##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	921421
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5821877296046	31.0	31.0	34.0	30.0	34.0
2	31.720335221359182	31.0	31.0	34.0	30.0	34.0
3	31.67827952694805	31.0	31.0	34.0	30.0	34.0
4	35.420077250247175	37.0	35.0	37.0	33.0	37.0
5	33.401441903321064	37.0	35.0	37.0	30.0	37.0
6	34.28684282211931	37.0	35.0	37.0	30.0	37.0
7	35.333098551042355	37.0	35.0	37.0	32.0	37.0
8	35.07046616041961	37.0	35.0	37.0	32.0	37.0
9	36.76870399090101	39.0	37.0	39.0	32.0	39.0
10	36.529249930270744	38.0	35.0	39.0	32.0	39.0
11	36.71145545847121	39.0	35.0	39.0	32.0	39.0
12	36.73810234409678	39.0	35.0	39.0	33.0	39.0
13	36.77230603600309	39.0	35.0	39.0	32.0	39.0
14	37.82790277191425	40.0	37.0	41.0	33.0	41.0
15	37.81820036660766	40.0	37.0	41.0	33.0	41.0
16	38.02777123594969	40.0	37.0	41.0	33.0	41.0
17	37.73378509931942	40.0	36.0	41.0	32.0	41.0
18	37.74860460093703	39.0	37.0	41.0	33.0	41.0
19	37.670668456655534	39.0	37.0	41.0	33.0	41.0
20	37.523790970685496	39.0	36.0	41.0	32.0	41.0
21	37.66330265969627	39.0	36.0	41.0	33.0	41.0
22	37.75244432241071	40.0	36.0	41.0	33.0	41.0
23	37.675519659308826	40.0	36.0	41.0	33.0	41.0
24	37.60783507213315	40.0	36.0	41.0	33.0	41.0
25	37.487092219517464	39.0	36.0	41.0	33.0	41.0
26	37.471170073180446	39.0	36.0	41.0	33.0	41.0
27	36.97589701124676	39.0	35.0	41.0	31.0	41.0
28	36.99227714584321	39.0	35.0	41.0	31.0	41.0
29	37.20420307329657	39.0	35.0	41.0	31.0	41.0
30	37.03671828621228	39.0	35.0	41.0	31.0	41.0
31	37.02565276892973	39.0	35.0	41.0	31.0	41.0
32	36.85146854695085	39.0	35.0	41.0	31.0	41.0
33	36.79665755393029	39.0	35.0	41.0	31.0	41.0
34	36.626597396846826	40.0	35.0	41.0	30.0	41.0
35	36.536573401300814	40.0	35.0	41.0	30.0	41.0
36	36.47171271329827	39.0	35.0	41.0	30.0	41.0
37	36.25689125817623	39.0	35.0	41.0	30.0	41.0
38	36.251118652602884	39.0	35.0	41.0	29.0	41.0
39	36.092142462565974	39.0	35.0	41.0	29.0	41.0
40	36.18482430940905	39.0	35.0	41.0	29.0	41.0
41	36.05123933576508	39.0	35.0	41.0	28.0	41.0
42	36.113168681851185	39.0	35.0	41.0	29.0	41.0
43	36.07031205062615	39.0	35.0	41.0	29.0	41.0
44	36.29425854196941	39.0	35.0	41.0	30.0	41.0
45	36.30673817939899	39.0	35.0	41.0	30.0	41.0
46	36.13260388031095	39.0	35.0	41.0	29.0	41.0
47	35.975920887411945	39.0	35.0	41.0	29.0	41.0
48	36.035884790991304	39.0	35.0	41.0	29.0	41.0
49	36.054495176471995	39.0	35.0	41.0	29.0	41.0
50	35.892469348972945	39.0	35.0	41.0	28.0	41.0
51	35.15494871508246	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	38.0
10	24.0
11	33.0
12	28.0
13	32.0
14	49.0
15	57.0
16	88.0
17	156.0
18	287.0
19	650.0
20	1690.0
21	3681.0
22	6008.0
23	7453.0
24	8394.0
25	9388.0
26	10327.0
27	10547.0
28	10363.0
29	11528.0
30	13986.0
31	18546.0
32	24956.0
33	34305.0
34	55044.0
35	68594.0
36	76558.0
37	110037.0
38	184657.0
39	253818.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.46049851262344	19.071521052808652	20.981831323575218	12.486149110992695
2	30.01548694896253	33.439437564370685	21.24056213175085	15.304513354915938
3	28.04526920918885	32.72944723421759	25.599590198183027	13.625693358410542
4	25.7398084046272	21.754008211230264	36.41690389083817	16.089279493304364
5	30.437552432601382	29.994541040414752	22.66716300149443	16.900743525489435
