##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132317
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.933084939954806	33.0	31.0	34.0	30.0	34.0
2	32.02232517363604	33.0	31.0	34.0	30.0	34.0
3	31.970608463009288	33.0	31.0	34.0	30.0	34.0
4	35.720753946960706	37.0	35.0	37.0	35.0	37.0
5	35.7727729619021	37.0	35.0	37.0	35.0	37.0
6	35.73688943975453	37.0	35.0	37.0	35.0	37.0
7	26.802406342344522	35.0	11.0	37.0	0.0	37.0
8	31.053077080042623	35.0	19.0	37.0	17.0	37.0
9	35.80750772765404	38.0	32.0	39.0	32.0	39.0
10	36.631604404573864	37.0	35.0	39.0	33.0	39.0
11	36.95632458414263	39.0	37.0	39.0	33.0	39.0
12	36.91680585261153	39.0	35.0	39.0	33.0	39.0
13	36.76430088348436	39.0	35.0	39.0	33.0	39.0
14	37.97905031099556	40.0	37.0	41.0	33.0	41.0
15	38.0344551342609	40.0	37.0	41.0	33.0	41.0
16	38.02197752367421	40.0	36.0	41.0	33.0	41.0
17	37.89668750047235	40.0	36.0	41.0	33.0	41.0
18	37.77275784668637	40.0	36.0	41.0	33.0	41.0
19	37.66516018349872	40.0	36.0	41.0	33.0	41.0
20	37.744280780247436	40.0	36.0	41.0	33.0	41.0
21	37.70458822373542	40.0	36.0	41.0	33.0	41.0
22	37.69449881723437	39.0	36.0	41.0	33.0	41.0
23	37.704353937891575	39.0	36.0	41.0	33.0	41.0
24	37.61083609815821	39.0	36.0	41.0	33.0	41.0
25	37.558552566941515	39.0	35.0	41.0	33.0	41.0
26	37.367874120483386	39.0	35.0	41.0	33.0	41.0
27	37.27819554554593	39.0	35.0	41.0	32.0	41.0
28	37.265634801272704	39.0	35.0	41.0	32.0	41.0
29	37.25361820476583	39.0	35.0	41.0	32.0	41.0
30	37.18096692035037	39.0	35.0	41.0	32.0	41.0
31	37.0675801295374	39.0	35.0	41.0	32.0	41.0
32	36.90976216208046	39.0	35.0	41.0	31.0	41.0
33	36.82397575519396	39.0	35.0	41.0	31.0	41.0
34	36.72339155210593	39.0	35.0	41.0	31.0	41.0
35	36.5415479492431	39.0	35.0	41.0	30.0	41.0
36	36.44109222548879	39.0	35.0	41.0	30.0	41.0
37	36.35748240966769	39.0	35.0	41.0	30.0	41.0
38	36.20343568853586	39.0	35.0	40.0	30.0	41.0
39	36.161755481155105	39.0	35.0	40.0	30.0	41.0
40	35.78605167892259	38.0	35.0	40.0	28.0	41.0
41	35.78268854342224	38.0	35.0	40.0	28.0	41.0
42	35.688649228746115	38.0	35.0	40.0	28.0	41.0
43	35.72099579041242	38.0	35.0	40.0	28.0	41.0
44	35.49058699940295	38.0	34.0	40.0	27.0	41.0
45	35.42179009499913	38.0	34.0	40.0	27.0	41.0
46	35.20902076074881	38.0	34.0	40.0	26.0	41.0
47	35.09068373678363	38.0	34.0	40.0	26.0	41.0
48	34.96750984378424	38.0	34.0	40.0	25.0	41.0
49	34.75902567319392	37.0	34.0	40.0	24.0	41.0
50	34.565354414020874	37.0	34.0	40.0	24.0	41.0
51	32.15815805981091	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	8.0
11	8.0
12	4.0
13	5.0
14	6.0
15	10.0
16	14.0
17	32.0
18	70.0
19	113.0
20	182.0
21	280.0
22	365.0
23	519.0
24	770.0
25	1043.0
26	1342.0
27	1655.0
28	2041.0
29	2366.0
30	2936.0
31	3661.0
32	4783.0
33	6459.0
34	10315.0
35	12672.0
36	12217.0
37	18812.0
38	23941.0
39	25665.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.37978491048013	18.351383420119863	21.943514438809828	15.325317230590175
2	27.31395058835977	29.227536900020407	25.66639207358087	17.792120438038953
3	30.810855747938664	27.351738627689564	26.29745233038839	15.539953293983388
4	28.802043577166952	24.1578935435356	28.508808391967776	18.531254487329672
5	27.353250149262752	28.794485969300997	24.06191192363793	19.79035195779832
6	25.89463183113281	35.95305213993667	24.03243725296069	14.11987877596983
7	58.6568619300619	27.9971583394424	8.673866547760303	4.6721131827354005
8	77.44583084562075	7.909036631725326	9.355562777269737	5.289569745384191
9	71.71187375771821	8.382142884134314	10.050862700937898	9.85512065720958
10	43.968651042572006	24.311312983214552	17.443714715418277	14.276321258795166
11	34.98492257230741	24.075515617796654	22.760491849119916	18.179069960776015
12	32.02082876727858	21.56109948079234	27.303369937347433	19.11470181458165
13	23.45050144728191	26.92624530483611	29.722560215240673	19.90069303264131
14	18.84640673534013	30.723187496693548	27.078153222941875	23.35225254502445
