##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	967514
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97109602548387	33.0	31.0	34.0	30.0	34.0
2	32.01060759844302	33.0	31.0	34.0	30.0	34.0
3	32.05029487945394	33.0	31.0	34.0	30.0	34.0
4	35.72921735499435	37.0	35.0	37.0	33.0	37.0
5	34.82183720338931	37.0	35.0	37.0	33.0	37.0
6	35.28234113408178	37.0	35.0	37.0	33.0	37.0
7	35.80732165115957	37.0	35.0	37.0	35.0	37.0
8	35.72910262797231	37.0	35.0	37.0	35.0	37.0
9	37.59131444092799	39.0	37.0	39.0	35.0	39.0
10	37.329054670009945	39.0	37.0	39.0	34.0	39.0
11	37.293853112203024	39.0	37.0	39.0	34.0	39.0
12	37.22326912065355	39.0	37.0	39.0	34.0	39.0
13	37.11862257290334	39.0	37.0	39.0	33.0	39.0
14	38.23213617580728	40.0	37.0	41.0	33.0	41.0
15	38.27034337487623	40.0	37.0	41.0	34.0	41.0
16	38.342089106720934	40.0	37.0	41.0	34.0	41.0
17	38.26190732123773	40.0	37.0	41.0	34.0	41.0
18	38.264740355178326	40.0	37.0	41.0	34.0	41.0
19	38.22169498322505	40.0	37.0	41.0	34.0	41.0
20	38.17270241050775	40.0	37.0	41.0	34.0	41.0
21	38.16155321783457	40.0	37.0	41.0	34.0	41.0
22	38.195418360871265	40.0	37.0	41.0	34.0	41.0
23	38.19786070279086	40.0	37.0	41.0	34.0	41.0
24	38.139780923066745	40.0	37.0	41.0	34.0	41.0
25	38.01378274629618	40.0	37.0	41.0	34.0	41.0
26	38.01103756638147	40.0	37.0	41.0	34.0	41.0
27	38.004964269250884	40.0	37.0	41.0	34.0	41.0
28	37.91445085032361	40.0	36.0	41.0	33.0	41.0
29	37.901326492433185	40.0	36.0	41.0	34.0	41.0
30	37.79295493398545	40.0	36.0	41.0	33.0	41.0
31	37.77241362915679	40.0	36.0	41.0	33.0	41.0
32	37.70642905425658	40.0	36.0	41.0	33.0	41.0
33	37.58069340598689	40.0	36.0	41.0	33.0	41.0
34	37.489547438073245	40.0	36.0	41.0	33.0	41.0
35	37.487206386677606	40.0	36.0	41.0	33.0	41.0
36	37.42739123154807	40.0	36.0	41.0	33.0	41.0
37	37.37719350831099	40.0	36.0	41.0	33.0	41.0
38	37.2866976601889	40.0	36.0	41.0	33.0	41.0
39	37.23720897061955	40.0	35.0	41.0	33.0	41.0
40	37.129463759697536	39.0	35.0	41.0	32.0	41.0
41	37.021557310798606	39.0	35.0	41.0	32.0	41.0
42	37.091175941640124	39.0	35.0	41.0	32.0	41.0
43	36.96483565095699	39.0	35.0	41.0	32.0	41.0
44	36.87805241061111	39.0	35.0	41.0	31.0	41.0
45	36.854459987142306	39.0	35.0	41.0	31.0	41.0
46	36.71362791649526	39.0	35.0	41.0	31.0	41.0
47	36.60024557784177	39.0	35.0	41.0	31.0	41.0
48	36.572482672085364	39.0	35.0	41.0	31.0	41.0
49	36.60065384066794	39.0	35.0	41.0	31.0	41.0
50	36.46900509966781	39.0	35.0	41.0	31.0	41.0
51	35.279552543942515	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	15.0
10	24.0
11	33.0
12	13.0
13	12.0
14	20.0
15	23.0
16	50.0
17	91.0
18	179.0
19	354.0
20	716.0
21	1159.0
22	2031.0
23	2938.0
24	3764.0
25	4842.0
26	6238.0
27	7333.0
28	8504.0
29	10418.0
30	13197.0
31	17467.0
32	23361.0
33	33021.0
34	56968.0
35	75107.0
36	71489.0
37	105717.0
38	188781.0
39	333449.0
40	197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79003507959575	20.332935750800505	22.349960827440224	11.527068342163524
2	30.46178143158652	29.606910080887715	22.212805189382273	17.71850329814349
3	30.079771455503483	28.352561306606418	25.556529414561442	16.011137823328657
4	27.8020783161794	23.587358942609615	29.678536951403288	18.932025789807692
5	27.249011383814604	29.36236581589517	23.583638066219198	19.80498473407103
