##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551383_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461345
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.021649741516654	33.0	31.0	34.0	30.0	34.0
2	32.040160834082954	33.0	31.0	34.0	30.0	34.0
3	32.083822302181666	33.0	31.0	34.0	30.0	34.0
4	35.75189717023052	37.0	35.0	37.0	33.0	37.0
5	34.79903542901733	37.0	35.0	37.0	33.0	37.0
6	35.308909818032056	37.0	35.0	37.0	33.0	37.0
7	35.886295505532736	37.0	35.0	37.0	35.0	37.0
8	35.86370070121059	37.0	35.0	37.0	35.0	37.0
9	37.69918174034616	39.0	38.0	39.0	35.0	39.0
10	37.43237707138909	39.0	37.0	39.0	35.0	39.0
11	37.366578157344286	39.0	37.0	39.0	35.0	39.0
12	37.248167857026736	39.0	37.0	39.0	34.0	39.0
13	37.145184189706185	39.0	37.0	39.0	33.0	39.0
14	38.23872373169753	40.0	37.0	41.0	33.0	41.0
15	38.39565401163988	40.0	38.0	41.0	34.0	41.0
16	38.39882300664362	40.0	37.0	41.0	34.0	41.0
17	38.39982009125492	40.0	37.0	41.0	34.0	41.0
18	38.38742806359666	40.0	37.0	41.0	34.0	41.0
19	38.282346183441895	40.0	37.0	41.0	34.0	41.0
20	38.29562041422363	40.0	37.0	41.0	34.0	41.0
21	38.19647335508134	40.0	37.0	41.0	34.0	41.0
22	38.23459450086161	40.0	37.0	41.0	34.0	41.0
23	38.237613933173655	40.0	37.0	41.0	34.0	41.0
24	38.18776403775916	40.0	37.0	41.0	34.0	41.0
25	38.01755085673411	40.0	36.0	41.0	34.0	41.0
26	38.02935330392656	40.0	36.0	41.0	34.0	41.0
27	38.0336689462333	40.0	36.0	41.0	34.0	41.0
28	37.93983027885856	40.0	36.0	41.0	34.0	41.0
29	37.924072006849535	40.0	36.0	41.0	34.0	41.0
30	37.80187278500905	40.0	36.0	41.0	33.0	41.0
31	37.71642046624544	40.0	36.0	41.0	33.0	41.0
32	37.63146018706174	40.0	36.0	41.0	33.0	41.0
33	37.48787566788412	40.0	36.0	41.0	33.0	41.0
34	37.385316845310996	40.0	35.0	41.0	33.0	41.0
35	37.32973804853201	40.0	35.0	41.0	33.0	41.0
36	37.25415253226978	39.0	35.0	41.0	33.0	41.0
37	37.16334630265853	39.0	35.0	41.0	32.0	41.0
38	37.10497566896791	39.0	35.0	41.0	32.0	41.0
39	37.07105528400655	39.0	35.0	41.0	32.0	41.0
40	36.90318958696854	39.0	35.0	41.0	31.0	41.0
41	36.83968396752972	39.0	35.0	41.0	31.0	41.0
42	36.85931786407136	39.0	35.0	41.0	32.0	41.0
43	36.75334727806739	39.0	35.0	41.0	31.0	41.0
44	36.57913925587142	39.0	35.0	41.0	31.0	41.0
45	36.54035049691662	39.0	35.0	41.0	31.0	41.0
46	36.41431033174739	39.0	35.0	41.0	31.0	41.0
47	36.320874833367654	39.0	35.0	40.0	31.0	41.0
48	36.30506670712807	39.0	35.0	40.0	31.0	41.0
49	36.27323152954947	38.0	35.0	40.0	31.0	41.0
50	36.140714649557275	38.0	35.0	40.0	31.0	41.0
51	34.99869728727958	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	5.0
11	7.0
12	5.0
13	9.0
14	9.0
15	17.0
16	18.0
17	47.0
18	104.0
19	172.0
20	325.0
21	459.0
22	733.0
23	1083.0
24	1653.0
25	2381.0
26	3119.0
27	3775.0
28	4438.0
29	5201.0
30	6475.0
31	8373.0
32	10700.0
33	15327.0
34	28195.0
35	39731.0
36	34625.0
37	52492.0
38	92497.0
39	149260.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32556980134173	22.890028070099383	24.77885313593948	12.005548992619406
2	32.571719645818206	25.2806468044522	24.05228191483597	18.095351634893625
3	32.43342834538144	24.611299569736314	26.667894959303773	16.287377125578473
