FastQCFastQC Report
Sat 18 Jun 2016
SRR3551376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597172
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG75561.2652971003329023No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC66011.1053766753967031No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC64331.0772440770833194No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC37300.6246106649340558TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.5171039499507679No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC21900.36672851372803816TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT16160.270608802823977No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGTCCCT15220.25486794424386944No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT13750.23025192071965866Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT10990.18403408063338536No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTA10180.1704701493037182No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC9510.15925060116683298No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8760.14669140549121526No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8760.14669140549121526No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTC8520.14267246287501759No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7900.13229019444984025No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC6280.10516233179050592TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCC207.03056E-445.00000430
CGGAATA207.03056E-445.00000429
TCGCATA207.03056E-445.00000430
AGCCGAT207.03056E-445.00000425
CGCATCT207.03056E-445.00000439
TAGAACG207.03056E-445.0000041
TCGCTAG207.03056E-445.0000041
ATCATAC207.03056E-445.00000431
CCGTTTG207.03056E-445.0000041
TCGCGAT207.03056E-445.00000426
ACGGTGC207.03056E-445.00000414
CCCGATT207.03056E-445.00000414
TTGGCCG502.1827873E-1145.01
CGCGCGA253.888546E-545.041
TTGCGTC253.888546E-545.016
ATGCGAG453.8380676E-1045.01
TTACGAG253.888546E-545.01
ACGTATG253.888546E-545.01
CCGCGAC253.888546E-545.012
CCGTTGA502.1827873E-1145.041
Produced by FastQC (version 0.11.5)