##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551390_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64652
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976876198725485	33.0	31.0	34.0	30.0	34.0
2	32.119068242281756	33.0	31.0	34.0	30.0	34.0
3	31.970457217100787	33.0	31.0	34.0	30.0	34.0
4	35.74588566478995	37.0	35.0	37.0	35.0	37.0
5	35.82178432221741	37.0	35.0	37.0	35.0	37.0
6	35.790462785374004	37.0	35.0	37.0	35.0	37.0
7	26.7610437418796	35.0	10.0	37.0	0.0	37.0
8	31.127637196065088	35.0	19.0	37.0	17.0	37.0
9	35.94977726907133	39.0	32.0	39.0	32.0	39.0
10	36.727324754067936	38.0	35.0	39.0	33.0	39.0
11	37.05497123058838	39.0	37.0	39.0	34.0	39.0
12	36.913521623460994	39.0	35.0	39.0	33.0	39.0
13	36.66678525026295	39.0	35.0	39.0	33.0	39.0
14	37.81685021345047	40.0	36.0	41.0	33.0	41.0
15	37.981299882447566	40.0	36.0	41.0	33.0	41.0
16	37.98793540803069	40.0	36.0	41.0	33.0	41.0
17	37.93172678339417	40.0	36.0	41.0	33.0	41.0
18	37.83358596795149	40.0	36.0	41.0	33.0	41.0
19	37.71176452391264	39.0	36.0	41.0	33.0	41.0
20	37.72970673761059	39.0	35.0	41.0	33.0	41.0
21	37.65631380313061	39.0	35.0	41.0	33.0	41.0
22	37.64944626616346	39.0	35.0	41.0	33.0	41.0
23	37.622950566107775	39.0	35.0	41.0	33.0	41.0
24	37.53070283981934	39.0	35.0	41.0	33.0	41.0
25	37.446358968013364	39.0	35.0	41.0	33.0	41.0
26	37.29703644125472	39.0	35.0	41.0	33.0	41.0
27	37.177999133824166	39.0	35.0	41.0	32.0	41.0
28	37.19038854173112	39.0	35.0	41.0	32.0	41.0
29	37.15456598403762	39.0	35.0	41.0	32.0	41.0
30	37.07730619315721	39.0	35.0	41.0	32.0	41.0
31	36.91537771453319	39.0	35.0	41.0	32.0	41.0
32	36.70969188888201	39.0	35.0	41.0	31.0	41.0
33	36.578357978098126	39.0	35.0	41.0	31.0	41.0
34	36.439584235599824	39.0	35.0	41.0	30.0	41.0
35	36.33785497741756	39.0	35.0	41.0	30.0	41.0
36	36.173946668316525	38.0	35.0	40.0	30.0	41.0
37	36.02869207449112	38.0	35.0	40.0	29.0	41.0
38	35.9191362989544	38.0	35.0	40.0	29.0	41.0
39	35.891526944255396	38.0	35.0	40.0	29.0	41.0
40	35.677349501948896	38.0	35.0	40.0	28.0	41.0
41	35.62061498484192	38.0	35.0	40.0	28.0	41.0
42	35.577166986326795	38.0	35.0	40.0	28.0	41.0
43	35.51692136360824	38.0	35.0	40.0	28.0	41.0
44	35.34756852069542	38.0	34.0	40.0	27.0	41.0
45	35.21159438223102	38.0	34.0	40.0	26.0	41.0
46	35.095464950813586	38.0	34.0	40.0	26.0	41.0
47	34.945338117923654	37.0	34.0	40.0	26.0	41.0
48	34.87854977417559	37.0	34.0	40.0	26.0	41.0
49	34.7150745529914	37.0	34.0	40.0	25.0	41.0
50	34.465569510610656	37.0	34.0	40.0	24.0	41.0
51	32.40137969436367	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	10.0
11	6.0
12	3.0
13	3.0
14	2.0
15	5.0
16	5.0
17	9.0
18	18.0
19	47.0
20	99.0
21	133.0
22	206.0
23	288.0
24	428.0
25	595.0
26	721.0
27	908.0
28	946.0
29	1133.0
30	1324.0
31	1707.0
32	2235.0
33	3132.0
34	5324.0
35	6956.0
36	5827.0
37	9020.0
38	11417.0
39	12141.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.69640537029017	20.04578357978098	24.69374497308668	16.56406607684217
2	30.229536595928973	26.22502010765328	26.574583926251314	16.97085937016643
3	31.46847738662377	25.312441997153996	27.556765451958178	15.66231516426406
4	30.769349749427704	24.636515498360453	26.670481964981747	17.923652787230093
5	25.689847181835056	30.062488399430798	25.03093485120337	19.21672956753078
6	26.18944502876941	36.79855224896368	23.982243395409267	13.029759326857636
7	60.445152508816435	28.54668069046588	7.009837282682669	3.998329518035018
8	82.11192229165377	6.09880591474355	8.070902678958113	3.7183691146445583
9	75.02320113840253	8.899956691208315	10.053826641093856	6.0230155292953045
10	45.65829363360762	25.907937882818782	16.341335148177937	12.092433335395656
11	39.5826888572666	22.93355193961517	21.55385757594506	15.929901627173173
12	37.37084699622595	21.332673389840995	24.72158633916971	16.574893274763348