4	30.518592376637876	25.978389274837703	24.926681767440854	18.57633658108357
5	24.804864038842947	31.80851640312564	24.08457878594111	19.3020407720903
6	24.7487238400763	35.44809199189327	24.955727275683056	14.847456892347374
7	77.42166924969382	7.61295776479641	9.738915562106449	5.22645742340331
8	76.81106330403495	7.15516587369539	9.824101269115303	6.209669553154364
9	69.21371208098061	9.976698566148977	13.5382414462062	7.271347906664211
10	38.681897495366805	27.741061461595983	19.310277558009734	14.266763485027473
11	31.27182477321744	25.65303623102017	24.461303362992986	18.6138356327694
12	28.91805481797787	23.66710379434046	28.24155458496353	19.17328680271814
13	23.91984306755248	26.55778213701243	29.52324182553187	19.99913296990322
14	18.96780066978075	30.779568435769328	28.681572359080516	21.57105853536941
15	18.18053734190248	26.55388050157691	35.400622094094444	19.864960062426167
16	19.88576878474894	25.546825044164343	33.47082985618138	21.09657631490533
17	19.901808841539413	25.648050807963674	28.40932490869089	26.040815441806025
18	22.255362039254788	26.24239993930789	29.674755334944564	21.827482686492754
19	23.668837854534026	28.095460013655725	27.423511688649494	20.81219044316076
20	26.313929922292427	26.818541438619686	27.659127117450065	19.208401521637818
21	23.73993432247017	27.66519632812754	28.03671872459873	20.558150624803563
22	19.95209658715278	28.169591086930605	26.587261160303026	25.291051165613588
23	22.058329449761025	27.63094863930464	27.07366504459786	23.237056866336474
24	22.73396265267858	25.66192328951219	28.659679849136765	22.94443420867247
25	21.206689137196673	27.483336765327465	26.73465627675601	24.575317820719853
26	19.168084622137446	31.484030389404893	27.147579360348544	22.200305628109117
27	21.962739381590783	30.03522309768178	27.565704624521782	20.43633289620566
28	18.30929131127464	30.573215272735155	28.588800138724817	22.52869327726539
29	19.34907715484074	29.32295787317517	28.029565726300277	23.298399245683814
30	20.48380279400449	30.11000444352925	26.900042267717218	22.50615049474905
31	21.93651172116312	31.1419870162243	22.857731198994244	24.063770063618335
32	21.95363556557457	31.39255871419437	23.453597633007835	23.200208087223228
33	22.33036014262645	31.105571752159445	23.691380637050365	22.87268746816374
34	19.769803509304317	29.876339832446437	25.538588258244914	24.815268400004335
35	19.818790709772514	28.422330360142627	24.25668426015238	27.50219466993248
36	22.07870465703541	31.641613109495065	24.10582102331227	22.17386121015726
37	21.015292243331995	30.9029034670366	25.842915822215478	22.238888467415926
38	19.886202299797333	29.96976232537472	23.786970705220607	26.357064669607343
39	22.614095741798437	26.491454334608587	25.954762704700386	24.939687218892587
40	21.731242345749926	25.514311415535012	28.363805828609827	24.390640410105235
41	19.16786786461325	27.526254755118185	25.645232960149126	27.660644420119436
42	21.894894276517572	28.118002796172064	25.639163749471656	24.34793917783871
43	23.099199080948097	26.452871495301782	26.871863789571798	23.576065634178324
44	20.900193997984154	28.11280061559137	26.06010686145943	24.926898524965047