6	24.353652763680937	36.464175195397694	24.723466533817597	14.458705507103772
7	72.24618971921853	7.106047044280496	14.830999861500713	5.816763375000258
8	70.41231444712945	10.493078136337045	11.804790421637309	7.2898169948961975
9	63.73117081509931	9.019197655021012	14.032975233433314	13.216656296446356
10	37.31274172776828	24.288640784526116	22.291667097323657	16.106950390381947
11	30.148711026403753	24.95188700111833	25.29834193613736	19.60106003634056
12	26.201791395266632	21.0768009558518	30.80151811756729	21.919889531314276
13	23.038116244312743	24.406675252244412	33.701321117833956	18.853887385608893
14	19.177086843187798	29.53848729837501	28.759377125292247	22.52504873314495
15	17.53297626701009	25.069508038126582	37.26540391146795	20.13211178339538
16	18.938330608135903	25.608104895639755	30.345193971353385	25.10837052487096
17	18.20779854348361	25.985670491589786	31.273139200052917	24.533391764873684
18	19.236724223112017	26.28964542115153	30.964513175003148	23.509117180733302
19	20.794117707857456	27.608696101555118	27.866263433914135	23.73092275667329
20	23.308913359393248	26.65532488418772	30.38209266222504	19.653669094193983
21	21.87430879553164	29.499831526985652	28.82955698832265	19.796302689160054
22	18.648515680393256	26.07765882457515	28.389046566768027	26.884778928263568
23	20.331695458670364	27.46771622942924	28.864181810289054	23.336406501611346
24	22.384585649406624	24.836436475337827	28.16217646462997	24.616801410625584
25	19.42421505011814	29.685771988829103	26.70814065739617	24.181872303656586
26	18.887892061510218	26.552173922031102	30.166281831580733	24.393652184877944
27	21.900251572587063	26.477446321190186	28.827076404062367	22.795225702160383
28	18.411103095149013	26.613051594085462	31.69752582391573	23.278319486849803
29	20.785332305268966	22.98250981381148	32.89265064898286	23.339507231936697
30	20.128907695392524	25.576684161676212	31.572773107159176	22.721635035772092
31	23.780431084201364	24.803155303179075	26.190111977707815	25.226301634911742
32	22.116682549296442	25.755182870738818	29.121335711938016	23.006798868026713
33	20.430402040693984	23.357078037113673	28.038147251615996	28.174372670576343
34	19.75837042151328	22.877084982749604	30.61030641417075	26.754238181566365
35	18.316220747193324	21.536225832391057	31.318926651190576	28.828626769225046
36	21.038661972850008	23.623740845093714	31.97142366932158	23.3661735127347
37	19.56478149153397	22.962561782051733	35.064298811179995	22.4083579152343
38	20.738407919678682	23.911695334641152	28.048276304012138	27.301620441668028
39	21.634208910672093	21.4522993982516	30.987251864055715	25.92623982702059
40	19.328195767709822	21.522169188249475	31.241098320024307	27.908536724016397
41	17.79602155627722	23.164005895521925	30.485346982059173	28.554625566141677
42	19.630930405141424	22.232959936497043	32.2334353818136	25.902674276547938
43	22.48122507788001	21.192044766277284	32.02299915039989	24.303731005442817
44	20.959386634198577	22.147586494872424	30.350361855228968	26.542665015700027
45	20.310300419425456	21.075664021399174	28.6746238297327	29.93941172944267
46	23.040390113218002	23.118115086706755	29.24526156727448	24.596233232800767
47	17.838604919412017	21.3590707731361	35.709044003497624	25.09328030395426
