##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1184464
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58102061354334	31.0	31.0	34.0	30.0	34.0
2	31.74714892136865	31.0	31.0	34.0	30.0	34.0
3	31.70573018681868	31.0	31.0	34.0	30.0	34.0
4	35.41056292128761	37.0	35.0	37.0	33.0	37.0
5	33.40576497048454	37.0	35.0	37.0	30.0	37.0
6	34.27166380742682	37.0	35.0	37.0	30.0	37.0
7	35.33637577841116	37.0	35.0	37.0	32.0	37.0
8	35.12609247727242	37.0	35.0	37.0	32.0	37.0
9	36.90812637614989	39.0	37.0	39.0	32.0	39.0
10	36.527804981831444	38.0	35.0	39.0	32.0	39.0
11	36.66290153183212	39.0	35.0	39.0	32.0	39.0
12	36.634177146793824	39.0	35.0	39.0	32.0	39.0
13	36.699095118129385	39.0	35.0	39.0	32.0	39.0
14	37.76378429399289	40.0	37.0	41.0	33.0	41.0
15	37.84165495954288	40.0	37.0	41.0	33.0	41.0
16	37.95480571802942	40.0	37.0	41.0	33.0	41.0
17	37.68846668197598	40.0	36.0	41.0	33.0	41.0
18	37.70057764524713	39.0	36.0	41.0	33.0	41.0
19	37.58500722689757	39.0	36.0	41.0	33.0	41.0
20	37.50447037647408	39.0	36.0	41.0	32.0	41.0
21	37.533550196544596	39.0	35.0	41.0	33.0	41.0
22	37.60500108065758	39.0	36.0	41.0	33.0	41.0
23	37.54330904105148	40.0	35.0	41.0	33.0	41.0
24	37.49403274392468	39.0	35.0	41.0	33.0	41.0
25	37.33741675559578	39.0	35.0	41.0	32.0	41.0
26	37.3268524834862	39.0	35.0	41.0	32.0	41.0
27	36.8450928014697	39.0	35.0	41.0	31.0	41.0
28	36.87091629631631	39.0	35.0	41.0	31.0	41.0
29	37.10120442664361	39.0	35.0	41.0	31.0	41.0
30	36.89729531670021	39.0	35.0	41.0	31.0	41.0
31	36.87511397560416	39.0	35.0	41.0	31.0	41.0
32	36.71405547150441	39.0	35.0	41.0	31.0	41.0
33	36.64688331599778	39.0	35.0	41.0	30.0	41.0
34	36.525672371638144	39.0	35.0	41.0	30.0	41.0
35	36.4073994650745	40.0	35.0	41.0	30.0	41.0
36	36.23198510043361	39.0	35.0	41.0	29.0	41.0
37	36.14942539410231	39.0	35.0	41.0	29.0	41.0
38	36.01704906185414	39.0	35.0	41.0	28.0	41.0
39	35.927678679976765	39.0	35.0	41.0	28.0	41.0
40	35.86464932661524	39.0	35.0	41.0	27.0	41.0
41	35.70202555755177	39.0	35.0	41.0	26.0	41.0
42	35.82704075429899	39.0	35.0	41.0	28.0	41.0
43	35.81529704575234	39.0	35.0	41.0	28.0	41.0
44	35.95149282713531	39.0	35.0	41.0	28.0	41.0
45	35.95203991003525	39.0	35.0	41.0	28.0	41.0
46	35.79703139985681	39.0	35.0	41.0	28.0	41.0
47	35.71455443137149	39.0	35.0	41.0	28.0	41.0
48	35.73740611787272	39.0	35.0	41.0	28.0	41.0
49	35.662763072579665	39.0	35.0	41.0	28.0	41.0
50	35.570416661038244	38.0	35.0	40.0	27.0	41.0
51	34.71950941522917	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	23.0
9	62.0
10	66.0
11	71.0
12	61.0
13	74.0
14	74.0
15	113.0
16	181.0
17	279.0
18	504.0
19	994.0
20	2272.0
21	4679.0
22	7500.0
23	9826.0
24	11470.0
25	12891.0
26	14658.0
27	15711.0
28	15923.0
29	16528.0
30	19095.0
31	24297.0
32	31950.0
33	45359.0
34	76873.0
35	90409.0
36	95840.0
37	137750.0
38	229449.0
39	319397.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28360507368734	19.7399836550541	21.865839738480865	13.110571532777696
2	31.51974226316714	30.150177633089736	22.49464736792338	15.835432735819746
3	29.87486322927501	28.74743343824717	26.50540666495563	14.872296667522185
4	27.315815423685315	23.53224749760229	32.222000837509626	16.92993624120277