13	25.18560910722019	29.12052218028831	26.266782156777822	19.427086555713664
14	17.442615851017756	33.886036008166805	27.41601187898286	21.255336261832582
15	17.363731980449174	24.781909299016274	40.50145393800656	17.352904782527993
16	19.337375487223905	22.74484934727464	39.08773123801274	18.83004392748871
17	19.55855967332797	22.48035636948586	29.607746086741322	28.353337870444843
18	22.44787477572233	25.97908804058652	29.25044855534245	22.322588628348697
19	27.49798923467178	26.965909793973893	25.788838705685823	19.747262265668503
20	31.301429190125596	24.00853801893213	26.23584730557446	18.454185485367816
21	24.294685392563263	27.21029511848048	26.57613066881148	21.918888820144776
22	22.024067314236216	26.8143290230774	26.178617830848232	24.982985831838146
23	23.77343314978655	28.08575140753573	25.462476025490314	22.678339417187406
24	23.965229227247416	24.526696776588505	30.19241477448493	21.31565922167914
25	21.70853183196189	26.320918146383715	28.13988739714162	23.830662624512776
26	21.11148920373693	30.74614861102518	26.99684464517726	21.14551754006063
27	22.183381797933553	29.278289921425475	28.05017632865186	20.48815195198911
28	20.384520200457835	28.15690156530347	31.54272102951185	19.915857204726844
29	22.064282620800594	26.276062612138833	30.571366701726166	21.088288065334407
30	22.141619748809006	27.866113963991836	27.66967765885046	22.322588628348697
31	25.753263626801953	27.153065643754253	24.175586215430304	22.918084514013486
32	26.204912454371094	29.95266967765885	24.46018684650127	19.382231021468787
33	25.573841489822435	30.59147435500835	23.66670791313494	20.167976242034275
34	23.190311204603105	28.920992390026605	26.232753820454125	21.655942584916165
35	22.497370537647715	26.025490317391576	26.582317639052157	24.894821505908556
36	24.3952236589742	28.453876136855783	26.42454989791499	20.726350306255025
37	22.910350801212644	29.38501515807709	27.863020478871498	19.841613561838766
38	22.472622656685022	29.368000989915238	24.464827074181773	23.694549279217966
39	22.746396089834807	27.378890057538825	27.62482212460558	22.24989172802079
40	24.35964858009033	26.324011631504053	28.01460124976799	21.30173853863763
41	18.551630266658417	28.39974014724989	28.760131163769103	24.288498422322586
42	23.19649817484378	27.295365959289736	25.696034152075732	23.812101713790756
43	24.372022520571676	27.33712800841428	25.502691332054695	22.788158138959353
44	22.314854915547855	29.34170636639238	26.59623832209367	21.747200395966097
45	21.239868836230897	28.933366330507948	25.309348512033658	24.517416321227493
46	22.341149539070717	30.357916228422944	24.608674132277425	22.692260100228918
47	21.821444038854175	28.269813772195757	27.87694116191301	22.03180102703706
48	23.705376477139144	24.83140506094166	27.648023263008103	23.815195198911095
49	20.565489079997526	26.370413908309096	28.198663614428014	24.86543339726536
50	18.771267710202313	28.325496504361812	28.808080183134322	24.095155602301553
51	20.39844088349935	28.80189321289365	24.58392625131473	26.215739652292275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	99.0
2	178.0
3	294.0
4	410.0
5	307.5
6	205.0
7	180.0
8	155.0
9	169.5
10	184.0
11	190.5
12	197.0
13	185.5
14	174.0
15	170.5
16	167.0
17	157.0
18	147.0
19	178.0
20	209.0
21	219.5
22	230.0
23	275.0
24	320.0
25	429.5
26	581.0
27	623.0
28	790.5
29	958.0
30	1114.0
31	1270.0
32	1388.5
33	1507.0
34	1706.5
35	1906.0
36	1830.5
37	1755.0
38	2062.5
39	2370.0
40	2906.0
41	3442.0
42	4012.5
43	4583.0
44	4868.5
45	5154.0
46	5362.0
47	5570.0
48	5107.0
49	4644.0
50	4649.0
51	4654.0
52	4269.5
53	3885.0
54	3615.0
55	3345.0
56	3195.5
57	3046.0
58	3098.5
59	3151.0
60	3083.5
61	3016.0
62	2773.0
63	2530.0
64	2073.5
65	1617.0
66	1275.5
67	934.0
