##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	157780
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09890353656991	33.0	31.0	34.0	30.0	34.0
2	32.22809608315376	33.0	31.0	34.0	30.0	34.0
3	32.150703511218154	33.0	31.0	34.0	30.0	34.0
4	35.87533907973127	37.0	35.0	37.0	35.0	37.0
5	35.91786664976549	37.0	35.0	37.0	35.0	37.0
6	35.88732412219546	37.0	35.0	37.0	35.0	37.0
7	26.661439979718594	36.0	10.0	37.0	0.0	37.0
8	31.08229179870706	36.0	19.0	37.0	17.0	37.0
9	35.94378881987578	39.0	32.0	39.0	32.0	39.0
10	36.71078083407276	38.0	35.0	39.0	33.0	39.0
11	37.088205095702875	39.0	37.0	39.0	34.0	39.0
12	37.14053745721891	39.0	37.0	39.0	34.0	39.0
13	37.11552161237166	39.0	37.0	39.0	33.0	39.0
14	38.35792876156674	40.0	38.0	41.0	34.0	41.0
15	38.4402205602738	40.0	38.0	41.0	34.0	41.0
16	38.37602991507162	40.0	38.0	41.0	34.0	41.0
17	38.27880593231081	40.0	37.0	41.0	34.0	41.0
18	38.20321333502345	40.0	37.0	41.0	34.0	41.0
19	38.08591076182026	40.0	37.0	41.0	34.0	41.0
20	38.1410635061478	40.0	37.0	41.0	34.0	41.0
21	38.0684434022056	40.0	37.0	41.0	34.0	41.0
22	38.04652047154266	40.0	37.0	41.0	34.0	41.0
23	38.01234630498162	40.0	37.0	41.0	34.0	41.0
24	37.94876410191406	40.0	36.0	41.0	33.0	41.0
25	37.810894916973	40.0	36.0	41.0	33.0	41.0
26	37.67696159209025	40.0	36.0	41.0	33.0	41.0
27	37.57717708201293	40.0	36.0	41.0	33.0	41.0
28	37.54399163392065	40.0	36.0	41.0	33.0	41.0
29	37.52154265432881	40.0	36.0	41.0	33.0	41.0
30	37.45126124984155	40.0	36.0	41.0	33.0	41.0
31	37.32635314995564	40.0	36.0	41.0	32.0	41.0
32	37.16119913804031	40.0	35.0	41.0	32.0	41.0
33	37.07760172391938	39.0	35.0	41.0	31.0	41.0
34	36.923057421726455	39.0	35.0	41.0	31.0	41.0
35	36.83138547344404	39.0	35.0	41.0	31.0	41.0
36	36.68399670427177	39.0	35.0	41.0	30.0	41.0
37	36.57950944352896	39.0	35.0	41.0	30.0	41.0
38	36.454056280897454	39.0	35.0	41.0	30.0	41.0
39	36.40242109266067	39.0	35.0	41.0	30.0	41.0
40	36.237032577005955	39.0	35.0	41.0	29.0	41.0
41	36.14293319812397	39.0	35.0	40.0	29.0	41.0
42	36.06469768031436	39.0	35.0	40.0	29.0	41.0
43	35.99695145138801	39.0	35.0	40.0	29.0	41.0
44	35.81848776777792	38.0	35.0	40.0	28.0	41.0
45	35.67482570668019	38.0	35.0	40.0	28.0	41.0
46	35.564982887564966	38.0	34.0	40.0	27.0	41.0
47	35.48062492077576	38.0	34.0	40.0	27.0	41.0
48	35.38795157814679	38.0	34.0	40.0	27.0	41.0
49	35.2103371783496	38.0	34.0	40.0	26.0	41.0
50	34.99710356192166	38.0	34.0	40.0	26.0	41.0
51	32.97883762200532	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	11.0
10	12.0
11	12.0
12	10.0
13	9.0
14	6.0
15	9.0
16	23.0
17	42.0
18	68.0
19	124.0
20	201.0
21	279.0
22	439.0
23	650.0
24	890.0
25	1141.0
26	1533.0
27	1664.0
28	1912.0
29	2392.0
30	2874.0
31	3609.0
32	4829.0
33	6910.0
34	10833.0
35	13681.0
36	14989.0
37	24108.0
38	30863.0
39	33639.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93116998352136	18.703891494485994	23.353403473190518	16.01153504880213
2	29.437824819368743	25.75991887438205	27.019901128153123	17.782355178096083
3	32.53010520978577	24.10381543921917	27.185321333502344	16.18075801749271
4	29.835847382431236	25.42907846368361	25.90379008746356	18.8312840664216
5	26.051464063886424	31.346812016732155	23.646216250475348	18.955507668906073
6	26.86398783115731	35.9519584231208	23.147420458866776	14.036633286855116
7	61.49068322981367	28.966915958930155	5.506401318291291	4.035999492964888
8	83.7064266700469	6.079984788946635	6.899480289010014	3.3141082519964504
9	76.93116998352136	8.546710609709722	8.977056661173787	5.545062745595133
10	42.91608568893396	27.468627202433773	15.823932057294968	13.791355051337305
