FastQCFastQC Report
Sat 18 Jun 2016
SRR3551400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254377
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.34948128171965237No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATA207.022918E-445.0000041
TTGACGG207.022918E-445.0000042
TTACGCG207.022918E-445.0000041
AGTACGG351.2069358E-745.0000042
CACAACG551.8189894E-1245.012
ACAACGA603.6379788E-1241.24999613
ACGAGCG603.6379788E-1241.24999616
GCGATGC556.002665E-1140.9090929
AGGCGAT900.040.07
CACGACC403.4440745E-739.37500427
CGTTTTT4100.038.9634131
GCGATTG356.22912E-638.571439
TACGAAT659.094947E-1238.0769212
TGCGACG301.13742426E-437.4999961
CGAATAT601.546141E-1037.49999614
GCGATTC301.13742426E-437.4999969
TGGGCCG301.13742426E-437.4999966
ATGGGCG552.732122E-936.818185
CGATGCG552.732122E-936.8181810
CGATTGG250.002104031636.010