68	777.5
69	621.0
70	488.0
71	355.0
72	302.5
73	250.0
74	192.5
75	117.0
76	99.0
77	69.5
78	40.0
79	32.0
80	24.0
81	16.0
82	8.0
83	11.0
84	14.0
85	10.5
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	64652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.73396027965106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.59177707283696	34.113407164511536
2	11.441811283199602	11.380931757718244
3	6.31336692168937	9.419662191424859
4	3.8595509112396593	7.678030068675369
5	2.8798905268395845	7.161418053579162
6	2.06506188965603	6.16222235971045
7	1.4057349007899482	4.893893460372455
8	1.1413820986502459	4.541236156654087
9	0.765068109721963	3.424488028212584
>10	1.5052559557131306	9.62692569448741
>50	0.01866019779809666	0.655818845511353
>100	0.012440131865397773	0.9419662191424859
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.2784136608302914	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	159	0.2459320670667574	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	146	0.22582441378456972	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	124	0.1917960774608674	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	93	0.14384705809565057	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	88	0.13611334529480915	No Hit
GCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAGAT	65	0.10053826641093858	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027841366083029142	0.0
2	0.0	0.0	0.0	0.3124419971539937	0.0
3	0.0	0.0	0.0	0.4624760254903174	0.0
4	0.0	0.0	0.0	0.6635525583121945	0.0
5	0.0	0.0	0.0	1.4168161851141496	0.0
6	0.0	0.0	0.0	1.78648765699437	0.0
7	0.0	0.0	0.0	2.170079811916105	0.0
8	0.0	0.0	0.0	2.941904349440079	0.0
9	0.0	0.0	0.0	3.2419724061127266	0.0
10	0.0	0.0	0.0	4.199406050856895	0.0
11	0.0	0.0	0.0	5.094969993194333	0.0
12	0.0	0.0	0.0	6.058590608179174	0.0
13	0.0	0.0	0.0	6.347831466930644	0.0
14	0.0	0.0	0.0	6.482398069665285	0.0
15	0.0	0.0	0.0	6.700488770649013	0.0
16	0.0	0.0	0.0	7.124296232135124	0.0
17	0.0	0.0	0.0	7.5852255150652725	0.0
18	0.0	0.0	0.0	8.142052836725856	0.0
19	0.0	0.0	0.0	8.423559982676483	0.0
20	0.0	0.0	0.0	8.709707356307616	0.0
21	0.0	0.0	0.0	9.122687619872549	0.0
22	0.0	0.0	0.0	9.504733032234116	0.0
23	0.0	0.0	0.0	9.903792612757533	0.0
24	0.0	0.0	0.0	10.228608550392872	0.0
25	0.0	0.0	0.0	10.514755924024005	0.0
26	0.0	0.0	0.0	10.765328218771268	0.0
27	0.0	0.0	0.0	11.023634226319372	0.0
28	0.0	0.0	0.0	11.308234857390335	0.0
29	0.0	0.0	0.0	11.591288745901132	0.0
30	0.0	0.0	0.0	11.934665594258492	0.0
31	0.0	0.0	0.0	12.299696838458207	0.0
32	0.0	0.0	0.0	12.647713914496071	0.0
33	0.0015467425601682855	0.0	0.0	12.926127575326362	0.0
34	0.0015467425601682855	0.0	0.0	13.210728206397327	0.0
35	0.0015467425601682855	0.0	0.0	13.609787786920744	0.0
36	0.0015467425601682855	0.0	0.0	13.967085318319619	0.0
37	0.0015467425601682855	0.0	0.0	14.339850275320176	0.0
38	0.0015467425601682855	0.0	0.0	14.647652044793665	0.0
39	0.0015467425601682855	0.0	0.0	14.989482150590856	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCATA	20	6.983944E-4	45.000004	38
CGACAAT	20	6.983944E-4	45.000004	20
GCGGGAT	20	6.983944E-4	45.000004	5
AACGCGG	20	6.983944E-4	45.000004	2
ACCGGCG	20	6.983944E-4	45.000004	20
ACTCGCC	20	6.983944E-4	45.000004	36
GTCATAC	20	6.983944E-4	45.000004	39
TCGACAA	20	6.983944E-4	45.000004	19
GAAATAG	20	6.983944E-4	45.000004	34
TGCCGGC	20	6.983944E-4	45.000004	27
CACGCCA	20	6.983944E-4	45.000004	27
CACCGGC	20	6.983944E-4	45.000004	19
AGTGGGA	20	6.983944E-4	45.000004	4
AGGCGTC	20	6.983944E-4	45.000004	35
ACACCGG	20	6.983944E-4	45.000004	18
ATGAAAC	20	6.983944E-4	45.000004	3
AGGCGAT	20	6.983944E-4	45.000004	7
AGAACTT	20	6.983944E-4	45.000004	39
TGGGGTC	20	6.983944E-4	45.000004	6
CGGCGCA	20	6.983944E-4	45.000004	22
>>END_MODULE