11	33.49473951071112	26.495753580935478	22.469894790214223	17.539612118139182
12	31.009633667131446	23.812270249714793	26.472303206997083	18.705792876156675
13	24.77753834453036	25.95639498035239	27.849537330460134	21.416529344657118
14	21.386741031816452	27.931296742299406	27.85460768158195	22.827354544302192
15	20.825199645075422	26.128786918494107	32.91418430726328	20.131829129167194
16	24.45050069717328	24.82063632906579	30.38217771580682	20.346685257954114
17	23.407275953859806	25.700342248700725	27.39067055393586	23.50171124350361
18	24.927113702623906	26.109773101787297	27.164406135124857	21.798707060463936
19	26.758144251489412	27.021802509823807	24.799087336798074	21.420965901888707
20	28.259601977436937	25.08429458740018	26.925465838509314	19.73063759665357
21	26.807580174927114	26.824058816073016	25.546964127265813	20.82139688173406
22	25.601470401825328	26.005197109899857	25.79731271390544	22.596019774369374
23	25.507668906071746	26.562301939409306	26.270756749904933	21.65927240461402
24	24.110153378121435	24.582963620230704	28.913677272151094	22.393205729496767
25	23.843326150335912	26.780327037647357	27.09405501330967	22.28229179870706
26	22.97692990239574	28.628470021548992	26.16047661300545	22.234123463049816
27	23.216503992901508	28.26150335910762	26.382304474584867	22.139688173406007
28	21.635822030675627	28.65382177715807	28.249461275193305	21.460894916973
29	22.853340093801496	27.2525034858664	27.68475091900114	22.209405501330966
30	23.43516288502979	26.472303206997083	27.36975535555837	22.722778552414756
31	25.340347319051844	27.126378501711244	25.089998732412223	22.443275446824693
32	25.736468500443653	26.945747242996575	26.353783749524656	20.964000507035113
33	24.646976803143616	27.8203828115097	25.46076815819496	22.071872227151733
34	23.558752693624033	27.4458106223856	28.038407909747747	20.957028774242616
35	23.691849410571685	26.531246038788186	27.403980225630626	22.372924325009507
36	24.454937254404868	29.524020788439596	24.98985929775637	21.031182659399164
37	24.288249461275193	29.325009506908355	26.55913296995817	19.827608061858285
38	22.514894156420333	28.60438585372037	26.614906832298136	22.265813157561162
39	23.746989479021423	27.497781721384207	26.952085181898845	21.803143617695525
40	24.510077322854606	26.55469641272658	27.393839523386994	21.54138674103182
41	21.152237292432503	27.473063759665354	28.208898466218784	23.16580048168336
42	23.54100646469768	26.730257320319435	26.396247940169857	23.33248827481303
43	24.09684370642667	26.61300545062746	26.092026872860945	23.19812397008493
44	23.864875142603626	27.012295601470406	26.351882367853975	22.770946888072
45	22.69108885790341	27.454049942958548	25.627455951324627	24.22740524781341
46	21.91025478514387	29.019520851818985	26.017239193814174	23.05298516922297
47	22.72404614019521	27.139688173406007	27.879325643300803	22.256940043097984
48	23.063759665356827	25.796045126124984	28.753961211813916	22.38623399670427
49	21.955887945240207	27.152364051210547	27.75636962859678	23.135378374952463
50	20.29027760172392	27.90721257447078	28.584738243123336	23.217771580681962
51	21.083153758397767	27.177715806819624	27.057928761566743	24.68120167321587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	177.0
2	315.0
3	484.0
4	653.0
5	491.0
6	329.0
7	283.5
8	238.0
9	256.0
10	274.0
11	286.5
12	299.0
13	312.0
14	325.0
15	338.5
16	352.0
17	358.0
18	364.0
19	435.5
20	507.0
21	533.5
22	560.0
23	616.5
24	673.0
25	849.0
26	1323.0
27	1621.0
28	2075.5
29	2530.0
30	2972.0
31	3414.0
32	3860.0
33	4306.0
34	4647.5
35	4989.0
36	5096.5
37	5204.0
38	5827.5
39	6451.0
