##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551396_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100429
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09673500682074	33.0	31.0	34.0	30.0	34.0
2	32.22005595993189	33.0	31.0	34.0	30.0	34.0
3	32.134781786137474	33.0	31.0	34.0	30.0	34.0
4	35.873423015264514	37.0	35.0	37.0	35.0	37.0
5	35.93006004241803	37.0	35.0	37.0	35.0	37.0
6	35.91453663782374	37.0	35.0	37.0	35.0	37.0
7	27.12232522478567	36.0	18.0	37.0	0.0	37.0
8	31.328719792091924	37.0	25.0	37.0	17.0	37.0
9	36.05517330651505	39.0	32.0	39.0	32.0	39.0
10	36.82926246402931	38.0	35.0	39.0	34.0	39.0
11	37.1701600135419	39.0	37.0	39.0	34.0	39.0
12	37.13180455844427	39.0	37.0	39.0	34.0	39.0
13	36.95208555297773	39.0	37.0	39.0	33.0	39.0
14	38.14392257216541	40.0	37.0	41.0	33.0	41.0
15	38.24603451194376	40.0	37.0	41.0	33.0	41.0
16	38.23103884336198	40.0	37.0	41.0	34.0	41.0
17	38.149767497436	40.0	37.0	41.0	34.0	41.0
18	38.06328849236774	40.0	36.0	41.0	34.0	41.0
19	37.92200460026486	40.0	36.0	41.0	34.0	41.0
20	38.000438120463215	40.0	36.0	41.0	34.0	41.0
21	37.92176562546675	40.0	36.0	41.0	34.0	41.0
22	37.933445518724675	40.0	36.0	41.0	34.0	41.0
23	37.89078851726095	40.0	36.0	41.0	34.0	41.0
24	37.8779237072957	40.0	36.0	41.0	34.0	41.0
25	37.76188152824383	40.0	36.0	41.0	33.0	41.0
26	37.622579135508666	39.0	35.0	41.0	33.0	41.0
27	37.49673898973404	39.0	35.0	41.0	33.0	41.0
28	37.510251023110854	39.0	36.0	41.0	33.0	41.0
29	37.48874329128041	40.0	36.0	41.0	33.0	41.0
30	37.42663971561999	39.0	36.0	41.0	33.0	41.0
31	37.28005854882554	39.0	35.0	41.0	33.0	41.0
32	37.07480906909359	39.0	35.0	41.0	32.0	41.0
33	37.01334275956148	39.0	35.0	41.0	32.0	41.0
34	36.84379014029812	39.0	35.0	41.0	31.0	41.0
35	36.62901154049129	39.0	35.0	41.0	30.0	41.0
36	36.4124605442651	39.0	35.0	41.0	30.0	41.0
37	36.35010803652332	39.0	35.0	41.0	30.0	41.0
38	36.0437423453385	39.0	35.0	41.0	29.0	41.0
39	36.02145794541417	39.0	35.0	41.0	29.0	41.0
40	35.6260343127981	38.0	35.0	40.0	27.0	41.0
41	35.4351034063866	38.0	35.0	40.0	26.0	41.0
42	35.43288293222077	38.0	35.0	40.0	26.0	41.0
43	35.33582929233588	38.0	35.0	40.0	26.0	41.0
44	35.066564438558586	38.0	34.0	40.0	24.0	41.0
45	34.95092055083691	38.0	34.0	40.0	24.0	41.0
46	34.70781348017007	38.0	34.0	40.0	23.0	41.0
47	34.59493771719324	38.0	34.0	40.0	23.0	41.0
48	34.4652640173655	37.0	34.0	40.0	23.0	41.0
49	34.25196905276364	37.0	34.0	40.0	22.0	41.0
50	34.08184886835476	37.0	33.0	40.0	21.0	41.0
51	31.741957004450907	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	8.0
11	4.0
12	2.0
13	2.0
14	2.0
15	10.0
16	11.0
17	23.0
18	49.0
19	64.0
20	104.0
21	184.0
22	237.0
23	433.0
24	635.0
25	957.0
26	1153.0
27	1345.0
28	1493.0
29	1821.0
30	2185.0
31	2594.0
32	3320.0
33	4619.0
34	7429.0
35	9770.0
36	8843.0
37	14239.0
38	18687.0
39	20186.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03608519451553	18.325384102201557	22.703601549353277	14.934929153929641
2	29.635862151370617	26.723356799330872	25.897898017504907	17.742883031793603
3	31.792609704368257	24.79164384789254	27.055930060042417	16.359816387696778
4	29.29731452070617	25.802308098258468	26.7273397126328	18.173037668402554
5	25.46376046759402	30.282089834609526	24.344561829750372	19.90958786804608
6	27.363610112616875	35.00980792400601	23.26519232492607	14.361389638451044
7	60.97441973931832	27.69717910165391	6.980055561640562	4.348345597387209
8	80.22184827091776	7.151320833623755	8.03353613000229	4.593294765456193
9	74.58303876370371	8.289438309651596	9.838791584104193	7.288731342540501
10	42.79142478766094	27.13957123938305	16.540043214609327	13.528960758346692
11	36.51236196716088	25.082396518933773	22.728494757490367	15.67674675641498
