FastQCFastQC Report
Sat 18 Jun 2016
SRR3551373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692328
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38210.5519060329785882No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC33060.4775193260997677No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG31810.4594643001583065No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGC26650.38493315307195436No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC26400.3813221478836621No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC15160.21897135461804232No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT13290.19196103580961627Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCC11700.1689950428120775No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT8300.11988537225130286No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8160.1178632093458592No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT7580.10948567730902116No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7490.10818571544123595No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC7450.10760795461110918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG253.8891914E-545.00000442
CGACGGT551.8189894E-1245.00000428
CACGACC253.8891914E-545.00000427
TACGGAT351.2109922E-745.00000418
ACGGGCG502.1827873E-1145.0000045
CGTTATC253.8891914E-545.00000431
ATCCCGC253.8891914E-545.00000411
CATCGGC253.8891914E-545.00000415
CGGTCTA551.8189894E-1245.00000431
CGCACTA351.2109922E-745.00000411
GTAACGG600.045.0000042
TCGCACG207.03134E-445.01
CGTCGTC207.03134E-445.016
GACGAAC207.03134E-445.020
CGTTTCA207.03134E-445.029
TCGAACG207.03134E-445.01
TAACGGG1800.042.53
CGGGTAT800.042.18756
CTACGGG1200.041.2500043
CGTTTTT20050.040.1745641