6	22.5488674558101	40.122267671346755	24.065329529064346	13.263535343778793
7	69.49667958511907	5.337408198858068	20.61674305230725	4.549169163715608
8	68.61608320192398	14.588011343349022	11.55411044462846	5.241795010098533
9	63.13954207685738	8.143725832165753	12.4670481788455	16.24968391213137
10	39.518743332309555	27.83266281102775	19.176467651594656	13.472126205068042
11	30.260434698145584	22.08393340286362	29.12772771621224	18.527904182778556
12	24.70217197133558	19.684921441990145	31.22210151494268	24.3908050717316
13	25.493992431255634	22.09619706952631	34.801355732070355	17.6084547671477
14	18.847085100079116	30.75749304606689	28.50217218839163	21.893249665462367
15	16.43483272033088	24.94397240783529	36.516858200540256	22.104336671293577
16	18.8026971384416	25.945360481256667	28.626545303395517	26.625397076906214
17	19.25547605274896	29.603080459420827	30.22277547396901	20.9186680138612
18	18.537997288969972	26.403240212671513	31.060828871927164	23.99793362643135
19	19.32894952470152	27.18876604722488	28.677553474470415	24.804730953603187
20	23.800955263663408	26.36992210943749	33.62979571770124	16.199326909197858
21	21.31761702848101	31.867517671075436	29.411854081901755	17.403011218541796
22	19.020404353710195	23.502177614792803	33.50835285933357	23.969065172163432
23	21.66544934400236	27.737483734362467	30.75510542954849	19.84196149208668
24	20.77975214369979	27.310100377568997	28.865741067329704	23.044406411401518
25	17.956829722786868	34.70498284714588	26.596203038567605	20.741984391499653
26	17.85687541308479	27.286983908549946	32.08761250286243	22.768528175502837
27	23.195151836131366	29.028316046627978	27.357744179913418	20.418787937327238
28	17.502639944173186	29.733314087697156	32.025208889313355	20.738837078816307
29	19.951357739838794	26.334867557826446	31.498630918982744	22.21514378335202
30	18.522369253576812	33.25895546118441	28.688623332873895	19.53005195236488
31	23.139151375972546	25.63529591793545	26.62789322144818	24.597659484643827
32	24.065221001040786	28.233131217977448	28.39993879019471	19.301708990787056
33	21.532393987113384	25.08668675882143	25.85246049308622	27.528458760978967
34	19.871806698566672	26.636792519380393	30.140511232107798	23.35088954994514
35	20.517222854699426	26.91191105911413	29.86116009945508	22.70970598673136
36	21.276593435573968	30.193147323536145	26.10077261099975	22.429486629890135
37	18.710556846436102	27.870756147298575	33.37334399802045	20.045343008244874
38	20.648867347282078	24.94701119249507	26.775816917565372	27.628304542657485
39	20.14909579877168	25.270858814808868	32.25094717832565	22.329098208093804
40	18.942481232791526	23.604302484966155	32.61603544959362	24.837180832648702
41	17.56667147807571	25.42420891210424	30.069968016791453	26.939151593028594
42	19.012915920084307	24.809506186639982	33.69458694776872	22.482990945506995
43	21.392392836716333	25.19792798297412	26.839739923444334	26.56993925686521
44	20.891427479946735	24.635535764867527	30.580592367658216	23.892444387527526
45	20.331422878358534	23.267648555871855	29.58278571901444	26.818142846755176
46	24.025608272440067	26.8219413275799	27.848507902468032	21.303942497511997
47	17.45760081439429	25.302983109783696	35.41236850473345	21.827047571088567
