FastQCFastQC Report
Sat 18 Jun 2016
SRR3551382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478907
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56911.18833092855189No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC14620.3052784778673104No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC14100.29442041983099015No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC10950.22864564518789662No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT8340.17414654619790482No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6440.1344728726036579No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6260.130714314052624No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC5890.1229883881421654No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC5400.11275675653101751No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA5160.10774534512963894No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC207.029159E-445.00000410
GATAAGC253.887385E-545.00000427
ACGTTTA207.029159E-445.00000436
GCGAACG207.029159E-445.0000041
CGGCATA207.029159E-445.00000445
TGGCGTG406.8012014E-945.0000042
TTACTCA207.029159E-445.00000412
AAGCGCC253.887385E-545.00000443
TAACGAG207.029159E-445.0000041
GCGTTAG207.029159E-445.0000041
CGTTAGG302.1628402E-645.0000042
TACGCTC207.029159E-445.00000419
GTTCGCG207.029159E-445.0000042
CTACGTT207.029159E-445.00000420
ACGTGTA207.029159E-445.00000434
GCGATAT302.1628402E-645.0000049
GCGATAC302.1628402E-645.0000049
CGATGTC253.887385E-545.00000432
ATAACGG207.029159E-445.0000042
ACCCGTA207.029159E-445.00000420