752668028	38.0	34.0	40.0	28.0	41.0
47	35.456736343835686	38.0	34.0	40.0	27.0	41.0
48	35.39859220894966	38.0	34.0	40.0	27.0	41.0
49	35.23295849835621	38.0	34.0	40.0	27.0	41.0
50	35.055238519636774	38.0	34.0	40.0	26.0	41.0
51	32.96870666017943	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	22.0
10	24.0
11	13.0
12	9.0
13	6.0
14	20.0
15	10.0
16	30.0
17	66.0
18	70.0
19	183.0
20	286.0
21	436.0
22	625.0
23	902.0
24	1256.0
25	1539.0
26	2043.0
27	2387.0
28	2824.0
29	3308.0
30	4133.0
31	5142.0
32	6835.0
33	9636.0
34	15075.0
35	18725.0
36	21317.0
37	34213.0
38	42489.0
39	46271.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.4440680432341	21.465175814731786	25.782674530167927	13.308081611866188
2	31.59026732569719	24.754568727576974	25.99411601544205	17.66104793128379
3	32.09317976163951	24.408077519450345	26.39017092656842	17.108571792341724
4	29.607719205707557	25.32705223286756	26.210104629431747	18.85512393199314
5	25.525761757738074	29.942842592045256	24.893256153401936	19.638139496814734
6	26.51885466921912	34.96741982275292	24.291671024331684	14.22205448369627
7	61.96554185859339	28.67601253188674	5.568413825090147	3.790031784429722
8	85.06450102083039	4.704004656259805	6.769765231744415	3.4617290911653837
9	79.23235373023704	7.284500202347227	8.589071430844992	4.894074636570737
10	42.80439616404222	26.135531718496356	16.275537811648835	14.784534305812594
11	31.607091701944807	27.05268758042734	23.099413875108564	18.24080684251929
12	28.894274710234225	24.196181321304664	27.42191443213183	19.487629536329283
13	24.969193202951995	24.75184045034763	29.22894338370036	21.050022963000014
14	21.090947121440166	26.805323778300195	30.039696433686945	22.064032666572693
15	20.77901409155189	25.99411601544205	31.983139246722658	21.243730646283403
16	23.701908429921925	25.020575757437967	30.679477443968008	20.598038368672103
17	24.068407004397073	24.272118370854724	28.038959798835027	23.620514825913176
18	25.31113728236305	25.738112668755313	27.648361442167342	21.30238860671429
19	26.77804100600676	26.52703950090715	25.67945470832443	21.01546478476166
20	27.98666781860594	25.429817341839495	26.3042301938441	20.279284645710465
21	25.985021758010905	26.28058512452312	27.21320122408705	20.521191893378926
22	25.310682569491494	24.6340698166143	27.034953778436606	23.0202938354576
23	24.59723807401816	26.346518490898923	27.115437956702245	21.940805478380675
24	24.20163787576335	25.012845638621496	28.312242234640937	22.473274250974224
25	23.99428880633324	25.92409023322223	27.271404471646377	22.810216488798147
26	23.41407518222618	27.259127224114334	26.283313401752462	23.04348419190702
27	22.741100132321446	27.283681719178425	27.296868392453582	22.678349756046543
28	21.46835880483269	28.197199878136953	28.05442003646797	22.28002128056239
29	23.198086568236487	27.232753877564015	27.446923640067478	22.12223591413202
30	23.470004865427725	26.24284395618387	27.704291125368886	22.58286005301952
31	24.32077264811135	26.828059421878052	25.488020589398825	23.36314734061177
32	24.58905324233013	27.295504253838914	25.698552648929834	22.41688985490112
33	24.365789222395517	27.35779991724226	25.762212450947846	22.514198409414377
34	22.70199482536752	27.345977382581772	27.867078333386385	22.084949458664326
35	23.003014746338422	27.04086504576685	27.1381736002801	22.817946607614623
36	23.45090692482232	29.223486829241672	25.49666013395841	21.828946111977594
37	23.097595023622333	29.068429740040653	25.80495546087423	22.029019775462785
38	22.988463934448593	28.398637680236817	25.798134767800875	22.81476361751372
39	23.879246449829257	26.671183481190802	26.1446259759275	23.304944093052445
40	23.748289142820767	26.138714708597256	27.823880610588443	22.289115537993535
41	21.23645524033849	26.91672843183172	28.0498729077524	23.796943420077394
42	22.81203534028438	27.42055029351716	26.079602035294812	23.68781233090365
43	23.299487538593755	26.302411342357868	26.254666490844357	24.143434628204023
44	23.02711452853096	26.32560169880729	26.81578217434601	23.831501598315743
45	23.155798271181663	26.344244926541137	26.41336128301784	24.08659551925936
46	22.489189201478727	27.330062432077263	26.55568641181526	23.62506195462875
47	22.387333518249903	27.482845956920503	27.092702313124377	23.037118211705216
48	22.343226369708844	26.100064114514886	28.02804668991765	23.52866282585861