5	29.291392562374202	31.71181226276189	22.697777222439854	16.299017952424048
6	24.45215726269435	38.306693998297966	23.70920517634981	13.531943562657878
7	73.75023639384565	6.147590809007281	16.13362668683894	3.968546110308122
8	74.16476988747654	12.120925583217387	9.764669926650367	3.949634602655716
9	68.37227640519255	8.21696564859717	11.280967593780817	12.129790352429454
10	43.09983249807508	26.019617312134436	18.00426184333167	12.87628834645882
11	34.069334314930636	23.458458847208526	25.127990382147537	17.3442164557133
12	28.349531940185603	21.401747963635874	27.274699779816018	22.974020316362505
13	27.623633981277607	22.251414986018993	30.22430398897729	19.900647043726106
14	19.79047062637615	29.432637885153117	28.307909738075622	22.468981750395116
15	19.841801861432682	25.48452295721947	32.684826216752896	21.988848964594958
16	22.550706479893016	25.491614772589124	28.106130705534316	23.85154804198355
17	21.96664482837807	29.331326237015226	28.60340204514447	20.09862688946224
18	21.528725229302033	26.265720190736065	28.16885950100636	24.036695078955546
19	23.464706398843695	26.683546312931416	26.456101662861855	23.395645625363034
20	27.617133150522093	26.00197220008375	29.109875859460484	17.271018789933677
21	25.08451079982169	29.858484512826056	27.589610152777965	17.46739453457429
22	23.001458887733186	23.221642869686203	32.12474165529725	21.65215658728336
23	25.502590201137394	26.335371948830865	28.973020708100876	19.189017141930865
24	22.693893609261234	26.895625363033403	28.449408339974873	21.961072687730486
25	21.68626484215645	31.42003471612476	26.176650366748166	20.71705007497062
26	21.413145524051387	26.25626443690986	30.42203055559308	21.90855948344568
27	24.51117129773467	28.365235245646975	27.32037444785152	19.803219008766835
28	20.18778113982358	29.429514109335532	29.844132029339853	20.538572721501033
29	21.94731118885842	24.820087398181794	30.19644328573937	23.036158127220414
30	21.40554715044104	30.95518310391873	27.344182685163922	20.2950870604763
31	23.654074754488104	25.86832525091518	25.474307366032228	25.003292628564484
32	26.393794999257047	27.49235097056559	25.557298491131853	20.556555539045508
33	24.719620013778385	28.30309743478907	25.23546515554715	21.741817395885395
34	22.434873495522027	27.052320712153342	26.09129530319199	24.421510489132636
35	25.66840359858974	26.532254251712168	25.53543206040876	22.263910089289332
36	21.791206824352617	31.923131475502842	24.44211052425401	21.84355117589053
37	21.821093760553296	29.53437166515825	26.783591565467585	21.86094300882087
38	23.452380148320255	27.808274460008914	24.84516203109592	23.89418336057491
39	22.81183725296843	28.669085763687203	25.680729849113188	22.838347134231178
40	22.980774426238366	27.38707128287563	27.62278971754312	22.00936457334288
41	20.17545488930014	29.46041416201759	26.520941117670105	23.84318983101217
42	23.236839616906888	26.51680422537114	27.68560293938862	22.560753218333357
43	22.729859244350187	28.25539653379081	25.673975739237324	23.340768482621677
44	22.563623715030594	26.444788528819785	27.763190776587553	23.228396979562064
45	22.308487214470006	25.64501749314458	26.625376541625577	25.421118750759835