40	7189.5
41	7928.0
42	8458.5
43	8989.0
44	9618.0
45	10247.0
46	10623.0
47	10999.0
48	11188.0
49	11377.0
50	11491.5
51	11606.0
52	10845.0
53	10084.0
54	9628.5
55	9173.0
56	8818.5
57	8464.0
58	8212.5
59	7961.0
60	7725.5
61	7490.0
62	6947.0
63	6404.0
64	5325.5
65	4247.0
66	3596.0
67	2945.0
68	2460.5
69	1976.0
70	1590.5
71	1205.0
72	1043.5
73	882.0
74	720.5
75	455.0
76	351.0
77	272.0
78	193.0
79	154.5
80	116.0
81	79.0
82	42.0
83	37.5
84	33.0
85	25.5
86	18.0
87	15.0
88	12.0
89	8.5
90	5.0
91	3.0
92	1.0
93	3.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	157780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.80415768791989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.4104000452668	28.858537203701356
2	11.712782210151078	10.495626822157435
3	6.986646296610649	9.39092407149195
4	4.77989022803146	8.566358220306757
5	3.281842358399819	7.352009126632019
6	2.288802127539184	6.152871086322728
7	1.7880382504385222	5.607808340727596
8	1.338199513381995	4.796552161237166
9	0.9647484863916709	3.8902268982127017
>10	2.4231879137667627	13.704525288376221
>50	0.01838963390482657	0.6249207757637216
>100	0.007072936117240989	0.5596400050703512
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	244	0.15464570921536316	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	243	0.15401191532513628	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	179	0.11344910635061478	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031055900621118012	0.0
2	0.0	0.0	0.0	0.19203954873875015	0.0
3	0.0	0.0	0.0	0.2478134110787172	0.0
4	0.0	0.0	0.0	0.34478387628343266	0.0
5	0.0	0.0	0.0	0.653441500823932	0.0
6	0.0	0.0	0.0	0.8822410951958423	0.0
7	0.0	0.0	0.0	1.0742806439345924	0.0
8	0.0	0.0	0.0	1.4608949169730003	0.0
9	0.0	0.0	0.0	1.6231461528710862	0.0
10	0.0	0.0	0.0	2.0579287615667385	0.0
11	0.0	0.0	0.0	2.4616554696412725	0.0
12	0.0	0.0	0.0	2.8476359487894536	0.0
13	0.0	0.0	0.0	2.976296108505514	0.0
14	0.0	0.0	0.0	3.034605146406389	0.0
15	0.0	0.0	0.0	3.144251489415642	0.0
16	0.0	0.0	0.0	3.3648117632146026	0.0
17	0.0	0.0	0.0	3.6462162504753453	0.0
18	0.0	0.0	0.0	3.94156420332108	0.0
19	0.0	0.0	0.0	4.117125110913931	0.0
20	0.0	0.0	0.0	4.288249461275194	0.0
21	0.0	0.0	0.0	4.501204208391431	0.0
22	0.0	0.0	0.0	4.754087970591963	0.0
23	0.0	0.0	0.0	4.978451007732286	0.0
24	0.0	0.0	0.0	5.162251235898086	0.0
25	0.0	0.0	0.0	5.337812143490937	0.0
26	0.0	0.0	0.0	5.506401318291291	0.0
27	0.0	0.0	0.0	5.669286348079605	0.0
28	0.0	0.0	0.0	5.8549879579160855	0.0
29	0.0	0.0	0.0	6.030548865508936	0.0
30	0.0	0.0	0.0	6.249841551527443	0.0
31	0.0	0.0	0.0	6.457725947521866	0.0
32	0.0	0.0	0.0	6.659906198504246	0.0
33	0.0	0.0	0.0	6.867790594498669	0.0
34	0.0	0.0	0.0	7.075041196602864	0.0
35	0.0	0.0	0.0	7.2943338826213715	0.0
36	0.0	0.0	0.0	7.510457599188744	0.0
37	0.0	0.0	0.0	7.733553048548612	0.0
38	0.0	0.0	0.0	7.974394726834833	0.0
39	0.0	0.0	0.0	8.25072886297376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	20	7.014773E-4	45.0	2
TAAACGG	20	7.014773E-4	45.0	2
TCGGGAT	20	7.014773E-4	45.0	5
CACTCGG	20	7.014773E-4	45.0	42
GTTCGTC	20	7.014773E-4	45.0	21
CTAGGCA	20	7.014773E-4	45.0	28
GTCGGGA	20	7.014773E-4	45.0	4
TGGGTAT	30	2.1535689E-6	44.999996	6
CGAATAT	65	0.0	41.53846	14
AAGCTAC	65	0.0	41.53846	8
CTCGTAA	55	6.002665E-11	40.909092	33
GCTCGTA	55	6.002665E-11	40.909092	32
CGAATAG	50	1.0695658E-9	40.5	26
AACGAAT	50	1.0695658E-9	40.5	24
TTACGGG	50	1.0695658E-9	40.5	3
CGGGAGT	45	1.9103027E-8	40.000004	6
TCACGGG	35	6.2140807E-6	38.57143	3
CGATTTG	35	6.2140807E-6	38.57143	10
TACGAAT	70	0.0	38.57143	12
GCGATTT	35	6.2140807E-6	38.57143	9
>>END_MODULE