SRR3551381_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657092
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55794	8.491048437661696	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	37471	5.702550023436596	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27545	4.191954855636654	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	21735	3.3077559915506503	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	17702	2.6939911001807966	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	16625	2.530087111089467	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	8343	1.2696852191169579	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	6640	1.0105129875268608	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	6060	0.922245286809153	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	5201	0.7915177783324101	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT	4977	0.7574281835724678	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	4518	0.6875749514527646	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	3602	0.5481728585951435	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG	3601	0.5480206729042508	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	3455	0.5258015620339314	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	2837	0.43175080506230484	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCC	2694	0.4099882512646631	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2551	0.38822569746702135	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	2203	0.33526507703639674	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1984	0.3019364107309174	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	1945	0.29600116878610605	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGT	1561	0.23756186348334782	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	1487	0.22630012235729546	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGT	1418	0.2157993096857061	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1417	0.21564712399481353	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	1386	0.21092936757714292	No Hit
GAATATGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	1363	0.20742909668661313	No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	1340	0.20392882579608335	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGCCACTTTCG	1261	0.191906156215568	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	1130	0.17196983070863744	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATG	1110	0.16892611689078546	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTAT	1051	0.15994716112812207	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	1032	0.1570556330011627	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCCACTT	943	0.14351110651172136	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	884	0.13453215074905797	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	836	0.12722723758621318	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	814	0.123879152386576	No Hit
GAACAGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	674	0.10257315566161208	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	30	2.1640772E-6	45.000004	45
CGACGGA	30	2.1640772E-6	45.000004	41
GGCCGAT	30	2.1640772E-6	45.000004	8
CCGTCTA	30	2.1640772E-6	45.000004	41
CTACGGC	30	2.1640772E-6	45.000004	28
AGGCACG	30	2.1640772E-6	45.000004	1
CGCCGAA	30	2.1640772E-6	45.000004	41
TCGAATA	65	0.0	45.000004	44
GCCCCTA	45	3.8380676E-10	45.0	37
TATCAGC	20	7.0310786E-4	45.0	39
GATACGT	20	7.0310786E-4	45.0	27
GTCGACG	20	7.0310786E-4	45.0	1
CTATCTA	25	3.8889757E-5	45.0	18
GCGCGAC	55	1.8189894E-12	45.0	9
CGGCAAC	20	7.0310786E-4	45.0	31
GGCAACC	20	7.0310786E-4	45.0	32
ACCGACC	40	6.8066583E-9	45.0	39
GCGCCCC	100	0.0	45.0	33
CCTCGAA	20	7.0310786E-4	45.0	42
CTAACGT	25	3.8889757E-5	45.0	22

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content