45	20.25469009092978	26.89418981456394	24.75305899056021	28.09806110394607
46	20.57462419664243	28.137944488398052	26.942093227411156	24.345338087548363
47	20.165169233437016	26.508794936544234	28.883373614106578	24.44266221591217
48	20.382577030205162	25.383823385969283	27.448005288883593	26.785594294941962
49	21.335443106568835	25.150375532410667	29.105116561358635	24.40906479966186
50	19.30854349781617	24.857319359698273	28.49581116084492	27.338325981640637
51	18.743890147286738	25.19524433991915	26.03864786656407	30.022217646230043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	428.0
1	626.5
2	825.0
3	1591.5
4	2358.0
5	1650.0
6	942.0
7	953.0
8	964.0
9	1035.5
10	1107.0
11	1051.0
12	995.0
13	1013.0
14	1031.0
15	1039.0
16	1047.0
17	1040.5
18	1034.0
19	1173.0
20	1312.0
21	1784.0
22	2256.0
23	2167.0
24	2078.0
25	2748.5
26	4340.0
27	5261.0
28	6051.5
29	6842.0
30	7661.5
31	8481.0
32	9456.5
33	10432.0
34	11331.5
35	12231.0
36	12344.5
37	12458.0
38	13690.0
39	14922.0
40	17873.5
41	20825.0
42	24295.5
43	27766.0
44	30551.5
45	33337.0
46	38078.0
47	42819.0
48	47287.0
49	51755.0
50	49486.5
51	47218.0
52	39253.5
53	31289.0
54	26797.5
55	22306.0
56	20558.0
57	18810.0
58	18278.0
59	17746.0
60	17647.0
61	17548.0
62	15466.0
63	13384.0
64	11496.0
65	9608.0
66	7767.0
67	5926.0
68	4948.0
69	3970.0
70	3326.5
71	2683.0
72	2195.0
73	1707.0
74	1363.5
75	883.0
76	746.0
77	488.0
78	230.0
79	166.0
80	102.0
81	59.5
82	17.0
83	54.0
84	91.0
85	51.0
86	11.0
87	8.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.692258948192375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08689739892289	22.845966191384395
2	11.698315036032088	7.4149205875892275
3	4.536444380245302	4.313105100084186
4	2.1938003246135587	2.7810595187312557
5	1.3168191191943273	2.086648625671861
6	0.8358789557432229	1.5894535388855313
7	0.6419785828039459	1.4242026039791351
8	0.486080615592532	1.2323994191244225
9	0.3963435271786201	1.1304919526206274
>10	5.32036303210414	40.60138553558178
>50	0.40988417467267324	8.053679846955292
>100	0.06968030969435446	3.9373161024954686
>500	0.004781982037847855	1.0247067586666148
>1k	0.002732561164484488	1.5646642182302186
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	2340	0.5072126066176073	No Hit
CCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	2022	0.4382837139234196	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	1641	0.3556990972049117	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1224	0.26531120961536375	No Hit
TCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	903	0.19573204434858946	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	796	0.17253898925966465	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	712	0.15433135722723773	No Hit
ACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	654	0.14175942082389537	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCC	572	0.12398530383985955	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	552	0.11965015335594836	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	544	0.11791609316238388	No Hit
