FastQCFastQC Report
Sat 18 Jun 2016
SRR3551383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461345
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC23400.5072126066176073No Hit
CCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC20220.4382837139234196No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT16410.3556990972049117No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.26531120961536375No Hit
TCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC9030.19573204434858946No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7960.17253898925966465No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA7120.15433135722723773No Hit
ACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC6540.14175942082389537No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCC5720.12398530383985955No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5520.11965015335594836No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5440.11791609316238388No Hit
GGTCTTGGGGGATGGACGTGCCAATCAATGGAACAGAATTGAAACTTCAGA4900.10621118685582374No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT4640.1005754912267392No Hit
CGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG4620.10014197617834808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA302.1626656E-645.00000415
TACGAAG302.1626656E-645.0000041
ACAACGA351.2098099E-745.013
GTACCGT207.0288905E-445.044
CGACGGT253.887163E-545.028
CTATCGA207.0288905E-445.01
TTAGCAC207.0288905E-445.028
TAGGTCG207.0288905E-445.01
CGTGAGA551.8189894E-1245.02
CGTTGAA207.0288905E-445.016
CAACCGG207.0288905E-445.02
CGACCGC207.0288905E-445.025
CACGACG253.887163E-545.026
TACGGGT253.887163E-545.04
GCCGCAC207.0288905E-445.027
CTAACGG207.0288905E-445.02
ATATCAC551.8189894E-1245.030
TCGTGCG207.0288905E-445.045
TTCCAAT207.0288905E-445.015
CTACGGA207.0288905E-445.011