15	18.008268023005357	25.361820476582754	37.1788961357951	19.45101536461679
16	20.339790049653484	24.421654058057545	32.382082423271385	22.856473469017587
17	21.180196044348044	23.721819569669808	29.1738778841721	25.924106501810044
18	21.844509775765776	26.402503079725204	29.607684575678107	22.145302568830914
19	25.048179750145483	27.197563427224015	25.25299092331295	22.50126589931755
20	26.936070195061856	26.20751679678348	27.39103818859255	19.46537481956211
21	24.429211665923503	28.197434947890294	26.472788832878617	20.90056455330759
22	21.74777239508151	26.360180475675836	25.299848092081895	26.592199037160757
23	22.683404248887143	28.795997490874186	26.85218074774972	21.668417512488947
24	24.154114739602623	23.669672075394697	28.6312416393963	23.544971545606387
25	20.76226032936055	29.343924061156162	26.616383382331826	23.27743222715146
26	20.32769787706795	27.995646817869208	26.939093238208244	24.737562066854597
27	22.474814271786695	28.414338293643297	27.024494207093568	22.086353227476437
28	19.01645291232419	26.4765676368116	31.615740985663216	22.891238465200995
29	22.454408730548607	24.65442838032906	27.04036518361208	25.850797705510253
30	20.736564462616293	26.25286244397923	30.326413083730735	22.684160009673736
31	23.798907169902584	25.05573735801144	27.477950679051066	23.66740479303491
32	25.761617932691944	25.801673254381523	25.421525578723823	23.01518323420271
33	22.547367307299893	25.14264984846996	28.273766787336474	24.03621605689367
34	21.41750493133913	24.823718796526524	29.495831979261922	24.262944292872422
35	22.311569941881995	23.248713317260822	28.816403032112277	25.62331370874491
36	21.70393826945895	26.371516887474776	28.463462744772023	23.46108209829425
37	21.607200888774685	26.201470710490714	30.96503094840421	21.22629745233039
38	21.17188267569549	26.939848998994837	27.935185954941545	23.953082370368133
39	23.269118858498906	24.001451060710263	29.053711919103364	23.675718161687463
40	24.21759864567667	22.95169932812866	30.196422228436255	22.634279797758413
41	19.205393108973148	25.724585654148747	28.449859050613302	26.6201621862648
42	22.06745920781154	24.844880098551204	28.448347529040106	24.639313164597144
43	23.551773392685746	24.832032165179076	26.50453078591564	25.111663656219534
44	21.22629745233039	26.15688082408156	27.864900201788128	24.75192152179992
45	21.19379973850677	25.212935601623375	26.50528654670224	27.087978113167622
46	22.688694574393313	27.4900428516366	26.63678892356991	23.184473650400175
47	20.475826991240734	24.20626223387773	32.004957790760066	23.312952984121466
48	22.205007670971984	23.095293877581867	28.97813584044378	25.721562611002362
49	19.404913956634445	22.93431683003696	32.26116069741605	25.39960851591254
50	19.75331967925512	24.586409909535433	30.314320911145202	25.34594950006424
51	20.348859179092635	23.69612370292555	27.13710256429635	28.81791455368547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	121.0
2	218.0
3	354.0
4	490.0
5	389.5
6	289.0
7	247.0
8	205.0
9	227.0
10	249.0
11	218.5
12	188.0
13	208.0
14	228.0
15	236.0
16	244.0
17	262.0
18	280.0
19	303.5
20	327.0
21	341.0
22	355.0
23	417.5
24	480.0
25	721.5
26	1019.0
27	1075.0
28	1426.5
29	1778.0
30	2016.5
31	2255.0
32	2599.5
33	2944.0
34	3203.0
35	3462.0
36	3714.5
37	3967.0
38	4463.5
39	4960.0
40	5783.5
41	6607.0
42	7319.0
43	8031.0
44	9005.5
45	9980.0
46	12757.0
47	15534.0
48	13382.0
49	11230.0
50	10876.5
51	10523.0
52	9366.0
53	8209.0
54	7357.5
55	6506.0
56	6247.5
57	5989.0
58	5892.0
59	5795.0
60	5615.5
61	5436.0
62	4952.0
63	4468.0
64	3833.0
65	3198.0
66	2653.0
67	2108.0
68	1781.0
69	1454.0
70	1217.5
71	981.0
72	758.5
73	536.0
74	432.5
75	271.5
76	214.0
77	152.0
78	90.0
79	76.5
80	63.0
81	43.5
82	24.0
83	20.0
84	16.0
85	10.5
