##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478907
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72262046702178	33.0	31.0	34.0	30.0	34.0
2	31.87187909134759	33.0	31.0	34.0	30.0	34.0
3	31.78311237881259	33.0	31.0	34.0	30.0	34.0
4	35.43721432344902	37.0	35.0	37.0	33.0	37.0
5	33.464480577648686	37.0	35.0	37.0	30.0	37.0
6	34.39384891012242	37.0	35.0	37.0	30.0	37.0
7	35.44943590300413	37.0	35.0	37.0	32.0	37.0
8	35.369199030291895	37.0	35.0	37.0	33.0	37.0
9	37.32647257191898	39.0	37.0	39.0	34.0	39.0
10	36.827367317662926	39.0	37.0	39.0	32.0	39.0
11	36.940758852971456	39.0	37.0	39.0	33.0	39.0
12	36.94470951562621	39.0	37.0	39.0	33.0	39.0
13	37.065096146015826	39.0	37.0	39.0	33.0	39.0
14	38.10211377156734	40.0	37.0	41.0	33.0	41.0
15	38.1991639295312	40.0	37.0	41.0	33.0	41.0
16	38.2252065641137	40.0	37.0	41.0	34.0	41.0
17	38.09297629811216	40.0	37.0	41.0	34.0	41.0
18	38.033398968902105	39.0	37.0	41.0	34.0	41.0
19	37.871160789046726	39.0	37.0	41.0	34.0	41.0
20	38.026021753701656	40.0	36.0	41.0	34.0	41.0
21	37.892267183398864	40.0	36.0	41.0	34.0	41.0
22	38.02213373368942	40.0	36.0	41.0	34.0	41.0
23	38.112575092867715	40.0	36.0	41.0	34.0	41.0
24	37.941589912028846	40.0	36.0	41.0	34.0	41.0
25	37.63759560833314	39.0	35.0	41.0	33.0	41.0
26	37.72493198053067	39.0	35.0	41.0	33.0	41.0
27	37.382842597832145	40.0	35.0	41.0	33.0	41.0
28	37.455718960048614	40.0	35.0	41.0	33.0	41.0
29	37.64419396667829	40.0	35.0	41.0	33.0	41.0
30	37.33648913045748	39.0	35.0	41.0	33.0	41.0
31	37.23231023977515	39.0	35.0	41.0	33.0	41.0
32	37.25251666816313	40.0	35.0	41.0	33.0	41.0
33	37.199736065666194	40.0	35.0	41.0	33.0	41.0
34	37.04811998989366	40.0	35.0	41.0	32.0	41.0
35	36.987486088113144	40.0	35.0	41.0	32.0	41.0
36	36.76633041488222	40.0	35.0	41.0	31.0	41.0
37	36.76767931978443	40.0	35.0	41.0	31.0	41.0
38	36.574725364214764	39.0	35.0	41.0	31.0	41.0
39	36.54332678369704	39.0	35.0	41.0	31.0	41.0
40	36.457211943028604	39.0	35.0	41.0	30.0	41.0
41	36.289502972393386	39.0	35.0	41.0	30.0	41.0
42	36.428709540683265	39.0	35.0	41.0	30.0	41.0
43	36.35404368697889	39.0	35.0	41.0	30.0	41.0
44	36.39167938660324	39.0	35.0	41.0	30.0	41.0
45	36.366532541808745	39.0	35.0	41.0	30.0	41.0
46	36.26358562309593	39.0	35.0	41.0	30.0	41.0
47	36.219226279841386	39.0	35.0	41.0	30.0	41.0
48	36.17635156721451	39.0	35.0	41.0	30.0	41.0
49	36.142271881597054	39.0	35.0	41.0	30.0	41.0
50	36.083559020853734	39.0	35.0	41.0	30.0	41.0
51	35.16839177543866	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	14.0
9	31.0
10	39.0
11	39.0
12	34.0
13	25.0
14	37.0
15	46.0
16	72.0
17	126.0
18	194.0
19	329.0
20	465.0
21	732.0
22	1103.0
23	1726.0
24	2757.0
25	3982.0
26	5565.0
27	6307.0
28	6267.0
29	6354.0
30	7044.0
31	8783.0
32	11407.0
33	16277.0
34	29831.0
35	36878.0
36	38808.0
37	55321.0
38	96479.0
39	141792.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.759911632112285	20.796730889295834	22.98337673076401	16.459980747827867
2	36.69877450110356	22.5287999548973	23.85515350579549	16.91727203820366