12	34.62346533371835	22.204741658285954	25.64697447948302	17.524818528512682
13	25.289508010634375	28.202013362674126	27.692200460026484	18.816278166665008
14	18.24074719453544	32.81721415129096	26.519232492606715	22.42280616156688
15	18.053550269344512	26.101026595903576	36.495434585627656	19.349988549124255
16	21.272739945633234	23.92137729141981	34.97794461759053	19.827938145356423
17	20.018122255523803	24.23602744227265	29.08522438737815	26.6606259148254
18	23.31995738282767	25.83516713299943	28.35137261149668	22.493502872676217
19	26.402732278525125	26.663613099801854	26.006432404982625	20.927222216690396
20	29.6249091397903	24.428203009091	26.26333031295741	19.68355753816129
21	25.404016768065002	27.377550309173643	26.320086827509982	20.898346095251373
22	22.972448197233867	25.442850172758863	25.627059912973344	25.957641717033926
23	22.657798046381025	28.271714345457987	26.39377072359577	22.676716884565217
24	24.164335002837824	23.79193260910693	29.612960399884496	22.430771988170747
25	20.963068436407813	27.41339652889106	28.568441386452122	23.055093648249013
26	21.264774119029365	29.148951000209106	27.58366607254877	22.002608808212766
27	22.735464855768754	29.64382797797449	27.203297852214003	20.417409314042757
28	19.66264724332613	29.19973314480877	29.771281203636402	21.3663384082287
29	21.941869380358263	26.566031723904448	28.582381583008893	22.909717312728397
30	23.757082117715004	25.992492208425855	28.516663513526968	21.733762160332176
31	25.269593444124705	26.89661352796503	24.98680659968734	22.846986428222923
32	25.915821127363607	27.80770494578259	26.167740393710982	20.108733533142818
33	24.34655328640134	27.28494757490366	26.163757480409046	22.204741658285954
34	22.777285445439066	27.349669916060105	27.76588435611228	22.107160282388552
35	23.757082117715004	24.673152177159984	28.10841490007866	23.46135080504635
36	23.686385406605662	27.37356739587171	27.477123141722014	21.462924055800613
37	24.46902787043583	27.724063766441965	27.775841639367115	20.03106672375509
38	22.163916796941123	27.034024036881775	27.411405072240093	23.39065409393701
39	24.545698951498075	24.44513039062422	28.318513576755716	22.690657081121987
40	25.000746796244112	24.952951836620898	29.596033018351275	20.450268348783716
41	20.1157036314212	26.193629330173557	29.45961823776001	24.231048800645233
42	22.397912953429785	26.215535353334197	28.363321351402483	23.023230341833532
43	25.622081271345927	24.617391390932898	27.197323482261098	22.563203855460078
44	22.937597705841938	27.662328610261977	25.977556283543596	23.42251740035249
45	21.567475529976402	27.0629001583208	25.95166734708102	25.417956964621773
46	22.24556651963078	27.944119726373856	26.83388264345956	22.976431110535803
47	22.725507572513916	25.724641288870743	29.872845492835737	21.677005645779605
48	24.161347817861376	23.26021368329865	28.915950572045922	23.662487926794054
49	22.288382837626582	23.572872377500524	30.37170538390306	23.76703940096984
50	20.374592996046957	26.69647213454281	28.759621224944986	24.169313644465245
51	21.149269633273256	25.976560555218114	26.62278823845702	26.251381573051606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	99.0
2	169.0
3	270.0
4	371.0
5	281.5
6	192.0
7	180.0
8	168.0
9	165.0
10	162.0
11	180.0
12	198.0
13	191.0
14	184.0
15	201.0
16	218.0
17	234.5
18	251.0
19	258.5
20	266.0
21	284.0
22	302.0
23	342.5
24	383.0
25	505.5
26	847.5
27	1067.0
28	1191.0
29	1315.0
30	1575.0
31	1835.0
32	2027.0
33	2219.0
34	2467.0
35	2715.0
36	2894.0
37	3073.0
38	3433.5
39	3794.0
40	4348.5
41	4903.0
42	5703.0
43	6503.0
44	7090.5
45	7678.0
46	8492.0
47	9306.0
48	8916.0
49	8526.0
50	8251.5
51	7977.0
52	7432.0
53	6887.0
54	6368.5
55	5850.0
56	5311.5
