FastQCFastQC Report
Sat 18 Jun 2016
SRR3551385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967514
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC124861.2905239614103776No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC121551.2563125701540234No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG113891.1771405891801048No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC75150.776732946499999No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC49640.513067511167797No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT32260.3334318676525611No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT25750.26614601959248135No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT23110.23885959272940752No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.21260674264145016No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCCCTT19860.20526834753812348No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTA18950.19586279888456395No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC18000.18604381952095783No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC16720.1728140367994675No Hit
TGTGCGGGGCAATGAGTTTGGCCACCTAAATAGTGTTGCATAAAATTATAT14670.15162571290958063No Hit
ACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC14290.1476981211641382No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG12180.12588965120918147No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11440.11824118307331988No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC10590.10945578048483018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.2116652E-745.0000041
TACGGGT453.8380676E-1045.0000044
TAACGAT207.032731E-445.00000422
GCGTTAC207.032731E-445.00000427
CGCCTAT207.032731E-445.0000043
ACGATAG406.8139343E-945.0000041
AACGATA207.032731E-445.00000423
ACGTAGT207.032731E-445.00000429
CGCAATA207.032731E-445.00000412
TAGGACG406.8139343E-945.0000041
GACTCGT207.032731E-445.00000415
AGCGATC253.890346E-545.024
CGGGTAC650.044.9999966
TGCTACG302.165145E-644.9999961
ATAACGA302.165145E-644.99999644
TAGTGCG600.044.9999961
TCACGAC603.6379788E-1241.24999625
AGTACGG603.6379788E-1241.2499962
CCTAAAT2250.041.025
CATACGA2250.041.018
Produced by FastQC (version 0.11.5)