GGTCTTGGGGGATGGACGTGCCAATCAATGGAACAGAATTGAAACTTCAGA	490	0.10621118685582374	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	464	0.1005754912267392	No Hit
CGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG	462	0.10014197617834808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3351504839111727E-4	0.0	0.0	0.38019269743900985	0.0
2	4.3351504839111727E-4	0.0	0.0	1.7481494326371805	0.0
3	4.3351504839111727E-4	0.0	0.0	2.3674256792638912	0.0
4	4.3351504839111727E-4	0.0	0.0	3.106352079246551	0.0
5	4.3351504839111727E-4	0.0	0.0	4.888965958230825	0.0
6	4.3351504839111727E-4	0.0	0.0	5.98489200056357	0.0
7	4.3351504839111727E-4	0.0	0.0	6.982843641959922	0.0
8	4.3351504839111727E-4	0.0	0.0	8.655344698652852	0.0
9	4.3351504839111727E-4	0.0	0.0	9.318839480215457	0.0
10	4.3351504839111727E-4	0.0	0.0	10.67985997463937	0.0
11	4.3351504839111727E-4	0.0	0.0	12.656038322730279	0.0
12	4.3351504839111727E-4	0.0	0.0	14.147330089195721	0.0
13	4.3351504839111727E-4	0.0	0.0	14.783513422709685	0.0
14	4.3351504839111727E-4	0.0	0.0	15.057711690817067	0.0
15	4.3351504839111727E-4	0.0	0.0	15.548884240644202	0.0
16	4.3351504839111727E-4	0.0	0.0	16.533180158016236	0.0
17	4.3351504839111727E-4	0.0	0.0	17.631273775590934	0.0
18	4.3351504839111727E-4	0.0	0.0	18.917946439215772	0.0
19	4.3351504839111727E-4	0.0	0.0	19.597698035093043	0.0
20	4.3351504839111727E-4	0.0	0.0	20.289588052325268	0.0
21	4.3351504839111727E-4	0.0	0.0	21.11565097703454	0.0
22	4.3351504839111727E-4	0.0	0.0	22.014761187397717	0.0
23	4.3351504839111727E-4	0.0	0.0	22.830419750945605	0.0
24	4.3351504839111727E-4	0.0	0.0	23.501501045855054	0.0
25	4.3351504839111727E-4	0.0	0.0	24.07612524249748	0.0
26	6.502725725866759E-4	0.0	0.0	24.60523035905884	0.0
27	6.502725725866759E-4	0.0	0.0	25.159045833378492	0.0
28	6.502725725866759E-4	0.0	0.0	25.723482426383725	0.0
29	6.502725725866759E-4	0.0	0.0	26.298973653122935	0.0
30	6.502725725866759E-4	0.0	0.0	26.98544473225027	0.0
31	8.670300967822345E-4	0.0	0.0	27.60038582839307	0.0
32	8.670300967822345E-4	0.0	0.0	28.212509076721325	0.0
33	8.670300967822345E-4	0.0	0.0	28.778246214871732	0.0
34	8.670300967822345E-4	0.0	0.0	29.330544386522018	0.0
35	8.670300967822345E-4	0.0	0.0	29.895848009624036	0.0
36	8.670300967822345E-4	0.0	0.0	30.519459406734658	0.0
37	0.0010837876209777932	0.0	0.0	31.09516739099806	0.0
38	0.0010837876209777932	0.0	0.0	31.64226338206765	0.0
39	0.0010837876209777932	0.0	0.0	32.19542858381472	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTGA	30	2.1626656E-6	45.000004	15
TACGAAG	30	2.1626656E-6	45.000004	1
ACAACGA	35	1.2098099E-7	45.0	13
GTACCGT	20	7.0288905E-4	45.0	44
CGACGGT	25	3.887163E-5	45.0	28
CTATCGA	20	7.0288905E-4	45.0	1
TTAGCAC	20	7.0288905E-4	45.0	28
TAGGTCG	20	7.0288905E-4	45.0	1
CGTGAGA	55	1.8189894E-12	45.0	2
CGTTGAA	20	7.0288905E-4	45.0	16
CAACCGG	20	7.0288905E-4	45.0	2
CGACCGC	20	7.0288905E-4	45.0	25
CACGACG	25	3.887163E-5	45.0	26
TACGGGT	25	3.887163E-5	45.0	4
GCCGCAC	20	7.0288905E-4	45.0	27
CTAACGG	20	7.0288905E-4	45.0	2
ATATCAC	55	1.8189894E-12	45.0	30
TCGTGCG	20	7.0288905E-4	45.0	45
TTCCAAT	20	7.0288905E-4	45.0	15
CTACGGA	20	7.0288905E-4	45.0	11
>>END_MODULE