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	132317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.823288012878166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.62824910938117	31.905953127716014
2	10.357566961340545	9.492355479643583
3	5.521836653912126	7.590861340568483
4	3.6828737300435415	6.750455345873924
5	2.643818445705238	6.057422704565551
6	2.012138804591635	5.532168957881451
7	1.490961868320359	4.782454257578391
8	1.2270748119804724	4.49828820181836
9	0.9219554030874785	3.802232517363604
>10	2.462396094471566	14.381372008131986
>50	0.02144082332761578	0.6862307942290106
>100	0.024739411531864364	2.0654942297663945
>500	0.0016492941021242907	0.6816962295094358
>1k	0.0032985882042485815	1.7730148053538095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	1229	0.928830006726271	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	1117	0.8441847986275385	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	902	0.6816962295094358	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	329	0.248645298790027	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	301	0.2274839967653438	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	257	0.19423052215512746	No Hit
GAATCTATCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	247	0.18667291428916918	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	197	0.14888487495937786	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	196	0.14812911417278202	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	184	0.1390599847336321	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	183	0.1383042239470363	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	167	0.12621205136150304	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.152663678892357	0.0
2	0.0	0.0	0.0	0.9719083715622331	0.0
3	0.0	0.0	0.0	1.2001481291141727	0.0
4	0.0	0.0	0.0	1.6037243891563442	0.0
5	0.0	0.0	0.0	3.4251078848522867	0.0
6	0.0	0.0	0.0	3.9707671727744733	0.0
7	0.0	0.0	0.0	4.7212376338641295	0.0
8	0.0	0.0	0.0	5.802731319482757	0.0
9	0.0	0.0	0.0	6.16625225783535	0.0
10	0.0	0.0	0.0	8.123672695118541	0.0
11	0.0	0.0	0.0	9.029829878246938	0.0
12	0.0	0.0	0.0	10.728780126514355	0.0
13	0.0	0.0	0.0	11.111195084531843	0.0
14	0.0	0.0	0.0	11.275950936009734	0.0
15	0.0	0.0	0.0	11.63871611357573	0.0
16	0.0	0.0	0.0	12.143564319021744	0.0
17	0.0	0.0	0.0	12.710384908968614	0.0
18	0.0	0.0	0.0	13.347491252068895	0.0
19	0.0	0.0	0.0	13.701943060982337	0.0
20	0.0	0.0	0.0	14.071510085627697	0.0
21	0.0	0.0	0.0	14.51816471050583	0.0
22	0.0	0.0	0.0	14.976155747182903	0.0
23	0.0	0.0	0.0	15.465132976110402	0.0
24	0.0	0.0	0.0	15.84754793412789	0.0
25	0.0	0.0	0.0	16.189907570455798	0.0
26	0.0	0.0	0.0	16.458958410483913	0.0
27	0.0	0.0	0.0	16.77486641928097	0.0
28	0.0	0.0	0.0	17.080193777065684	0.0
29	0.0	0.0	0.0	17.387788417210185	0.0
30	0.0	0.0	0.0	17.788341634105972	0.0
31	0.0	0.0	0.0	18.15639713717814	0.0
32	0.0	0.0	0.0	18.488931883280305	0.0
33	0.0	0.0	0.0	18.811641739156723	0.0
34	0.0	0.0	0.0	19.121503661661013	0.0
35	0.0	0.0	0.0	19.498628294172327	0.0
36	7.557607865958267E-4	0.0	0.0	19.872729883537264	0.0
37	7.557607865958267E-4	0.0	0.0	20.22264712773113	0.0
38	7.557607865958267E-4	0.0	0.0	20.568029807205424	0.0
39	7.557607865958267E-4	0.0	0.0	20.896029988588012	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCTT	50	2.1827873E-11	45.0	7
TCGCAAG	20	7.010651E-4	45.0	2
GTCTATG	20	7.010651E-4	45.0	16
GTTAGGC	20	7.010651E-4	45.0	17
TGAAACG	20	7.010651E-4	45.0	4
TGTTCGG	20	7.010651E-4	45.0	2
ACCAGTC	20	7.010651E-4	45.0	12
TGTTATC	25	3.8720544E-5	45.0	24
ACTAAGG	20	7.010651E-4	45.0	3
TTACGGG	20	7.010651E-4	45.0	3
GGCGATC	25	3.8720544E-5	45.0	8
TGGGACC	20	7.010651E-4	45.0	6
AGTACGG	20	7.010651E-4	45.0	2
GTTATCT	25	3.8720544E-5	45.0	25
TTGGGCG	20	7.010651E-4	45.0	5
CTATCTC	55	6.002665E-11	40.909092	5
TATCTCT	55	6.002665E-11	40.909092	6
TTATGGG	70	0.0	38.571426	3
GTCTAAT	35	6.2064755E-6	38.571426	19
AACTTAA	255	0.0	37.941177	45
>>END_MODULE