57	4773.0
58	4593.0
59	4413.0
60	4501.5
61	4590.0
62	4137.5
63	3685.0
64	2940.5
65	2196.0
66	1746.5
67	1297.0
68	1105.0
69	913.0
70	719.0
71	525.0
72	439.0
73	353.0
74	288.0
75	180.5
76	138.0
77	102.0
78	66.0
79	47.0
80	28.0
81	27.0
82	26.0
83	22.0
84	18.0
85	14.5
86	11.0
87	7.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	100429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.801222754383694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.48853240287647	32.52148283862231
2	9.880430619973618	9.248324687092374
3	5.6401855240202545	7.9190273725716676
4	3.765797200119144	7.04975654442442
5	2.6786094208757074	6.268109808919735
6	2.099910642100336	5.896703143514324
7	1.5999319177907323	5.241513905346065
8	1.1914386621845878	4.460862898166864
9	1.0063401557380538	4.2388154815840045
>10	2.619037487766478	15.678738213065946
>50	0.01489298327730735	0.4261717233070129
>100	0.01489298327730735	1.050493383385277
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	254	0.25291499467285344	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	170	0.16927381533222474	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144	0.1433848788696492	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	137	0.1364147805912635	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	128	0.12745322566191042	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	115	0.11450875743062262	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	107	0.10654293082675323	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15832080375190433	0.0
2	0.0	0.0	0.0	0.7667108106224297	0.0
3	0.0	0.0	0.0	0.9489290941859423	0.0
4	0.0	0.0	0.0	1.2098099154626651	0.0
5	0.0	0.0	0.0	2.209521154248275	0.0
6	0.0	0.0	0.0	2.643658704159157	0.0
7	0.0	0.0	0.0	3.101693733881648	0.0
8	0.0	0.0	0.0	4.006810781746308	0.0
9	0.0	0.0	0.0	4.300550637763992	0.0
10	0.0	0.0	0.0	5.470531420207311	0.0
11	0.0	0.0	0.0	6.288024375429408	0.0
12	0.0	0.0	0.0	7.216043174780193	0.0
13	0.0	0.0	0.0	7.51874458572723	0.0
14	0.0	0.0	0.0	7.63524479980882	0.0
15	0.0	0.0	0.0	7.92500174252457	0.0
16	0.0	0.0	0.0	8.400959882105766	0.0
17	0.0	0.0	0.0	8.97947803921178	0.0
18	0.0	0.0	0.0	9.607782612591981	0.0
19	0.0	0.0	0.0	9.957283254836751	0.0
20	0.0	0.0	0.0	10.31474972368539	0.0
21	0.0	0.0	0.0	10.768801840105946	0.0
22	0.0	0.0	0.0	11.24675143633811	0.0
23	0.0	0.0	0.0	11.70179928108415	0.0
24	0.0	0.0	0.0	12.048312738352468	0.0
25	0.0	0.0	0.0	12.352009877624988	0.0
26	0.0	0.0	0.0	12.65271983192106	0.0
27	0.0	0.0	0.0	12.97832299435422	0.0
28	0.0	0.0	0.0	13.317866353344154	0.0
29	0.0	0.0	0.0	13.635503689173445	0.0
30	0.0	0.0	0.0	14.038773660994334	0.0
31	0.0	0.0	0.0	14.378317019984268	0.0
32	0.0	0.0	0.0	14.716864650648716	0.0
33	0.0	0.0	0.0	15.03450198647801	0.0
34	0.0	0.0	0.0	15.383006900397296	0.0
35	0.0	0.0	0.0	15.740473369245935	0.0
36	0.0	0.0	0.0	16.100927023071026	0.0
37	0.0	0.0	0.0	16.43051309880612	0.0
38	0.0	0.0	0.0	16.76308635951767	0.0
39	0.0	0.0	0.0	17.12851865497018	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACCG	20	7.002533E-4	45.000004	8
GCACCGA	20	7.002533E-4	45.000004	9
AGCGACT	20	7.002533E-4	45.000004	19
TTCATGC	20	7.002533E-4	45.000004	15
TGAGCGG	20	7.002533E-4	45.000004	2
TGTCGCA	20	7.002533E-4	45.000004	39
TCGCAGT	25	3.8653372E-5	45.0	41
CGAACGG	25	3.8653372E-5	45.0	2
CACGGGC	25	3.8653372E-5	45.0	4
GGCCGGT	25	3.8653372E-5	45.0	9
GGCCGAT	25	3.8653372E-5	45.0	8
CACGCCG	25	3.8653372E-5	45.0	26
GGGCCGA	25	3.8653372E-5	45.0	7
TAGTGGT	25	3.8653372E-5	45.0	15
CGCCGGT	25	3.8653372E-5	45.0	28
TGTACGG	25	3.8653372E-5	45.0	2
AGGCGAT	60	0.0	44.999996	7
TGCACGG	30	2.1456963E-6	44.999996	2
TTGGGAC	30	2.1456963E-6	44.999996	5
TTGCGGG	55	6.002665E-11	40.909092	3
>>END_MODULE