48	19.197655021012615	22.081851011975022	31.080480489171215	27.640013477841148
49	19.77676808811035	19.87599145852153	35.20145444923794	25.145786004130173
50	19.896559636346346	19.573256821089927	32.169663694788916	28.36051984777481
51	18.694509846885936	19.786897140506493	28.944387368038083	32.57420564456948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	628.0
1	1018.0
2	1408.0
3	2687.0
4	3966.0
5	2819.0
6	1672.0
7	1666.0
8	1660.0
9	1678.0
10	1696.0
11	1782.5
12	1869.0
13	1886.5
14	1904.0
15	1947.5
16	1991.0
17	2094.0
18	2197.0
19	2360.5
20	2524.0
21	3279.5
22	4035.0
23	3829.5
24	3624.0
25	5086.0
26	7735.5
27	8923.0
28	11071.5
29	13220.0
30	14492.0
31	15764.0
32	17720.5
33	19677.0
34	22144.0
35	24611.0
36	25591.0
37	26571.0
38	28393.5
39	30216.0
40	35957.5
41	41699.0
42	46736.5
43	51774.0
44	58705.0
45	65636.0
46	99420.0
47	133204.0
48	122135.5
49	111067.0
50	108320.5
51	105574.0
52	86008.0
53	66442.0
54	55859.0
55	45276.0
56	40783.5
57	36291.0
58	34464.5
59	32638.0
60	31057.5
61	29477.0
62	26719.5
63	23962.0
64	20543.0
65	17124.0
66	14164.5
67	11205.0
68	9308.0
69	7411.0
70	6362.0
71	5313.0
72	4642.5
73	3972.0
74	3101.5
75	1766.5
76	1302.0
77	945.5
78	589.0
79	406.0
80	223.0
81	180.0
82	137.0
83	156.0
84	175.0
85	118.0
86	61.0
87	34.0
88	7.0
89	10.5
90	14.0
91	9.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	967514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.022359825233472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.18482064633268	18.695371179494437
2	12.850013396936404	6.944753715421724
3	5.083757024787382	4.121253347635891
4	2.584076455274903	2.793113751614091
5	1.6064055956541785	2.170443501551786
6	1.0140792399584484	1.6441688468073885
7	0.7206207781016971	1.363101177438265
8	0.5552025871210804	1.20023072680691
9	0.4370886319500876	1.0630049659266876
>10	5.099959194976142	33.52017839430829
>50	0.7645979751722722	13.124416121038465
>100	0.08589689784050598	4.55005280851623
>500	0.006548194005778472	1.2794356298275482
>1k	0.005392630357699918	2.9980137037034553
>5k	3.8518788269285126E-4	0.7822127203184206
>10k+	0.0011555636480785538	3.7502494095905115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC	12486	1.2905239614103776	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	12155	1.2563125701540234	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG	11389	1.1771405891801048	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	7515	0.776732946499999	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	4964	0.513067511167797	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	3226	0.3334318676525611	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	2575	0.26614601959248135	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	2311	0.23885959272940752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2057	0.21260674264145016	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCCCTT	1986	0.20526834753812348	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTA	1895	0.19586279888456395	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC	1800	0.18604381952095783	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	1672	0.1728140367994675	No Hit