3	32.213143679252966	21.879613369610386	29.653147688382088	16.254095262754564
4	28.829188130472094	24.60415070149319	28.676757700346833	17.88990346768788
5	23.74218794045608	32.42717270785351	26.244552700211106	17.586086651479306
6	25.558198147030637	32.82912966400575	27.73941495948065	13.873257229482968
7	74.80742607646161	6.359063450732607	13.779293265707121	5.054217207098664
8	74.96716481488055	6.345073260570423	13.365225398668217	5.322536525880808
9	69.27733359504037	8.830524506845796	15.684673642272925	6.2074682558409044
10	37.11096308886694	25.42497812727732	23.12870766140399	14.335351122451751
11	28.60137772051776	25.922151900055752	27.608700645428026	17.867769733998458
12	26.77910324969148	22.69417653114279	31.780909445884063	18.74581077328166
13	23.263180534007645	24.50580175274114	32.75166159609277	19.479356117158446
14	19.87149905931632	27.41450845362461	31.7800742106505	20.933918276408573
15	18.71344540798109	26.913158504678364	35.03644757750461	19.33694850983594
16	21.065467825694757	25.06227722710255	34.3555220533423	19.516732893860393
17	20.845800959267667	26.35814469197569	31.2641076451169	21.531946703639747
18	21.432971328462518	25.833408156489675	32.57500934419418	20.158611170853632
19	22.395997552760765	26.570920867725885	30.976160298346024	20.056921281167327
20	24.5043400910824	25.97665099904574	31.623885221974202	17.89512368789765
21	23.42605140455245	25.65842637505821	31.777986122566592	19.13753609782275
22	21.345271628938395	24.9931615115252	31.203553090683577	22.458013768852826
23	21.36343799526839	25.76178673521164	31.69801234895293	21.17676292056704
24	20.734297055586993	25.200926275874018	32.95942636044159	21.1053503080974
25	21.251516473970938	26.15935870638767	30.48003056960955	22.109094250031845
26	19.28850486628928	27.56109223711493	31.919558494655536	21.230844401940253
27	20.21728644601144	28.602421764559715	31.922481817973008	19.257809971455835
28	18.594424387198348	27.020277423382826	33.40230984303842	20.982988346380406
29	19.584178138970614	26.47695690395004	32.44930644154293	21.48955851553642
30	20.422127887042787	26.139104251973766	32.62220013489049	20.81656772609296
31	20.6142319907623	27.133242988722234	30.79679353193835	21.455731488577115
32	21.22771226981439	27.709555299880773	29.77363037082356	21.289102059481277
33	20.561612171047823	27.641483628345377	29.354968710000062	22.441935490606735
34	20.042304664579973	26.028853201143438	31.258678616098738	22.67016351817785
35	20.22125381337086	25.901271019216676	30.428245985128637	23.449229182283826
36	20.22605641596385	28.926910652798977	29.690942082700815	21.156090848536355
37	20.44467923834899	28.621423366123278	30.77946240084192	20.154434994685815
38	19.851662222519195	28.483609552585364	28.934845387517825	22.729882837377613
39	21.070061619479354	25.442726875990534	30.560004343223213	22.927207161306892
40	20.988417375398562	24.47448043148252	33.03522395788744	21.501878235231477
41	17.929368332473736	26.733165311845514	30.93961875687764	24.397847598803107
42	19.614246607378885	27.541464209126197	30.641439778495617	22.2028494049993