49	22.101773834911945	26.768492035704057	27.299141956811372	23.83059217257263
50	20.851768151001053	26.768492035704057	28.33634201683347	24.043397796461424
51	21.37832565626435	25.9095394213324	27.099523006197735	25.612611916205513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	251.0
2	440.0
3	742.0
4	1044.0
5	741.0
6	438.0
7	434.5
8	431.0
9	436.0
10	441.0
11	451.0
12	461.0
13	478.0
14	495.0
15	499.0
16	503.0
17	515.0
18	527.0
19	596.0
20	665.0
21	792.5
22	920.0
23	1109.5
24	1299.0
25	1422.0
26	1891.0
27	2237.0
28	2762.0
29	3287.0
30	3888.0
31	4489.0
32	4987.0
33	5485.0
34	6055.5
35	6626.0
36	7045.0
37	7464.0
38	8033.5
39	8603.0
40	9467.0
41	10331.0
42	11100.5
43	11870.0
44	12629.5
45	13389.0
46	14451.0
47	15513.0
48	16100.5
49	16688.0
50	16343.0
51	15998.0
52	15079.0
53	14160.0
54	13245.5
55	12331.0
56	12065.5
57	11800.0
58	11584.5
59	11369.0
60	10977.5
61	10586.0
62	9646.5
63	8707.0
64	7706.5
65	6706.0
66	5640.0
67	4574.0
68	3828.5
69	3083.0
70	2526.0
71	1969.0
72	1707.0
73	1445.0
74	1144.5
75	677.0
76	510.0
77	381.0
78	252.0
79	199.0
80	146.0
81	116.5
82	87.0
83	64.5
84	42.0
85	34.0
86	26.0
87	18.0
88	10.0
89	10.5
90	11.0
91	8.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	219919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.555803581597274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.136	30.128786256725178
2	11.111	10.123410671902548
3	6.375	8.712547434980479
4	4.444	8.097999644664732
5	3.024	6.888037501537508
6	2.335	6.382368081781778
7	1.6660000000000001	5.312717813685874
8	1.355	4.938249108245145
9	1.016	4.165622679501255
>10	2.521	14.454856476440817
>50	0.013	0.4300467858102783
>100	0.004	0.3653575447244102
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	396	0.18006629713667305	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027737485164992564	0.0
2	0.0	0.0	0.0	0.17915687139355854	0.0
3	0.0	0.0	0.0	0.2628240397600935	0.0
4	0.0	0.0	0.0	0.39059835666768217	0.0
5	0.0	0.0	0.0	0.7620987727299596	0.0
6	0.0	0.0	0.0	1.037200060022099	0.0
7	0.0	0.0	0.0	1.281380872048345	0.0
8	0.0	0.0	0.0	1.7479162782660889	0.0
9	0.0	0.0	0.0	1.9616313278979989	0.0
10	0.0	0.0	0.0	2.4404439816477885	0.0
11	0.0	0.0	0.0	3.0747684374701594	0.0
12	0.0	0.0	0.0	3.508109804064224	0.0
13	0.0	0.0	0.0	3.713185309136546	0.0
14	0.0	0.0	0.0	3.7909412101728366	0.0
15	0.0	0.0	0.0	3.936449329071158	0.0
16	0.0	0.0	0.0	4.275210418381313	0.0
17	0.0	0.0	0.0	4.655805091874735	0.0
18	0.0	0.0	0.0	5.065501389147823	0.0
19	0.0	0.0	0.0	5.322414161577671	0.0
20	0.0	0.0	0.0	5.582509924108422	0.0
21	4.5471287155725515E-4	0.0	0.0	5.895352379739813	0.0
22	4.5471287155725515E-4	0.0	0.0	6.233204043306854	0.0
23	4.5471287155725515E-4	0.0	0.0	6.557414320727177	0.0
24	4.5471287155725515E-4	0.0	0.0	6.802504558496538	0.0
25	4.5471287155725515E-4	0.0	0.0	7.050323073495242	0.0
26	4.5471287155725515E-4	0.0	0.0	7.265402261741823	0.0
27	4.5471287155725515E-4	0.0	0.0	7.491849271777336	0.0
28	4.5471287155725515E-4	0.0	0.0	7.740577212519155	0.0
29	4.5471287155725515E-4	0.0	0.0	7.972935489884912	0.0
30	4.5471287155725515E-4	0.0	0.0	8.274410123727373	0.0
31	4.5471287155725515E-4	0.0	0.0	8.525411628826976	0.0
32	4.5471287155725515E-4	0.0	0.0	8.792782797302642	0.0
33	4.5471287155725515E-4	0.0	0.0	9.06333695587921	0.0
34	4.5471287155725515E-4	0.0	0.0	9.328889272868647	0.0
35	4.5471287155725515E-4	0.0	0.0	9.615358381949719	0.0
36	4.5471287155725515E-4	0.0	0.0	9.877727708838254	0.0
37	4.5471287155725515E-4	0.0	0.0	10.128274501066302	0.0
38	4.5471287155725515E-4	0.0	0.0	10.420654877477617	0.0
39	4.5471287155725515E-4	0.0	0.0	10.734406758852122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACC	20	7.020836E-4	45.0	7
CAACGAC	25	3.880488E-5	45.0	12
CGCGCAA	25	3.880488E-5	45.0	18
GCGCAAT	25	3.880488E-5	45.0	19
GTATGCG	20	7.020836E-4	45.0	1
CGCAATC	25	3.880488E-5	45.0	20
ACGACGC	25	3.880488E-5	45.0	14
CGAAGTC	20	7.020836E-4	45.0	26
GCGATTG	60	0.0	44.999996	9
ACGGGGC	60	3.6379788E-12	41.249996	5
TTAGCGG	55	6.002665E-11	40.909092	2
ACGGGAT	40	3.441237E-7	39.375	5
AACACGT	35	6.225273E-6	38.571426	41
CGTGGAA	30	1.1369223E-4	37.499996	25
CGACGCG	30	1.1369223E-4	37.499996	15
CACGACC	30	1.1369223E-4	37.499996	27
CGTTCAT	30	1.1369223E-4	37.499996	17
CATACGA	30	1.1369223E-4	37.499996	18
GCGATGC	30	1.1369223E-4	37.499996	9
GCTAGCG	30	1.1369223E-4	37.499996	1
>>END_MODULE