TGTGCGGGGCAATGAGTTTGGCCACCTAAATAGTGTTGCATAAAATTATAT	1467	0.15162571290958063	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	1429	0.1476981211641382	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	1218	0.12588965120918147	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1144	0.11824118307331988	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC	1059	0.10945578048483018	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.067153550232865E-4	0.0	0.0	0.35038252676447057	0.0
2	2.067153550232865E-4	0.0	0.0	2.045758510987955	0.0
3	2.067153550232865E-4	0.0	0.0	2.736911300508313	0.0
4	2.067153550232865E-4	0.0	0.0	3.745372159989416	0.0
5	2.067153550232865E-4	0.0	0.0	7.002069220703783	0.0
6	2.067153550232865E-4	0.0	0.0	8.254970987499922	0.0
7	2.067153550232865E-4	0.0	0.0	9.643788100223873	0.0
8	2.067153550232865E-4	0.0	0.0	11.536783963849619	0.0
9	2.067153550232865E-4	0.0	0.0	12.225662884464722	0.0
10	2.067153550232865E-4	0.0	0.0	14.895598409945489	0.0
11	2.067153550232865E-4	0.0	0.0	16.969780282249147	0.0
12	2.067153550232865E-4	0.0	0.0	19.960021250338496	0.0
13	2.067153550232865E-4	0.0	0.0	20.747916826009753	0.0
14	2.067153550232865E-4	0.0	0.0	21.092614680511083	0.0
15	2.067153550232865E-4	0.0	0.0	21.838443681435102	0.0
16	2.067153550232865E-4	0.0	0.0	22.93269141325087	0.0
17	2.067153550232865E-4	0.0	0.0	24.07065944265406	0.0
18	2.067153550232865E-4	0.0	0.0	25.328212304938223	0.0
19	2.067153550232865E-4	0.0	0.0	26.28416746424341	0.0
20	3.1007303253492973E-4	0.0	0.0	27.049427708539618	0.0
21	3.1007303253492973E-4	0.0	0.0	27.889932342064302	0.0
22	3.1007303253492973E-4	0.0	0.0	28.857566918928306	0.0
23	3.1007303253492973E-4	0.0	0.0	29.711921481239546	0.0
24	3.1007303253492973E-4	0.0	0.0	30.422608871809608	0.0
25	4.13430710046573E-4	0.0	0.0	31.019396101761835	0.0
26	4.13430710046573E-4	0.0	0.0	31.562850770118054	0.0
27	4.13430710046573E-4	0.0	0.0	32.163462234138215	0.0
28	4.13430710046573E-4	0.0	0.0	32.72521121141399	0.0
29	4.13430710046573E-4	0.0	0.0	33.29791610250601	0.0
30	4.13430710046573E-4	0.0	0.0	33.94793253637673	0.0
31	5.167883875582162E-4	0.0	0.0	34.53190341431752	0.0
32	5.167883875582162E-4	0.0	0.0	35.10388480166695	0.0
33	5.167883875582162E-4	0.0	0.0	35.644238739697826	0.0
34	5.167883875582162E-4	0.0	0.0	36.21539326562716	0.0
35	5.167883875582162E-4	0.0	0.0	36.76535946766662	0.0
36	5.167883875582162E-4	0.0	0.0	37.323697641584516	0.0
37	7.235037425815027E-4	0.0	0.0	37.84079610217527	0.0
38	7.235037425815027E-4	0.0	0.0	38.39830741467307	0.0
39	7.235037425815027E-4	0.0	0.0	38.927498723532686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2116652E-7	45.000004	1
TACGGGT	45	3.8380676E-10	45.000004	4
TAACGAT	20	7.032731E-4	45.000004	22
GCGTTAC	20	7.032731E-4	45.000004	27
CGCCTAT	20	7.032731E-4	45.000004	3
ACGATAG	40	6.8139343E-9	45.000004	1
AACGATA	20	7.032731E-4	45.000004	23
ACGTAGT	20	7.032731E-4	45.000004	29
CGCAATA	20	7.032731E-4	45.000004	12
TAGGACG	40	6.8139343E-9	45.000004	1
GACTCGT	20	7.032731E-4	45.000004	15
AGCGATC	25	3.890346E-5	45.0	24
CGGGTAC	65	0.0	44.999996	6
TGCTACG	30	2.165145E-6	44.999996	1
ATAACGA	30	2.165145E-6	44.999996	44
TAGTGCG	60	0.0	44.999996	1
TCACGAC	60	3.6379788E-12	41.249996	25
AGTACGG	60	3.6379788E-12	41.249996	2
CCTAAAT	225	0.0	41.0	25
CATACGA	225	0.0	41.0	18
>>END_MODULE