SRR3551375_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	921421
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG	19332	2.098063751531602	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC	16309	1.7699835362988254	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	15503	1.6825099493065603	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7798	0.8463015277489877	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	6018	0.6531216458057717	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTCTTCT	4106	0.44561606475216	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	3789	0.4112126812824974	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	3455	0.3749643214122535	TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTA	3068	0.3329639762931385	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC	2876	0.3121265957689265	No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	1832	0.1988233391685234	TruSeq Adapter, Index 23 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1784	0.19361399403747037	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	1482	0.16083853092126182	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	1291	0.14010967842061336	TruSeq Adapter, Index 23 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1196	0.12979951618207095	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1112	0.12068316220272816	Illumina PCR Primer Index 7 (95% over 23bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	932	0.10114811796127937	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTG	20	7.032556E-4	45.000004	1
TCCGATC	20	7.032556E-4	45.000004	14
CGACAAT	20	7.032556E-4	45.000004	21
ACGGGTC	40	6.8121153E-9	45.000004	5
CGCTCGA	40	6.8121153E-9	45.000004	41
TCGCTTC	20	7.032556E-4	45.000004	12
GCGATTC	20	7.032556E-4	45.000004	9
CGGCTAC	20	7.032556E-4	45.000004	31
CCGCGTA	20	7.032556E-4	45.000004	40
ACCCGGT	20	7.032556E-4	45.000004	17
CCTATCG	40	6.8121153E-9	45.000004	40
TCGACAC	40	6.8121153E-9	45.000004	34
GACTCGC	20	7.032556E-4	45.000004	9
TACGTTC	20	7.032556E-4	45.000004	29
TACCGGT	20	7.032556E-4	45.000004	25
CTCCGAC	25	3.8902006E-5	45.0	12
TATTGCG	25	3.8902006E-5	45.0	1
AGTGCCG	25	3.8902006E-5	45.0	2
CGGCGAA	435	0.0	43.448277	31
CGATGAA	2890	0.0	42.82007	19

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content