46	23.371921814424077	28.913668967566764	25.445180267192587	22.269228950816572
47	21.135973740020802	28.2824973996677	29.81922624917262	20.762302611138878
48	22.401862783503763	27.093520782396084	27.619750368098988	22.884866066001162
49	21.655027083980602	25.735016007240407	30.179811290170065	22.430145618608925
50	21.348221642869685	26.87477204879169	28.545823258452767	23.231183049885857
51	21.079492496183928	25.665786381012843	27.372549946642533	25.882171176160696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	527.0
1	1305.0
2	2083.0
3	9114.5
4	16146.0
5	10609.5
6	5073.0
7	4743.0
8	4413.0
9	4270.0
10	4127.0
11	4021.0
12	3915.0
13	3783.0
14	3651.0
15	3405.5
16	3160.0
17	3206.5
18	3253.0
19	3553.5
20	3854.0
21	4767.5
22	5681.0
23	6025.5
24	6370.0
25	6717.0
26	9228.0
27	11392.0
28	14222.5
29	17053.0
30	19561.0
31	22069.0
32	24868.5
33	27668.0
34	31451.0
35	35234.0
36	36601.0
37	37968.0
38	39649.5
39	41331.0
40	45671.0
41	50011.0
42	53630.0
43	57249.0
44	63448.5
45	69648.0
46	94760.0
47	119872.0
48	104267.0
49	88662.0
50	89979.0
51	91296.0
52	83717.5
53	76139.0
54	72211.0
55	68283.0
56	65222.5
57	62162.0
58	60526.5
59	58891.0
60	56342.5
61	53794.0
62	48176.5
63	42559.0
64	37313.0
65	32067.0
66	25798.5
67	19530.0
68	16436.5
69	13343.0
70	10752.5
71	8162.0
72	6301.0
73	4440.0
74	3756.5
75	2481.0
76	1889.0
77	1262.5
78	636.0
79	512.5
80	389.0
81	304.0
82	219.0
83	144.5
84	70.0
85	42.0
86	14.0
87	18.0
88	22.0
89	12.5
90	3.0
91	4.0
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1184464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.382321055893097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.87569609551537	19.259012786357456
2	8.30492838821101	4.215967173915454
3	2.9907510253856318	2.2773660817373877
4	1.5293200958661026	1.5527077468201052
5	1.0012388614425918	1.2706883117386363
6	0.6946634936716727	1.0579303093309655
7	0.5558187135216329	0.9875578324835691
8	0.47784727762774476	0.9703098413145521
9	0.4393084628220466	1.003560160132806
>10	7.294529818860834	46.656315113107354
>50	0.7728175428958824	11.882989542064001
>100	0.05127376510847592	2.4366173354731293
>500	0.007083875442615895	1.2189936399298664
>1k	0.003373274020293284	1.627836417949471
>5k	6.746548040586567E-4	1.637426028659041
>10k+	6.746548040586567E-4	1.944721678986237
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	11676	0.9857623363816882	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	11037	0.9318138837482608	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	9931	0.8384383147145038	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9193	0.7761316511097003	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	3102	0.26189061043645057	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAAGAT	2482	0.20954625889853976	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	2175	0.1836273622499291	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	1937	0.16353388536924718	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTA	1777	0.15002566561752828	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	1707	0.14411581947615124	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1631	0.13769941509408476	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1550	0.13086087884477707	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1439	0.12148955139202205	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	1212	0.10232476461927081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5327912034472976E-4	0.0	0.0	0.0683009361196288	0.0