43	21.282420177612774	26.32473528263316	30.71264358215687	21.680200957597194
44	21.48642638341056	25.33017892826791	29.8181066470108	23.365288041310734
45	21.157552510195092	25.080861211049328	29.884090230462277	23.877496048293303
46	21.093865823635905	25.75614889738509	30.66712326192768	22.48286201705133
47	19.827649209554256	26.12532287061998	32.0878583942185	21.95916952560727
48	19.618422783546702	25.91359073891173	31.922064200356225	22.54592227718534
49	20.284314073504877	25.342916265579746	32.30084337877709	22.071926282138286
50	19.986761521548026	23.72903298552746	32.02584217812644	24.25836331479807
51	18.968818580643006	24.70417012071237	30.003737677670195	26.323273620974426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	1031.5
2	1642.0
3	5526.0
4	9410.0
5	6174.5
6	2939.0
7	2891.0
8	2843.0
9	2802.0
10	2761.0
11	2740.5
12	2720.0
13	2753.5
14	2787.0
15	2658.0
16	2529.0
17	2265.0
18	2001.0
19	2097.0
20	2193.0
21	2591.5
22	2990.0
23	3249.5
24	3509.0
25	3764.0
26	4691.5
27	5364.0
28	7169.0
29	8974.0
30	9255.5
31	9537.0
32	9929.0
33	10321.0
34	11767.5
35	13214.0
36	14530.0
37	15846.0
38	16730.0
39	17614.0
40	20183.0
41	22752.0
42	25557.0
43	28362.0
44	32673.5
45	36985.0
46	41383.0
47	45781.0
48	44708.5
49	43636.0
50	39057.5
51	34479.0
52	30939.5
53	27400.0
54	24488.0
55	21576.0
56	20498.5
57	19421.0
58	19021.5
59	18622.0
60	16930.5
61	15239.0
62	14136.0
63	13033.0
64	10894.0
65	8755.0
66	7289.0
67	5823.0
68	5361.5
69	4900.0
70	4066.0
71	3232.0
72	2529.0
73	1826.0
74	1545.0
75	1125.0
76	986.0
77	760.5
78	535.0
79	429.0
80	323.0
81	264.5
82	206.0
83	137.0
84	68.0
85	61.5
86	55.0
87	28.5
88	2.0
89	6.0
90	10.0
91	6.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	478907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.6630100018299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29762008798788	21.742280377803127
2	10.978694216953235	6.5132223272903165
3	4.214041155113486	3.750034347967625
4	2.0911307939382633	2.4811693462290076
5	1.2455361596608685	1.8473175780830575
6	0.7984653594844024	1.4210931566700318
7	0.56954247487373	1.1826040890052483
8	0.41799511399631445	0.9919194597750964
9	0.337320689721647	0.9005352293733637
>10	5.263857444228072	39.69488939680691
>50	0.7130639782596339	13.083971523852217
>100	0.06560188599988631	3.555164948315628
>500	0.0042783838695578036	0.7929743807255644
>1k	0.0021391919347789018	0.8390849208691156
>5k	7.130639782596339E-4	1.2037389172337123
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5691	1.18833092855189	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1462	0.3052784778673104	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1410	0.29442041983099015	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC	1095	0.22864564518789662	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	834	0.17414654619790482	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	644	0.1344728726036579	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	626	0.130714314052624	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	589	0.1229883881421654	No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	540	0.11275675653101751	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	516	0.10774534512963894	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1795755752160649	0.0
