FastQCFastQC Report
Sat 18 Jun 2016
SRR3551394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157780
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.15464570921536316No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2430.15401191532513628No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC1790.11344910635061478No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGG207.014773E-445.02
TAAACGG207.014773E-445.02
TCGGGAT207.014773E-445.05
CACTCGG207.014773E-445.042
GTTCGTC207.014773E-445.021
CTAGGCA207.014773E-445.028
GTCGGGA207.014773E-445.04
TGGGTAT302.1535689E-644.9999966
CGAATAT650.041.5384614
AAGCTAC650.041.538468
CTCGTAA556.002665E-1140.90909233
GCTCGTA556.002665E-1140.90909232
CGAATAG501.0695658E-940.526
AACGAAT501.0695658E-940.524
TTACGGG501.0695658E-940.53
CGGGAGT451.9103027E-840.0000046
TCACGGG356.2140807E-638.571433
CGATTTG356.2140807E-638.5714310
TACGAAT700.038.5714312
GCGATTT356.2140807E-638.571439