2	2.5327912034472976E-4	0.0	0.0	0.44610895730051736	0.0
3	2.5327912034472976E-4	0.0	0.0	0.5790804954815005	0.0
4	2.5327912034472976E-4	0.0	0.0	0.8651170487241486	0.0
5	2.5327912034472976E-4	0.0	0.0	2.0713166461792	0.0
6	2.5327912034472976E-4	0.0	0.0	2.4326615245376813	0.0
7	2.5327912034472976E-4	0.0	0.0	2.907137743316808	0.0
8	2.5327912034472976E-4	0.0	0.0	3.446115711410393	0.0
9	2.5327912034472976E-4	0.0	0.0	3.645530805495144	0.0
10	2.5327912034472976E-4	0.0	0.0	5.0885463804725175	0.0
11	2.5327912034472976E-4	0.0	0.0	5.607768577179214	0.0
12	2.5327912034472976E-4	0.0	0.0	7.015662780802118	0.0
13	2.5327912034472976E-4	0.0	0.0	7.233060692431344	0.0
14	2.5327912034472976E-4	0.0	0.0	7.349737940536817	0.0
15	2.5327912034472976E-4	0.0	0.0	7.649789271771874	0.0
16	2.5327912034472976E-4	0.0	0.0	7.928396304151076	0.0
17	2.5327912034472976E-4	0.0	0.0	8.236468140863716	0.0
18	2.5327912034472976E-4	0.0	0.0	8.55678180172635	0.0
19	2.5327912034472976E-4	0.0	0.0	9.018087506247552	0.0
20	2.5327912034472976E-4	0.0	0.0	9.286647800186413	0.0
21	2.5327912034472976E-4	0.0	0.0	9.542122006240797	0.0
22	2.5327912034472976E-4	0.0	0.0	9.84867416823137	0.0
23	2.5327912034472976E-4	0.0	0.0	10.122300044577125	0.0
24	2.5327912034472976E-4	0.0	0.0	10.354979129800483	0.0
25	3.3770549379297303E-4	0.0	0.0	10.57330573153764	0.0
26	3.3770549379297303E-4	0.0	0.0	10.7674863904686	0.0
27	3.3770549379297303E-4	0.0	0.0	10.999490064704373	0.0
28	3.3770549379297303E-4	0.0	0.0	11.210555998324981	0.0
29	3.3770549379297303E-4	0.0	0.0	11.417400613273177	0.0
30	3.3770549379297303E-4	0.0	0.0	11.664685461103081	0.0
31	3.3770549379297303E-4	0.0	0.0	11.884025179321617	0.0
32	3.3770549379297303E-4	0.0	0.0	12.106573099731186	0.0
33	3.3770549379297303E-4	0.0	0.0	12.325490686082482	0.0
34	4.221318672412163E-4	0.0	0.0	12.54618122627619	0.0
35	4.221318672412163E-4	0.0	0.0	12.779198016993341	0.0
36	4.221318672412163E-4	0.0	0.0	13.007064798930148	0.0
37	4.221318672412163E-4	0.0	0.0	13.251901281930055	0.0
38	4.221318672412163E-4	0.0	0.0	13.488886112199273	0.0
39	5.065582406894595E-4	0.0	0.0	13.742080806170554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTC	20	7.033373E-4	45.000004	26
TGCGCAA	20	7.033373E-4	45.000004	29
CGATCGA	290	0.0	45.000004	41
CCGCGTA	30	2.1655596E-6	44.999996	40
ACGATCG	195	0.0	43.846153	40
CCGATCG	125	0.0	43.199997	40
CGGCGAA	540	0.0	42.916668	31
CGATGAA	3360	0.0	42.656246	19
CCGATGA	3265	0.0	42.243492	18
GGCGAAC	520	0.0	41.971157	32
GATGAAT	3665	0.0	41.930424	20
TACGGCT	1410	0.0	41.648937	7
TCGAATG	635	0.0	41.102364	44
CGAATGC	610	0.0	40.942623	45
ATCGAAT	705	0.0	40.851067	43
CGTTTTT	3600	0.0	40.8125	1
TGAATGA	3830	0.0	40.65274	22
ACGGGAC	340	0.0	40.367645	5
ACCGATG	3440	0.0	40.356102	17
ACGGCTG	1465	0.0	40.085323	8
>>END_MODULE