48	20.62184386941474	25.245029145200725	29.885036264639076	24.24809072074546
49	19.41631458367022	22.60237177142696	35.200304746690165	22.78100889821265
50	20.490199376832088	22.547999231621592	30.939494541583056	26.02230684996326
51	19.82806990507054	22.45726980392242	28.926625288548884	28.788035002458162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	313.0
1	836.5
2	1360.0
3	6720.5
4	12081.0
5	7768.5
6	3456.0
7	3269.0
8	3082.0
9	3069.0
10	3056.0
11	3020.5
12	2985.0
13	2987.5
14	2990.0
15	3042.5
16	3095.0
17	2901.0
18	2707.0
19	2758.5
20	2810.0
21	3507.5
22	4205.0
23	4658.5
24	5112.0
25	5923.0
26	8251.0
27	9768.0
28	11907.0
29	14046.0
30	15612.0
31	17178.0
32	18964.0
33	20750.0
34	24056.5
35	27363.0
36	28559.5
37	29756.0
38	32035.0
39	34314.0
40	39819.5
41	45325.0
42	51835.0
43	58345.0
44	97413.0
45	136481.0
46	113386.0
47	90291.0
48	87868.5
49	85446.0
50	72952.5
51	60459.0
52	55252.5
53	50046.0
54	45202.0
55	40358.0
56	37065.5
57	33773.0
58	31701.0
59	29629.0
60	27205.5
61	24782.0
62	22175.5
63	19569.0
64	16840.5
65	14112.0
66	11748.0
67	9384.0
68	7605.5
69	5827.0
70	5011.5
71	4196.0
72	3496.0
73	2796.0
74	2254.5
75	1322.5
76	932.0
77	727.5
78	523.0
79	321.5
80	120.0
81	119.0
82	118.0
83	62.5
84	7.0
85	13.5
86	20.0
87	11.5
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	921421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.456831327137238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70922114326618	21.25987704655721
2	10.403083052269583	5.920775594012711
3	3.7201027026612357	3.1758700538777456
4	1.8626518083256223	2.1202067332283763
5	1.0970932265683666	1.5609898449300388
6	0.72127272169315	1.2315081709252305
7	0.5805731563299954	1.1564890667922454
8	0.48709579004548786	1.1088962189986482
9	0.3798915925573107	0.9729459874800842
>10	5.457322510345366	38.19164039216352
>50	0.5093194029267384	8.76698586508827
>100	0.05960120600908913	3.1568770045369248
>500	0.006579353910094254	1.3601531888791174
>1k	0.004257229000649223	2.862489192887218
>5k	7.74041636481677E-4	1.5216094297614482
>10k+	0.0011610624547225155	5.632686209881261
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG	19332	2.098063751531602	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC	16309	1.7699835362988254	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	15503	1.6825099493065603	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7798	0.8463015277489877	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	6018	0.6531216458057717	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTCTTCT	4106	0.44561606475216	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	3789	0.4112126812824974	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	3455	0.3749643214122535	TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTA	3068	0.3329639762931385	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC	2876	0.3121265957689265	No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	1832	0.1988233391685234	TruSeq Adapter, Index 23 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1784	0.19361399403747037	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	1482	0.16083853092126182	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	1291	0.14010967842061336	TruSeq Adapter, Index 23 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1196	0.12979951618207095	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1112	0.12068316220272816	Illumina PCR Primer Index 7 (95% over 23bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	932	0.10114811796127937	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2085908612892478	0.0
