FastQCFastQC Report
Sat 18 Jun 2016
SRR3551391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182065
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3230.17740916705572185No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.1340180704693379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA302.1554115E-645.0000041
CCTACTC207.017635E-445.032
CGACAAT207.017635E-445.020
ATATACT551.8189894E-1245.017
ACCACTA253.877838E-545.022
TCATCGA207.017635E-445.016
CCGGTAT207.017635E-445.027
TTCATCG253.877838E-545.015
TATACTG551.8189894E-1245.018
TCGACAA207.017635E-445.019
GAATATA603.6379788E-1241.25000415
CGAATAT603.6379788E-1241.25000414
TAGGGCG501.0713848E-940.55
CGTTAGG356.2193667E-638.5714262
CGTTTTT1650.038.1818161
TACGAAT659.094947E-1238.0769212
AGCTACG659.094947E-1238.076929
GGGTCTC301.1361517E-437.5000047
CGTTGAT301.1361517E-437.50000425
ATCGTTG301.1361517E-437.50000423