2	0.0	0.0	0.0	1.001447045042148	0.0
3	0.0	0.0	0.0	1.3121545519276185	0.0
4	0.0	0.0	0.0	1.7189141106728447	0.0
5	0.0	0.0	0.0	2.809940134514634	0.0
6	0.0	0.0	0.0	3.39209909230811	0.0
7	0.0	0.0	0.0	4.008293885869281	0.0
8	2.0880880839077316E-4	0.0	0.0	4.925590981129948	0.0
9	2.0880880839077316E-4	0.0	0.0	5.27451049995093	0.0
10	2.0880880839077316E-4	0.0	0.0	6.146078466174017	0.0
11	2.0880880839077316E-4	0.0	0.0	7.7232113959495265	0.0
12	2.0880880839077316E-4	0.0	0.0	8.805258641030512	0.0
13	2.0880880839077316E-4	0.0	0.0	9.269649430891592	0.0
14	2.0880880839077316E-4	0.0	0.0	9.464676857928575	0.0
15	2.0880880839077316E-4	0.0	0.0	9.842620801115874	0.0
16	2.0880880839077316E-4	0.0	0.0	10.539415794715884	0.0
17	2.0880880839077316E-4	0.0	0.0	11.329339516858179	0.0
18	2.0880880839077316E-4	0.0	0.0	12.171047823481386	0.0
19	2.0880880839077316E-4	0.0	0.0	12.699960535135213	0.0
20	2.0880880839077316E-4	0.0	0.0	13.223861835387664	0.0
21	2.0880880839077316E-4	0.0	0.0	13.85759656885366	0.0
22	2.0880880839077316E-4	0.0	0.0	14.53288425518942	0.0
23	2.0880880839077316E-4	0.0	0.0	15.187708678302885	0.0
24	2.0880880839077316E-4	0.0	0.0	15.716203772339933	0.0
25	2.0880880839077316E-4	0.0	0.0	16.192287855470894	0.0
26	2.0880880839077316E-4	0.0	0.0	16.618257824588074	0.0
27	2.0880880839077316E-4	0.0	0.0	17.046524690597547	0.0
28	2.0880880839077316E-4	0.0	0.0	17.50005742242231	0.0
29	2.0880880839077316E-4	0.0	0.0	17.963404168241432	0.0
30	2.0880880839077316E-4	0.0	0.0	18.440114677797567	0.0
31	2.0880880839077316E-4	0.0	0.0	18.894691453664283	0.0
32	2.0880880839077316E-4	0.0	0.0	19.357829390675015	0.0
33	2.0880880839077316E-4	0.0	0.0	19.81512068105081	0.0
34	2.0880880839077316E-4	0.0	0.0	20.247146105611318	0.0
35	2.0880880839077316E-4	0.0	0.0	20.71487783640665	0.0
36	2.0880880839077316E-4	0.0	0.0	21.163816774446815	0.0
37	2.0880880839077316E-4	0.0	0.0	21.606909065852033	0.0
38	2.0880880839077316E-4	0.0	0.0	22.03183499092726	0.0
39	2.0880880839077316E-4	0.0	0.0	22.481191546584203	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAC	20	7.029159E-4	45.000004	10
GATAAGC	25	3.887385E-5	45.000004	27
ACGTTTA	20	7.029159E-4	45.000004	36
GCGAACG	20	7.029159E-4	45.000004	1
CGGCATA	20	7.029159E-4	45.000004	45
TGGCGTG	40	6.8012014E-9	45.000004	2
TTACTCA	20	7.029159E-4	45.000004	12
AAGCGCC	25	3.887385E-5	45.000004	43
TAACGAG	20	7.029159E-4	45.000004	1
GCGTTAG	20	7.029159E-4	45.000004	1
CGTTAGG	30	2.1628402E-6	45.000004	2
TACGCTC	20	7.029159E-4	45.000004	19
GTTCGCG	20	7.029159E-4	45.000004	2
CTACGTT	20	7.029159E-4	45.000004	20
ACGTGTA	20	7.029159E-4	45.000004	34
GCGATAT	30	2.1628402E-6	45.000004	9
GCGATAC	30	2.1628402E-6	45.000004	9
CGATGTC	25	3.887385E-5	45.000004	32
ATAACGG	20	7.029159E-4	45.000004	2
ACCCGTA	20	7.029159E-4	45.000004	20
>>END_MODULE