2	0.0	0.0	0.0	1.291266424359766	0.0
3	1.0852802356360448E-4	0.0	0.0	1.6071915009534188	0.0
4	1.0852802356360448E-4	0.0	0.0	2.3296625538163336	0.0
5	1.0852802356360448E-4	0.0	0.0	5.053064777121424	0.0
6	1.0852802356360448E-4	0.0	0.0	5.857691543821988	0.0
7	1.0852802356360448E-4	0.0	0.0	6.959359511016137	0.0
8	1.0852802356360448E-4	0.0	0.0	8.195602227429156	0.0
9	1.0852802356360448E-4	0.0	0.0	8.59585357833173	0.0
10	1.0852802356360448E-4	0.0	0.0	11.701491500627835	0.0
11	1.0852802356360448E-4	0.0	0.0	13.078495063602848	0.0
12	1.0852802356360448E-4	0.0	0.0	16.00734083551384	0.0
13	1.0852802356360448E-4	0.0	0.0	16.540973127376084	0.0
14	1.0852802356360448E-4	0.0	0.0	16.790696109595938	0.0
15	1.0852802356360448E-4	0.0	0.0	17.470515649198358	0.0
16	1.0852802356360448E-4	0.0	0.0	18.142846755174887	0.0
17	1.0852802356360448E-4	0.0	0.0	18.914806586782806	0.0
18	1.0852802356360448E-4	0.0	0.0	19.685572610131526	0.0
19	1.0852802356360448E-4	0.0	0.0	20.693580892990283	0.0
20	1.0852802356360448E-4	0.0	0.0	21.27822135592742	0.0
21	1.0852802356360448E-4	0.0	0.0	21.815543600590825	0.0
22	1.0852802356360448E-4	0.0	0.0	22.4910220192507	0.0
23	1.0852802356360448E-4	0.0	0.0	23.040933514647485	0.0
24	1.0852802356360448E-4	0.0	0.0	23.498813246062333	0.0
25	1.0852802356360448E-4	0.0	0.0	23.908289478967813	0.0
26	1.0852802356360448E-4	0.0	0.0	24.2938895466893	0.0
27	1.0852802356360448E-4	0.0	0.0	24.72550549640175	0.0
28	1.0852802356360448E-4	0.0	0.0	25.094500776518007	0.0
29	1.0852802356360448E-4	0.0	0.0	25.51276777933214	0.0
30	1.0852802356360448E-4	0.0	0.0	25.956104755589465	0.0
31	1.0852802356360448E-4	0.0	0.0	26.347782392630513	0.0
32	1.0852802356360448E-4	0.0	0.0	26.723831994278402	0.0
33	1.0852802356360448E-4	0.0	0.0	27.1043312448924	0.0
34	1.0852802356360448E-4	0.0	0.0	27.495574769839195	0.0
35	2.1705604712720896E-4	0.0	0.0	27.890725303634277	0.0
36	2.1705604712720896E-4	0.0	0.0	28.265906681093657	0.0
37	2.1705604712720896E-4	0.0	0.0	28.645537707519146	0.0
38	2.1705604712720896E-4	0.0	0.0	29.013990347517584	0.0
39	2.1705604712720896E-4	0.0	0.0	29.381900347398204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTG	20	7.032556E-4	45.000004	1
TCCGATC	20	7.032556E-4	45.000004	14
CGACAAT	20	7.032556E-4	45.000004	21
ACGGGTC	40	6.8121153E-9	45.000004	5
CGCTCGA	40	6.8121153E-9	45.000004	41
TCGCTTC	20	7.032556E-4	45.000004	12
GCGATTC	20	7.032556E-4	45.000004	9
CGGCTAC	20	7.032556E-4	45.000004	31
CCGCGTA	20	7.032556E-4	45.000004	40
ACCCGGT	20	7.032556E-4	45.000004	17
CCTATCG	40	6.8121153E-9	45.000004	40
TCGACAC	40	6.8121153E-9	45.000004	34
GACTCGC	20	7.032556E-4	45.000004	9
TACGTTC	20	7.032556E-4	45.000004	29
TACCGGT	20	7.032556E-4	45.000004	25
CTCCGAC	25	3.8902006E-5	45.0	12
TATTGCG	25	3.8902006E-5	45.0	1
AGTGCCG	25	3.8902006E-5	45.0	2
CGGCGAA	435	0.0	43.448277	31
CGATGAA	2890	0.0	42.82007	19
>>END_MODULE